LOCUS       sequence-371157-        5507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human CEP55 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5507)
  TITLE     Flag-tagged mammalian expression constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Flag-hCEP55
FEATURES             Location/Qualifiers
     source          1..5507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        105..1089
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1124..1142
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1124..1141
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1180..1203
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2648..2782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2672..2691)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2726..2745
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2895..2915)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2895..2913)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2934..2950)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2934..2950)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2947..2969)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2958..2974
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2982..2988,2989..3006,3007..3012))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3027..3048
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3165..3182)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3336..3924)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3416..3435)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4095..4886,4887..4955))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4718..4737
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4956..5060)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(5086..5507,1..34))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5223..5244)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5435..5454
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
       61 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagtcgac cgccaattca
      121 atatggcgta tatggactca tgccaattca atatggtgga tctggacctg tgccaattca
      181 atatggcgta tatggactcg tgccaattca atatggtgga tctggacccc agccaattca
      241 atatggcgga cttggcacca tgccaattca atatggcgga cctggcactg tgccaactgg
      301 ggaggggtct acttggcacg gtgccaagtt tgaggagggg tcttggccct gtgccaagtc
      361 cgccatattg aattggcatg gtgccaataa tggcggccat attggctata tgccaggatc
      421 aatatatagg caatatccaa tatggcccta tgccaatatg gctattggcc aggttcaata
      481 ctatgtattg gccctatgcc atatagtatt ccatatatgg gttttcctat tgacgtagat
      541 agcccctccc aatgggcggt cccatatacc atatatgggg cttcctaata ccgcccatag
      601 ccactccccc attgacgtca atggtctcta tatatggtct ttcctattga cgtcatatgg
      661 gcggtcctat tgacgtatat ggcgcctccc ccattgacgt caattacggt aaatggcccg
      721 cctggctcaa tgcccattga cgtcaatagg accacccacc attgacgtca atgggatggc
      781 tcattgccca ttcatatccg ttctcacgcc ccctattgac gtcaatgacg gtaaatggcc
      841 cacttggcag tacatcaata tctattaata gtaacttggc aagtacatta ctattggaag
      901 tacgccaggg tacattggca gtactcccat tgacgtcaat ggcggtaaat ggcccgcgat
      961 ggctgccaag tacatcccca ttgacgtcaa tggggagggg caatgacgca aatgggcgtt
     1021 ccattgacgt aaatgggcgg taggcgtgcc taatgggagg tctatataag caatgctcgt
     1081 ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg tgacactata
     1141 gaatacaagc tacttgttct ttttgcagga tccaccatgg attacaagga tgacgacgat
     1201 aagatctcga attccatgtc ttccagaagt accaaagatt taattaaaag taagtgggga
     1261 tcgaagccta gtaactccaa atccgaaact acattagaaa aattaaaggg agaaattgca
     1321 cacttaaaga catcagtgga tgaaatcaca agtgggaaag gaaagctgac tgataaagag
     1381 agacacagac ttttggagaa aattcgagtc cttgaggctg agaaggagaa gaatgcttat
     1441 caactcacag agaaggacaa agaaatacag cgactgagag accaactgaa ggccagatat
     1501 agtactaccg cattgcttga acagctggaa gagacaacga gagaaggaga aaggagggag
     1561 caggtgttga aagccttatc tgaagagaaa gacgtattga aacaacagtt gtctgctgca
     1621 acctcacgaa ttgctgaact tgaaagcaaa accaatacac tccgtttatc acagactgtg
     1681 gctccaaact gcttcaactc atcaataaat aatattcatg aaatggaaat acagctgaaa
     1741 gatgctctgg agaaaaatca gcagtggctc gtgtatgatc agcagcggga agtctatgta
     1801 aaaggacttt tagcaaagat ctttgagttg gaaaagaaaa cggaaacagc tgctcattca
     1861 ctcccacagc agacaaaaaa gcctgaatca gaaggttatc ttcaagaaga gaagcagaaa
     1921 tgttacaacg atctcttggc aagtgcaaaa aaagatcttg aggttgaacg acaaaccata
     1981 actcagctga gttttgaact gagtgaattt cgaagaaaat atgaagaaac ccaaaaagaa
     2041 gttcacaatt taaatcagct gttgtattca caaagaaggg cagatgtgca acatctggaa
     2101 gatgataggc ataaaacaga gaagatacaa aaactcaggg aagagaatga tattgctagg
     2161 ggaaaacttg aagaagagaa gaagagatcc gaagagctct tatctcaggt ccagtttctt
     2221 tacacatctc tgctaaagca gcaagaagaa caaacaaggg tagctctgtt ggaacaacag
     2281 atgcaggcat gtactttaga ctttgaaaat gaaaaactcg accgtcaaca tgtgcagcat
     2341 caattgcttg taattcttaa ggagctccga aaagcaagaa atcaaataac acagttggaa
     2401 tccttgaaac agcttcatga gtttgccatc acagagccat tagtcacttt ccaaggagag
     2461 actgaaaaca gagaaaaagt tgccgcctca ccaaaaagtc ccactgctgc actcaatgaa
     2521 agcctggtgg aatgtcccaa gtgcaatata cagtatccag ccactgagca tcgcgatctg
     2581 cttgtccatg tggaatactg ttcaaagtag ctcgagcctc tagaactata gtgagtcgta
     2641 ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     2701 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     2761 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     2821 gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta
     2881 cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     2941 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccgggagc
     3001 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     3061 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     3121 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     3181 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3241 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3301 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3361 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3421 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3481 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3541 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3601 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3661 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3721 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3781 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3841 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3901 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3961 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     4021 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     4081 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4141 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4201 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4261 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4321 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4381 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4441 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4501 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4561 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4621 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4681 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4741 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4801 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4861 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4921 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4981 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5041 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     5101 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     5161 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     5221 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     5281 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     5341 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     5401 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     5461 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccaca
//
