LOCUS       sequence-371151-       10411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses arrays of gRNA targeting eve promoter under U6b/c 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Bui M, Dalla Benetta E, Dong Y, Zhao Y, Yang T, Li M, Antoshechkin 
            IA, Buchman A, Bottino-Rojas V, James AA, Perry MW, Dimopoulos G, 
            Akbari OS
  TITLE     CRISPR mediated transactivation in the human disease vector Aedes 
            aegypti.
  JOURNAL   PLoS Pathog. 2023 Jan 19;19(1):e1010842. doi: 
            10.1371/journal.ppat.1010842. eCollection 2023 Jan.
  PUBMED    36656895
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2023-01-19"; volume: "19(1):e1010842. doi"; pages: 
            " 10.1371/journal.ppat.1010842. eCollection 2023 Jan"
COMMENT     Sequence Label: OA-1053A
FEATURES             Location/Qualifiers
     source          1..10411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    115..136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(151..156,157..174,175..181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    189..205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     194..216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     213..229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     213..229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   410..444
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1112..1233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1123..1142)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1177..1196
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2167..2190)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2365..2384)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2634..2652
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2782..2791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   7258..7320
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8024..8041)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8024..8040)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8033..8055)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8249..8268)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8368..8390
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8428..8446)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8514..8618
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8619..8687,8688..9479)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8837..8856)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9650..10238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10139..10158
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10392..10409
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
       61 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
      241 ttcgagctcg gtacccgggg atcctctaga gtcgacgctc gcgcgacttg gtttgccatt
      301 ctttagcgcg cgtcgcgtca cacagcttgg ccacaatgtg gtttttgtca aacgaagatt
      361 ctatgacgtg tttaaagttt aggtcgagta aagcgcaaat cttttttaac cctagaaaga
      421 tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt ctaaatagcg
      481 cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc cgtgaggcgt
      541 gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg agtcaaaatg
      601 acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat tatattgtta
      661 tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta tagatatcgt
      721 gactaatata taataaaatg ggtagttctt tagacgatga gcatatcctc tctgctcttc
      781 tgcaaagcga tgacgagctt gttggtgagg attctgacag tgaaatatca gatcacgtaa
      841 gtgaagatga cgtccagagc gatacagaag aagcgtttat agatgaggta catgaagtgc
      901 agccaacgtc aagcggtagt gaaatattag acgaacaaaa tgttattgaa caaccaggtt
      961 cttcattggc ttctaacaga atcttgacct tgccacagag gactattaga ggtaagaata
     1021 aacattgttg gtcaacttca aagtccacga ggcgtagccg agtctctgca ctgaacattg
     1081 tcagatccga gatcggccgg cctagaagct ttaagataca ttgatgagtt tggacaaacc
     1141 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     1201 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     1261 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     1321 ggtatggctg attatgatct agagtcgcgg ccgcctactt gtacagctcg tccatgccgt
     1381 agaggaacag gtgatgtcgg ccttcgctgc gctcatactg ctcgacaatg gtgtagtctt
     1441 cgttatgcga agttatatcc agtttcgtat ccacataata atatccgggc agctgaaccg
     1501 gcttcttggc catgtaaatg gtcttaaatt cgaccaggta gtgaccacca tctttcagtt
     1561 tgagggcctg atggatctcg cccttgagca ctccgtctcg agggtacaga cgttcggtgg
     1621 atgcctccca acccatggtc ttcttctgca tcactgggcc atctggcgga aagttagtgc
     1681 cgcgcatttt aaccttatag atcagtgtac cgtcctgcaa actgctatct tgggtcacag
     1741 tgaccagtcc tccgtcttcg aaattcatca cgcgctccca cttgaagcct tccggaaagg
     1801 acagcttttt gtagtccggt atgtcagcgg ggtgcttaac gtaggccttg ctgccgtaca
     1861 taaactgggg actcaggata tcccaggcga agggcagtgg tccgcccttg gtgaccttga
     1921 gtttggctgt ttgtgtgcct tcatacgggc gtccctcgcc ctctccctcg atctcgaact
     1981 catgtccgtt catggatccc tccatcctaa ctttgaagcg cataaattcc ttgataacgg
     2041 ccatgttgtt gtcctcggag gaggcggtgc cggagctgcc gctgccggtg ctgccggtgc
     2101 catgccccag gaacaggtgg tggcggccct cggagcgctc gtactgttcc acgatggtgt
     2161 agtcctcgtt gtgggaggtg atgtccagct tggtgtccac gtagtagtag ccgggcagtt
     2221 gcacgggctt cttggccatg tagatggtct tgaactccac caggtagtgg ccgccgtcct
     2281 tcagcttcag ggcctggtgg atctcgccct tcagcacgcc gtcgcggggg tacaggcgct
     2341 cggtggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg ggggggaagt
     2401 tggtgccgcg catcttcacc ttgtagatca gcgtgccgtc ctgcagggag gagtcctggg
     2461 tcacggtcac cagaccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
     2521 ggaaggacag cttcttgtaa tcggggatgt cggcggggtg cttcacgtac gccttggagc
     2581 cgtacatgaa ctggggggac aggatgtccc aggcgaaggg cagggggccg cccttggtca
     2641 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
     2701 cgaactcgtg gccgttcatg gagccctcca tgcgcacctt gaagcgcatg aactctttga
     2761 tgacctcctc gcccttgctc accatggtgg cgaccggtgg atcgtaccgt cgactctagc
     2821 ggtaccccga ttgtttagct tgttcagctg cgcttgttta tttgcttagc tttcgcttag
     2881 cgacgtgttc actttgcttg tttgaattga attgtcgctc cgtagacgaa gcgcctctat
     2941 ttatactccg gcggtcgagg gttcgaaatc gataagcttg gatcctaatt gaattagctc
     3001 taattgaatt agtctctaat tgaattagat ccccgggcga gctcgaattg gcgcgccggg
     3061 cccaaaaggc cgaaatcggt tttggtgtcc atactccatg ttagtacgcc aaaggcatgg
     3121 caaatacatt tcaaacgtac attaatgtca attttgttct ctaccatcac aataaatact
     3181 aaaaaaatat ttttgtcaaa tttccaatta gcttaataat ataatatacg tacaaaatta
     3241 cccctttcag agcccctcct ggctacacca ctggtccatc acccgaaagg ctttggggtt
     3301 tttcatattt cgacatgtag aatctaacaa aaatagtccg gttaaaaacc ccgtgtaact
     3361 ccatcgtgac cttcccttgt gagaattttt gtcagaaaat actcaggaaa tgttgtacat
     3421 ataatttaag cccggcaata atttctaaag tagctgcttg tgcagattat ggaatatgcc
     3481 tgaaagaatt tcttgaatac ctaattcttg aaaatttgag aacatagtaa catttgaaga
     3541 atgctttacc attccttatt attaattgcc gaagcatctt aaaaaaagca ccgactcggt
     3601 gccacttttt caagttgata acggactagc cttatcttaa cttgctatgc tgtttccagc
     3661 atagctctaa gacacaataa acattcaatg atgatcaatt agcctcattc atcttagtta
     3721 tatactcaat ggaaacggta aaagttctag cgatgggcga aaatattttt tcctatccat
     3781 aaagagagct ttaagttagt cctctttgac ctttttatta taatatttga tttgacctcg
     3841 ttggctttca atgacattct ttgagagaac aatgagtatc ttaaacgctc aaataaatat
     3901 tacctatcgc atctcttcat agtaaaataa atgttccaaa cagatccgtg tttgcgattg
     3961 catatttcaa ggcgtaatgt atttttcgga ggatggatgc tgacaatgaa accgtatccg
     4021 ttatcaccat ggagaggtgt tgaagaacga acaattaaga agaaagtttt ttgttttcgg
     4081 catttgatag gatcttacca ctcgaagcca atccagcgag acaaatagct tgaagttatt
     4141 tagcgcatcg ttaaattttt aacgactcta attggagctg ccagacaaat ttgatttgtc
     4201 cgtgcggtat gcatatgtac ctacttacgt ctgtcgtttt gtctccgttt accgggaggg
     4261 aaagtctgga aacatggaaa ctctatagtt gccaggtaga ccatctgcct ccgtcggctg
     4321 gctggattcc aatttgaata ttggctaatt ggaagagatg gaagtttttg aatggatgat
     4381 tgaataattg aagcgactcc gggtacctgt ttgtaagctc tgcaacagtg ccatagattc
     4441 gtgtcagtcc atcactagaa tcaaatcaac ttgtacttga tatataaatg gcttgggtta
     4501 atgtgcttcg gcaagacata tccgcggtta attaagcgat cgcgactcaa tactggaatc
     4561 ttgaaaatgt taggaacttt ataatgttac aattgctgtt atctcaagat tcattcatag
     4621 gatacagatt tctagctaaa attcgaaaat tctaaggaaa atccttgttg tcaaatcatt
     4681 ataaaattca catgcacata aagggaatac tgtaaagtta cataaccaac ataaatttca
     4741 tgatcgatta cgcgatgcat agcgggaatg tagtaatatt catattttca cataatttgt
     4801 cacgtgaata agtgacaaat ttggcatata tgcatgattt tattgatttc aagagtcact
     4861 ctgaatggaa ttttcttaac atgcagcatt actctgtact ttgaatagaa ttttcttaaa
     4921 gtagtaggta ctacatgttc gaaaaggttt ttattcatac caaaagtgta gtaaactttg
     4981 tggattttgt ttttgagtag atgctacatg tagtaaagtt caacgctttt tattcatacg
     5041 gcccagtgcc gcttatttcc aaggcattcc tcgccctaac cttatcttcg ccatgcacaa
     5101 caaactgctt cactgcaata ttaggaatcg atgaacaacc accaaaaaaa gcaccgactc
     5161 ggtgccactt tttcaagttg ataacggact agccttatct taacttgcta tgctgtttcc
     5221 agcatagctc taagacgtat atgttttagt ataataattt cactactctt gcctctgctc
     5281 ttatatagtc ttctacagcg aacgcggttc tagcgatgga tggacgtatc aactcgatgg
     5341 gcgatttcat tcgttcgcac cccaatcgga acggaacgct acccaggggc acacatagag
     5401 aagcgaaaaa aagtttagtt tttgtctacc tacaaaaagt caaacgtccc aacttattgc
     5461 tgatactcta ccctacacac ctatctgccg acgatcttca attataatac tgtatgtgct
     5521 tactgtaaag gatgactcat ctaacaccat tcctataacg atcaattaaa tattcaattt
     5581 gtttcaacta tgttggtcga ctcgacggag tcgcacagca cagccgcaca gtgcgttgaa
     5641 tgtatggaga tgtggacaga agtcataact aaaagaccag tctggattag tcaagtctag
     5701 tacacgacgg ccttgcagtt gaggtcgaaa tacgcgtatc tatcaaagga tacaaactct
     5761 agtggaatta aattatatag cactaaattc gggttccctt taacttactg tttatcgccg
     5821 aaaaatacca tgaaatttga tttttgtcta cgggaaaacg cactgtgagc cgctccgacc
     5881 acactcgagc agttgttaac agttttccca gtcgagcgaa gaataattag agtcttctgg
     5941 tggaatgtca acattcttga tgtgactctc tggtctgcag attcgatcga ctgtctggcg
     6001 cgctttgcat ctctcagagc ggtagtgaaa tgcaaatgaa gggttcaatt ccttgggaat
     6061 cgtcgtcgtc gtcgtcgtct aggacggcga gtgtggtata tgttttaatt tacgggattt
     6121 tcaactcgtc tcgtcacccg agcataattt atgccgaaag aaaggaatcc gactgactgg
     6181 tgacagatat gatgctaccg ttcccctgaa gacgattata atagtttcgt ttgtgatgaa
     6241 gatctcctag gagcccgggc gagctcgaat taaccattgt gggaaccgtg cgatcaaaca
     6301 aacgcgagat accggaagta ctgaaaaaca gtcgctccag gccagtggga acatcgatgt
     6361 tttgttttga cggacccctt actctcgtct catataaacc gaagccagct aagatggtat
     6421 acttattatc atcttgtgat gaggatgctt ctatcaacga aagtaccggt aaaccgcaaa
     6481 tggttatgta ttataatcaa actaaaggcg gagtggacac gctagaccaa atgtgttctg
     6541 tgatgacctg cagtaggaag acgaataggt ggcctatggc attattgtac ggaatgataa
     6601 acattgcctg cataaattct tttattatat acagccataa tgtcagtagc aagggagaaa
     6661 aggttcaaag tcgcaaaaaa tttatgagaa acctttacat gagcctgacg tcatcgttta
     6721 tgcgtaagcg tttagaagct cctactttga agagatattt gcgcgataat atctctaata
     6781 ttttgccaaa tgaagtgcct ggtacatcag atgacagtac tgaagagcca gtaatgaaaa
     6841 aacgtactta ctgtacttac tgcccctcta aaataaggcg aaaggcaaat gcatcgtgca
     6901 aaaaatgcaa aaaagttatt tgtcgagagc ataatattga tatgtgccaa agttgtttct
     6961 gactgactaa taagtataat ttgtttctat tatgtataag ttaagctaat tacttatttt
     7021 ataatacaac atgactgttt ttaaagtaca aaataagttt atttttgtaa aagagagaat
     7081 gtttaaaagt tttgttactt tatagaagaa attttgagtt tttgtttttt ttaataaata
     7141 aataaacata aataaattgt ttgttgaatt tattattagt atgtaagtgt aaatataata
     7201 aaacttaata tctattcaaa ttaataaata aacctcgata tacagaccga taaaacacat
     7261 gcgtcaattt tacgcatgat tatctttaac gtacgtcaca atatgattat ctttctaggg
     7321 ttaaataata gtttctaatt tttttattat tcagcctgct gtcgtgaata ccgtatatct
     7381 caacgctgtc tgtgagattg tcgtattcta gcctttttag tttttcgctc atcgacttga
     7441 tattgtccga cacattttcg tcgatttgcg ttttgatcaa agacttgagc agagacacgt
     7501 taatcaactg ttcaaattga tccatattaa cgatatcaac ccgatgcgta tatggtgcgt
     7561 aaaatatatt ttttaaccct cttatacttt gcactctgcg ttaatacgcg ttcgtgtaca
     7621 gacgtaatca tgttttcttt tttggataaa actcctactg agtttgacct catattagac
     7681 cctcacaagt tgcaaaacgt ggcatttttt accaatgaag aatttaaagt tattttaaaa
     7741 aatttcatca cagatttaaa gaagaaccaa aaattaaatt atttcaacag tttaatcgac
     7801 cagttaatca acgtgtacac agacgcgtcg gcaaaaaaca cgcagcccga cgtgttggct
     7861 aaaattatta aatcaacttg tgttatagtc acggatttgc cgtccaacgt gttcctcaaa
     7921 aagttgaaga ccaacaagtt tacggacact attaattatt tgattttgcc ccacttcatt
     7981 ttgtgggatc acaattttgt tatattttta aacaaagctt ggcactggcc gtcgttttac
     8041 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     8101 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     8161 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     8221 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8281 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     8341 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     8401 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     8461 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8521 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8581 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8641 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8701 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8761 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8821 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8881 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8941 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9001 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9061 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9121 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9181 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9241 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9301 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9361 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9421 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9481 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9541 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9601 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9661 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9721 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9781 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9841 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9901 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9961 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10021 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10081 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10141 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10201 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10261 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10321 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10381 cgaccgagcg cagcgagtca gtgagcgagg a
//
