LOCUS       sequence-370911-       13154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  16S rRNA Left_G1397C-ABE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13154)
  AUTHORS   Mok YG, Hong S, Bae SJ, Cho SI, Kim JS
  TITLE     Targeted A-to-G base editing of chloroplast DNA in plants.
  JOURNAL   Nat Plants. 2022 Dec 1. doi: 10.1038/s41477-022-01279-8.
  PUBMED    36456803
REFERENCE   2  (bases 1 to 13154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2022 Dec 1. doi: 10.1038/s41477-022-01279-8."
COMMENT     Sequence Label: pRPS5A-PTP-3Xflag-16S rRNA Left_G1397C-ABE
FEATURES             Location/Qualifiers
     source          1..13154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(5..25)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             1857..1922
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5341..5365
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6696..7325
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7754..8827
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8893..9087
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9323..9345)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9431..9571
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9486..9505
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9586..9603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9757..10345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9837..9856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10591..11382)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    11903..11927
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(12020..12272)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12292..12843)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(12885..13064)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 cgttcaactt ttctatacaa agttgaagat ctgactcaac ttttgattcg ctatttgcag
       61 tgcacctgtg gcgttcatca catcttttgt gacactgttt gcactggtca ttgctattac
      121 aaaggacctt cctgatgttg aaggagatcg aaagtaagta actgcacgca taaccatttt
      181 ctttccgctc tttggctcaa tccatttgac agtcaaagac aatgtttaac cagctccgtt
      241 tgatatattg tctttatgtg tttgttcaag catgtttagt taatcatgcc tttgattgat
      301 cttgaatagg ttccaaatat caaccctggc aacaaaactt ggagtgagaa acattgcatt
      361 cctcggttct ggacttctgc tagtaaatta tgtttcagcc atatcactag ctttctacat
      421 gcctcaggtg aattcatcta tttccgtctt aactatttcg gttaattaaa gcacgaacac
      481 cattactgca tgtagaagct tgataaacta tcgccaccaa tttatttttg ttgcgatatt
      541 gttactttcc tcagtatgca gctttgaaaa gaccaaccct cttatccttt aacaatgaac
      601 aggtttttag aggtagcttg atgattcctg cacatgtgat cttggcttca ggcttaattt
      661 tccaggtaaa gcattatgag atactcttat atctcttaca tacttttgag ataatgcaca
      721 agaacttcat aactatatgc tttagtttct gcatttgaca ctgccaaatt cattaatctc
      781 taatatcttt gttgttgatc tttggtagac atgggtacta gaaaaagcaa actacaccaa
      841 ggtaaaatac ttttgtacaa acataaactc gttatcacgg aacatcaatg gagtgtatat
      901 ctaacggagt gtagaaacat ttgattattg caggaagcta tctcaggata ttatcggttt
      961 atatggaatc tcttctacgc agagtatctg ttattcccct tcctctagct ttcaatttca
     1021 tggtgaggat atgcagtttt ctttgtatat cattcttctt cttctttgta gcttggagtc
     1081 aaaatcggtt ccttcatgta catacatcaa ggatatgtcc ttctgaattt ttatatcttg
     1141 caataaaaat gcttgtacca attgaaacac cagctttttg agttctatga tcactgactt
     1201 ggttctaacc aaaaaaaaaa aaatgtttaa tttacatatc taaaagtagg tttagggaaa
     1261 cctaaacagt aaaatatttg tatattattc gaatttcact catcataaaa acttaaattg
     1321 caccataaaa ttttgtttta ctattaatga tgtaatttgt gtaacttaag ataaaaataa
     1381 tattccgtaa gttaaccggc taaaaccacg tataaaccag ggaacctgtt aaaccggttc
     1441 tttactggat aaagaaatga aagcccatgt agacagctcc attagagccc aaaccctaaa
     1501 tttctcatct atataaaagg agtgacatta gggtttttgt tcgtcctctt aaagcttctc
     1561 gttttctctg ccgtctctct cattcgcgcg acgcaaacga tcttcaggtg atcttctttc
     1621 tccaaatcct ctctcataac tctgatttcg tacttgtgta tttgagctca cgctctgttt
     1681 ctctcaccac agccatggat tcacagctag tcttgtctct gaagctgaat ccaagcttca
     1741 ctcctctttc tcctctcttc cctttcactc catgttcttc tttttcgccg tcgctccggt
     1801 tttcttcttg ctactcccgc cgcctctatt ctccggttac cgtctacgcc gcgaaggact
     1861 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca
     1921 agcccgggga tctacgcacg ctcggctaca gccagcagca acaggagaag atcaaaccga
     1981 aggttcgttc gacagtggcg cagcaccacg aggcactggt cggccatggg tttacacacg
     2041 cgcacatcgt tgcgctcagc caacacccgg cagcgttagg gaccgtcgct gtcaagtatc
     2101 aggacatgat cgcagcgttg ccagaggcga cacacgaagc gatcgttggc gtcggcaaac
     2161 agtggtccgg cgcacgcgct ctggaggcct tgctcacggt ggcgggagag ttgagaggtc
     2221 caccgttaca gttggacaca ggccaacttc tcaagattgc aaaacgtggc ggcgtgaccg
     2281 cagtggaggc agtgcatgca tggcgcaatg cactgacggg tgcccccctg aacttgaccc
     2341 ctgcacaagt ggtggctatc gccagcaaca tcggtggtaa gcaagcattg gaaactgtgc
     2401 aacgcctttt gccagtcctt tgtcaggatc atggtcttac acccgaccag gtggtcgcaa
     2461 tcgcctctaa tattgggggg aagcaggcct tggaaactgt tcaaagattg ctcccagtcc
     2521 tctgccagga tcacggcctg acccccgatc aagtcgttgc cattgctagt aacaatggcg
     2581 gaaaacaagc tctcgagaca gtgcagcggc tcctccctgt gctctgccaa gcacacgggt
     2641 tgacccccgc tcaggttgtg gctatcgcaa gtaacaacgg tggaaaacag gcccttgaaa
     2701 ctgtccaacg ccttcttccc gttctctgcc aggctcacgg cttgacaccc gaccaggtcg
     2761 ttgctattgc ctcaaacaat ggaggaaaac aggcactcga aaccgtccag cggctgctcc
     2821 ccgtcttgtg ccaggaccat ggactgacac cagagcaagt ggttgccatc gcctccaaca
     2881 acggtggtaa gcaagcactt gagactgtgc agaggctttt gcctgtgctt tgtcaggccc
     2941 atgggctcac accagcccag gtcgttgcca ttgcaagtca tgacggcggc aagcaagcat
     3001 tggagacagt gcagagactc ttgcccgtcc tgtgtcaaga ccatgggttg actcctgatc
     3061 aggtcgtcgc aattgcctcc aatatcgggg gcaaacaggc cttggagaca gtccaacgct
     3121 tgctcccagt tctttgccaa gatcacggcc ttacacccgc acaggtggtc gccatcgcta
     3181 gtaatggtgg gggcaaacag gctctcgaga ctgttcaacg gcttctccca gttttgtgtc
     3241 aagcacatgg gcttacccca gcacaggttg tcgcaattgc ctccaataac ggtggtaaac
     3301 aggctctgga aacagtccag agactcctcc ccgttttgtg tcaagaccac ggtctgaccc
     3361 ccgcccaagt ggttgctatt gccagtaaca ttggtggaaa acaagccctc gaaactgttc
     3421 agaggctttt gccagtcctg tgccaggccc atgggcttac acctgagcaa gtggttgcaa
     3481 tcgcttctaa cggagggggc aagcaggctc ttgagacagt tcaacgcctg ttgccagttt
     3541 tgtgccaggc tcacggtctg actcccgctc aagttgtcgc tatcgccagc aataatggag
     3601 gcaaacaagc acttgaaaca gtgcagagat tgttgccagt gctgtgccaa gaccacggct
     3661 tgacacccgc ccaagttgtt gctattgcca gtaatatagg gggtaagcag gctctcgaaa
     3721 cagttcaacg cctccttcct gttttgtgtc aggctcatgg tcttacaccc gatcaggttg
     3781 ttgcaatcgc atcacacgat ggcgggaaac aggccctcga aaccgtccag cggcttttgc
     3841 ctgtgctgtg ccaagctcat ggcttgacac ccgaacaggt tgttgccatt gccagtaacg
     3901 ggggcggtaa acaggcactt gaaacagtgc agagattgct cccagttttg tgccaagcac
     3961 acgggttgac acctgcacag gtggtggcaa tcgcatctaa cggcggagga aagcaggctc
     4021 tggagactgt tcaaaggctt cttcccgttt tgtgtcaggc tcatggactt actccagacc
     4081 aagttgtggc cattgcaagc aacaatggcg ggaaacaggc cttggagact gttcaaagac
     4141 tcctgcccgt cctgtgtcaa gatcacggtc tgactccgga acaggtggtg gcgattgcaa
     4201 gcaacggcgg cggcaaacag gctctagaga gcattgttgc ccagctctcc agacctgatc
     4261 cggcgctagc cgcgttgaca aatgatcatc ttgttgctct tgcttgtctt ggcggacgac
     4321 ctgccctgga tgctgtgaaa aaggggttgg ggggatccgg cagtgccata cctgtgaagc
     4381 ggggagcaac aggggagaca aaggtgttca caggcaactc taacagtcca aagagcccca
     4441 ccaaaggcgg gtgttcagga agcgaaactc ctggtacctc agagtccgct actcccgaat
     4501 cctctgaggt ggagttttcc cacgagtact ggatgagaca tgccctgacc ctggccaaga
     4561 gggcacggga tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga
     4621 tcggcgaggg ctggaacaga gccatcggcc tgcacgaccc aacagcccat gccgaaatta
     4681 tggccctgag acagggcggc ctggtcatgc agaactacag actgattgac gccaccctgt
     4741 acgtgacatt cgagccttgc gtgatgtgcg ccggcgccat gatccactct aggatcggcc
     4801 gcgtggtgtt tggcgtgagg aactcaaaaa gaggcgccgc aggctccctg atgaacgtgc
     4861 tgaactaccc cggcatgaat caccgcgtcg aaattaccga gggaatcctg gcagatgaat
     4921 gtgccgccct gctgtgcgat ttctatcgga tgcctagaca ggtgttcaat gctcagaaga
     4981 aggcccagag ctccatcaac tagggtacct gataatcgat atcccgcggc catgctagag
     5041 tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata
     5101 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt
     5161 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     5221 ttctaattcc taaaaccaaa atccagtgac ctgcaggcat gcgacgtccg tcgggcccaa
     5281 gcttagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt
     5341 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
     5401 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
     5461 gggttcccct cgggatcaaa gtactttaaa gtactttaaa gtactttaaa gtactttgat
     5521 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
     5581 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
     5641 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
     5701 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
     5761 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
     5821 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
     5881 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
     5941 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
     6001 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     6061 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     6121 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     6181 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     6241 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     6301 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     6361 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     6421 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     6481 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     6541 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     6601 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     6661 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     6721 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     6781 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     6841 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     6901 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     6961 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     7021 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     7081 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     7141 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     7201 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     7261 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     7321 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     7381 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     7441 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     7501 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     7561 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     7621 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     7681 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
     7741 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
     7801 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
     7861 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
     7921 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca
     7981 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
     8041 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
     8101 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
     8161 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
     8221 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
     8281 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
     8341 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
     8401 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
     8461 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg
     8521 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
     8581 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
     8641 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
     8701 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
     8761 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
     8821 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
     8881 aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
     8941 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
     9001 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
     9061 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca
     9121 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
     9181 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
     9241 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
     9301 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     9361 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     9421 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     9481 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     9541 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     9601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    10201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10381 tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat ctcccaattt
    10441 gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg
    10501 ctgtgagcaa ttatgtgctt agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg
    10561 cgtcggcttg aacgaatttc tagctagaca ttatttgccg actaccttgg tgatctcgcc
    10621 tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc
    10681 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
    10741 ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta
    10801 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga
    10861 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc
    10921 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag
    10981 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt
    11041 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
    11101 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
    11161 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
    11221 caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc
    11281 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
    11341 tgatagttga gtcgatactt cggcgatcac cgcttccccc atgatgttta actttgtttt
    11401 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
    11461 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaacagtc
    11521 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
    11581 ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg aacaggctta
    11641 tgtccactgg gttcgtgccc gaattgatca caggcagcaa cgctctgtca tcgttacaat
    11701 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
    11761 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
    11821 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
    11881 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
    11941 gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc gaattgaatt
    12001 atcagcttgc atgccggtcg atctagtaac atagatgaca ccgcgcgcga taatttatcc
    12061 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
    12121 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
    12181 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
    12241 aagaaacttt attgccaaat gtttgaacga tctgcttgac tctaggggtc atcagatttc
    12301 ggtgacgggc aggaccggac ggggcggcac cggcaggctg aagtccagct gccagaaacc
    12361 cacgtcatgc cagttcccgt gcttgaagcc ggccgcccgc agcatgccgc ggggggcata
    12421 tccgagcgcc tcgtgcatgc gcacgctcgg gtcgttgggc agcccgatga cagcgaccac
    12481 gctcttgaag ccctgtgcct ccagggactt cagaaggtgg gtgtagagcg tggagcccag
    12541 tcccgtccgc tggtggcggg gggagacgta cacggtcgac tcggccgtcc agtcgtaggc
    12601 gttgcgtgcc ttccagggac ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc
    12661 gacgagccag ggatagcgct cccgcagacg gacgaggtcg tccgtccact cctgcggttc
    12721 ctgcggctcg gtacggaagt tgaccgtgct tgtctcgatg tagtggttga cgatggtgca
    12781 gaccgccggc atgtccgcct cggtggcacg gcggatgtcg gccgggcgtc gttctgggct
    12841 catggtagat cccctcgatc gagttgagag tgaatatgag actctaattg gataccgagg
    12901 ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa tatttgctag ctgatagtga
    12961 ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta gctcattaaa
    13021 ctccagaaac ccgcggctca gtggctcctt caacgttgcg gttctgtcag ttccaaacgt
    13081 aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta attctccgct
    13141 catgatcgat aatt
//
