LOCUS       sequence-371049-       10642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector - tBFP reporter for Gal4-based SNIPRs with a 
            constitutive mCitrine;.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10642)
  AUTHORS   Zhu I, Liu R, Garcia JM, Hyrenius-Wittsten A, Piraner DI, Alavi J, 
            Israni DV, Liu B, Khalil AS, Roybal KT
  TITLE     Modular design of synthetic receptors for programmed gene regulation
            in cell therapies.
  JOURNAL   Cell. 2022 Apr 14;185(8):1431-1443.e16. doi: 
            10.1016/j.cell.2022.03.023.
  PUBMED    35427499
REFERENCE   2  (bases 1 to 10642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03"; journalName: "Cell"; date: "2022-04-14- 
            14"; volume: "185"; issue: "8"; pages: "1431-1443.e16"
COMMENT     Sequence Label: pHR_Gal4UAS_tBFP_PGK_mCitrine
FEATURES             Location/Qualifiers
     source          1..10642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             79..312
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(337..355)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(338..355)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(645..664)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(878..897)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     997..1019
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1057..1075)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1143..1247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1248..1316,1317..2108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1466..1485)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2279..2867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2768..2787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3021..3038
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3108..3128)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3113..3442
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3293..3428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4576..4641
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4771..4791
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5216..5350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5253..5272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5307..5326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5518..6151
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6198..6323
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6820..7053
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7238..7282
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7567..7684
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             7947..8648
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        8723..9222
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8758..8781)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9155..9174
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(9267..9269,9270..9272,9273..9986)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9312..9333)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9573..9592)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9920..9941
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    10023..10611
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10076..10096)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10494..10505)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10614..10630)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10615..10631)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
       61 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
      121 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
      181 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
      241 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
      301 aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat
      361 gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa
      421 gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc
      481 cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc
      541 ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg
      601 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
      661 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
      721 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
      781 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
      841 gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat
      901 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      961 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     1021 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     1081 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     1141 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1201 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1261 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1321 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1381 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     1441 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     1501 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1561 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1621 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1681 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1741 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1801 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1861 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1921 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1981 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2041 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2101 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2161 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2221 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2281 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2341 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2401 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2461 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2521 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2581 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2641 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2701 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2761 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2821 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2881 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2941 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3001 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3061 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
     3121 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3181 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3241 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3301 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3361 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3421 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
     3481 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
     3541 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
     3601 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
     3661 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
     3721 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
     3781 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
     3841 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
     3901 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
     3961 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
     4021 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
     4081 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
     4141 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
     4201 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
     4261 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
     4321 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
     4381 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
     4441 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
     4501 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     4561 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     4621 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     4681 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     4741 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     4801 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     4861 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     4921 atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca taacatactg
     4981 ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg
     5041 tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc
     5101 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     5161 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     5221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5341 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     5401 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     5461 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     5521 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     5581 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     5641 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     5701 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     5761 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     5821 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     5881 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     5941 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     6001 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6061 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6121 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     6181 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     6241 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     6301 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     6361 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     6421 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     6481 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     6541 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     6601 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     6661 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     6721 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     6781 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     6841 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     6901 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     6961 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     7021 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     7081 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     7141 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     7201 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     7261 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     7321 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     7381 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     7441 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     7501 agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat
     7561 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     7621 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     7681 ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc ttgatatcga
     7741 attcggagca ctgtcctccg aacgtcggag cactgtcctc cgaacgtcgg agcactgtcc
     7801 tccgaacgtc ggagcactgt cctccgaacg gagcatgtcc tccgaacgtc ggagcactgt
     7861 cctccgaacg actagtctag agggtatata atgggggcca ctagtctact accagagctc
     7921 atcgctagcg ctaccggatc gccaccatga gcgagctgat taaggagaac atgcacatga
     7981 agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag
     8041 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc
     8101 ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc
     8161 acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga
     8221 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
     8281 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg
     8341 tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac cccgctgacg
     8401 gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg
     8461 caaacatcaa gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg
     8521 tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag acctacgtcg
     8581 agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc
     8641 ttaattaaga tccttgactt gcggccgcaa ctcccacctg caacatgcgt gactgactga
     8701 ggccgcgact ctagagtcga ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     8761 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     8821 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     8881 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     8941 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     9001 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     9061 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     9121 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     9181 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgaattctca cgcgtcaagt
     9241 ggagcaaggc aggtggacag tggatcatgg tgagcaaggg cgaggagctg ttcaccgggg
     9301 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     9361 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     9421 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgatgtgct
     9481 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     9541 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     9601 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     9661 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     9721 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     9781 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     9841 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg agcaaagacc
     9901 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     9961 tcggcatgga cgagctgtac aagtaagatc cggcatgcaa gcttgatatc aagcttatcg
    10021 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
    10081 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
    10141 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
    10201 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
    10261 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
    10321 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
    10381 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
    10441 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
    10501 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    10561 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
    10621 gtcgacctcg agggaattaa tt
//
