LOCUS       sequence-371062-        4335 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of cAMP sensor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4335)
  TITLE     Expression of cAMP sensor
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Epac-SH226
FEATURES             Location/Qualifiers
     source          1..4335
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..519)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2905..4335
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactactt tagcgacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgcccgatat cagcccgtgg gaactcatga gatggaggag
      721 gagttggccg aagctgtggc cctgctctcc cagcgggggc ctgacgccct gctcactgtg
      781 gcacttcgaa agcccccagg tcagcgcacg gatgaagagc tggacctcat ctttgaggag
      841 ctgctgcaca tcaaggctgt ggcccacctc tccaactcgg tgaagcgaga attagcggct
      901 gttctgctct ttgaaccaca cagcaaggca gggaccgtgt tgttcagcca gggggacaag
      961 ggcacttcgt ggtacattat ctggaaggga tctgtcaacg tggtgaccca tggcaagggg
     1021 ctggtgacca ccctgcatga gggagatgat tttggacagc tggctctggt gaatgatgca
     1081 ccccgggcag ccaccatcat cctgcgagaa gacaactgtc atttcctgcg tgtggacaag
     1141 caggacttca accgtatcat caaggatgtg gaggcaaaga ccatgcggct ggaagaacat
     1201 ggcaaagtgg tgctggtgct ggagagagcc tctcagggcg ccggcccttc ccgaccccca
     1261 accccaggca ggaaccggta tacagtgatg tctggcaccc cagagaagat cctagagctt
     1321 ctgttggagg ccatgggacc agattccagt gctcatgacc caacagagac attcctcagc
     1381 gacttcctcc tgacccacag ggtcttcatg cccagcgccc aactctgcgc tgcccttctg
     1441 caccacttcc atgtggagcc tgcgggtggc agcgagcagg agcgcagcac ctacgtctgc
     1501 aacaagaggc agcagatctt gcggctggtc agccagtggg tggccctgta tggctccatg
     1561 ctccacactg accctgtggc caccagcttc ctccagaaac tctcagacct ggtgggcagg
     1621 gacacccgac tcagcaacct gctgagggag cagtggccag agaggcggcg atgccacagg
     1681 ttggagaatg gctgtgggaa tgcatctcct cagatgaagg cccggaactt gcctgtttgg
     1741 ctccccaacc aggacgagcc ccttcctggc agcagctgtg ccatccaagt tggggataaa
     1801 gtcccctatg acatctgccg gccagaccac tcagtgttga ccctgcagct gcctgtgaca
     1861 gcctccgtga gagaggtgat ggcagcgttg gcccaggagg atggctggac caaggggcag
     1921 gtgctggtga aggtcaattc tgcaggtgat gccattggcc tgcagccaga tgcccgtggt
     1981 gtggccacat ctctggggct caatgagcgt ctctttgttg tcaacccaca ggaagcgcat
     2041 gagctgatcc cacaccctga ccagctgggg cccactgtgg gctctgctga ggggctggac
     2101 ctggtgagtg ccaaggacct ggcaggccag ctgacggacc acgactggag cctcttcaac
     2161 agtatccacc aggtggagct gatccactat gtgctgggcc cccagcatct gcgggatgtc
     2221 accaccgcca acctggagcg cttcatgcgc cgcttcaatg agctgcagta ctgggtggcc
     2281 accgagctgt gtctctgccc cgtgcccggc ccccgggccc agctgctcag gaagttcatt
     2341 aagctggcgg cccacctcaa ggagcagaag aatctcaatt ccttctttgc cgtcatgttt
     2401 ggcctcagca actcggccat cagccgccta gcccacacct gggagcggct gccccacaaa
     2461 gtccggaagc tgtactccgc cctcgagagg ctgctggatc cctcatggaa ccaccgggta
     2521 taccgactgg ccctcgccaa gctctcccct cctgtcatcc ccttcatgcc ccttcttctc
     2581 aaagacatgg ccgccattca tgagggaaac cacacactag tggagaatct catcaacttt
     2641 gagaagatga gaatgatggc cagagccgcg cggatgctgc accactgccg aagccacaac
     2701 cctgtgcctc tctcaccact cagaagccga gtttcccacc tccacgagga cagccaggtg
     2761 gcgaggattt ccacatgctc ggagcagtcc ctgagcaccc ggagtccagc cagcacctgg
     2821 gcttatgtcc agcagctgaa ggtcattgac aaccagcggg aactctcccg cctctcccga
     2881 gagctggagc cagcaagcga gctcatggtg agcaagggcg aggaggtcat caaagagttc
     2941 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     3001 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     3061 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     3121 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     3181 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     3241 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     3301 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     3361 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     3421 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     3481 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     3541 gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc
     3601 accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa
     3661 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     3721 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     3781 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     3841 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     3901 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     3961 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     4021 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     4081 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     4141 ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     4201 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     4261 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac
     4321 gagctgtaca agtaa
//
