LOCUS       sequence-371150-       10417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses arrays of gRNA targeting hh promoter under U6b/c promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Bui M, Dalla Benetta E, Dong Y, Zhao Y, Yang T, Li M, Antoshechkin 
            IA, Buchman A, Bottino-Rojas V, James AA, Perry MW, Dimopoulos G, 
            Akbari OS
  TITLE     CRISPR mediated transactivation in the human disease vector Aedes 
            aegypti.
  JOURNAL   PLoS Pathog. 2023 Jan 19;19(1):e1010842. doi: 
            10.1371/journal.ppat.1010842. eCollection 2023 Jan.
  PUBMED    36656895
REFERENCE   2  (bases 1 to 10417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2023-01-19"; volume: "19(1):e1010842. doi"; pages: 
            " 10.1371/journal.ppat.1010842. eCollection 2023 Jan"
COMMENT     Sequence Label: OA-1053B
FEATURES             Location/Qualifiers
     source          1..10417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     99..118
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     352..369
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    486..507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(522..527,528..545,546..552)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    560..576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     565..587
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     584..600
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     584..600
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   781..815
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1483..1604
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1494..1513)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1548..1567
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2538..2561)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2736..2755)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3005..3023
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      3159..3168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   7635..7697
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8401..8418)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8401..8417)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8410..8432)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8626..8645)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8745..8767
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8805..8823)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8891..8995
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8996..9064,9065..9856)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9214..9233)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10027..10417,1..198)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
       61 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      121 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      181 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      241 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
      301 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      361 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
      421 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
      481 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
      541 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
      601 atgattacga attcgagctc ggtacccggg gatcctctag agtcgacgct cgcgcgactt
      661 ggtttgccat tctttagcgc gcgtcgcgtc acacagcttg gccacaatgt ggtttttgtc
      721 aaacgaagat tctatgacgt gtttaaagtt taggtcgagt aaagcgcaaa tcttttttaa
      781 ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt
      841 tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc
      901 ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt
      961 gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa
     1021 ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt
     1081 atagatatcg tgactaatat ataataaaat gggtagttct ttagacgatg agcatatcct
     1141 ctctgctctt ctgcaaagcg atgacgagct tgttggtgag gattctgaca gtgaaatatc
     1201 agatcacgta agtgaagatg acgtccagag cgatacagaa gaagcgttta tagatgaggt
     1261 acatgaagtg cagccaacgt caagcggtag tgaaatatta gacgaacaaa atgttattga
     1321 acaaccaggt tcttcattgg cttctaacag aatcttgacc ttgccacaga ggactattag
     1381 aggtaagaat aaacattgtt ggtcaacttc aaagtccacg aggcgtagcc gagtctctgc
     1441 actgaacatt gtcagatccg agatcggccg gcctagaagc tttaagatac attgatgagt
     1501 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     1561 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     1621 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     1681 tctacaaatg tggtatggct gattatgatc tagagtcgcg gccgcctact tgtacagctc
     1741 gtccatgccg tagaggaaca ggtgatgtcg gccttcgctg cgctcatact gctcgacaat
     1801 ggtgtagtct tcgttatgcg aagttatatc cagtttcgta tccacataat aatatccggg
     1861 cagctgaacc ggcttcttgg ccatgtaaat ggtcttaaat tcgaccaggt agtgaccacc
     1921 atctttcagt ttgagggcct gatggatctc gcccttgagc actccgtctc gagggtacag
     1981 acgttcggtg gatgcctccc aacccatggt cttcttctgc atcactgggc catctggcgg
     2041 aaagttagtg ccgcgcattt taaccttata gatcagtgta ccgtcctgca aactgctatc
     2101 ttgggtcaca gtgaccagtc ctccgtcttc gaaattcatc acgcgctccc acttgaagcc
     2161 ttccggaaag gacagctttt tgtagtccgg tatgtcagcg gggtgcttaa cgtaggcctt
     2221 gctgccgtac ataaactggg gactcaggat atcccaggcg aagggcagtg gtccgccctt
     2281 ggtgaccttg agtttggctg tttgtgtgcc ttcatacggg cgtccctcgc cctctccctc
     2341 gatctcgaac tcatgtccgt tcatggatcc ctccatccta actttgaagc gcataaattc
     2401 cttgataacg gccatgttgt tgtcctcgga ggaggcggtg ccggagctgc cgctgccggt
     2461 gctgccggtg ccatgcccca ggaacaggtg gtggcggccc tcggagcgct cgtactgttc
     2521 cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc ttggtgtcca cgtagtagta
     2581 gccgggcagt tgcacgggct tcttggccat gtagatggtc ttgaactcca ccaggtagtg
     2641 gccgccgtcc ttcagcttca gggcctggtg gatctcgccc ttcagcacgc cgtcgcgggg
     2701 gtacaggcgc tcggtggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc
     2761 gggggggaag ttggtgccgc gcatcttcac cttgtagatc agcgtgccgt cctgcaggga
     2821 ggagtcctgg gtcacggtca ccagaccgcc gtcctcgaag ttcatcacgc gctcccactt
     2881 gaagccctcg gggaaggaca gcttcttgta atcggggatg tcggcggggt gcttcacgta
     2941 cgccttggag ccgtacatga actgggggga caggatgtcc caggcgaagg gcagggggcc
     3001 gcccttggtc accttcagct tggcggtctg ggtgccctcg taggggcggc cctcgccctc
     3061 gccctcgccc tcgatctcga actcgtggcc gttcatggag ccctccatgc gcaccttgaa
     3121 gcgcatgaac tctttgatga cctcctcgcc cttgctcacc atggtggcga ccggtggatc
     3181 gtaccgtcga ctctagcggt accccgattg tttagcttgt tcagctgcgc ttgtttattt
     3241 gcttagcttt cgcttagcga cgtgttcact ttgcttgttt gaattgaatt gtcgctccgt
     3301 agacgaagcg cctctattta tactccggcg gtcgagggtt cgaaatcgat aagcttggat
     3361 cctaattgaa ttagctctaa ttgaattagt ctctaattga attagatccc cgggcgagct
     3421 cgaattggcg cgccgggccc aaaaggccga aatcggtttt ggtgtccata ctccatgtta
     3481 gtacgccaaa ggcatggcaa atacatttca aacgtacatt aatgtcaatt ttgttctcta
     3541 ccatcacaat aaatactaaa aaaatatttt tgtcaaattt ccaattagct taataatata
     3601 atatacgtac aaaattaccc ctttcagagc ccctcctggc tacaccactg gtccatcacc
     3661 cgaaaggctt tggggttttt catatttcga catgtagaat ctaacaaaaa tagtccggtt
     3721 aaaaaccccg tgtaactcca tcgtgacctt cccttgtgag aatttttgtc agaaaatact
     3781 caggaaatgt tgtacatata atttaagccc ggcaataatt tctaaagtag ctgcttgtgc
     3841 agattatgga atatgcctga aagaatttct tgaataccta attcttgaaa atttgagaac
     3901 atagtaacat ttgaagaatg ctttaccatt ccttattatt aattgccgaa gcatcttaaa
     3961 aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt atcttaactt
     4021 gctatgctgt ttccagcata gctctaagac accctgcata tcggaaccca atcaattagc
     4081 ctcattcatc ttagttatat actcaatgga aacggtaaaa gttctagcga tgggcgaaaa
     4141 tattttttcc tatccataaa gagagcttta agttagtcct ctttgacctt tttattataa
     4201 tatttgattt gacctcgttg gctttcaatg acattctttg agagaacaat gagtatctta
     4261 aacgctcaaa taaatattac ctatcgcatc tcttcatagt aaaataaatg ttccaaacag
     4321 atccgtgttt gcgattgcat atttcaaggc gtaatgtatt tttcggagga tggatgctga
     4381 caatgaaacc gtatccgtta tcaccatgga gaggtgttga agaacgaaca attaagaaga
     4441 aagttttttg ttttcggcat ttgataggat cttaccactc gaagccaatc cagcgagaca
     4501 aatagcttga agttatttag cgcatcgtta aatttttaac gactctaatt ggagctgcca
     4561 gacaaatttg atttgtccgt gcggtatgca tatgtaccta cttacgtctg tcgttttgtc
     4621 tccgtttacc gggagggaaa gtctggaaac atggaaactc tatagttgcc aggtagacca
     4681 tctgcctccg tcggctggct ggattccaat ttgaatattg gctaattgga agagatggaa
     4741 gtttttgaat ggatgattga ataattgaag cgactccggg tacctgtttg taagctctgc
     4801 aacagtgcca tagattcgtg tcagtccatc actagaatca aatcaacttg tacttgatat
     4861 ataaatggct tgggttaatg tgcttcggca agacatatcc gcggttaatt aagcgatcgc
     4921 gactcaatac tggaatcttg aaaatgttag gaactttata atgttacaat tgctgttatc
     4981 tcaagattca ttcataggat acagatttct agctaaaatt cgaaaattct aaggaaaatc
     5041 cttgttgtca aatcattata aaattcacat gcacataaag ggaatactgt aaagttacat
     5101 aaccaacata aatttcatga tcgattacgc gatgcatagc gggaatgtag taatattcat
     5161 attttcacat aatttgtcac gtgaataagt gacaaatttg gcatatatgc atgattttat
     5221 tgatttcaag agtcactctg aatggaattt tcttaacatg cagcattact ctgtactttg
     5281 aatagaattt tcttaaagta gtaggtacta catgttcgaa aaggttttta ttcataccaa
     5341 aagtgtagta aactttgtgg attttgtttt tgagtagatg ctacatgtag taaagttcaa
     5401 cgctttttat tcatacggcc cagtgccgct tatttccaag gcattcctcg ccctaacctt
     5461 atcttcgcca tgcacaacaa actgcttcac tgcaatatta ggaatcgatg aacaaccacc
     5521 aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttatcttaa
     5581 cttgctatgc tgtttccagc atagctctaa gacctttact ctctcggccg gtgatttcac
     5641 tactcttgcc tctgctctta tatagtcttc tacagcgaac gcggttctag cgatggatgg
     5701 acgtatcaac tcgatgggcg atttcattcg ttcgcacccc aatcggaacg gaacgctacc
     5761 caggggcaca catagagaag cgaaaaaaag tttagttttt gtctacctac aaaaagtcaa
     5821 acgtcccaac ttattgctga tactctaccc tacacaccta tctgccgacg atcttcaatt
     5881 ataatactgt atgtgcttac tgtaaaggat gactcatcta acaccattcc tataacgatc
     5941 aattaaatat tcaatttgtt tcaactatgt tggtcgactc gacggagtcg cacagcacag
     6001 ccgcacagtg cgttgaatgt atggagatgt ggacagaagt cataactaaa agaccagtct
     6061 ggattagtca agtctagtac acgacggcct tgcagttgag gtcgaaatac gcgtatctat
     6121 caaaggatac aaactctagt ggaattaaat tatatagcac taaattcggg ttccctttaa
     6181 cttactgttt atcgccgaaa aataccatga aatttgattt ttgtctacgg gaaaacgcac
     6241 tgtgagccgc tccgaccaca ctcgagcagt tgttaacagt tttcccagtc gagcgaagaa
     6301 taattagagt cttctggtgg aatgtcaaca ttcttgatgt gactctctgg tctgcagatt
     6361 cgatcgactg tctggcgcgc tttgcatctc tcagagcggt agtgaaatgc aaatgaaggg
     6421 ttcaattcct tgggaatcgt cgtcgtcgtc gtcgtctagg acggcgagtg tggtatatgt
     6481 tttaatttac gggattttca actcgtctcg tcacccgagc ataatttatg ccgaaagaaa
     6541 ggaatccgac tgactggtga cagatatgat gctaccgttc ccctgaagac gattataata
     6601 gtttcgtttg tgatgaagat ctcctaggag cccgggcgag ctcgaattaa ccattgtggg
     6661 aaccgtgcga tcaaacaaac gcgagatacc ggaagtactg aaaaacagtc gctccaggcc
     6721 agtgggaaca tcgatgtttt gttttgacgg accccttact ctcgtctcat ataaaccgaa
     6781 gccagctaag atggtatact tattatcatc ttgtgatgag gatgcttcta tcaacgaaag
     6841 taccggtaaa ccgcaaatgg ttatgtatta taatcaaact aaaggcggag tggacacgct
     6901 agaccaaatg tgttctgtga tgacctgcag taggaagacg aataggtggc ctatggcatt
     6961 attgtacgga atgataaaca ttgcctgcat aaattctttt attatataca gccataatgt
     7021 cagtagcaag ggagaaaagg ttcaaagtcg caaaaaattt atgagaaacc tttacatgag
     7081 cctgacgtca tcgtttatgc gtaagcgttt agaagctcct actttgaaga gatatttgcg
     7141 cgataatatc tctaatattt tgccaaatga agtgcctggt acatcagatg acagtactga
     7201 agagccagta atgaaaaaac gtacttactg tacttactgc ccctctaaaa taaggcgaaa
     7261 ggcaaatgca tcgtgcaaaa aatgcaaaaa agttatttgt cgagagcata atattgatat
     7321 gtgccaaagt tgtttctgac tgactaataa gtataatttg tttctattat gtataagtta
     7381 agctaattac ttattttata atacaacatg actgttttta aagtacaaaa taagtttatt
     7441 tttgtaaaag agagaatgtt taaaagtttt gttactttat agaagaaatt ttgagttttt
     7501 gtttttttta ataaataaat aaacataaat aaattgtttg ttgaatttat tattagtatg
     7561 taagtgtaaa tataataaaa cttaatatct attcaaatta ataaataaac ctcgatatac
     7621 agaccgataa aacacatgcg tcaattttac gcatgattat ctttaacgta cgtcacaata
     7681 tgattatctt tctagggtta aataatagtt tctaattttt ttattattca gcctgctgtc
     7741 gtgaataccg tatatctcaa cgctgtctgt gagattgtcg tattctagcc tttttagttt
     7801 ttcgctcatc gacttgatat tgtccgacac attttcgtcg atttgcgttt tgatcaaaga
     7861 cttgagcaga gacacgttaa tcaactgttc aaattgatcc atattaacga tatcaacccg
     7921 atgcgtatat ggtgcgtaaa atatattttt taaccctctt atactttgca ctctgcgtta
     7981 atacgcgttc gtgtacagac gtaatcatgt tttctttttt ggataaaact cctactgagt
     8041 ttgacctcat attagaccct cacaagttgc aaaacgtggc attttttacc aatgaagaat
     8101 ttaaagttat tttaaaaaat ttcatcacag atttaaagaa gaaccaaaaa ttaaattatt
     8161 tcaacagttt aatcgaccag ttaatcaacg tgtacacaga cgcgtcggca aaaaacacgc
     8221 agcccgacgt gttggctaaa attattaaat caacttgtgt tatagtcacg gatttgccgt
     8281 ccaacgtgtt cctcaaaaag ttgaagacca acaagtttac ggacactatt aattatttga
     8341 ttttgcccca cttcattttg tgggatcaca attttgttat atttttaaac aaagcttggc
     8401 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     8461 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     8521 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     8581 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     8641 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     8701 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     8761 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     8821 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     8881 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8941 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     9001 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     9061 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     9121 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     9181 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     9241 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     9301 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     9361 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     9421 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     9481 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     9541 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     9601 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     9661 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9721 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9781 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9841 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     9901 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9961 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    10021 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    10081 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    10141 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    10201 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    10261 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    10321 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    10381 aacgacctac accgaactga gatacctaca gcgtgag
//
