LOCUS       sequence-371121-       11116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PKMYT1 (human) tagged with C-terminal 
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11116)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-PKMYT1-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..11116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4885..4909)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4911..4952
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4959..5915
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(5921..5941)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    5970..6558
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6023..6043)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6441..6452)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6607..6630)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6991..7010
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7079..7678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7079..7098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7575..7595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7816..8049
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8121..8242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8158..8177)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8212..8231
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8282..8417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8437..8456)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8438..8456)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8466..8483)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8466..8482)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8475..8497)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8624..9079
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8711..8730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8921..8942
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9105..9209
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9210..9278,9279..10070)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10241..10829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10730..10749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10983..11000
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acacaagttt gtacaaaaaa gttggcacca tgctagaacg gcctcctgca ctggccatgc
     3421 ccatgcccac ggagggcacc ccgccacctc tgagtggcac ccccatccca gtcccagcct
     3481 acttccgcca cgcagaacct ggattctccc tcaagaggcc cagggggctc agccggagcc
     3541 tcccacctcc gccccctgcc aagggcagca ttcccatcag ccgcctcttc cctcctcgga
     3601 ccccaggctg gcaccagctg cagccccggc gggtgtcatt ccggggcgag gcctcagaga
     3661 ctctgcagag ccctgggtat gacccaagcc ggccagagtc cttcttccag cagagcttcc
     3721 agaggctcag ccgcctgggc catggctcct acggagaggt cttcaaggtg cgctccaagg
     3781 aggacggccg gctctatgcg gtaaagcgtt ccatgtcacc attccggggc cccaaggacc
     3841 gggcccgcaa gttggccgag gtgggcagcc acgagaaggt ggggcagcac ccatgctgcg
     3901 tgcggctgga gcaggcctgg gaggagggcg gcatcctgta cctgcagacg gagctgtgcg
     3961 ggcccagcct gcagcaacac tgtgaggcct ggggtgccag cctgcctgag gcccaggtct
     4021 ggggctacct gcgggacacg ctgcttgccc tggcccatct gcacagccag ggcctggtgc
     4081 accttgatgt caagcctgcc aacatcttcc tggggccccg gggccgctgc aagctgggtg
     4141 acttcggact gctggtggag ctgggtacag caggagctgg tgaggtccag gagggagacc
     4201 cccgctacat ggcccccgag ctgctgcagg gctcctatgg gacagcagcg gatgtgttca
     4261 gtctgggcct caccatcctg gaagtggcat gcaacatgga gctgccccac ggtggggagg
     4321 gctggcagca gctgcgccag ggctacctgc cccctgagtt cactgccggt ctgtcttccg
     4381 agctgcgttc tgtccttgtc atgatgctgg agccagaccc caagctgcgg gccacggccg
     4441 aggccctgct ggcactgcct gtgttgaggc agccgcgggc ctggggtgtg ctgtggtgca
     4501 tggcagcgga ggccctgagc cgagggtggg ccctgtggca ggccctgctt gccctgctct
     4561 gctggctctg gcatgggctg gctcaccctg ccagctggct acagcccctg ggcccgccag
     4621 ccaccccgcc tggctcacca ccctgcagtt tgctcctgga cagcagcctc tccagcaact
     4681 gggatgacga cagcctaggg ccttcactct cccctgaggc tgtcctggcc cggactgtgg
     4741 ggagcacctc caccccccgg agcaggtgca cacccaggga tgccctggac ctaagtgaca
     4801 tcaactcaga gcctcctcgg ggctccttcc cctcctttga gcctcggaac ctcctcagcc
     4861 tgtttgagga caccctagac ccaaccccaa ctttcttgta caaagtgggc ggcaagccca
     4921 tccccaaccc cctgctgggc ctggacagca ccgctagcaa agacaatact gtgcctctga
     4981 agctgatcgc tctcctggct aatggcgagt tccatagtgg cgaacagctg ggagaaaccc
     5041 tgggcatgtc cagggccgct atcaacaagc acattcagac tctgcgcgac tggggcgtgg
     5101 acgtgttcac cgtgcccgga aagggctact ctctgcccga gcctatcccg ctgctgaacg
     5161 ctaaacagat tctgggacag ctggacggcg ggagcgtggc agtcctgcct gtggtcgact
     5221 ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat gcttgcattg
     5281 cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct ccttttggag
     5341 ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca atcggcctgg
     5401 gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca gacaaggtgc
     5461 gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc atcctggtgg
     5521 agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg attaacgtgg
     5581 ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg caggaagcag
     5641 ggattaacct ggacaggaat actctggccg ctacgctgat ccgagagctg cgggcagccc
     5701 tggaactgtt cgagcaggaa ggcctggctc catatctgcc acggtgggag aagctggata
     5761 acttcatcaa tagacccgtg aagctgatca ttggggacaa agagattttc gggattagcc
     5821 gggggattga taaacaggga gccctgctgc tggaacagga cggagttatc aaaccctgga
     5881 tgggcggaga aatcagtctg cggtctgccg aaaagtaagc caactttatt atacatagtt
     5941 gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa
     6001 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6061 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6121 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6181 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6241 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6301 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6361 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6421 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6481 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     6541 tttgggccgc ctccccgcct ggaattctac cgggtagggg aggcgctttt cccaaggcag
     6601 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     6661 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     6721 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     6781 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     6841 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     6901 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggnnnnnn nggggcgggg
     6961 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     7021 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     7081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     7141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     7201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     7261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     7321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     7381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     7441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     7501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     7561 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     7621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     7681 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catctcgagg
     7741 gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7801 aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg
     7861 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7921 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7981 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8041 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     8101 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     8161 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8221 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     8281 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     8341 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8401 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     8461 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     8521 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     8581 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     8641 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     8701 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     8761 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8821 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8881 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8941 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9001 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     9061 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     9121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     9181 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     9241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     9301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     9361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     9421 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     9481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     9541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     9601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     9661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     9721 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     9781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     9841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     9901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    10021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    10081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    10141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    10261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    10321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    10381 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    10441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    10501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    10561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    10621 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    10681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    10741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    10801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    10861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    10921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    10981 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    11041 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    11101 gtgagcgcaa cgcaat
//
