LOCUS       sequence-371148-       11755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses tdPP7-3xmCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 11755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-5- 5";
            volume: "165"; issue: "4"; pages: "976-89"
COMMENT     Sequence Label: pHR-tdPP7-3xmCherry
FEATURES             Location/Qualifiers
     source          1..11755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(302..321)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(535..554)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     654..676
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(714..732)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        800..904
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(905..973,974..1765)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1123..1142)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1936..2524
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2425..2444
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2678..2695
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2765..2785)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2770..3099
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2950..3085
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4233..4298
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4428..4448
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4873..5007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4910..4929)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4964..4983
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5175..5808
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5855..5980
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6477..6710
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6895..6939
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7224..7341
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7494..7901
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             8800..9504
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             9550..9711
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             9736..10440
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             10486..10647
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             10705..11409
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     LTR             11491..11724
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(join(11749..11755,1..12))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(join(11750..11755,1..12))
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
       61 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
      121 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
      181 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
      241 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
      301 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
      361 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
      421 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
      481 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
      541 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
      601 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
      661 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
      721 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      781 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      841 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      901 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      961 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     1021 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1081 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1141 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1201 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1261 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1321 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1381 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1441 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1501 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1561 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1621 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1681 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1741 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1801 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1861 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1921 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1981 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2041 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2101 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2161 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2221 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2281 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2341 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2401 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2461 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2521 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2581 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2641 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2701 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     2761 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     2821 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     2881 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     2941 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3001 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3061 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
     3121 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     3181 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     3241 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     3301 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     3361 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
     3421 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     3481 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     3541 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     3601 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     3661 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     3721 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     3781 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     3841 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     3901 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     3961 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     4021 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     4081 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     4141 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     4201 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     4261 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     4321 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     4381 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     4441 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     4501 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     4561 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     4621 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     4681 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     4741 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     4801 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     4861 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     4921 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     4981 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     5041 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     5101 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     5161 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
     5221 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     5281 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     5341 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     5401 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     5461 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     5521 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     5581 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     5641 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5701 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5761 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     5821 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     5881 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     5941 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     6001 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     6061 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     6121 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     6181 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     6241 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     6301 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
     6361 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     6421 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     6481 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     6541 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     6601 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     6661 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     6721 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     6781 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     6841 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     6901 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     6961 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     7021 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     7081 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     7141 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
     7201 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     7261 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     7321 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggatc
     7381 gataagcttg atatcgaatt cctgcagccc cgataaaata aaagatttta tttagtctcc
     7441 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct gcagtaacgc
     7501 cattttgcaa ggcatggaaa aataccaaac caagaataga gaagttcaga tcaagggcgg
     7561 gtacatgaaa atagctaacg ttgggccaaa caggatatct gcggtgagca gtttcggccc
     7621 cggcccgggg ccaagaacag atggtcaccg cagtttcggc cccggcccga ggccaagaac
     7681 agatggtccc cagatatggc ccaaccctca gcagtttctt aagacccatc agatgtttcc
     7741 aggctccccc aaggacctga aatgaccctg cgccttattt gaattaacca atcagcctgc
     7801 ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta taaaagagct cacaacccct
     7861 cactcggcgc gccagtcctc cgacagactg agtcgcccgg gggggatctg gagctctcga
     7921 gaattctcac gcgccggatc cgccaccatg tccaaaacca tcgttctttc ggtcggcgag
     7981 gctactcgca ctctgactga gatccagtcc accgcagacc gtcagatctt cgaagagaag
     8041 gtcgggcctc tggtgggtcg gctgcgcctc acggcttcgc tccgtcaaaa cggagccaag
     8101 accgcgtatc gcgtcaacct aaaactggat caggcggacg tcgttgattg ctccaccagc
     8161 gtctgcggcg agcttccgaa agtgcgctac actcaggtat ggtcgcacga cgtgacaatc
     8221 gttgcgaata gcaccgaggc ctcgcgcaaa tcgttgtacg atttgaccaa gtccctcgtc
     8281 gcgacctcgc aggtcgaaga tcttgtcgtc aaccttgtgc cgctgggccg tcgtgcggac
     8341 ccgctagcct cctgcggccg ctccaaaacc atcgttcttt cggtcggcga ggctactcgc
     8401 actctgactg agatccagtc caccgcagac cgtcagatct tcgaagagaa ggtcgggcct
     8461 ctggtgggtc ggctgcgcct cacggcttcg ctccgtcaaa acggagccaa gaccgcgtat
     8521 cgcgtcaacc taaaactgga tcaggcggac gtcgttgatt gctccaccag cgtctgcggc
     8581 gagcttccga aagtgcgcta cactcaggta tggtcgcacg acgtgacaat cgttgcgaat
     8641 agcaccgagg cctcgcgcaa atcgttgtac gatttgacca agtccctcgt cgcgacctcg
     8701 caggtcgaag atcttgtcgt caaccttgtg ccgctgggcc gtcgtgcgga cccgctagcc
     8761 tccacgcgtg attcgactag tggaggaagc ggaggaggag tgagcaaggg cgaggaggat
     8821 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     8881 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     8941 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     9001 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     9061 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     9121 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     9181 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     9241 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     9301 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     9361 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     9421 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     9481 ggcggcatgg acgagctgta caagcatggc atggatgagc tctacaaagg tggaggtcgg
     9541 accgaagagt acaagcttat cctgaacggt aaaaccctga aaggtgaaac caccaccgaa
     9601 gctgttgacg ctgctaccgc ggaaaaagtt ttcaaacagt acgctaacga caacggtgtt
     9661 gacggtgaat ggacctacga cgacgctacc aaaaccttca cggtaaccga agctagtgga
     9721 ggaagcggag gaggagtgag caagggcgag gaggataaca tggccatcat caaggagttc
     9781 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     9841 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     9901 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     9961 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
    10021 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
    10081 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
    10141 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
    10201 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
    10261 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
    10321 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
    10381 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
    10441 catggcatgg atgagctcta caaaggtgga ggtcggaccg aagagtacaa gcttatcctg
    10501 aacggtaaaa ccctgaaagg tgaaaccacc accgaagctg ttgacgctgc taccgcggaa
    10561 aaagttttca aacagtacgc taacgacaac ggtgttgacg gtgaatggac ctacgacgac
    10621 gctaccaaaa ccttcacggt aaccgaagct agtggtggtg gtggtagcgg tggtggtagc
    10681 agcttaccgg tcgccgaatt cggtgtgagc aagggcgagg aggataacat ggccatcatc
    10741 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
    10801 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
    10861 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
    10921 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
    10981 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
    11041 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
    11101 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
    11161 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
    11221 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
    11281 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
    11341 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
    11401 ctgtacaagc atatgtaagc ggccctttaa gaccaatgac ttacaaggca gctgtagatc
    11461 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
    11521 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
    11581 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    11641 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    11701 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatag
//
