LOCUS       sequence-37096-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Werdien D, Peiler G, Ryffel GU
  TITLE     FLP and Cre recombinase function in Xenopus embryos.
  JOURNAL   Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3.
  PUBMED    11376165
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2001 Jun 1. 29(11):E53-3."
COMMENT     Sequence Label: LCMV:GFP(FRT)DsRed
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      166..175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(200..233)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    235..369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(259..278)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     313..332
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(534..554)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gnagcagggg gccgcccttg gtcaccttca gcttcacggt gttgtggccc tcgtaggggc
       61 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggtgccc tccatgcgca
      121 ccttgaagcg catgaactcc ttgatgacgt tcttggagga gcgcaccatg gtggcgaccg
      181 gtggatctcc taggcccggg aagttcctat actttctaga gaataggaac ttcggatcca
      241 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
      301 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      361 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
      421 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta
      481 gaggctcgag ttatttgtat agttcatcca tgccatgtgt aatcccagca gccgttacaa
      541 actcaagaag gaccatgtgg tctctctttt cgttgggatc tttcgaaagg gcagattgtg
      601 tggacaggta atggttgtct ggtaaaagga cagggtcatc gccaattgga gtattttgtt
      661 gataatgatc agcgagttgc acgccgccgt cttcgatgtt gtggcgggtc ttgaagttgg
      721 ctttgatgcc gttcttctgc ttgtcggcca tgatgtatac gttgtgggag ttgtagttgt
      781 attccaactt gtggccgagg atgtttccgt cctccttgaa atcgattccc ttaagctcga
      841 tcctgttgac gagggtgtct ccctcaaac
//
