LOCUS       sequence-371197-        6390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses paxillin fused to blue fluorescent protein Electra2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, 
            Jia JM, Koster RW, Namikawa K, Piatkevich KD
  TITLE     Dual-expression system for blue fluorescent protein optimization.
  JOURNAL   Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0.
  PUBMED    35715437
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-06-17"; volume: "12(1):10190. doi"; pages: " 
            10.1038/s41598-022-13214-0"
COMMENT     Sequence Label: pPaxillin-Electra2
FEATURES             Location/Qualifiers
     source          1..6390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    2277..2329
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      2337..2346
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3178..3299
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3215..3234)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3269..3288
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3306..3761)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3443..3464)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3655..3674
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3788..3892
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3894..4251
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3894..4085
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3917..3937)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4102..4237
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4286..5080
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4286..5080
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4340..4359)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4950..4969
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5268..5287)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5312..5359
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5688..6276
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6177..6196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcacca
      601 tggacgacct cgatgcctta ctggcagacc tggaatccac cacctcgcat atctctaagc
      661 gaccggtgtt tctaacagag gaaacgcctt actcctaccc aactggaaac catacgtacc
      721 aggagattgc tgtgccaccg ccggtgcccc cgccgccttc cagtgaggcc ctaaatggca
      781 ctgtgattga ccccttagac cagtggcaac ccagcgtgtc cagatacggc caccagcaac
      841 ctcagtccca gtctccgata tacagctcta gtgccaaaag ctccagtgcc tctgttccta
      901 gagacgggct cagctcccct tctccccgtg ccagtgagga ggaacacgtg tacagcttcc
      961 caaacaagca gaagtctgca gaaccatctc ccacaatgac cagcacctcc ctgggcagca
     1021 acctctcgga actggacagg cttctcctgg aactgaatgc tgttcaacat aatcccccca
     1081 gtggcttctc agcagatgaa gtcagcagaa gcccatcact gcccaatgtg accggacctc
     1141 actatgtcat cccagagagc agcagctctg caggagggaa ggctgcaccc cctacaaaag
     1201 aaaaaccaaa gcggaatggt gggcgtggga ttgaagatgt gcgtcccagt gtggagagcc
     1261 tgctggatga gctggagagc tctgtgccaa gcccagtccc tgcaatcact gtgagccaag
     1321 gggaggtgag cagcccccag cgtgtcaacg ccagtcagca gcagacccgt atctctgctt
     1381 cttcagctac acgagaactg gatgagctga tggcgtccct ctctgacttt aagttcatgg
     1441 cacaggggaa agccggcggc agcagctccc caccatccac caccccaaag cctggcagtc
     1501 agctggacac catgctggga agtctgcagt ctgacctgaa caaactgggg gtagcgacgg
     1561 tcgccaaggg tgtgtgcgga gcctgcaaga agccgattgc tgggcaggta gttacagcca
     1621 tggggaagac ctggcaccct gagcacttcg tctgcaccca ctgccaggag gagattggat
     1681 cacggaactt ctttgagcgg gatgggcagc cctactgtga gaaggactat cacaacctct
     1741 tctctcctcg ctgctactac tgcaatgggc caatccttga taaagtggtg acggctttgg
     1801 acaggacgtg gcaccctgaa cactttttct gtgcccagtg tggagttttc tttggacctg
     1861 aaggatttca tgagaaggat ggcaaagcct attgccgcaa ggactacttt gacatgtttg
     1921 ctcccaagtg tggaggctgt gcccgggcta tcctggaaaa ttacatttct gccttaaaca
     1981 ccctgtggca ccctgagtgc tttgtctgtc gggaatgttt cacaccgttc atcaacggca
     2041 gcttcttcga gcacgacggg cagccctact gcgaggtgca ttaccacgag cgccgcggct
     2101 cgctgtgctc tggctgccag aagcccatca cgggacgctg catcactgcc atgggcaaga
     2161 aatttcaccc cgaacacttt gtctgtgcct tctgcctcaa gcagctcaac aaaggaacct
     2221 tcaaagaaca gaacgacaag ccctactgtc agaactgctt tctcaaactc ttctgtctcg
     2281 agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
     2341 ccatggtgtc taagggcgaa gagctgatcg aggaaaatat gcgtatgaag gtggtcatgg
     2401 aaggttcggt caacggccac caattcaaat gcacaggtga aggagaaggc agaccgtacg
     2461 agggagtgca aaccatgagg atcaaagtca tcgagggagg acccctgcca tttgcctttg
     2521 acattcttgc cacgtcgttc ctgttcggca gcaagacctt tatcaagtac ccggccgaca
     2581 tccctgattt cttcgaacag tcctttcctg agggctttac ttgggaaaga gttacgagat
     2641 acgaagatgg tggagtagtc accgtcacgc aggacaccag ccttgaggat ggtgggctcg
     2701 tctacaacgt caaggtcaga ggggtaaact ttcactccaa gggtcccgtg atgcagaaga
     2761 agaccgaggg ttgggagcct ttcacagaga tgatgtatcc agcagatggt ggtctgagag
     2821 gatataccga catcgcactg aaagttgatg gtggtggcca tctgcacgcc aacatcgtga
     2881 caacttacag gtcaaaaaag accgtcggga acatcaagat gcccggtgtc catgccgttg
     2941 attaccgcct ggaaaggatc gaagagagtg acaatgaaac ctacgtagtg ctaagagaag
     3001 tggcagttgc caaatacagc aaccttggtg gtggcatgga cgagctgttc aagtaagcgg
     3061 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     3121 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     3181 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3241 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     3301 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     3361 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     3421 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     3481 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     3541 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     3601 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     3661 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     3721 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     3781 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3841 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     3901 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3961 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4021 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4081 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4141 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4201 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     4261 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4321 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4381 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     4441 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     4501 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4561 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4621 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4681 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4741 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4801 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4861 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4921 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4981 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5041 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     5101 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     5161 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     5221 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     5281 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     5341 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     5401 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     5461 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     5521 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5581 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5641 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5701 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5761 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5821 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5881 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5941 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6001 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6061 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6121 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6181 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6241 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6301 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6361 tctgtggata accgtattac cgccatgcat
//
