LOCUS       sequence-370916-       12836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psa A Right _G1397N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12836)
  AUTHORS   Mok YG, Hong S, Bae SJ, Cho SI, Kim JS
  TITLE     Targeted A-to-G base editing of chloroplast DNA in plants.
  JOURNAL   Nat Plants. 2022 Dec 1. doi: 10.1038/s41477-022-01279-8.
  PUBMED    36456803
REFERENCE   2  (bases 1 to 12836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2022 Dec 1. doi: 10.1038/s41477-022-01279-8."
COMMENT     Sequence Label: AatII-pRPS5A-PTP-3Xflag- psa A Right _G1397N
FEATURES             Location/Qualifiers
     source          1..12836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(5..25)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             1857..1922
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5023..5047
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6378..7007
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7436..8509
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8575..8769
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9005..9027)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9113..9253
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9168..9187
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9268..9285)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9439..10027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9519..9538)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10273..11064)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    11585..11609
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(11702..11954)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11974..12525)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(12567..12746)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 cgttcaactt ttctatacaa agttgagacg tcgactcaac ttttgattcg ctatttgcag
       61 tgcacctgtg gcgttcatca catcttttgt gacactgttt gcactggtca ttgctattac
      121 aaaggacctt cctgatgttg aaggagatcg aaagtaagta actgcacgca taaccatttt
      181 ctttccgctc tttggctcaa tccatttgac agtcaaagac aatgtttaac cagctccgtt
      241 tgatatattg tctttatgtg tttgttcaag catgtttagt taatcatgcc tttgattgat
      301 cttgaatagg ttccaaatat caaccctggc aacaaaactt ggagtgagaa acattgcatt
      361 cctcggttct ggacttctgc tagtaaatta tgtttcagcc atatcactag ctttctacat
      421 gcctcaggtg aattcatcta tttccgtctt aactatttcg gttaattaaa gcacgaacac
      481 cattactgca tgtagaagct tgataaacta tcgccaccaa tttatttttg ttgcgatatt
      541 gttactttcc tcagtatgca gctttgaaaa gaccaaccct cttatccttt aacaatgaac
      601 aggtttttag aggtagcttg atgattcctg cacatgtgat cttggcttca ggcttaattt
      661 tccaggtaaa gcattatgag atactcttat atctcttaca tacttttgag ataatgcaca
      721 agaacttcat aactatatgc tttagtttct gcatttgaca ctgccaaatt cattaatctc
      781 taatatcttt gttgttgatc tttggtagac atgggtacta gaaaaagcaa actacaccaa
      841 ggtaaaatac ttttgtacaa acataaactc gttatcacgg aacatcaatg gagtgtatat
      901 ctaacggagt gtagaaacat ttgattattg caggaagcta tctcaggata ttatcggttt
      961 atatggaatc tcttctacgc agagtatctg ttattcccct tcctctagct ttcaatttca
     1021 tggtgaggat atgcagtttt ctttgtatat cattcttctt cttctttgta gcttggagtc
     1081 aaaatcggtt ccttcatgta catacatcaa ggatatgtcc ttctgaattt ttatatcttg
     1141 caataaaaat gcttgtacca attgaaacac cagctttttg agttctatga tcactgactt
     1201 ggttctaacc aaaaaaaaaa aaatgtttaa tttacatatc taaaagtagg tttagggaaa
     1261 cctaaacagt aaaatatttg tatattattc gaatttcact catcataaaa acttaaattg
     1321 caccataaaa ttttgtttta ctattaatga tgtaatttgt gtaacttaag ataaaaataa
     1381 tattccgtaa gttaaccggc taaaaccacg tataaaccag ggaacctgtt aaaccggttc
     1441 tttactggat aaagaaatga aagcccatgt agacagctcc attagagccc aaaccctaaa
     1501 tttctcatct atataaaagg agtgacatta gggtttttgt tcgtcctctt aaagcttctc
     1561 gttttctctg ccgtctctct cattcgcgcg acgcaaacga tcttcaggtg atcttctttc
     1621 tccaaatcct ctctcataac tctgatttcg tacttgtgta tttgagctca cgctctgttt
     1681 ctctcaccac agccatggat tcacagctag tcttgtctct gaagctgaat ccaagcttca
     1741 ctcctctttc tcctctcttc cctttcactc catgttcttc tttttcgccg tcgctccggt
     1801 tttcttcttg ctactcccgc cgcctctatt ctccggttac cgtctacgcc gcgaaggact
     1861 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca
     1921 agcccgggga tctacgcacg ctcggctaca gccagcagca acaggagaag atcaaaccga
     1981 aggttcgttc gacagtggcg cagcaccacg aggcactggt cggccatggg tttacacacg
     2041 cgcacatcgt tgcgctcagc caacacccgg cagcgttagg gaccgtcgct gtcaagtatc
     2101 aggacatgat cgcagcgttg ccagaggcga cacacgaagc gatcgttggc gtcggcaaac
     2161 agtggtccgg cgcacgcgct ctggaggcct tgctcacggt ggcgggagag ttgagaggtc
     2221 caccgttaca gttggacaca ggccaacttc tcaagattgc aaaacgtggc ggcgtgaccg
     2281 cagtggaggc agtgcatgca tggcgcaatg cactgacggg tgcccccctg aacttgacac
     2341 ccgaacaagt cgtcgctatt gcaagcaaca acggggggaa acaggccctg gagactgtcc
     2401 agcggctgtt gcctgttctc tgtcaggcac atggcctcac ccctgaccaa gtggtggcta
     2461 tcgcatctaa cggcggtggc aagcaggcct tggaaacagt gcaaaggctt ctgccagtct
     2521 tgtgccagga tcatgggctc acaccagctc aggttgttgc aatcgccagt aataacggag
     2581 gtaagcaagc tctggaaacc gtgcagaggt tgctgcctgt tctgtgtcaa gcacacgggt
     2641 tgacacccga acaggttgtt gccattgcca gtaacggggg cggtaaacag gcacttgaaa
     2701 cagtgcagag attgctccca gttttgtgcc aagcacacgg gttgacacct gcacaggtgg
     2761 tggcaatcgc atctaacggc ggaggaaagc aggctctgga gactgttcaa aggcttcttc
     2821 ccgttttgtg tcaggctcat ggacttactc cagaccaagt tgtggccatt gcaagcaaca
     2881 atggcgggaa acaggccttg gagactgttc aaagactcct gcccgtcctg tgtcaagatc
     2941 acggcttgac accagcccaa gttgtcgcca ttgccagtaa tggtggtggt aagcaggccc
     3001 tggaaacagt ccaaagattg ttgccagtcc tttgtcaaga ccatgggctc acccctgcac
     3061 aagtcgtggc tatcgccagt catgatgggg ggaagcaagc attggaaact gtccagcggt
     3121 tgcttcccgt tctctgccag gcacacggat tgactcccga acaagttgtt gcaatcgcct
     3181 cacacgacgg gggtaaacag gccctggaga ctgtgcaaag gcttctgcca gttttgtgcc
     3241 aagctcatgg acttacccct gcacaggttg tcgcaatcgc atccaacaat ggaggaaaac
     3301 aagcactcga aaccgttcag cgcctgcttc ctgtcttgtg tcaggcacac ggactgactc
     3361 cagaccaggt ggttgctatc gccagcaata acggaggtaa acaagccttg gagactgtcc
     3421 aaaggttgtt gcccgttctc tgccaagacc atggtttgac accagctcaa gtggttgcca
     3481 tcgcttctaa tatcgggggt aagcaggcac tcgagacagt ccagagactc ctgcctgttc
     3541 tctgccaggc tcacgggttg actcctgcac aagtggtggc tatcgccagc aacatcggtg
     3601 gtaagcaagc attggaaact gtgcaacgcc ttttgccagt cctttgtcag gatcatggtc
     3661 ttacacccga ccaggtggtc gcaatcgcct ctaatattgg ggggaagcag gccttggaaa
     3721 ctgttcaaag attgctccca gtcctctgcc aggatcacgg cctgaccccc gatcaagtcg
     3781 ttgccattgc tagtaacaat ggcggaaaac aagctctcga gacagtgcag cggctcctcc
     3841 ctgtgctctg ccaagctcat gggctcactc ccgcacaggt tgttgctatc gcatccaaca
     3901 tcggtggaaa acaggcattg gaaacagttc agcgcttgct gcctgttctt tgtcaagcac
     3961 atgggcttac accagcccaa gtcgttgcca tcgccagcaa caatgggggc aagcaagctc
     4021 tcgaaaccgt tcagagactt ctgcccgtgt tgtgccaaga tcatgggttg accccagctc
     4081 aggtggtcgc aattgcttca aataacgggg gcaagcaagc actggagact gttcaacgcc
     4141 tcctgcccgt cctctgccaa gcacacggtc tgactccgga acaggtggtg gcgattgcaa
     4201 gcaacggcgg cggcaaacag gctctagaga gcattgttgc ccagctctcc agacctgatc
     4261 cggcgctagc cgcgttgaca aatgatcatc ttgttgctct tgcttgtctt ggcggacgac
     4321 ctgccctgga tgctgtgaaa aaggggttgg ggggatccgg atcaggtagc tacgcacttg
     4381 gtccttacca gattagcgca ccccaactcc ccgcctataa tggtcaaacc gtcgggacct
     4441 tttactacgt aaacgatgct ggtgggctgg aatccaaagt attctcctca gggggcccta
     4501 caccctaccc caactacgcc aatgctggtc atgtagaagg gcagtcagca ctgtttatgc
     4561 gcgataatgg tataagcgag gggttggtct tccataacaa cccagagggt acttgtggct
     4621 tctgtgtgaa tatgactgaa acccttctgc ccgaaaatgc caagatgact gtcgtcccac
     4681 ctgaaggcta gggtacctga taatcgatat cccgcggcca tgctagagtc cgcaaaaatc
     4741 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag
     4801 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga
     4861 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     4921 aaaccaaaat ccagtgacct gcaggcatgc cgtcgggccc aagcttagct tgagcttgga
     4981 tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg
     5041 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     5101 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
     5161 aagtacttta aagtacttta aagtacttta aagtactttg atccaacccc tccgctgcta
     5221 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
     5281 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     5341 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     5401 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     5461 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     5521 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     5581 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     5641 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     5701 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     5761 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     5821 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     5881 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     5941 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     6001 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     6061 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     6121 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     6181 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     6241 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     6301 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     6361 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     6421 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     6481 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     6541 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     6601 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     6661 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     6721 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     6781 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     6841 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     6901 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     6961 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     7021 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     7081 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     7141 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     7201 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     7261 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     7321 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     7381 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
     7441 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     7501 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     7561 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     7621 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     7681 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     7741 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     7801 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     7861 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     7921 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     7981 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     8041 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     8101 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     8161 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     8221 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     8281 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     8341 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     8401 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     8461 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     8521 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     8581 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     8641 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     8701 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     8761 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     8821 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     8881 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     8941 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     9001 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     9061 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     9121 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     9181 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     9241 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9301 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9361 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9421 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9481 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9541 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9601 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9661 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9721 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9781 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9841 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9901 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9961 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10021 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10081 acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc
    10141 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
    10201 ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt
    10261 tctagctaga cattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
    10321 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
    10381 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
    10441 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
    10501 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
    10561 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
    10621 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
    10681 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
    10741 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
    10801 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
    10861 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
    10921 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
    10981 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
    11041 ttcggcgatc accgcttccc ccatgatgtt taactttgtt ttagggcgac tgccctgctg
    11101 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
    11161 cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac
    11221 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
    11281 catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc
    11341 ccgaattgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
    11401 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
    11461 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
    11521 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
    11581 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
    11641 tgcggacgtt tttaatgtac tgaattaacg ccgaattgaa ttatcagctt gcatgccggt
    11701 cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
    11761 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
    11821 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
    11881 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
    11941 atgtttgaac gatctgcttg actctagggg tcatcagatt tcggtgacgg gcaggaccgg
    12001 acggggcggc accggcaggc tgaagtccag ctgccagaaa cccacgtcat gccagttccc
    12061 gtgcttgaag ccggccgccc gcagcatgcc gcggggggca tatccgagcg cctcgtgcat
    12121 gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc acgctcttga agccctgtgc
    12181 ctccagggac ttcagaaggt gggtgtagag cgtggagccc agtcccgtcc gctggtggcg
    12241 gggggagacg tacacggtcg actcggccgt ccagtcgtag gcgttgcgtg ccttccaggg
    12301 acccgcgtag gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc agggatagcg
    12361 ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct cggtacggaa
    12421 gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg cagaccgccg gcatgtccgc
    12481 ctcggtggca cggcggatgt cggccgggcg tcgttctggg ctcatggtag atcccctcga
    12541 tcgagttgag agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca
    12601 gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa
    12661 cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct
    12721 cagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg
    12781 tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatcg ataatt
//
