LOCUS       sequence-371177-        7299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding SauriABE8e driven by the CMV promoter for plasmid 
            transfection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Davis JR, Wang X, Witte IP, Huang TP, Levy JM, Raguram A, Banskota 
            S, Seidah NG, Musunuru K, Liu DR
  TITLE     Efficient in vivo base editing via single adeno-associated viruses 
            with size-optimized genomes encoding compact adenine base editors.
  JOURNAL   Nat Biomed Eng. 2022 Jul 28. pii: 10.1038/s41551-022-00911-4. doi: 
            10.1038/s41551-022-00911-4.
  PUBMED    35902773
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng. 2022 Jul 28. pii: 10.1038/s41551-022-00911-4. doi: 
            10.1038/s41551-022-00911-4."
COMMENT     Sequence Label: CMV-SauriABE8e
FEATURES             Location/Qualifiers
     source          1..7299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(273..1064,1065..1133))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     896..915
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1134..1238)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1317..1336)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1508..1887
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1888..2091
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2042..2062
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2133..2152
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2133..2151
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2201..2221
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6041..6061
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6070..6087
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6110..6127)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6116..6340
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6411..6427)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6411..6427)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6424..6446)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6435..6451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6459..6465,6466..6483,6484..6489))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6504..6525
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6642..6659)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(6813..7299,1..102))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6893..6912)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
       61 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      121 tctacggggt ctgacactca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      181 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      241 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      301 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
      361 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
      421 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
      481 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
      541 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
      601 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
      661 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
      721 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
      781 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      841 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      901 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      961 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1021 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1081 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1141 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1201 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1261 cctgacgtcg acggatcggg agatcgatct cccgatcccc tagggtcgac tctcagtaca
     1321 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     1381 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     1441 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     1501 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1561 catagcccat atattgagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1621 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1681 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1741 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1801 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1861 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     1921 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     1981 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2041 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
     2101 aaccgtcaga tccgctagag atccgcggcc gctaatacga ctcactatag ggagagccgc
     2161 caccatgaaa cggacagccg acggaagcga gttcgagtca ccaaagaaga agcggaaagt
     2221 ctctgaggtg gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag
     2281 ggcacgggat gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat
     2341 cggcgagggc tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat
     2401 ggccctgaga cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta
     2461 cgtgacattc gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg
     2521 cgtggtgttt ggcgtgagga actcaaaaag aggcgccgca ggctccctga tgaacgtgct
     2581 gaactacccc ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg
     2641 tgccgccctg ctgtgcgatt tctatcggat gcctagacag gtgttcaatg ctcagaagaa
     2701 ggcccagagc tccatcaact ccggaggatc tagcggaggc tcctctggct ctgagacacc
     2761 tggcacaagc gagagcgcaa cacctgaaag cagcgggggc agcagcgggg ggtcaatgca
     2821 ggagaaccag cagaagcaaa attacatcct gggcctggcc atcggcatca ccagcgtggg
     2881 ctatggcctg atcgacagca agaccagaga agtgattgac gccggcgtgc ggctattccc
     2941 agaggccgac tctgaaaaca acagcaatag aagatctaag cggggcgccc ggagactgaa
     3001 aagacgaaga atccacagac tgaacagagt gaaagacctc ctggctgact accagatgat
     3061 cgacttaaac aacgtgccca agtctaccga cccctacacc atccgggtga agggactgcg
     3121 ggaacctctg accaaggaag agtttgccat cgctctgctg catatcgcca agagaagagg
     3181 cctgcacaac atctccgtga gcatgggcga tgaggaacag gacaacgagc tgtccaccaa
     3241 gcagcagctg cagaagaacg ctcagcagct gcaggacaaa tacgtgtgcg agctgcaact
     3301 ggaaagactg accaacatca acaaggtcag aggcgagaag aaccggttca agaccgaaga
     3361 tttcgtgaag gaagtgaagc agctgtgcga gacccagcgg cagtaccaca acatcgacga
     3421 tcagttcatc cagcagtaca tcgacctggt gagcacccgg agagaatact tcgagggccc
     3481 tggcaacggc tctccatatg gctgggatgg agatctgctg aagtggtatg agaagctgat
     3541 gggcagatgc acctacttcc ctgaggagct gagaagcgtg aagtacgcct acagcgccga
     3601 tctgtttaac gccctgaacg atctgaacaa cctggttgtg acccgcgacg acaaccctaa
     3661 gctggaatac tacgagaaat accacattat cgagaacgtg ttcaagcaga agaaaaatcc
     3721 aactctgaag caaatcgcca aagagatcgg cgtgcaggat tacgacatca gaggatacag
     3781 aattaccaag tccggtaagc ctcagttcac cagcttcaaa ctctaccacg acctgaaaaa
     3841 catctttgaa caggccaaat acctggaaga tgtggagatg ctggatgaga tagctaaaat
     3901 cctgacaatc taccaagacg agatcagcat caagaaagcc ctggaccagc tgcctgagct
     3961 gctgaccgag agcgaaaaaa gccagatcgc tcagctgacc ggctacaccg gtacacatag
     4021 actgtctctg aagtgcatcc acatcgtgat cgacgagctg tgggagagcc ccgaaaacca
     4081 gatggaaatc ttcaccagac tgaacctcaa gccaaagaag gtggaaatga gcgagatcga
     4141 cagcatccct accacactgg tggatgagtt catcctgagc cctgtggtga agcgggcctt
     4201 catccagtcc atcaaggtga tcaacgctgt gatcaacaga ttcggcctgc ccgaggacat
     4261 cattatcgag ctggccagag agaagaacag caaggacaga aggaagttca tcaacaagct
     4321 gcagaaacag aatgaggcca cccggaaaaa aatcgagcag ctgctggcca agtacggcaa
     4381 taccaatgcc aagtacatga tcgaaaagat caagctgcat gacatgcagg agggcaagtg
     4441 tctgtacagc ctggaagcta tccccctgga agacctgctg tctaatccta cacactacga
     4501 ggtggaccac atcatcccta gaagcgtgtc cttcgacaac agcctgaaca acaaggttct
     4561 ggtgaagcaa agcgagaaca gcaagaaggg caataggacc ccttaccagt acctgagcag
     4621 caacgagtcc aagatctctt acaaccagtt caagcagcac attctgaacc tgtctaaggc
     4681 caaagataga atcagcaaga agaaacgaga tatgctgctg gaagaacggg acatcaacaa
     4741 attcgaggtg cagaaggaat tcatcaacag aaaccttgtg gacacccggt acgccactcg
     4801 ggagctgagc aacctgctga agacctactt cagcacacac gactacgccg tgaaagtgaa
     4861 gaccatcaac ggcggcttca caaaccacct gaggaaggtg tgggacttca agaagcaccg
     4921 gaaccacggc tacaagcacc acgccgagga tgccctggtc atcgccaacg ccgactttct
     4981 gttcaaaacc cacaaggccc tgagacggac agacaagatc ctggaacagc ctggactgga
     5041 agtcaacgac accaccgtga aggtggacac agaggagaag taccaggagt tattcgagac
     5101 accgaaacaa gtgaagaaca tcaagcagtt tagagatttc aagtattctc acagagttga
     5161 caagaagccc aaccggcagc tgatcaatga taccctgtac agtaccagag agatcgatgg
     5221 cgaaacctac gtggtccaaa cactgaaaga cctgtacgcc aaggacaatg aaaaggtgaa
     5281 aaagctcttt acagaacggc ctcaaaagat actgatgtac cagcacgatc ctaagacctt
     5341 tgagaaactg atgaccattc tgaatcagta cgctgaggca aagaatcctc tggccgctta
     5401 ttacgaggat aagggcgaat acgtgaccaa gtacgccaag aagggcaacg gccctgccat
     5461 ccacaagatc aaatacatcg acaagaaact gggcagctac ctggacgtga gtaacaaata
     5521 tcctgagaca cagaacaagc tggtgaaact gtctctgaag agctttagat tcgacatcta
     5581 caaatgtgaa cagggctaca agatggtgtc cattggctac ctcgacgtac tgaagaagga
     5641 caactactac tacatcccca aagataagta cgaggccgag aagcagaaaa agaagatcaa
     5701 ggaaagcgac ctcttcgtgg gcagcttcta ctacaacgac ctgatcatgt acgaggacga
     5761 actcttccgg gtgatcggag tgaactccga tatcaacaac ctggttgagc tgaatatggt
     5821 cgacatcacc tacaaggatt tctgcgaggt gaacaacgtg acaggcgaga agagaatcaa
     5881 gaaaaccatc ggaaagagag tggtgctgat cgagaagtat acgaccgaca tcctgggaaa
     5941 tctgtataaa acgcccctgc ctaagaagcc ccagctcatt ttcaagagag gcgagctgtc
     6001 tggcggctca aaaagaaccg ccgacggcag cgaattcgag cccaagaaga agaggaaagt
     6061 ctaaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
     6121 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     6181 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     6241 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     6301 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     6361 agctggggct cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6421 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6481 aagtgtaaag cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6541 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6601 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6661 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6721 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6781 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6841 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6901 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6961 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7021 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7081 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7141 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7201 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7261 tttggtatct gcgctctgct gaagccagtt accttcgga
//
