LOCUS       sequence-371048-       10572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector - tBFP reporter for HNF1A-based SNIPRs with a 
            constitutive mCitrine;.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10572)
  AUTHORS   Zhu I, Liu R, Garcia JM, Hyrenius-Wittsten A, Piraner DI, Alavi J, 
            Israni DV, Liu B, Khalil AS, Roybal KT
  TITLE     Modular design of synthetic receptors for programmed gene regulation
            in cell therapies.
  JOURNAL   Cell. 2022 Apr 14;185(8):1431-1443.e16. doi: 
            10.1016/j.cell.2022.03.023.
  PUBMED    35427499
REFERENCE   2  (bases 1 to 10572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03"; journalName: "Cell"; date: "2022-04-14- 
            14"; volume: "185"; issue: "8"; pages: "1431-1443.e16"
COMMENT     Sequence Label: pHR_HNF1A-RE_tBFP_PGK_mCitrine
FEATURES             Location/Qualifiers
     source          1..10572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             93..326
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(351..369)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(352..369)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(659..678)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(892..911)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1011..1033
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1071..1089)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1157..1261
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1262..1330,1331..2122)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1480..1499)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2293..2881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2782..2801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3035..3052
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3122..3142)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3127..3456
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3307..3442
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4590..4655
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4785..4805
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5230..5364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5267..5286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5321..5340
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5532..6165
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6212..6337
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6834..7067
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7252..7296
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7581..7698
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             7915..8616
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        8691..9190
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8726..8749)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9123..9142
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(9211..9213,9214..9216,9217..9930)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9256..9277)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9517..9536)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9864..9885
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9967..10555
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10020..10040)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10438..10449)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(join(10558..10572,1..2))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(10559..10572,1..3))
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
       61 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
      121 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
      181 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      241 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      301 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
      361 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
      421 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
      481 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
      541 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
      601 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
      661 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
      721 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
      781 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
      841 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
      901 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
      961 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     1021 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     1081 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     1141 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1201 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1261 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1321 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1381 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1441 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1501 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1561 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1621 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1681 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1741 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1801 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1861 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1921 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1981 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2041 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2101 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2161 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2221 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2281 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2341 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2401 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2461 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2521 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2581 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2641 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2701 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2761 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2821 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2881 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2941 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3001 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3061 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     3121 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3181 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     3241 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3301 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3361 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     3421 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg
     3481 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     3541 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     3601 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     3661 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     3721 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg
     3781 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
     3841 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
     3901 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
     3961 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
     4021 gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc
     4081 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
     4141 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
     4201 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
     4261 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
     4321 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
     4381 cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga
     4441 acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta
     4501 tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     4561 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     4621 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     4681 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
     4741 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
     4801 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
     4861 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
     4921 tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat aacagttata
     4981 atcataacat actgtttttt cttactccac acaggcatag agtgtctgct attaataact
     5041 atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt
     5101 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
     5161 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     5221 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5281 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5341 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     5401 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     5461 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     5521 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     5581 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     5641 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     5701 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     5761 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     5821 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     5881 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     5941 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     6001 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6061 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6121 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     6181 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     6241 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     6301 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     6361 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     6421 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     6481 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     6541 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     6601 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     6661 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     6721 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     6781 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     6841 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     6901 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     6961 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     7021 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     7081 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     7141 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     7201 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     7261 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     7321 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     7381 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     7441 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     7501 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa
     7561 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     7621 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     7681 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggatcgat
     7741 aagcttgata tcgaattcat cgatgttaat aattaacata tatgttaatc attaacatat
     7801 agttaattat taaccgctat gttaatgatt aacaacgact agtctagagg gtatataatg
     7861 ggggccacta gtctactacc agagctcatc gctagcgcta ccggatccgc caccatgagc
     7921 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
     7981 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
     8041 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
     8101 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
     8161 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
     8221 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
     8281 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     8341 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
     8401 aagctcgtgg gcgggagcca tctgatcgca aacatcaaga ccacatatag atccaagaaa
     8461 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
     8521 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
     8581 gacctcccta gcaaactggg gcacaagctt aattaagatc cttgacttgc ggccgcaact
     8641 cccacctgca acatgcgtga ctgactgagg ccgcgactct agagtcgacc gggtagggga
     8701 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     8761 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     8821 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     8881 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     8941 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     9001 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     9061 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     9121 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     9181 gggcctttcg acctgcagcc caagcttacc atggtgagca agggcgagga gctgttcacc
     9241 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     9301 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     9361 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg
     9421 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     9481 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     9541 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     9601 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     9661 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     9721 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     9781 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa
     9841 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     9901 actctcggca tggacgagct gtacaagtaa ctgcaggcat gcaagcttga tatcaagctt
     9961 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
    10021 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
    10081 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
    10141 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
    10201 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
    10261 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
    10321 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
    10381 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    10441 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    10501 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
    10561 taccgtcgac ct
//
