LOCUS       sequence-370937-       16679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the fluorescently labeled motor-deficient dynein motor 
            domain fused to the dimerization domain FKBP to recruit it to the 
            plasma membrane by using the chemical dimerization system FKBP-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16679)
  AUTHORS   Schelski M, Bradke F
  TITLE     Microtubule retrograde flow retains neuronal polarization in a 
            fluctuating state.
  JOURNAL   Sci Adv. 2022 Nov 4;8(44):eabo2336. doi: 10.1126/sciadv.abo2336. 
            Epub 2022 Nov 4.
  PUBMED    36332023
REFERENCE   2  (bases 1 to 16679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-11-4"; volume: "8(44):eabo2336. doi"; pages: " 
            10.1126/sciadv.abo2336. Epub 2022 Nov 4"
COMMENT     Sequence Label: pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T
FEATURES             Location/Qualifiers
     source          1..16679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    150..284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(187..206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     241..260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(298..314)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(298..314)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(311..333)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    322..338
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(346..352,353..370,371..376))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    391..412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(529..546)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(700..1288)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(780..799)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1459..2250,2251..2319))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2082..2101
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2320..2424)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2492..2510
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2548..2570)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2670..2689
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2883..2905
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2897..2914
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2898..2914
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4715..4990
                     /label=chicken beta-actin promoter
     primer_bind     5933..5955
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             6012..6332
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6354..7049
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(6487..6505)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6755..6774
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      11019..11028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acggtaccgc gggcccggga tccaccggat ctagataagt aatgatcata atcagccata
       61 tcacatctgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      121 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      181 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      241 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaattcg taatcatgtc
      301 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      361 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      421 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      481 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      541 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      601 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      661 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      721 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      781 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      841 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      901 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      961 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1021 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1081 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1141 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1201 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1261 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1321 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1381 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1441 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1501 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1561 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1621 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1681 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1741 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1801 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1861 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1921 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1981 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2041 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2101 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2161 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2221 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2281 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2341 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2401 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     2461 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     2521 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     2581 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     2641 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     2701 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     2761 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     2821 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     2881 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ttgggatctt tgcattggcc
     2941 cacggctctc aggatgggga tgctcccctt cagcacccgg ttccccttgg aaactgatgg
     3001 tcctggctct gtggcatggc agtggcactg tgaggagccc cctaccagca gcacacagtg
     3061 gggttggcac tgccacgctc cggatgccgc gctctgatcc aaccccataa tcaagggaac
     3121 ccgaattgcc ccatcattgc ccccaccacc cccatcctgc cgggccctca caccccacgc
     3181 tgccttctgg tgacattccc cagcccaaac ccacggcttc atggctaccg cggggcattt
     3241 cccattgccg ccccattatc agctctgcac acctcccgct gtacccatgc ctcgtggctg
     3301 cccttctttg acgtataatc ttctaattaa tacccggcct tgtcaaagtg gagcacaaac
     3361 gttaattaat tccccagcag gcaggtaatt aacagtgtga ctcccttttt gctgcgagtg
     3421 gggctgatac agagagatgt ggcactatgg agcccacggg gtcctggcac tgggtgccca
     3481 cggaggtccc catgtgctgc agtgtcaccg cctccgagga gacagtattg tccctgcggt
     3541 gtccctgcag ctcagctctg tccacagggc cacctccagt ttggagggga cacaatgcag
     3601 ccccgatgca acccatcctc gcagcatccc agggacaaag accccactgc aagaccgcac
     3661 acagggctgg gtcccgctcc cctaatatct acagtgcttc ttgcatggcc ccttaatcaa
     3721 tgcagttaat cagcatgcgc tcatgcaccg ctctggagct gcaaagcccc tcgcagcgct
     3781 gctcaccaac accgcgcacc gccccggccc agcctgcagc acgcgctgca aacaggaaag
     3841 aaacaaaata ttgcccaaat gtaggcaaag gcattcggct gccttgacct ccgccgggcc
     3901 gggccctgcc tgactcagct ccttactcag cgctcgcttc ctccctccgg ctgccaccgc
     3961 cgcagcgcac accctgacaa agagtggccc ttaacgggct ctgaggtgca cccagcagtg
     4021 cactcagcag tccaagggcc ggcctggagg tttgcaccgc tacgtgctga cattagcatt
     4081 gaacttggcc ctgggtagtg ctgcaggccg ggcggggtgg gtgtagagag tgcagcgcgc
     4141 gttgcacccg gtgccccttc ccctcccttg catcccagca ggctgcaccc cagcaccagg
     4201 cccgtgcatg catgctcctg gtgttattgc agcctggtgc atgcatgcgt cttgatggtg
     4261 cagcgctgtg catgcatcct ccttggtgtg tagcagtcta gtgcatgcat acccctcggt
     4321 gttattgctg ctctgtgcac gcacgctcat tgtatcactt catcccagtg catgcactca
     4381 cactggagcg attgctgctc ggtgcacgca cactcattgt atcactgcag ctcagtggct
     4441 gcacgcacac cggtgttatt gctgctcggt gcgtgcatgc acatcagtgt cgctgcagct
     4501 cagtgcatgc acgctcattg cccatcgcta tccctgcctc tcctgctggc gctccccggg
     4561 aggtgacttc aaggggaccg caggaccacc tcgggggtgg ggggagggct gcacacgcgg
     4621 accccgctcc ccctccccaa caaagcactg tggaatcaaa aaggggggag gggggatgga
     4681 ggggcgcgtc acacccccgc cccacaccct cacctcgagg tgagccccac gttctgcttc
     4741 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     4801 ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg
     4861 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     4921 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     4981 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
     5041 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     5101 cccttctcct ccgggctgta attagcgctt ggtttaatga cggctcgttt cttttctgtg
     5161 gctgcgtgaa agccttaaag ggctccggga gggccctttg tgcggggggg agcggctcgg
     5221 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
     5281 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
     5341 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
     5401 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcggcggtc gggctgtaac
     5461 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     5521 cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
     5581 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
     5641 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     5701 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
     5761 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
     5821 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctcca tctccagcct
     5881 cggggctgtc cgcaggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     5941 ttctggcgtg tgaccggcgg ggtttatatc ttcccttctc tgttcctccg cagcccccaa
     6001 gctagctcga gggagtgcag gtggaaacca tctccccagg agacgggcgc accttcccca
     6061 agcgcggcca gacctgcgtg gtgcactaca ccgggatgct tgaagatgga aagaaatttg
     6121 attcctcccg ggacagaaac aagcccttta agtttatgct aggcaagcag gaggtgatcc
     6181 gaggctggga agaaggggtt gcccagatga gtgtgggtca gagagccaaa ctgactatat
     6241 ctccagatta tgcctatggt gccactgggc acccaggcat catcccacca catgccactc
     6301 tcgtcttcga tgtggagctt ctaaaactgg aaggaagttc aggaggttct agtatggtga
     6361 gcaagggcga ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg gagggctcca
     6421 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     6481 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttctcctgg gacatcctgt
     6541 cccctcagtt catgtacggc tccagggcct tcatcaagca ccccgccgac atccccgact
     6601 actataagca gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     6661 gcggcgccgt gaccgtgacc caggacacct ccctggagga cggcaccctg atctacaagg
     6721 tgaagctccg cggcaccaac ttccctcctg acggccccgt aatgcagaag aagacaatgg
     6781 gctgggaagc gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta
     6841 agatggccct gcgcctgaag gacggcggcc gctacctggc ggacttcaag accacctaca
     6901 aggccaagaa gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag ttggacatca
     6961 cctcccacaa cgaggactac accgtggtgg aacagtacga acgctccgag ggccgccact
     7021 ccaccggcgg catggacgag ctgtacaagt ctggtggatc aagtggtgga ggcgagatgg
     7081 ccttggaaga atttttgaag cagatacgag aagtgtggaa cacatacgaa ctagacttgg
     7141 tcaactatca gaacaaatgc cgcctcatcc gaggctggga tgacctcttc aacaaggtca
     7201 aggagcatat caacagcgtc tctgccatga aactctctcc ctattacaag gtttttgaag
     7261 aagatgccct gagctgggag gacaagctga accgaatcat ggctctcttt gacgtgtgga
     7321 ttgacgtgca gaggcgctgg gtctacttgg aaggcatctt cacaggcagt gcggacatca
     7381 aacatctgct gcctgtggaa acccagcgct tccagagtat cagcacggag tttttggctc
     7441 tgatgaaaaa agtctccaag tctccacttg tgatggatgt tctgaacata cagggagtcc
     7501 agaggtctct ggaaaggttg gcagacctgc tggggaagat ccagaaagca ttgggggagt
     7561 acctggaacg ggagcgctcg tctttcccca ggttctactt tgtgggtgat gaggatttgc
     7621 ttgagatcat tgggaatagc aagaacgtgg ctaagttgca gaagcacttc aagaaaatgt
     7681 tcgccggcgt ttccagcatc atcttaaatg aggataactc tgtggtcctc ggcatctcat
     7741 cccgagaagg ggaggaggtt atgtttaaaa ctccagtgtc catcactgaa caccccaaaa
     7801 tcaatgagtg gctcactctg gtggaaaagg agatgagagt caccttggct aaacttctgg
     7861 cagagtctgt tacagaagtg gagatttttg gaaaagcaac ttctattgac ccaaatacct
     7921 acattacttg gattgataaa taccaggccc agctagtggt attgtcagcc cagatagcct
     7981 ggtccgagaa cgtggagaat gcgctgagca acgttggggg aggtggtgat gtggggccct
     8041 tgcagtctgt gctgagcaat gtggaggtca cactcaacgt cttagcggac tctgtcctca
     8101 tggagcagcc tcctctccgg agacggaagc tggagcactt gatcacagag ctggttcacc
     8161 aaagagacgt taccaggtcc ttgatcaaaa gcaagatcga caacgccaaa tctttcgaat
     8221 ggctcagcca gatgcgattt tactttgacc ctaagcaaac ggacgtgtta cagcagctgt
     8281 ccattcagat ggccaatgcc aagtttaact acggctttga gtacctgggt gttcaagaca
     8341 agctggttca gacgcccctc actgaccgtt gctatttgac aatgacacaa gctttggagg
     8401 ccaggcttgg gggctcccca tttggacctg ctggaaccgg aaagacagag tccgtcaaag
     8461 ccctcggcca tcagctgggg cggttcgtct tagttttcaa ctgtgacgaa acctttgatt
     8521 tccaggcgat gggacgtatc tttgtggggc tttgccaagt gggcgcgtgg ggctgctttg
     8581 atgagtttaa ccgcctggag gagcggatgc tctcagctgt atcccagcag gtgcagtgca
     8641 tacaagaggc actgcgggag cattccaacc ccaactacga caagacctca gcccccatta
     8701 cttgtgagct gctgaacaaa caagtcaagg ttagcccaga catggctatc ttcatcacca
     8761 tgaacccagg ctatgcaggt cgctcaaacc tccccgacaa cctcaagaag ctcttccgga
     8821 gcttggcgat gaccaagcct gaccggcagc tgatcgccca ggtcatgctg tactcgcagg
     8881 gcttccggac tgctgaggtc cttgccaaca agatcgtccc gttcttcaaa ctgtgtgatg
     8941 agcaactgtc ttctcaaagc cattatgact ttgggcttcg ggctttgaag agtgtgcttg
     9001 taagtgcagg caatgtgaag agggagagga tccagaagat aaagagggag aaagaagaac
     9061 ggggggaagc agttgatgaa ggagagattg ctgagaacct acctgagcag gagattctga
     9121 tccagagtgt ctgcgagaca atggtgccca agctggtggc agaagatatc ccgctgctct
     9181 tcagcctcct gtctgacgtg tttcctggag tccagtacca ccgtggggag atgacagccc
     9241 ttcgagaaga gctgaagaaa gtgtgtcagg agatgtactt gacatatggc gacggagaag
     9301 aagttggcgg gatgtgggtt gaaaaggttc tccagctcta tcagatcacg cagattaacc
     9361 acggcctgat gatggttggg ccttcaggaa gtgggaagag catggcctgg cgagtgctgc
     9421 tgaaggctct agagaggctt gagggtgtgg agggcgtggc ccacataatt gaccccaagg
     9481 ccatcagcaa agaccacctc tacggaacct tggatcccaa caccagggag tggacagacg
     9541 gactcttcac acacgtgctg agaaagatca tagacaatgt gagaggtgag ctgcagaaac
     9601 gccagtggat tgtctttgat ggcgatgtgg accccgagtg ggtggagaac ctgaactccg
     9661 tgctggatga caacaagctc ctgacgctgc ctaatggaga gcgccttagc ctgcctccca
     9721 atgtgcgaat aatgtttgaa gtgcaggact tgaaatacgc gacgctggcc acggtgtctc
     9781 gctgtggcat ggtgtggttc agtgaggacg tcctgagcac agacatgatc ttcaataact
     9841 tcctggccag gctgcgcagc attcccctgg atgaagggga ggacgaggca cagcgtaggc
     9901 gaaaaggcaa agaagacgag ggcgaagagg ccgcttctcc aatgctgcag attcaaagag
     9961 atgcagcaac gatcatgcag ccgtacttca cgtccaacgg cctggtcacc aaggcgctgg
    10021 agcacgcctt caagctggag cacatcatgg acctgacgcg cctgcgctgc ctgggctcgc
    10081 tcttctccat gctgcaccag gcctgccgca acgtggcgca gtacaatgcc aaccatcctg
    10141 atttccccat gcagattgag cagctggagc gctacattca gcgctatctg gtttacgcca
    10201 tcctctggtc cttatctgga gacagccggc tgaagatgag agcagagctg ggcgagtaca
    10261 tccggaggat caccactgtg ccgctgccca ctgcgcccaa cgtccccatc attgactatg
    10321 aggtgtccat cagtggagag tggtcgccgt ggcaggccaa ggtgcctcag attgaagtgg
    10381 agacacacaa ggtggcagcc ccagatgtcg tcgtgccaac cttagataca gtccgccatg
    10441 aagctctcct gtacacttgg ctggctgaac acaaacccct ggtcttatgt ggccctcctg
    10501 ggtctggcac gaccatgaca ctcttcagtg ctctccgggc cttgcctgac atggaggttg
    10561 tgggcctaaa cttctcaagt gccactacac cagagctgtt gctgaagacg tttgaccact
    10621 actgcgaata caggcgtaca cctaacggtg ttgtcttggc tcctgttcag cttggaaagt
    10681 ggctggtctt gttctgtgat gaaatcaact tgcccgatat ggataaatac ggcacacaga
    10741 gagtcatctc cttcatcaga cagatggtgg agcatggagg cttctatcgt acctcagacc
    10801 aaacgtgggt aaagctagag agaatccagt ttgttggggc ttgcaatcca cccactgacc
    10861 ctgggagaaa gcccctctca cacaggttct tgcgccacgt gcctgttgta tatgtggatt
    10921 acccaggacc agcctccctg acgcaaatct atggcacttt taaccgcgcc atgctgaggc
    10981 tcattccctc gctccgtacc tatgcagaac ccctcactgc cgccatggtg gagttctata
    11041 ccatgtcaca ggagcggttt actcaggaca cacagccaca ctatatctac tcgccccgtg
    11101 agatgactcg gtgggtaaga ggaatctttg aagcactgag acccctggag acattgcctg
    11161 ttgaaggtct cattcgtatt tgggcacatg aagccctccg tctcttccaa gacaggcttg
    11221 tggaggatga ggagcggcgc tggactgacg aaaacattga catggttgcc ttgaagcact
    11281 tccctaacat agacaaagag aaagccatga gccggcctat cctgtacagc aactggctgt
    11341 caaaggatta tatcccagtt gaccaagaag agctaaggga ttatgtcaag gctaggctga
    11401 aggtcttcta tgaagaagaa cttgatgttc ccttggtctt attcaatgaa gttctggacc
    11461 atgtgctgag gatcgacaga atattccgac aacctcaagg ccatctgctt ctgattggtg
    11521 ttagtggagc tggaaaaacc accctgtctc gctttgtcgc ctggatgaat ggcttgagtg
    11581 tataccagat taaggtccac aggaagtaca ctggggaaga cttcgatgaa gatcttagga
    11641 cagtgctgag acggtctggc tgtaaaaatg aaaagatagc attcataatg gatgagtcca
    11701 acgtcttaga ttccggattc ctggaacgaa tgaataccct tctggctaat ggcgaggttc
    11761 ctgggctctt tgaaggggat gagtatgcca cgctgatgac acagtgcaaa gagggggccc
    11821 agaaggaggg gctcatgctg gactcccacg aggagctcta caagtggttc accagccagg
    11881 tgatccgcaa cctgcacgtg gtcttcacca tgaacccgtc ctcagagggg ctcaaggaca
    11941 gggcggccac ctcaccagcc cttttcaaca ggtgtgtgct gaactggttt ggtgactggt
    12001 ccactgaagc actgtatcaa gtcggcaaag aattcaccag taagatggac ctggagaagc
    12061 cgaactatat agtgcctgac tacatgccag ttgtgtatga taaactgccg cagcccccaa
    12121 cacaccggga agccattgtg aacagctgtg tgtttgtcca tcagactctt caccaggcta
    12181 atgcgcgcct ggcgaagcga ggtggcagaa cgatggccat cacacctcgg cactacctgg
    12241 acttcatcaa ccactacgcc aacctcttcc atgagaagcg cagcgagttg gaggagcagc
    12301 agatgcacct gaatgttggg ctccgaaaaa tcaaagagac cgtggaccag gtggaagaac
    12361 tgcgccgtga cctgaggatc aagagccaag agctggaggt caagaacgca gcggccaatg
    12421 acaagctgaa gaaaatggtg aaagaccagc aagaggctga gaagaagaag gtcatgagcc
    12481 aagaaatcca ggagcagcta cacaaacagc aggaagtgat cgcagacaag cagatgagtg
    12541 tcaaggagga cctggataaa gtggagcctg ctgtcatcga ggcccagaac gctgtgaagt
    12601 ccatcaagaa gcagcacctg gtggaggtga ggtccatggc caaccctcct gcagctgtga
    12661 agctggctct ggagtccatc tgcctgctgc ttggtgagag caccacggac tggaagcaga
    12721 tccgctccat catcatgaga gagaacttca tccccaccat cgtcaacttc tcggctgagg
    12781 agatcagtga tgccatacga gagaagatga agaaaaacta catgtccaac cccagttaca
    12841 actatgagat cgtcaaccgg gcttccctgg cttgtggtcc tatggtgaag tgggcgattg
    12901 cacagctcaa ttatgcagac atgttaaagc gagtggagcc cctgaggaat gagctgcaga
    12961 agctggaaga tgacgccaag gacaaccagc agaaagccaa tgaggtggag cagatgatca
    13021 gggacctgga agccagcatt gcccgctaca aggaggagta cgctgtcctc atctctgagg
    13081 cccaggccat caaggcagac ctggcagctg tggaagcaaa ggtaaaccgg agcactgcac
    13141 ttctaaagag tttatctgcc gaaagggaac gttgggaaaa gacgagtgaa actttcaaaa
    13201 accagatgtc taccattgct ggggactgcc tcctgtcggc ggccttcatt gcttacgctg
    13261 gctactttga ccagcaaatg cgccagaact tgttcaccac ctggtctcat cacttacagc
    13321 aagccaacat ccagttccgc acagacattg caaggacgga gtacctctcc aatgctgacg
    13381 aacgcctccg ctggcaggcc agctctctgc ctgccgacga cctgtgcaca gagaatgcca
    13441 tcatgctaaa acgattcaat aggtacccgc tgatcatcga cccctccgga caggccacag
    13501 aattcatcat gaatgaatat aaggaccgga agatcacaag gaccagcttc ttagatgatg
    13561 ccttccggaa gaatttggag agtgctctga gatttggcaa tccccttctt gtccaggatg
    13621 tggaaagcta cgatccagtt ttgaatccag tactgaaccg tgaagttcgg cgaacaggag
    13681 ggagggtgct gattaccctc ggtgaccaag acatagacct gtcaccatcc tttgtcatct
    13741 ttctgtctac ccgagaccca actgtggagt tcccaccaga cctctgctcc cgggtgacct
    13801 ttgtgaactt caccgtcacc cgcagcagtt tacagagcca gtgtctcaat gaagtgctta
    13861 aagcagaaag gccagacgtg gacgagaagc gctccgacct cctgaaactg caaggggagt
    13921 tccaactccg tttacggcag cttgaaaagt ctctgctcca agctctgaat gaggtaaaag
    13981 gccgcattct ggatgacgac accatcatta caaccctgga gaatctgaag cgggaagctg
    14041 ccgaggttac cagaaaggtg gaggagacag atattgtcat gcaggaggtg gagaccgtgt
    14101 cccagcagta cctgccgctg tccactgcct gcagcagcat ctacttcact atggagtcct
    14161 tgaagcaggt tcacttcctg taccagtact ctctgcagtt cttcctggac atctaccaca
    14221 acgtgctgta tgagaacccc aacctgaagg gtgccaccga tcacacacag cgcctctcca
    14281 tcataaccaa ggacctcttc caggtggcct ttaaccgagt ggctcgaggc atgctgcacc
    14341 aggaccacat caccttcgcc atgctgctag cgaggatcaa gctgaaaggc accgtggggg
    14401 agcccaccta tgatgcagag ttccagcact tcctcagagg gaaggagatt gtgctgagtg
    14461 ctggctccac ccccaaaatc cagggcctga ccgtagagca ggcagaagct gtcgtgaggt
    14521 tgagctgtct gcctgcattt aaagatctga ttgccaaggt gcaggcagat gagcagtttg
    14581 gcatttggct ggatagcagt tcaccagagc agaccgtgcc atacctctgg agcgaggaaa
    14641 cgcctacaac tcccattggg caggccatcc accgcctgct cctgattcaa gccttccgac
    14701 ccgaccgcct gttggccatg gcccacatgt ttgtttccac aaacctcgga gagtccttca
    14761 tgtccatcat ggagcagcct ctcgacctca cccacattgt gggcacagag gtgaagccaa
    14821 acacacctgt cctgatgtgc tctgtgcctg ggtatgatgc cagcggacat gttgaggacc
    14881 tcgcagcaga gcagaacacg cagatcacgt ctattgccat cggctcagca gaaggcttta
    14941 accaagcaga taaggccatc aacaccgccg tgaagtcagg caggtgggtg atgttgaaga
    15001 acgtgcacct ggccccgggc tggctgatgc agctggagaa gaagctgcac tccctgcagc
    15061 cccacgcctg cttccgactt ttcctcacca tggagatcaa ccccaaggtg cccgtgaacc
    15121 tgctccgtgc tggtcggatc tttgtgtttg agccaccacc cggagtgaaa gccaacatgc
    15181 tgaggacatt tagcagcatc cctgtctcta ggatatgcaa gtctccaaat gagcgcgccc
    15241 gcttgtattt cctgctggct tggttccatg ccatcatcca agagcgcctg cgctatgctc
    15301 cactggggtg gtcaaagaag tacgaatttg gagaatctga ccttcgatca gcttgtgaca
    15361 cggtggacac gtggctggac gacacagcca agggccggca gaacatctca ccggataaga
    15421 tcccatggtc tgccctgaag accctgatgg cgcagtccat ctatggtggg cgggtggaca
    15481 atgagttcga ccagcgtctg ctcaacacct tcctggagcg gctgttcacc acccgcagct
    15541 tcgacagtga attcaagctg gcgtgcaagg ttgacgggca caaggacatc cagatgcctg
    15601 atggtatcag gcgagaggag tttgtgcagt gggtggagct gctgcctgac gcccagacac
    15661 cctcatggct gggcctcccc aacaatgctg aaagggtcct gcttaccacc caaggcgtgg
    15721 acatgatcag caagatgctg aagatgcaga tgctggagga cgaggatgac ctggcctacg
    15781 cagagacgga gaagaaggca aggacggact ccacctccga tggccgtcca gcctggatga
    15841 ggacactgca caccacggcg tccaactggc tgcacctcat cccacagaca ctgagccccc
    15901 tgaagcgcac cgtggagaac atcaaggatc ctttgtttag gttctttgag agagaagtaa
    15961 agatgggggc caagttactc caagatgtgc gccaagatct tgctgacgtc gttcaagtgt
    16021 gcgaggggaa aaagaagcag acaaactacc tgcgcacgct catcaatgag ctggtgaaag
    16081 ggatcttgcc tcggagctgg tcccattaca cagtgcctgc aggcatgact gtgatccagt
    16141 gggtgtcaga tttcagcgag aggatcaaac agctgcagaa catctcacaa gcagctgcat
    16201 ctggtggtgc caaggaactg aagaacatcc acgtgtgcct gggtggcctg tttgtacctg
    16261 aggcctacat cactgccacc agacagtatg tggcacaagc taacagctgg tccctggagg
    16321 aactctgcct ggaagtcaat gtcaccgcct cacagagtgc cacccttgat gcctgcagct
    16381 ttggagtcac aggtctgaag ctccaagggg ccacgtgcag caacaacaag ctatcactct
    16441 ccaatgccat ctccacagtc ctccccctca cacagctgcg ctgggtgaaa caaacaagcg
    16501 ccgagaagaa ggccagcgtg gtgaccttac ctgtctacct gaacttcacc cgggcagacc
    16561 tcatcttcac cgtggacttt gaaattgcta caaaggaaga tcctcgcagc ttctatgagc
    16621 gtggagttgc cgttctgtgc actgagtaat taatctagaa gcttcgaatt ctgcagtcg
//
