LOCUS       sequence-370972-        9319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY 
            chain that is within the UTR of eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9319)
  AUTHORS   Hughes NW, Qu Y, Zhang J, Tang W, Pierce J, Wang C, Agrawal A, Morri
            M, Neff N, Winslow MM, Wang M, Cong L
  TITLE     Machine-learning-optimized Cas12a barcoding enables the recovery of 
            single-cell lineages and transcriptional profiles.
  JOURNAL   Mol Cell. 2022 Jun 22. pii: S1097-2765(22)00537-8. doi: 
            10.1016/j.molcel.2022.06.001.
  PUBMED    35752172
REFERENCE   2  (bases 1 to 9319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 Jun 22. pii: S1097-2765(22)00537-8. doi: 
            10.1016/j.molcel.2022.06.001."
COMMENT     Sequence Label: pN256
FEATURES             Location/Qualifiers
     source          1..9319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(53..72)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        244..623
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        625..823
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             841..1021
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1068..1193
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     promoter        complement(1728..1976)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(1786..1805)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(1956..1976)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2127..2360
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2545..2589
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2887..3004
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    3054..3102
                     /note="synthetic polyadenylation signal"
     misc_feature    3256..3274
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_feature    3308..3896
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3414..3425
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3823..3843
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(3924..4637,4638..4640,4641..4643))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLTTQSALSKDPNEKRDHMVLV
                     EFVTAAGITLGMDELYK"
     primer_bind     4318..4337
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4577..4598
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4640..4649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4656..5005)
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     primer_bind     complement(4693..4717)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4763..4781
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4799..4817
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4835..4853
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4871..4889
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4907..4925
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4943..4961
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4979..4997
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     LTR             5077..5310
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5336..5353)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5342..5566
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5612..6040
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5699..5718)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5909..5930
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6049..6069)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6054..6383
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6234..6369
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6296..6315
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6431..6478
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6497..6871
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7001..7122
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7038..7057)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7092..7111
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7171..7187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7171..7187)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7184..7206)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7195..7211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7219..7225,7226..7243,7244..7249))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7264..7285
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7402..7419)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7573..8161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7653..7672)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8332..9123,9124..9192))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8955..8974
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9193..9297)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
       61 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
      121 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
      181 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatataccc
      241 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
      301 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      361 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      421 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      481 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      541 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      601 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      661 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      721 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      781 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
      841 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      901 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      961 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     1021 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     1081 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     1141 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     1201 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     1261 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     1321 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     1381 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     1441 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     1501 aaaacaaaag taagaccacc gcacagcaag cggccgcaaa aaatctacga cagtagaaat
     1561 taaattgtcc tagtaatacg atgatctccg agaggagaaa ttcaggaagt ctctcgatgt
     1621 gccgatctac gacagtagaa attaacttgt cctagtaata ggtagatcta ctagagtaga
     1681 aatttagaat gtctcggaat gtgccgatct acaagagtag aaattacggt gtttcgtcct
     1741 ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt aagcatatga
     1801 tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta ttactttcta
     1861 cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattct aattatctct
     1921 ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag gccctcgctg
     1981 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     2041 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     2101 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     2161 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     2221 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     2281 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     2341 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     2401 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     2461 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     2521 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     2581 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     2641 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     2701 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     2761 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     2821 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
     2881 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2941 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     3001 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaagg taccacacaa
     3061 aaaaccaaca cacagatcta atgaaaataa agatctttta ttatagttac gccaagcttg
     3121 aattcggcgc acgattttgc tacctattac taggacaagt ggaggcgttg agaatgtctc
     3181 ggaatgtgcc ggaaatttgc atcgtattac taggacaatt cgagtccttg aggaagtctc
     3241 tcgatgtgcc ggaaactgtc tcttatacac atctccgagc ccacgagaca cgcgtcatgg
     3301 attcgaggcg gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga
     3361 cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg
     3421 gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc
     3481 agccaaggaa aggacgatga tttccccgac aacaccacgg aattgtcagt gcccaacagc
     3541 cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg
     3601 agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg
     3661 ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac
     3721 tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg
     3781 gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca
     3841 acatagttaa gaataccagt caatctttca caaattttgt aatccagagg ttgattgtcg
     3901 acttaacgcg atctatgtct agattacttg tacagctcgt ccatgccgag agtgatcccg
     3961 gcggcggtca cgaactccac caggaccatg tgatcgcgct tctcgttggg gtctttgctc
     4021 agggcggact gggtggtcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
     4081 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
     4141 atcttgaagt tcgccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
     4201 ctgttgaagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
     4261 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
     4321 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
     4381 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
     4441 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
     4501 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
     4561 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
     4621 agctcctcgc ccttgctcac catggtggcg ctagcaagta taagacaaaa gtgttgtgga
     4681 attgctccag gcgatctgac ggttcactaa acgagctctg cttttatagg cgcccaccgt
     4741 acacgcctac ctcgacatac gttctctatc actgataggg agtaaactgg atatacgttc
     4801 tctatcactg atagggagta aactgtagat acgttctcta tcactgatag ggagtaaact
     4861 ggtcatacgt tctctatcac tgatagggag taaactcctt atacgttctc tatcactgat
     4921 agggagtaaa gtctgcatac gttctctatc actgataggg agtaaactct tcatacgttc
     4981 tctatcactg atagggagta aactcctcga gtttaagacc aatgacttac aaggcagctg
     5041 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5101 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     5161 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5221 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5281 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     5341 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5401 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5461 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5521 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5581 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     5641 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5701 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5761 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5821 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5881 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5941 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6001 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     6061 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6121 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6181 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6241 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6301 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     6361 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     6421 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     6481 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     6541 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     6601 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     6661 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     6721 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     6781 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     6841 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     6901 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     6961 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     7021 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7081 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     7141 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7201 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7261 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7321 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7381 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7441 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7501 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7561 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7621 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7681 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7741 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7801 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7861 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7921 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7981 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8041 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8101 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8161 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8221 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8281 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8341 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8401 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8461 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8521 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8581 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8641 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8701 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8761 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8821 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8881 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8941 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9001 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9061 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9121 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9181 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9241 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9301 atttccccga aaagtgcca
//
