LOCUS       sequence-371128-        9242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  chromosomal integration downstream of glmS gene via Tn7 system of a 
            cassette encoding for arabinose-inducible GFP and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Avraham, Roi
  TITLE     Bacterial fluorescent reporters and tags toolkit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: R6K_BAD_GFP-mCherry_ChInt++
FEATURES             Location/Qualifiers
     source          1..9242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  152..317
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        455..739
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     RBS             766..774
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             783..1496
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMTDKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(813..841)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1436..1456
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1818..1904
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1996..2023
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     2080..2099
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(2279..3157)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3184..3468
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     RBS             3495..3503
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             3512..4222
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYMKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3657..3675)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3925..3944
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      4453..4539
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4631..4658
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    complement(4990..5037)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(5114..5119,5120..5136,5137..5142)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5253..6047
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5307..5326)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5917..5936
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(6099..6146)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(6197..6283)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(6386..6480)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(6508..6524)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6509..6525)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6939..6958)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7058..7080
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7118..7136)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7204..7308
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7309..7377,7378..8169)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7527..7546)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8470..8858
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            9127..9235
                     /note="incP origin of transfer"
ORIGIN
        1 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcgg gtcagtgagc gaggaagcgg
       61 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg
      121 cccaaacata acaggaagaa aaatgccccg ctgtgggcgg acaaaatagt tgggaactgg
      181 gaggggtgga aatggagttt ttaaggatta tttagggaag agtgacaaaa tagatgggaa
      241 ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg acaaaatagt ttggaactag
      301 atttcactta tctggttctt gaggcggtta aaagagccgt actcttctcc gatgtcgact
      361 aggccatgat gctcattctg tgggaccaaa acgaaaaaac accctttcgg gtgtcttttc
      421 tggaatttgg taccataccc acgccgaaac aagcaagaaa ccaattgtcc atattgcatc
      481 agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg
      541 cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa
      601 agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc
      661 tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa
      721 ctctctactg tttctccata cccgtttttt tgggctagca ggaggtaagg aggttataaa
      781 aaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      841 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      901 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      961 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
     1021 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
     1081 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
     1141 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     1201 aattggaata caactataac tcacacaatg tatacatcat gacagacaaa caaaagaatg
     1261 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     1321 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     1381 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     1441 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataaa
     1501 ggcggacata aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa atgtccagac ctgcaggcat
     1561 gcaagcttta atgcggtagt ttatcactag agtcgacctg caggcatgca agcttggctg
     1621 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
     1681 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
     1741 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt
     1801 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1861 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
     1921 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
     1981 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt tacaaactct
     2041 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
     2101 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
     2161 ctgctcatgt ttgacagctt atcatcgatg cataatgtgc ctgtcaaatg gacgaagcag
     2221 ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt
     2281 atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg
     2341 ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa
     2401 cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc
     2461 tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat
     2521 gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc
     2581 tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg
     2641 gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta
     2701 tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca
     2761 ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata
     2821 ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt
     2881 gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca
     2941 gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac
     3001 cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat
     3061 cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt
     3121 atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca
     3181 gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc
     3241 tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga
     3301 ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca
     3361 ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag
     3421 cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt
     3481 gggctagcag gaggtaagga ggttataaaa aatggtgagc aagggcgagg aggataacat
     3541 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     3601 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3661 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     3721 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acatgaagct
     3781 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     3841 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     3901 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     3961 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     4021 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     4081 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     4141 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     4201 catggacgag ctgtacaagt aatagagtcg acctgcaggc atgcaagctt ggctgttttg
     4261 gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
     4321 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
     4381 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
     4441 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     4501 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     4561 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     4621 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
     4681 tttatttttc taaatacatt caaatatgta tccgcaattc atgtggctga ccgttctgtt
     4741 gtctctcgct cttccgagta gacgaacaat aaggcctccc taacgggggg ccttttttat
     4801 tgataacaaa agtcagtgct tccgctattt ccaaaatacc gggctaatac ggtttaaacg
     4861 acctcctgga tttgctcaga cagccttttc gtcattcgtt tcagccaaaa aacttaagac
     4921 cgccggtctt gtccactacc ttgcagtaat gcggtggaca ggatcggcgg ttttcttttc
     4981 tcttctcaag aagttcctat actttctaga gaataggaac ttcggaatag gaacttcctc
     5041 ctgaacggcc ataagaacga aggctgtctg ttgaactctc gagccgtgga aacggatgaa
     5101 ggcacgaacc cagttgacat aagcctgttc ggttcgtaaa ctgtaatgca agtagcgtat
     5161 gcgctcaaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
     5221 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     5281 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     5341 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     5401 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     5461 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     5521 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     5581 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     5641 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     5701 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     5761 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     5821 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     5881 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     5941 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     6001 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     6061 gttcgaaatg actcgagcat cagcttcaaa agcgctctga agttcctata ctttctagag
     6121 aataggaact tcggaatagg tacttcaaga tccccaattc gagctcatcg tccgggccgc
     6181 aagctcctag cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     6241 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctc aagctagaga
     6301 gtcattaccc caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc
     6361 tgccactcat cgcagtctag cttggattct caccaataaa aaacgcccgg cggcaaccga
     6421 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     6481 caagctcagc taattaagct agcttatcga taccgtcgac ctcgaacccc acgcccctct
     6541 ttaatacgac gggcaatttg cacttcagaa aatgaagagt ttgctttagc cataacaaaa
     6601 gtccagtatg ctttttcaca gcataactgg actgatttca gtttacaact attctgtcta
     6661 gtttaagact ttattgtcat agtttagatc tattttgttc agtttaagac tttattgtcc
     6721 gcccacacct gcttacgcag ggcatccatt tattactcaa ccgtaaccga ttttgccagg
     6781 ttacgcggct ggtcctctag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6841 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     6901 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     6961 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     7021 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     7081 ttttcaccgt cgtcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     7141 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     7201 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7261 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7321 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7381 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7441 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7501 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     7561 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7621 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7681 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7741 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7801 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7861 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7921 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7981 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtcccg cggtatcatt
     8041 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8101 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8161 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8221 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8281 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggcagttat
     8341 tggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8401 gattgattta aaacttcatt tttaattttt gcggccgcaa gatccggcca cgatgcgtcc
     8461 ggcgtagagg atctgaagat cagcagttca acctgttgat agtacgtact aagctctcat
     8521 gtttcacgta ctaagctctc atgtttaacg tactaagctc tcatgtttaa cgaactaaac
     8581 cctcatggct aacgtactaa gctctcatgg ctaacgtact aagctctcat gtttcacgta
     8641 ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa tagcctctaa
     8701 ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagctt ttaaggttta
     8761 acggttgtgg acaacaagcc agggatgtaa cgcactgaga agcccttaga gcctctcaaa
     8821 gcaattttga gtgacacagg aacacttaac ggctgacatg ggaattagcc atggcatcac
     8881 agtatcgtga tgacagaggc agggagtgcg gcctttttac ggttcctggc cttttgctgg
     8941 ccttttgctc acatgttctt tcctgcgtta tcaggggatt ccttaaggta tactttccgc
     9001 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc
     9061 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg
     9121 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt
     9181 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccgataag
     9241 ct
//
