LOCUS       sequence-371199-       13329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High-yield production of coenzyme F420 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13329)
  AUTHORS   Last D, Hasan M, Rothenburger L, Braga D, Lackner G
  TITLE     High-yield production of coenzyme F420 in Escherichia coli by 
            fluorescence-based screening of multi-dimensional gene expression 
            space.
  JOURNAL   Metab Eng. 2022 Jul 18. pii: S1096-7176(22)00093-3. doi: 
            10.1016/j.ymben.2022.07.006.
  PUBMED    35863619
REFERENCE   2  (bases 1 to 13329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2022 Jul 18. pii: S1096-7176(22)00093-3. doi: 
            10.1016/j.ymben.2022.07.006."
COMMENT     Sequence Label: pDL52 (clone76)
FEATURES             Location/Qualifiers
     source          1..13329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    5034..5058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5183..5207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6890..6898
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      8360..8406
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      8443..8898
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8530..8549)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8740..8761
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8990..9805)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8990..9805)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9713..9732
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9797..9816
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9927..10515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10416..10435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10669..10686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10701..10840
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10766..10785)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10926..10948
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10942..11133)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(11942..13024)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     12986..13005
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(13025..13102)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     13308..13327
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtcccta ttatatcagc
       61 gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct atcccaggtt
      121 tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct gcgaattgcc
      181 ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat cggcacgttg
      241 agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc gtgcgggcca
      301 gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg tgcattgccg
      361 ttagtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc gtgagtttgc
      421 tgcgggacgt cgattcggct gcgccatttg gcaaagttga tcattgctgc gtgtgcgcct
      481 gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat cgaacgaaaa
      541 cgccagcgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc ctggcgcagg
      601 cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg cacaaaggta
      661 agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt atcatccagg
      721 tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcatcgg tacgtcctta
      781 tttaagccct attatttgtt cagcaaatgg cccattttct cggctttggt atcgagataa
      841 tgttcgttat tggggttacg acctacaatc aatggtacgc gttcaacaat attaatccct
      901 gcttcggtca gaatttcgac ttttttcggg ttattggtta acaagcggac ttcattgacg
      961 ccaaggagtt tgaacatatc agcgcaaaga gtgaagtcgc gctcatcagc ggcgaagcct
     1021 aactggtggt tagcctctac ggtatcgtaa ccttgatcct gcagtgcgta agcgcggatt
     1081 ttattcagca gaccaatgtt acgaccttcc tgacggtgat acagcaaaat accacggcct
     1141 tcctcggcaa tttgcgtcaa tgccgcttcg agctggaagc cacaatcgca gcgcaagctg
     1201 aacagggcgt caccggtcag acattcggaa tggacgcgcg caagtaccgg ggtatgcccg
     1261 gaaatatcgc catagactag cgcgacatga tcgtgtccgg ttgccagttc ttcaaatccc
     1321 accatcagga aatcgcccca tggggttggc agtttggctt ctgccacacg tttaagctgc
     1381 atcaacgtac cccctgaaaa cacctattat gcacctacta aatgcaggca aacatccggg
     1441 cctacatgac gtatctcttt gaatttaaat tggggggcgt cggctaattt ctcaagccct
     1501 ggcagcgagc ataatccacg ggcgtcgctg cctaatagtt taggtgcgat atagacaatc
     1561 agctcatcga ctaaacccgc ctgcagcaat gcgccagcga gcgttggccc cgcttccacc
     1621 cagatgctgt taatttgctg tttacccagt tgcatcatca gtacaaccag atccagatga
     1681 cctttatgcg ctggaatcag caaggtacgc accgtttccg gccactcacg agaatcttcc
     1741 tgcgtacgcg cgaaccaggt ttcgccgggc tgctgcacaa tgcgatgtac cggcgtcacg
     1801 cgattttggc tatcaatcac aatacgtatc ggctgacgga gattttgttg cggatagagc
     1861 gcctgagttt gttcatccag ttcagaccaa cgcaccgtta aggcaggatc atccgccagc
     1921 accgtggcgc tgctggttaa aatggcatga ctttgcgcgc gcagtagttg tacatcacgc
     1981 cgcgcctggg gcgaagtgat ccactggctt tcgccgctcg ccatcgccgt gcgaccatca
     2041 agcgatgcgc caagttttaa ctgaatataa ggaaagccgg tgcgcatccg cttgagaaag
     2101 cctttattca attgctcggc ttcactcatc atcaggccgt ggctgacgtc aatgccagcc
     2161 tgttgcagac ggtaaagtcc acgcccagcg acctgcgggt taggatcttg catcgaggca
     2221 accacgcgcg ctacgccagc ggcgatgagt gcgtcacagc acggtggcgt acgaccatga
     2281 tggctacagg gttcgagtgt gacataggcg gtcgcacctt tggctttttc acccgccata
     2341 cgcaacgcgt gtacttcggc atgtggttca cccgcacgtt ggtggtaacc ttcaccgaca
     2401 atttcgccat ctttgacaat gacgcacccg acattcgggt tgggatgcgt ggtaaaacgt
     2461 cctcgttgcg ccagctttag cgcccgcgcc atgtaatact cgtcctgcat ccctgaacct
     2521 cctttgatga cctattactg ttcggcttcg ctcagcacct gatgaccatg accgcgaacc
     2581 aggcgacctg ctttggtaaa acgaaccgga accagtgccg gagctgcatg gcttgcctgc
     2641 tgacgttccg gtgaaacatt acgatacagc ggtgtacgct gcattgcatt acgacccaga
     2701 cgcgcagcca gggcttccat tgctgccggt ggcatttctt ggccatgtgc tgcacccgct
     2761 gcacggctaa tgctttcatt catcagggtg ccacctaaat catttgcacc ggcttgcagg
     2821 cacagttgtg caccatgttc acccatttta acccagctcg tctgaatatt atcaatcaga
     2881 ccatgaaatg ccagacgacc aacggcatgc atcagaattg cttcacgcag cgtcggaccc
     2941 tgacgtgcac ctttcttttt aaacagcgga gcttcttcat gaacaaacgg cagcggaaca
     3001 aattcggtca gaccaccagt atcacgttgc agttctgcca ggcgcagaat atgacgtgcc
     3061 caatgtttgt agctttccac atgaccaaac ataatggtac aggtggtacg cagacccagt
     3121 tcatgtgcgc tacgaaccac ggtcagccat tcttgggtat tcagtttatc aggacaaatc
     3181 tggcgacgaa cttcatcatc caggatttcc gctgcggtgc caggcagggt gctcagacct
     3241 gcgcttttca gatcactcag aaaggcgcta atgctggtgc ccagtgtggt tgcaccatgc
     3301 agaacttcca gcggactaaa tgcatgaatg tgcatatccg gacattcggt ttttgctgct
     3361 tcacaaatat ccagataggt acgacctgta taatccggat gaataccacc ttgcagacaa
     3421 acttcggttg ctccgcgatc ccatgcttct ttaacacgac gacgaatttc attcagatcc
     3481 acaatatacg gtttttcacg cagatcctct tgaatcggac cacggctaaa tgcacaaaag
     3541 ttgcagtggt actgacaaac gttggtataa ttgatgttgc ggttcacaac ataggtaatg
     3601 gtatcacctt ttgtctgacg acgcagacga tctgcggtat gggtcacatg ggtaaaatca
     3661 tgaccacgtg cattaaacag gtgaaccagt tctgcttctt ccagacgctc accggcaaca
     3721 catttttcaa caatatcaat ggtctggcta ccaacacgat caacctggct tgcggtcggg
     3781 gtaactgcca gacatgcaat atcaccaatg gtggtgctac ctgctttcca aatatcgctg
     3841 gtcgccagac cgctacaatc gctatgacgc agaacatcgg catgcagacc cggatcaatc
     3901 caacgatcac gttcttcaat atatgccgga tacacggtaa tacgttcggt cagggtttta
     3961 ccaccacgat cggtatcttc actcagacga tccagatgag gccacggtgc ttcaggatta
     4021 acatgatccg gtgtaaccgg actaacacca ccccaatcat taatacctgc acgaatcaga
     4081 tcggtcagat cgccatcaaa caggttaggc ggaacctgaa tgctcattgc gctacccaga
     4141 accagacggg caactgctgt ggtccaacac agttcatcca tgctcggttc cggtgcatta
     4201 accattttcg tacccggttt tgcacgaaag ttctggataa tcacttcttg aacatgacca
     4261 taacgatcat gcagatcacg cagtgcaaac aggctttcca ggcgttcacg acgggtttca
     4321 ccaataccaa tcagaatacc gctggtaatc ggaatatgtg cttcgcctgc cagacgcagg
     4381 gtttccagac gtgctgccgg atgtttatcc ggactaccat aatgcggacc accacgttcg
     4441 ctcagacgtt cgcttgcggt ttccagcatc agaccaaagc tcggtgcatg cggacgcagc
     4501 atctgatatt ctgctgcgct cagaacaccc atgttaaaat gcggcagcag acctgtttct
     4561 gcacgaacac gctgtgcaac ttcggcaaca taatctgcgg tggttgcatg acccagttgt
     4621 tgcagtgcat cacgtgctgc tgcataacgt gcttccggac gatcacccag ggtaaacagt
     4681 gcttcacgac aaccaactgc tgcaccggca cgtgcaatat ccagtgcctg atcaacggtc
     4741 agaaatgcct gaccaataac acgcggtgct tttgcaaagg tgcaataatg acaaacatca
     4801 cgacacagat gtgtcagcgg gataaaaact ttgcggctat aggtaataac attaccccaa
     4861 ccaatatcac gccatgcgct tgccagacct gccagttcac gccaatcctg aactgcttcc
     4921 agttcatagg ccagtgcttt atcatccaga cgaggctggc taccttttgc gctacgttca
     4981 acttcttgaa tcatgtttgg acctccctag gttgacaaaa ttatttctag aggggaattg
     5041 ttatccgctc acaattcccc tatagttagt cgtattaatt taagcttcgc gggatcgaga
     5101 tctcgcagac tagttctgac atgccagtcg gcgtagagga tcgagatctc gatcccgcga
     5161 aattgatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5221 ttttgtttaa acttcgcggt attattatga actgcggtat caaaatgaaa gtgattattc
     5281 cggttagccc gatcaatagc ctgaaaaccc gtctgagcga atttctgagc ggtgaagaac
     5341 gtaaaaatct gctgctgaat atgctgaagg atatcattaa agcactggat ggtctggata
     5401 ttgttattgt tagccgtgat gaagagatcc tggattttgc caaaaatgaa ctgaaagccg
     5461 aaaccatcaa agagaaatac aaaggcctga acaacgcaat caaacaggcg tttgaagaaa
     5521 tcgaagataa agaggtgatt atcattccgg cagatattcc gctgatcaaa aaaaagcaca
     5581 tcgaggacat tctgaagctg agcaaaaact atgatctgat tattgcaccg agccgtggtg
     5641 gtggcaccaa tctgctgtat ctgaaaagca aagatctgat cgagatcaaa tatgagggct
     5701 tcagctttct gaaacatctg gaagaagcca aaaaacgcaa tctgcgctat tatatctatg
     5761 atagcttcct gattagcgtg gatatcaata caccggaaga tctgggtgaa atctttattc
     5821 atggcaacga tacctacacc aaaaactacc tgaaaagcct gggtattgat gtggaaccga
     5881 aacattcaag cgcaggtcgt ttcgttgtta aacgtcgtta ataggcacgc taaggcggtg
     5941 cacccatgat ttttgtgatt accgttctga gcggtggtac aggcaccccg aaactgctgc
     6001 agggtctgaa acgtgttgtt aataatgaag aactggccgt gattgtgaat accggtgaag
     6061 atacctggat tggtgatctg tatctgagtc cggatgttga taccgtgctg tataccctgg
     6121 cagatctgat taacgaagaa acctggtatg gtgtgaaaga ggataccttt tatacccacg
     6181 aacagctgaa aaatctgggc tttgatgaag ttctgcgcat tggtgataaa gatcgtgcac
     6241 tgaaaatgca caaaacctat tatctgaaac gcggtcataa actgagcgaa gttgttgata
     6301 tggaaaaagt tgccctgggc attaaagcaa aagttattcc gatgaccgat gatcgtgtgg
     6361 aaaccaaaat tctggcaaaa gttgatggta aagtggacct gctgaaattc catgattttt
     6421 gggttaaacg caaaggtgat gttgaagtgc tggatgtgat ttatgaaaac agcctgtatg
     6481 caaaaccgtg cgaaaaagca gttgaagcca ttaaaaacag cgatctggtt attattggtc
     6541 cgagcaatcc gattaccagc atcggtccga ttctgagcct gaatggtatt aaagaactgc
     6601 tgaaagacaa aaaagttgtt gtggttagcc cgattgttgg taatagcgca gttagcggtc
     6661 cggcaggtaa actgatgaaa gccaaaggtt atgatgttag cgtgaaaggc atctacgagt
     6721 tctataaaga tattgtggat gtgctggtga tcgacaacgt ggataaagaa attgcaaaag
     6781 aaattccgtg cgaagtgctg attaccaata ccatcatgaa aaccctggat gataaagttc
     6841 gtctggccaa aaacatcatt gaattttgtg gtagcctgta atagcaaagt aaggaggtca
     6901 agtcatgtct caccacgacg cggttccgcc gccgctggtt ccgctgccgg acccggaccg
     6961 tgttgcgggt tctcgtccgc tggacctgcc ggaaatcatc cgtggtcgtc gttctgttcg
     7021 tcgtctgcgt accgacccgg ttgcgccgga actggttacc gcggttctgg aagcggcggc
     7081 gtgggcgccg tctccgcacg gtacccagcc gtggcgtttc gttgttctga cccagccgga
     7141 acgtaaacgt caactggcgg acgcgatggc gaccgcgtgg cgtcaccacc tggcgatgga
     7201 cggtcaggac gaagaaacca tcgcgcgtcg tctggcgggt tctcagcgtc gtctgtgcga
     7261 agcgccggtt ctgatcctgg tttgcctgga cccgtctgac ctggaccgtt acccggaccc
     7321 ggaacgtcag gcggcggaac gtactatggc gatccagtct ctgggtgcgg cgatccagaa
     7381 cctgctgctg atggcgtacc gtctgggtct ggacaccggc tggatgtgcg cgccgctgtt
     7441 ctgcccggcg gttgttcgtg aaatcctgga cctgccgccg gttctggaac cgcaggcgct
     7501 ggttaccctg ggttacgcgg cggcggaccc gcagcgtcgt ccgcgtcgtc cgctgcacca
     7561 actgatcgtt cgttgggaat aataggattg tgatatagga ggtaagatga tcaaggaaaa
     7621 gcgcaaagtt gaagtgattg gtctggaact gccgattttt aaaggcggcg aacagattaa
     7681 tctgagtgaa ctgattgccc agtatccgat tgaagatggt gacattattg ttattgcaga
     7741 aaccctgatt agcaaactgg aaggtggtgt tattgatcgt gataaaatta ttccgagcaa
     7801 agaagccatt gaactggcca aaaagactgg taaagatccg aaagttgttc aggttattct
     7861 ggatgaagcc aaagaaattg tgaaagttgg caaaaatttc atcatcaccg aaaccaaaca
     7921 tggctttgtt tgcgccaata gcggtgtgga tgaaagcaat atctataaag gtattaagat
     7981 cctgccgaaa aatccggatg aaagtgccga aaaaattcgc aaagaaattg aaaaactgac
     8041 cggcaaacgc gttggcgtta ttattagtga tagcgtgggt cgcccgtttc gcaaaggcgc
     8101 agttggtatt gccattggcg ttagcggcat tctggcactg tgggatcgca aaggtgaaaa
     8161 agatctgttt ggtcgtgaac tgaaaaccac cgaagtggcc attgccgatg aactggccag
     8221 catggccaat gtggttatgg gcgaagccga tgaaggcatt ccggttgtta ttattcgtgg
     8281 cgcaaatgtg ccgtttggca atggcaaagg ccgtgatctg attcgtccga aagaagaaga
     8341 tgtgtttcgc aattaatagt agcataaccc cttggggcct ctaaacgggt cttgaggggt
     8401 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
     8461 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     8521 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8581 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     8641 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     8701 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     8761 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     8821 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     8881 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     8941 tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact
     9001 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     9061 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     9121 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     9181 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     9241 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     9301 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     9361 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     9421 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     9481 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     9541 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     9601 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     9661 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     9721 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag
     9781 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     9841 gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9901 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9961 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10021 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
    10081 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    10141 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    10201 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10261 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10321 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10381 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10441 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10501 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10561 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    10621 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    10681 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
    10741 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
    10801 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
    10861 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
    10921 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
    10981 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
    11041 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
    11101 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
    11161 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
    11221 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
    11281 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
    11341 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
    11401 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
    11461 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
    11521 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
    11581 cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa
    11641 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
    11701 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
    11761 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc
    11821 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
    11881 gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg
    11941 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    12001 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
    12061 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
    12121 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
    12181 aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca
    12241 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
    12301 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
    12361 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
    12421 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
    12481 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg
    12541 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
    12601 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
    12661 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
    12721 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
    12781 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
    12841 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
    12901 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
    12961 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
    13021 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
    13081 ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt
    13141 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca
    13201 tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg
    13261 aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg
    13321 atataggcg
//
