LOCUS       sequence-371043-       10677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector - tBFP reporter for Pax6-based SNIPRs with a 
            constitutive mCitrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Zhu I, Liu R, Garcia JM, Hyrenius-Wittsten A, Piraner DI, Alavi J, 
            Israni DV, Liu B, Khalil AS, Roybal KT
  TITLE     Modular design of synthetic receptors for programmed gene regulation
            in cell therapies.
  JOURNAL   Cell. 2022 Apr 14;185(8):1431-1443.e16. doi: 
            10.1016/j.cell.2022.03.023.
  PUBMED    35427499
REFERENCE   2  (bases 1 to 10677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03"; journalName: "Cell"; date: "2022-04-14- 
            14"; volume: "185"; issue: "8"; pages: "1431-1443.e16"
COMMENT     Sequence Label: pHR_Pax6-RE_tBFP_PGK_mCitrine
FEATURES             Location/Qualifiers
     source          1..10677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             103..336
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(361..379)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(362..379)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(669..688)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(902..921)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1021..1043
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1081..1099)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1167..1271
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1272..1340,1341..2132)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1490..1509)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2303..2891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2792..2811
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3045..3062
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3132..3152)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3137..3466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3317..3452
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4600..4665
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4795..4815
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5240..5374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5277..5296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5331..5350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5542..6175
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6222..6347
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6844..7077
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7262..7306
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7591..7708
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             8030..8731
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        8806..9305
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8841..8864)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9238..9257
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(9326..9328,9329..9331,9332..10045)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9371..9392)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9632..9651)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9979..10000
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    10082..10670
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10135..10155)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10553..10564)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(join(10673..10677,1..12))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(10674..10677,1..13))
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ccgtcgacct cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg
       61 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
      121 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
      181 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      241 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      301 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat
      361 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
      421 gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac
      481 cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt
      541 ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc
      601 tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
      661 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
      721 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
      781 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
      841 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca
      901 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
      961 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     1021 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     1081 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     1141 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1201 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1261 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1321 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1381 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1441 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1501 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1561 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1621 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1681 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1741 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1801 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1861 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1921 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1981 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     2041 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     2101 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     2161 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2221 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2281 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2341 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2401 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2461 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2521 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2581 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2641 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2701 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2761 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2821 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2881 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2941 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3001 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3061 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     3121 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     3301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3421 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     3481 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     3541 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     3601 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     3661 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     3721 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     3781 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     3841 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     3901 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     3961 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     4021 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     4081 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     4141 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     4201 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     4261 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     4321 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     4381 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     4441 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     4501 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
     4561 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     4621 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     4681 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     4741 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     4801 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     4861 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     4921 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
     4981 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
     5041 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
     5101 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     5161 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     5221 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     5281 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5341 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     5401 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     5461 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     5521 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     5581 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     5641 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     5701 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     5761 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     5821 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     5881 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     5941 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     6001 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6061 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6121 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     6181 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     6241 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     6301 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     6361 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     6421 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     6481 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     6541 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     6601 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     6661 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     6721 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     6781 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     6841 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     6901 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     6961 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     7021 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     7081 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     7141 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     7201 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     7261 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     7321 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     7381 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     7441 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     7501 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     7561 gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     7621 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     7681 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     7741 ttggatcgat aagcttgata tcgaattcgg gtttcgtaac aatcgcatga ggattcgcaa
     7801 cgccttattt tcacgcatga gtgcacagtc ccgtctcagt aaaggtattt tcacgcatga
     7861 gtgcacagaa tcggactgcc ttcgtaattt tcacgcatga gtgcacaggt atcagtcgcc
     7921 tcggaaattt tcacgcatga gtgcacagaa cgactagtct agagggtata taatgggggc
     7981 cactagtcta ctaccagagc tcatcgctag cgctaccgga tccgccacca tgagcgagct
     8041 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
     8101 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     8161 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
     8221 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt
     8281 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     8341 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     8401 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac
     8461 cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct
     8521 cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc
     8581 taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga
     8641 ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct
     8701 ccctagcaaa ctggggcaca agcttaatta agatccttga cttgcggccg caactcccac
     8761 ctgcaacatg cgtgactgac tgaggccgcg actctagagt cgaccgggta ggggaggcgc
     8821 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     8881 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     8941 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     9001 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     9061 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     9121 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     9181 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     9241 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     9301 tttcgacctg cagcccaagc ttaccatggt gagcaagggc gaggagctgt tcaccggggt
     9361 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     9421 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     9481 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgatgtgctt
     9541 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     9601 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     9661 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     9721 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     9781 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     9841 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     9901 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc
     9961 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
    10021 cggcatggac gagctgtaca agtaactgca ggcatgcaag cttgatatca agcttatcga
    10081 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
    10141 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
    10201 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
    10261 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
    10321 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
    10381 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
    10441 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
    10501 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
    10561 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
    10621 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgata
//
