LOCUS       sequence-371154-        8606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Flag-tagged human TEX14 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8606)
  TITLE     Flag-tagged mammalian expression constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Flag-hTEX14
FEATURES             Location/Qualifiers
     source          1..8606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        845..863
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     845..862
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             901..924
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    5468..5602
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5492..5511)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5546..5565
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5715..5735)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5715..5733)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5754..5770)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5754..5770)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5767..5789)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5778..5794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5802..5808,5809..5826,5827..5832))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5847..5868
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5985..6002)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6156..6744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6236..6255)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6915..7706,7707..7775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7538..7557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7776..7880)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7906..8361)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8043..8064)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8255..8274
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(8432..8606,1..810)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 acctggcact gtgccaactg gggaggggtc tacttggcac ggtgccaagt ttgaggaggg
       61 gtcttggccc tgtgccaagt ccgccatatt gaattggcat ggtgccaata atggcggcca
      121 tattggctat atgccaggat caatatatag gcaatatcca atatggccct atgccaatat
      181 ggctattggc caggttcaat actatgtatt ggccctatgc catatagtat tccatatatg
      241 ggttttccta ttgacgtaga tagcccctcc caatgggcgg tcccatatac catatatggg
      301 gcttcctaat accgcccata gccactcccc cattgacgtc aatggtctct atatatggtc
      361 tttcctattg acgtcatatg ggcggtccta ttgacgtata tggcgcctcc cccattgacg
      421 tcaattacgg taaatggccc gcctggctca atgcccattg acgtcaatag gaccacccac
      481 cattgacgtc aatgggatgg ctcattgccc attcatatcc gttctcacgc cccctattga
      541 cgtcaatgac ggtaaatggc ccacttggca gtacatcaat atctattaat agtaacttgg
      601 caagtacatt actattggaa gtacgccagg gtacattggc agtactccca ttgacgtcaa
      661 tggcggtaaa tggcccgcga tggctgccaa gtacatcccc attgacgtca atggggaggg
      721 gcaatgacgc aaatgggcgt tccattgacg taaatgggcg gtaggcgtgc ctaatgggag
      781 gtctatataa gcaatgctcg tttagggaac cgccattctg cctggggacg tcggagcaag
      841 cttgatttag gtgacactat agaatacaag ctacttgttc tttttgcagg atccaccatg
      901 gattacaagg atgacgacga taagatctcg aattccatgt ctcgggctgt tcgtcttcca
      961 gtcccctgtc ctgttcaact tggtacctta agaaatgact ccctggaagc tcagcttcat
     1021 gagtatgtca aacaagggaa ctatgtgaaa gtgaagaaaa ttcttaagaa aggaatttat
     1081 gttgatgcag ttaactcctt gggccaaaca gcactttttg ttgcggcgtt attgggcctt
     1141 aggaaattcg ttgatgttct ggtggattat ggatcagatc caaatcaccg ctgctttgat
     1201 gggagcaccc ctgtccatgc agcagcattt tcgggcaatc agtggatcct tagcaaactg
     1261 ctggatgcag gaggtgacct gcgactccac gatgagaggg gtcaaaaccc gaagacttgg
     1321 gctttgacag caggaaagga gcgtagcacc cagatagtgg agttcatgca gcgctgtgcc
     1381 tcacacatgc aggccatcat ccagggcttc tcttacgacc tcctgaagaa gatagactcc
     1441 ccgcagcggc ttgtctacag cccgtcctgg tgtgggggcc tcgtgcaggg aaaccctaat
     1501 ggctctccta accgactgct taaagctgga gtcatttctg ctcaaaatat ctacagcttt
     1561 ggttttggga aggctatgcc ttggtttcag ttttatctta ctggggcgac acagatggcc
     1621 tatctaggat ctcttccggt cattggagaa aaggaagtga ttcaagctga tgatgagccc
     1681 accttctctt tcttcagcgg cccctacatg gtcatgacca acctagtgtg gaatgggagc
     1741 agggtcacag tgaaagagct gaatctcccc acccacccac actgcagcag gctgcggctg
     1801 gccgacttgt taattgccga gcaggaacac agcagcaagc tgcggcaccc ctacttgcta
     1861 cagttgatgg ctgtgtgtct ctcccaggac ctagagaaaa cccgccttgt gtacgagcgc
     1921 atcactatcg gcacattgtt cagtgtcctt catgaacgac ggtcccagtt cccagtgctg
     1981 cacatggagg tgattgtgca cctgctgctc cagatatctg atgccctgag atacctgcat
     2041 ttccaggggt ttatccaccg ctccctcagc tcctatgctg tccatatcat ctcccctggt
     2101 gaagcgaggc tgaccaacct ggagtacatg ttggaaagcg aggacagagg tgtacagagg
     2161 gacctgactc gagtgcccct tcctacgcag ctatacaact gggccgcacc agaagtgatc
     2221 ttacagaagg cagccacagt gaaatcagac atctacagct tttctatgat catgcaggag
     2281 attttaacag atgacatacc ctggaagggc ttagatggct cagttgttaa aaaagccgta
     2341 gtctcgggga attatttaga agctgatgtc aggcttccga aaccttacta tgatattgtt
     2401 aagtcaggca tccacgtcaa gcagaaagac cgaactatga accttcaaga tatccggtat
     2461 attctgaaga atgacttaaa ggattttact ggagcccaga gaactcaacc aaccgagagc
     2521 cccagagtgc agagatacgg actccatccc gatgtcaatg tctatctagg actgacttca
     2581 gaacacccca gagagacacc tgacatggaa atcatagaac taaaggaaat gggcagtcaa
     2641 cctcattcac caagggttca ctctttattc actgagggga cactagatcc tcaggcccca
     2701 gatccatgtc tgatggccag ggagactcag aatcaagatg ctccttgccc tgctccattt
     2761 atggcagaag aggccagcag ccccagcaca ggtcagccaa gcctctgcag tttcgaaatc
     2821 aacgagatct actcaggctg cttgattttg gaagatgaca tagaagagcc tccaggagct
     2881 gcttcatctt tggaggcaga cggacctaac caggtagatg aactgaaatc catggaagaa
     2941 gagctggata agatggagag agaggcgtgt tgttttggca gtgaggatga gagctcttca
     3001 aaagctgaga cagagtactc ttttgatgac tgggactggc aaaacggttc actcagttca
     3061 ctcagccttc ctgagtcaac cagagaagcc aagagcaatt tgaacaacat gtccacgact
     3121 gaggagtatc tcatcagtaa gtgtgtgctg gatctaaaga ttatgcagac aataatgcac
     3181 gagaatgatg ataggctgag gaatatcgag cagatattag atgaagtcga gatgaaacag
     3241 aaggaacagg aagagcgcat gtctttatgg gccacttcaa gagagtttac aaatgcctac
     3301 aagttacctc tggccgtggg ccctccatct ttaaactata ttcctcctgt cctacagctt
     3361 tcagggggtc agaagccaga caccagtggc aactacccaa ccctaccaag atttccaaga
     3421 atgctgccga ctctttgtga ccctggaaaa cagaacacag atgaacaatt tcagtgcact
     3481 caaggagcca aggacagttt ggaaacaagc aggatccaaa ataccagtag ccagggaaga
     3541 cctagagagt ccactgccca agccaaagcc acacagttta atagtgcact cttcactctg
     3601 tcaagccacc ggcagggacc ttctgcatca cccagctgtc actgggactc taccaggatg
     3661 agtgtggaac ctgtttcttc tgaaatctat aatgcagagt ccagaaataa agatgatgga
     3721 aaggtacact taaaatggaa aatggaggtg aaagaaatgg caaagaaagc agctactgga
     3781 cagctcacag tacctccttg gcatcctcag agtagtctga ctttagagag cgaggctgaa
     3841 aatgagcccg acgccctgct gcagcccccc attaggagcc cagaaaacac ggattggcag
     3901 cgagttattg agtatcatag ggaaaatgat gagcccagag gaaatggcaa gtttgacaag
     3961 acgggcaaca atgactgtga cagtgaccag catggcagac agcccaggct tggaagcttc
     4021 accagtatca ggcacccatc tcccagacaa aaggagcaac cagagcatag tgaagccttc
     4081 caagcaagtt ctgacacatt ggtggctgta gagaaatctt acagtcatca gtccatgcaa
     4141 tcaacttgtt caccagagtc ttctgaggat ataacagatg aatttttaac tccagacggt
     4201 gaatattttt actcctcgac tgctcaagaa aacttagctc tagagacctc gagtcccata
     4261 gaagaggact ttgaaggaat acaaggtgca tttgcccaac ctcaagtctc tggtgaggaa
     4321 aagttccaaa tgagaaaaat tcttggaaag aatgctgaga ttttgcccag gtctcaattt
     4381 caacctgtac gaagtactga agatgaacaa gaagagacat caaaggagtc accaaaggaa
     4441 ctgaaagaga aagacatatc attgacggat attcaagacc tgtctagtat ctcctatgaa
     4501 ccagacagct cttttaagga agcttcatgc aaaacaccca aaataaacca tgcacctacc
     4561 agtgtcagca ctccactcag cccagggtcc gtttcttcag ctgccagtca gtataaagac
     4621 tgccttgaaa gtatcacatt tcaggttaag acagagtttg cctcttgctg gaacagtcaa
     4681 gaatttattc aaactttgtc tgatgacttt ataagtgtcc gagagagagc aaagaaactg
     4741 gattctctcc ttacttcctc tgaaactccc ccttcaagac tgactggtct taaaagattg
     4801 tcttcattta ttggggctgg atcccccagc cttgttaagg catgtgactc atcaccaccc
     4861 catgccaccc agagaaggag cctgcctaaa gtagaagcct tctcacagca tcacattgat
     4921 gagctgccac caccatctca ggagctactt gatgacattg agctcttgaa acagcagcag
     4981 ggctcatcca cggtgttgca tgagaacaca gcaagtgatg gaggaggcac tgcaaatgat
     5041 caaaggcact tagaagaaca agaaactgac agtaaaaaag aagatagtag tatgcctttg
     5101 tccaaagaaa ctgaagatct tggagaggac acagagagag ctcactctac tctggatgag
     5161 gacctggaaa gatggctgca gccacctgag gagagcgtgg agctacaaga ccttcccaag
     5221 ggctctgaaa gggagacaaa tatcaaagat caaaaagttg gtgaagagaa aagaaaaagg
     5281 gaagatagca ttacaccaga gagaaggaaa tcagagggtg ttctagggac ttctgaagaa
     5341 gatgaactaa aatcctgttt ttggaagcga ctaggttggt ccgaatcatc caggataatc
     5401 gtgctggatc agagtgactt gtcagactga gtcgagcctc tagaactata gtgagtcgta
     5461 ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     5521 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     5581 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     5641 gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta
     5701 cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     5761 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccgggagc
     5821 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5881 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     5941 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6001 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6061 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6121 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6181 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6241 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6301 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6361 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6421 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6481 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6541 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6601 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6661 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6721 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6781 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6841 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6901 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7021 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7201 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7501 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7861 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     7921 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     7981 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     8041 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     8101 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     8161 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     8221 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     8281 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     8341 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
     8401 gatcggtgcg ggcctcttcg ctattacgcc agtcgaccgc caattcaata tggcgtatat
     8461 ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat
     8521 ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata tggcggactt
     8581 ggcaccatgc caattcaata tggcgg
//
