LOCUS       sequence-371138-        6883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant
            retrovirus providing efficient gene transfers and expression of the 
            HPV16 E6 and E7 genes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Halbert CL, Demers GW, Galloway DA
  TITLE     The E7 gene of human papillomavirus type 16 is sufficient for 
            immortalization of human epithelial cells.
  JOURNAL   J Virol. 1991 Jan;65(1):473-8.
  PUBMED    1845902
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "1991-01"; volume: "65(1)"; pages: "473-8"
COMMENT     Sequence Label: pLXSN16E6E7
FEATURES             Location/Qualifiers
     source          1..6883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             588..763
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    826..1025
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1226..1642
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1572..1594
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1610..1626
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2512..2532)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2517..2846
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2697..2832
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2906..3700
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2960..2979)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3570..3589
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             3929..4522
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4625..4647)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4733..4873
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4788..4807
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4888..4905)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5059..5647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5139..5158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5818..6609,6610..6678))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6441..6460
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6679..6783)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6851..6869
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
       61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
      121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
      181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
      241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
      301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
      361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
      421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
      481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
      541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
      601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
      661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
      721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
      781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
      841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
      901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
      961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
     1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
     1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
     1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
     1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
     1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
     1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
     1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
     1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
     1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
     1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
     1621 cctcactcct tctctaggcg ccggaattcg agctcggtac ccccggttag tataaaagca
     1681 gacattttat gcaccaaaag agaactgcaa tgtttcagga cccacaggag cgacccagaa
     1741 agttaccaca gttatgcaca gagctgcaaa caactataca tgatataata ttagaatgtg
     1801 tgtactgcaa gcaacagtta ctgcgacgtg aggtatatga ctttgctttt cgggatttat
     1861 gcatagtata tagagatggg aatccatatg ctgtatgtga taaatgttta aagttttatt
     1921 ctaaaattag tgagtataga cattattgtt atagtttgta tggaacaaca ttagaacagc
     1981 aatacaacaa accgttgtgt gatttgttaa ttaggtgtat taactgtcaa aagccactgt
     2041 gtcctgaaga aaagcaaaga catctggaca aaaagcaaag attccataat ataaggggtc
     2101 ggtggaccgg tcgatgtatg tcttgttgca gatcatcaag aacacgtaga gaaacccagc
     2161 tgtaatcatg catggagata cacctacatt gcatgaatat atgttagatt tgcaaccaga
     2221 gacaactgat ctctactgtt atgagcaatt aaatgacagc tcagaggagg aggatgaaat
     2281 agatggtcca gctggacaag cagaaccgga cagagcccat tacaatattg taaccttttg
     2341 ttgcaagtgt gactctacgc ttcggttgtg cgtacaaagc acacacgtag acattcgtac
     2401 tttggaagac ctgttaatgg gcacactagg aattgtgtgc cccatctgtt ctcagaaacc
     2461 ataatctacc atggctgatc ctgcaggcat gcaagctggg gatccggctg tggaatgtgt
     2521 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2581 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     2641 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     2701 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     2761 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     2821 tttttggagg cctaggcttt tgcaaaaagc ttgggctgca ggtcgaggcg gatctgatca
     2881 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     2941 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     3001 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     3061 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     3121 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     3181 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     3241 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     3301 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     3361 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     3421 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     3481 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     3541 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     3601 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     3661 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     3721 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     3781 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     3841 ggggatctca tgctggagtt cttcgcccac cccaacacga gccatagata aaataaaaga
     3901 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     3961 tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt
     4021 tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     4081 taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa
     4141 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
     4201 agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
     4261 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
     4321 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg
     4381 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
     4441 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
     4501 ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg
     4561 accaccgacc caccaccggg aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg
     4621 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     4681 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     4741 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     4801 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     4861 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4921 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4981 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5041 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5461 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     5521 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5581 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5641 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5701 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5761 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5821 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5881 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5941 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     6001 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6061 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     6121 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     6181 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6241 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6301 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6361 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6421 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6481 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6541 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6601 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6661 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6721 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6781 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     6841 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caa
//
