LOCUS       sequence-371125-        9242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  chromosomal integration downstream of glmS gene via Tn7 system of a 
            cassette encoding for arabinose-inducible GFP and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Avraham, Roi
  TITLE     Bacterial fluorescent reporters and tags toolkit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: R6K_BAD_GFP-mCherry_ChInt--
FEATURES             Location/Qualifiers
     source          1..9242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  152..317
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     terminator      complement(491..518)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(610..696)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(927..1637)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYMKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1205..1224)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1474..1492
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     RBS             1646..1654
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(1681..1965)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             1992..2870
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(3050..3069)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     terminator      complement(3126..3153)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3245..3331)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3653..4366)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMTDKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3693..3713)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     4308..4336
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             4375..4383
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(4410..4694)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     protein_bind    complement(4990..5037)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(5114..5119,5120..5136,5137..5142)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5253..6047
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5307..5326)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5917..5936
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(6099..6146)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(6197..6283)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(6386..6480)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(6508..6524)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6509..6525)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6939..6958)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7058..7080
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7118..7136)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7204..7308
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7309..7377,7378..8169)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7527..7546)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8470..8858
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            9127..9235
                     /note="incP origin of transfer"
ORIGIN
        1 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcgg gtcagtgagc gaggaagcgg
       61 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg
      121 cccaaacata acaggaagaa aaatgccccg ctgtgggcgg acaaaatagt tgggaactgg
      181 gaggggtgga aatggagttt ttaaggatta tttagggaag agtgacaaaa tagatgggaa
      241 ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg acaaaatagt ttggaactag
      301 atttcactta tctggttctt gaggcggtta aaagagccgt actcttctcc gatgtcgact
      361 aggccatgat gctcattctg tgggaccaaa acgaaaaaac accctttcgg gtgtcttttc
      421 tggaatttgg tacgcggata catatttgaa tgtatttaga aaaataaaca aaagagtttg
      481 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttaatttgat gcctggcagt
      541 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc
      601 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
      661 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
      721 catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag ttcggcatgg
      781 ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt ctgttttatc agaccgcttc
      841 tgcgttctga tttaatctgt atcaggctga aaatcttctc tcatccgcca aaacagccaa
      901 gcttgcatgc ctgcaggtcg actctattac ttgtacagct cgtccatgcc gccggtggag
      961 tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg
     1021 atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg
     1081 taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg
     1141 atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc
     1201 atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc
     1261 ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg
     1321 tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcatgtag
     1381 tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac
     1441 aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg
     1501 gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg
     1561 gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat gttatcctcc
     1621 tcgcccttgc tcaccatttt ttataacctc cttacctcct gctagcccaa aaaaacgggt
     1681 atggagaaac agtagagagt tgcgataaaa agcgtcaggt aggatccgct aatcttatgg
     1741 ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa ataatcaatg tggacttttc
     1801 tgccgtgatt atagacactt ttgttacgcg tttttgtcat ggctttggtc ccgctttgtt
     1861 acagaatgct tttaataagc ggggttaccg gtttggttag cgagaagagc cagtaaaaga
     1921 cgcagtgacg gcaatgtctg atgcaatatg gacaattggt ttcttctctg aatggcggga
     1981 gtatgaaaag tatggctgaa gcgcaaaatg atcccctgct gccgggatac tcgtttaatg
     2041 cccatctggt ggcgggttta acgccgattg aggccaacgg ttatctcgat ttttttatcg
     2101 accgaccgct gggaatgaaa ggttatattc tcaatctcac cattcgcggt cagggggtgg
     2161 tgaaaaatca gggacgagaa tttgtttgcc gaccgggtga tattttgctg ttcccgccag
     2221 gagagattca tcactacggt cgtcatccgg aggctcgcga atggtatcac cagtgggttt
     2281 actttcgtcc gcgcgcctac tggcatgaat ggcttaactg gccgtcaata tttgccaata
     2341 cggggttctt tcgcccggat gaagcgcacc agccgcattt cagcgacctg tttgggcaaa
     2401 tcattaacgc cgggcaaggg gaagggcgct attcggagct gctggcgata aatctgcttg
     2461 agcaattgtt actgcggcgc atggaagcga ttaacgagtc gctccatcca ccgatggata
     2521 atcgggtacg cgaggcttgt cagtacatca gcgatcacct ggcagacagc aattttgata
     2581 tcgccagcgt cgcacagcat gtttgcttgt cgccgtcgcg tctgtcacat cttttccgcc
     2641 agcagttagg gattagcgtc ttaagctggc gcgaggacca acgtatcagc caggcgaagc
     2701 tgcttttgag caccacccgg atgcctatcg ccaccgtcgg tcgcaatgtt ggttttgacg
     2761 atcaactcta tttctcgcgg gtatttaaaa aatgcaccgg ggccagcccg agcgagttcc
     2821 gtgccggttg tgaagaaaaa gtgaatgatg tagccgtcaa gttgtcataa ttggtaacga
     2881 atcagacaat tgacggcttg acggagtagc atagggtttg cagaatccct gcttcgtcca
     2941 tttgacaggc acattatgca tcgatgataa gctgtcaaac atgagcagat cctctacgcc
     3001 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     3061 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcag agtttgtaaa
     3121 acgcaaaaag gccatccgtc aggatggcct tctgcttaat ttgatgcctg gcagtttatg
     3181 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa tccgctcccg
     3241 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3301 agtctttcga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcatgg
     3361 ggagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca
     3421 ggtgggacca ccgcgctact gccgccaggc aaattctgtt ttatcagacc gcttctgcgt
     3481 tctgatttaa tctgtatcag gctgaaaatc ttctctcatc cgccaaaaca gccaagcttg
     3541 catgcctgca ggtcgactct agtgataaac taccgcatta aagcttgcat gcctgcaggt
     3601 ctggacattt tttttttttt tttttttttt ttttttttta tgtccgcctt tatttgtata
     3661 gttcatccat gccatgtgta atcccagcag ctgttacaaa ctcaagaagg accatgtggt
     3721 ctctcttttc gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa tggttgtctg
     3781 gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa
     3841 cgcttccatc ttcaatgttg tgtctaattt tgaagttaac tttgattcca ttcttttgtt
     3901 tgtctgtcat gatgtataca ttgtgtgagt tatagttgta ttccaatttg tgtccaagaa
     3961 tgtttccatc ttctttaaaa tcaatacctt ttaactcgat tctattaaca agggtatcac
     4021 cttcaaactt gacttcagca cgtgtcttgt agttcccgtc atctttgaaa aatatagttc
     4081 tttcctgtac ataaccttcg ggcatggcac tcttgaaaaa gtcatgctgt ttcatatgat
     4141 ctgggtatct cgcaaagcat tgaacaccat aaccgaaagt agtgacaagt gttggccatg
     4201 gaacaggtag ttttccagta gtgcaaataa atttaagggt aagttttccg tatgttgcat
     4261 caccttcacc ctctccactg acagaaaatt tgtgcccatt aacatcacca tctaattcaa
     4321 caagaattgg gacaactcca gtgaaaagtt cttctccttt actcattttt tataacctcc
     4381 ttacctcctg ctagcccaaa aaaacgggta tggagaaaca gtagagagtt gcgataaaaa
     4441 gcgtcaggta ggatccgcta atcttatgga taaaaatgct atggcatagc aaagtgtgac
     4501 gccgtgcaaa taatcaatgt ggacttttct gccgtgatta tagacacttt tgttacgcgt
     4561 ttttgtcatg gctttggtcc cgctttgtta cagaatgctt ttaataagcg gggttaccgg
     4621 tttggttagc gagaagagcc agtaaaagac gcagtgacgg caatgtctga tgcaatatgg
     4681 acaattggtt tcttgcttgt ttcggcgtgg gtatgaattc atgtggctga ccgttctgtt
     4741 gtctctcgct cttccgagta gacgaacaat aaggcctccc taacgggggg ccttttttat
     4801 tgataacaaa agtcagtgct tccgctattt ccaaaatacc gggctaatac ggtttaaacg
     4861 acctcctgga tttgctcaga cagccttttc gtcattcgtt tcagccaaaa aacttaagac
     4921 cgccggtctt gtccactacc ttgcagtaat gcggtggaca ggatcggcgg ttttcttttc
     4981 tcttctcaag aagttcctat actttctaga gaataggaac ttcggaatag gaacttcctc
     5041 ctgaacggcc ataagaacga aggctgtctg ttgaactctc gagccgtgga aacggatgaa
     5101 ggcacgaacc cagttgacat aagcctgttc ggttcgtaaa ctgtaatgca agtagcgtat
     5161 gcgctcaaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
     5221 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     5281 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     5341 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     5401 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     5461 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     5521 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     5581 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     5641 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     5701 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     5761 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     5821 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     5881 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     5941 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     6001 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     6061 gttcgaaatg actcgagcat cagcttcaaa agcgctctga agttcctata ctttctagag
     6121 aataggaact tcggaatagg tacttcaaga tccccaattc gagctcatcg tccgggccgc
     6181 aagctcctag cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     6241 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctc aagctagaga
     6301 gtcattaccc caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc
     6361 tgccactcat cgcagtctag cttggattct caccaataaa aaacgcccgg cggcaaccga
     6421 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     6481 caagctcagc taattaagct agcttatcga taccgtcgac ctcgaacccc acgcccctct
     6541 ttaatacgac gggcaatttg cacttcagaa aatgaagagt ttgctttagc cataacaaaa
     6601 gtccagtatg ctttttcaca gcataactgg actgatttca gtttacaact attctgtcta
     6661 gtttaagact ttattgtcat agtttagatc tattttgttc agtttaagac tttattgtcc
     6721 gcccacacct gcttacgcag ggcatccatt tattactcaa ccgtaaccga ttttgccagg
     6781 ttacgcggct ggtcctctag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6841 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     6901 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     6961 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     7021 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     7081 ttttcaccgt cgtcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     7141 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     7201 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7261 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7321 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7381 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7441 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7501 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7561 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7621 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7681 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7741 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7801 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7861 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7921 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7981 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtcccg cggtatcatt
     8041 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8101 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8161 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8221 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8281 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggcagttat
     8341 tggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8401 gattgattta aaacttcatt tttaattttt gcggccgcaa gatccggcca cgatgcgtcc
     8461 ggcgtagagg atctgaagat cagcagttca acctgttgat agtacgtact aagctctcat
     8521 gtttcacgta ctaagctctc atgtttaacg tactaagctc tcatgtttaa cgaactaaac
     8581 cctcatggct aacgtactaa gctctcatgg ctaacgtact aagctctcat gtttcacgta
     8641 ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa tagcctctaa
     8701 ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagctt ttaaggttta
     8761 acggttgtgg acaacaagcc agggatgtaa cgcactgaga agcccttaga gcctctcaaa
     8821 gcaattttga gtgacacagg aacacttaac ggctgacatg ggaattagcc atggcatcac
     8881 agtatcgtga tgacagaggc agggagtgcg gcctttttac ggttcctggc cttttgctgg
     8941 ccttttgctc acatgttctt tcctgcgtta tcaggggatt ccttaaggta tactttccgc
     9001 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc
     9061 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg
     9121 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt
     9181 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccgataag
     9241 ct
//
