LOCUS       sequence-370908-       10091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sTALED for plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Mok YG, Hong S, Bae SJ, Cho SI, Kim JS
  TITLE     Targeted A-to-G base editing of chloroplast DNA in plants.
  JOURNAL   Nat Plants. 2022 Dec 1. doi: 10.1038/s41477-022-01279-8.
  PUBMED    36456803
REFERENCE   2  (bases 1 to 10091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2022 Dec 1. doi: 10.1038/s41477-022-01279-8."
COMMENT     Sequence Label: pRPS5A-PTP-3Xflag
FEATURES             Location/Qualifiers
     source          1..10091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(5..25)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             1857..1922
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2278..2302
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3633..4262
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4691..5764
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5830..6024
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6260..6282)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6368..6508
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6423..6442
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6523..6540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6694..7282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6774..6793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7528..8319)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8840..8864
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8957..9209)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9229..9780)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(9822..10001)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 cgttcaactt ttctatacaa agttgaagat ctgactcaac ttttgattcg ctatttgcag
       61 tgcacctgtg gcgttcatca catcttttgt gacactgttt gcactggtca ttgctattac
      121 aaaggacctt cctgatgttg aaggagatcg aaagtaagta actgcacgca taaccatttt
      181 ctttccgctc tttggctcaa tccatttgac agtcaaagac aatgtttaac cagctccgtt
      241 tgatatattg tctttatgtg tttgttcaag catgtttagt taatcatgcc tttgattgat
      301 cttgaatagg ttccaaatat caaccctggc aacaaaactt ggagtgagaa acattgcatt
      361 cctcggttct ggacttctgc tagtaaatta tgtttcagcc atatcactag ctttctacat
      421 gcctcaggtg aattcatcta tttccgtctt aactatttcg gttaattaaa gcacgaacac
      481 cattactgca tgtagaagct tgataaacta tcgccaccaa tttatttttg ttgcgatatt
      541 gttactttcc tcagtatgca gctttgaaaa gaccaaccct cttatccttt aacaatgaac
      601 aggtttttag aggtagcttg atgattcctg cacatgtgat cttggcttca ggcttaattt
      661 tccaggtaaa gcattatgag atactcttat atctcttaca tacttttgag ataatgcaca
      721 agaacttcat aactatatgc tttagtttct gcatttgaca ctgccaaatt cattaatctc
      781 taatatcttt gttgttgatc tttggtagac atgggtacta gaaaaagcaa actacaccaa
      841 ggtaaaatac ttttgtacaa acataaactc gttatcacgg aacatcaatg gagtgtatat
      901 ctaacggagt gtagaaacat ttgattattg caggaagcta tctcaggata ttatcggttt
      961 atatggaatc tcttctacgc agagtatctg ttattcccct tcctctagct ttcaatttca
     1021 tggtgaggat atgcagtttt ctttgtatat cattcttctt cttctttgta gcttggagtc
     1081 aaaatcggtt ccttcatgta catacatcaa ggatatgtcc ttctgaattt ttatatcttg
     1141 caataaaaat gcttgtacca attgaaacac cagctttttg agttctatga tcactgactt
     1201 ggttctaacc aaaaaaaaaa aaatgtttaa tttacatatc taaaagtagg tttagggaaa
     1261 cctaaacagt aaaatatttg tatattattc gaatttcact catcataaaa acttaaattg
     1321 caccataaaa ttttgtttta ctattaatga tgtaatttgt gtaacttaag ataaaaataa
     1381 tattccgtaa gttaaccggc taaaaccacg tataaaccag ggaacctgtt aaaccggttc
     1441 tttactggat aaagaaatga aagcccatgt agacagctcc attagagccc aaaccctaaa
     1501 tttctcatct atataaaagg agtgacatta gggtttttgt tcgtcctctt aaagcttctc
     1561 gttttctctg ccgtctctct cattcgcgcg acgcaaacga tcttcaggtg atcttctttc
     1621 tccaaatcct ctctcataac tctgatttcg tacttgtgta tttgagctca cgctctgttt
     1681 ctctcaccac agccatggat tcacagctag tcttgtctct gaagctgaat ccaagcttca
     1741 ctcctctttc tcctctcttc cctttcactc catgttcttc tttttcgccg tcgctccggt
     1801 tttcttcttg ctactcccgc cgcctctatt ctccggttac cgtctacgcc gcgaaggact
     1861 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca
     1921 agcccgggtc aggatcctct ggtacctgat aatcgatatc ccgcggccat gctagagtcc
     1981 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg
     2041 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
     2101 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     2161 taattcctaa aaccaaaatc cagtgacctg caggcatgcg acgtccgtcg ggcccaagct
     2221 tagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
     2281 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
     2341 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     2401 ttcccctcgg gatcaaagta ctttaaagta ctttaaagta ctttaaagta ctttgatcca
     2461 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa
     2521 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
     2581 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
     2641 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
     2701 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
     2761 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
     2821 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
     2881 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
     2941 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
     3001 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
     3061 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
     3121 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
     3181 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
     3241 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
     3301 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
     3361 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
     3421 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
     3481 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
     3541 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
     3601 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
     3661 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
     3721 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
     3781 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
     3841 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
     3901 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
     3961 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
     4021 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
     4081 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
     4141 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
     4201 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
     4261 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
     4321 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
     4381 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
     4441 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
     4501 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
     4561 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
     4621 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg
     4681 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
     4741 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
     4801 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
     4861 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
     4921 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
     4981 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
     5041 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
     5101 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
     5161 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
     5221 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
     5281 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
     5341 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
     5401 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
     5461 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
     5521 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
     5581 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
     5641 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
     5701 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
     5761 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
     5821 tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
     5881 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
     5941 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
     6001 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
     6061 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
     6121 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
     6181 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
     6241 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     6301 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     6361 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     6421 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     6481 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     6541 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6601 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6661 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6721 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6781 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6841 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6901 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6961 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7021 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7081 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     7141 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7201 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7261 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7321 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat gatatatctc ccaatttgtg
     7381 tagggcttat tatgcacgct taaaaataat aaaagcagac ttgacctgat agtttggctg
     7441 tgagcaatta tgtgcttagt gcatctaacg cttgagttaa gccgcgccgc gaagcggcgt
     7501 cggcttgaac gaatttctag ctagacatta tttgccgact accttggtga tctcgccttt
     7561 cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg
     7621 tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
     7681 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
     7741 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
     7801 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
     7861 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
     7921 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
     7981 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
     8041 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
     8101 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
     8161 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
     8221 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
     8281 tagttgagtc gatacttcgg cgatcaccgc ttcccccatg atgtttaact ttgttttagg
     8341 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
     8401 gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa aacagtcata
     8461 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
     8521 ccagttgcgt gagcgcatac gctacttgca ttacagctta cgaaccgaac aggcttatgt
     8581 ccactgggtt cgtgcccgaa ttgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     8641 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     8701 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
     8761 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
     8821 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
     8881 aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttgaattatc
     8941 agcttgcatg ccggtcgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     9001 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     9061 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     9121 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
     9181 aaactttatt gccaaatgtt tgaacgatct gcttgactct aggggtcatc agatttcggt
     9241 gacgggcagg accggacggg gcggcaccgg caggctgaag tccagctgcc agaaacccac
     9301 gtcatgccag ttcccgtgct tgaagccggc cgcccgcagc atgccgcggg gggcatatcc
     9361 gagcgcctcg tgcatgcgca cgctcgggtc gttgggcagc ccgatgacag cgaccacgct
     9421 cttgaagccc tgtgcctcca gggacttcag aaggtgggtg tagagcgtgg agcccagtcc
     9481 cgtccgctgg tggcgggggg agacgtacac ggtcgactcg gccgtccagt cgtaggcgtt
     9541 gcgtgccttc cagggacccg cgtaggcgat gccggcgacc tcgccgtcca cctcggcgac
     9601 gagccaggga tagcgctccc gcagacggac gaggtcgtcc gtccactcct gcggttcctg
     9661 cggctcggta cggaagttga ccgtgcttgt ctcgatgtag tggttgacga tggtgcagac
     9721 cgccggcatg tccgcctcgg tggcacggcg gatgtcggcc gggcgtcgtt ctgggctcat
     9781 ggtagatccc ctcgatcgag ttgagagtga atatgagact ctaattggat accgagggga
     9841 atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg atagtgacct
     9901 taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct cattaaactc
     9961 cagaaacccg cggctcagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa
    10021 acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctccgctcat
    10081 gatcgataat t
//
