LOCUS       sequence-371147-        8452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Tauzin A, Nicolas A, Ding S, Benlarbi M, Medjahed H, Chatterjee D, 
            Dionne K, Gong SY, Gendron-Lepage G, Bo Y, Perreault J, Goyette G, 
            Gokool L, Arlotto P, Morrisseau C, Tremblay C, Martel-Laferriere V, 
            De Serres G, Levade I, Kaufmann DE, Cote M, Bazin R, Finzi A
  TITLE     Spike recognition and neutralization of SARS-CoV-2 Omicron 
            subvariants elicited after the third dose of mRNA vaccine.
  JOURNAL   Cell Rep. 2023 Jan 31;42(1):111998. doi: 
            10.1016/j.celrep.2023.111998. Epub 2023 Jan 9.
  PUBMED    36656710
REFERENCE   2  (bases 1 to 8452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-01-31"; volume: "42(1):111998. doi"; pages: " 
            10.1016/j.celrep.2023.111998. Epub 2023 Jan 9"
COMMENT     Sequence Label: pCAGGS SARS-CoV-2 BA.2.75 Spike
FEATURES             Location/Qualifiers
     source          1..8452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          73..1089
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1013..1035
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1097..1116
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(4972..4991)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5037..5092
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5091..5110)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5453..5469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5453..5469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5466..5488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5477..5493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5501..5507,5508..5525,5526..5531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5546..5567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5626..5821
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5672..5807
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5827..5961
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5864..5883)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5918..5937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6029..6046)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6200..6788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6280..6299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6959..7750,7751..7819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7582..7601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7820..7924)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7955..8334
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
       61 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
      121 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
      181 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
      241 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
      301 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
      361 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
      421 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
      481 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
      541 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
      601 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
      661 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
      721 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
      781 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
      841 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
      901 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
      961 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1021 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1081 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1141 ttcgccacca tgttcgtctt cctcgtcctc ctccccctcg tctcctccca gtgcgtcaac
     1201 ctcatcaccc gcacccagag ctacaccaac tccttcaccc gcggcgtcta ctaccccgac
     1261 aaggtcttcc gctcctccgt cctccactcc acccaggacc tcttcctccc cttcttctcc
     1321 aacgtcacct ggttccacgc catccacgtc tccggcacca acggcaccaa gcgcttcgac
     1381 aaccccgtcc tccccttcaa cgacggcgtc tacttcgcct ccaccgagaa gtccaacatc
     1441 atccgcggct ggatcttcgg caccaccctc gactccaaga cccagtccct cctcatcgtc
     1501 aacaacgcca ccaacgtcgt catcaaggtc tgcgagttcc agttctgcaa cgaccccttc
     1561 ctcgacgtct actaccacga gaacaacaag tccaggatgg agtccgagct gcgcgtctac
     1621 tcctccgcca acaactgcac cttcgagtac gtctcccagc ccttcctcat ggacctcgag
     1681 ggcaagcagg gcaacttcaa gaacctccgc gagttcgtct tcaagaacat cgacggctac
     1741 ttcaagatct actccaagca cacccccgtg aacctcggcc gcgacctccc ccagggcttc
     1801 tccgccctcg agcccctcgt cgacctcccc atcggcatca acatcacccg cttccagacc
     1861 ctcctcgccc tccaccgctc ctacctcacc cccggcgact cctcctccag ctggaccgcc
     1921 ggcgccgccg cctactacgt cggctacctc cagccccgca ccttcctcct caagtacaac
     1981 gagaacggca ccatcaccga cgccgtcgac tgcgccctcg accccctctc cgagaccaag
     2041 tgcaccctca agtccttcac cgtcgagaag ggcatctacc agacctccaa cttccgcgtc
     2101 cagcccaccg agtccatcgt ccgcttcccc aacatcacca acctctgccc cttcagagag
     2161 gtcttcaacg ccacccgctt cgcctccgtc tacgcctgga accgcaagcg catctccaac
     2221 tgcgtcgccg actactccgt cctctacaac ttcgcccctt tcttcgcctt caagtgctac
     2281 ggcgtctccc ccaccaagct caacgacctc tgcttcacca acgtctacgc cgactccttc
     2341 gtcatccgcg gcaacgaggt cagccagatc gcccccggcc agaccggcaa catcgccgac
     2401 tacaactaca agctccccga cgacttcacc ggctgcgtca tcgcctggaa ctccaacaag
     2461 ctcgactcca aggtcagcgg caactacaac tacctctacc gcctcttccg caagtccaag
     2521 ctcaagccct tcgagcgcga catctccacc gagatctacc aggccggcaa caaaccctgc
     2581 aacggcgtcg ccggcttcaa ctgctacttc cccctccagt cctacggctt caggcccacc
     2641 tacggcgtcg gccaccagcc ctaccgcgtc gtcgtcctct ccttcgagct cctccacgcc
     2701 cccgccaccg tctgcggccc caagaagtcc accaacctcg tcaagaacaa gtgcgtcaac
     2761 ttcaacttca acggcctcac cggcaccggc gtcctcaccg agtccaacaa gaagttcctc
     2821 cccttccagc agttcggccg cgacatcgcc gacaccaccg acgccgtccg cgacccccag
     2881 accctcgaga tcctcgacat caccccctgc tccttcggcg gcgtctccgt catcaccccc
     2941 ggcaccaaca cctccaacca ggtcgccgtc ctctaccagg gtgtcaactg caccgaggtc
     3001 cccgtcgcca tccacgccga ccagctcacc cccacctggc gcgtctactc caccggctcc
     3061 aacgtcttcc agacccgcgc cggctgcctc atcggcgccg agtacgtcaa caactcctac
     3121 gagtgcgaca tccccatcgg cgccggcatc tgcgcctcct accagaccca gaccaagtcc
     3181 caccgccgcg cccgctccgt cgcctcccag tccatcatcg cctacaccat gtccctcggc
     3241 gccgagaact ccgtcgccta ctccaacaac tccatcgcca tccccaccaa cttcaccatc
     3301 tccgtcacca ccgagatcct ccccgtctcc atgaccaaga cctccgtcga ctgcaccatg
     3361 tacatctgcg gcgactccac cgagtgctcc aacctcctcc tccagtacgg ctccttctgc
     3421 acccagctca agcgcgccct caccggcatc gccgtcgagc aggacaagaa cacccaggag
     3481 gtcttcgccc aggtcaagca gatctacaag acccccccca tcaagtactt cggcggcttc
     3541 aacttctccc agatcctccc cgacccctcc aagccctcca agcgctcctt catcgaggac
     3601 ctcctcttca acaaggtcac cctcgccgac gccggcttca tcaagcagta cggcgactgc
     3661 ctcggcgaca tcgccgcccg cgacctcatc tgcgcccaga agttcaacgg cctcaccgtc
     3721 ctcccccccc tcctcaccga cgagatgatc gcccagtaca cctccgccct cctcgccggc
     3781 accatcacct ccggctggac cttcggcgcc ggcgccgccc tccagatccc cttcgccatg
     3841 cagatggcct accgcttcaa cggcatcggc gtcacccaga acgtcctcta cgagaaccag
     3901 aagctcatcg ccaaccagtt caactccgcc atcggcaaga tccaggactc cctctcctcc
     3961 accgcctccg ccctcggcaa gctccaggac gtcgtcaacc acaacgccca ggccctcaac
     4021 accctcgtca agcagctctc ctccaagttc ggcgccatct cctccgtcct caacgacatc
     4081 ctgtcccgcc tcgacaaggt cgaggccgag gtccagatcg accgcctcat caccggccgc
     4141 ctccagtccc tccagaccta cgtcacccag cagctcatcc gcgccgccga gatccgcgcc
     4201 tccgccaacc tcgccgccac caagatgtcc gagtgcgtcc tcggccagtc caagcgcgtc
     4261 gacttctgcg gcaagggcta ccacctcatg tccttccccc agtccgcccc ccacggcgtc
     4321 gtcttcctcc acgtcaccta cgtccccgcc caggagaaga acttcaccac cgcccccgcc
     4381 atctgccacg acggcaaggc ccacttcccc cgcgagggcg tcttcgtctc caacggcacc
     4441 cactggttcg tcacccagcg caacttctac gagccccaga tcatcaccac cgacaacacc
     4501 ttcgtctccg gcaactgcga cgtcgtcatc ggcatcgtca acaacaccgt ctacgacccc
     4561 ctccagcccg agctcgactc cttcaaggag gagctcgaca agtacttcaa gaaccacacc
     4621 tcccccgacg tcgacctcgg cgacatctcc ggcatcaacg cctccgtcgt caacatccag
     4681 aaggagatcg accgcctcaa cgaggtcgcc aagaacctca acgagtccct catcgacctc
     4741 caggagctcg gcaagtacga gcagtacatc aagtggccct ggtacatctg gctcggcttc
     4801 atcgccggcc tcatcgccat cgtcatggtc accatcatgc tctgctgcat gacctcctgc
     4861 tgctcctgcc tcaagggctg ctgctcctgc ggctcctgct gcaagttcga cgaggacgac
     4921 tccgagcccg tcctcaaggg cgtcaagctc cactacacct aagctagcag atctttttcc
     4981 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     5041 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     5101 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     5161 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     5221 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     5281 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     5341 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     5401 tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
     5461 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5521 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5581 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
     5641 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5701 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5761 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5821 aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5881 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5941 gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6001 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6061 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7921 cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa
     7981 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     8041 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     8101 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     8161 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     8221 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     8281 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
     8341 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
     8401 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gg
//
