LOCUS       sequence-37113-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Burkholderia allelic exchange suicide vector pMo130, ref EU862243.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI
  TITLE     An allelic exchange system for compliant genetic manipulation of the
            select agents Burkholderia pseudomallei and Burkholderia mallei.
  JOURNAL   Gene. 2009 Feb 1. 430(1-2):123-31.
  PUBMED    19010402
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2009 
            Feb 1. 430(1-2):123-31."
COMMENT     Sequence Label: pMo130
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(29..132)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     200..218
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(256..278)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctgcgtgcaa tccatcttgt tcaatcatac tcttcctttt tcaaattatt gaagcattta
       61 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
      121 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      181 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      241 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      301 agcggatgcc gggagcagac aagcccgtcg catgcatcta gagggatccg ctgcagggcg
      361 gccgctcagg tcagcacggt catgaatcgt tcgttgagaa tgcggtcgtg gtaaaagatc
      421 gccttgccca gctggtcggt ggtccaggtc accggtttgt ggtccgggta gttgtaatct
      481 cccccgcaga acacttcgtt gcggttaccg gacgggtcga agaagtagat ggtcttgccg
      541 tgagtgaggc cgtggcgggt tgggccgata tcgatagatg tgtcggtcat ggagatcagg
      601 tcggcggcgc gaagcaagtc ttcccaggtt tcgaggtgga aggacacatg atggaggcgg
      661 cctttttccg gatggtgaat gaaggccacg tcgtgggcct tggtcgacag actgagaaac
      721 tgggcgacgc gcgtg
//
