LOCUS       sequence-371204-       13324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High-yield production of coenzyme F420 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13324)
  AUTHORS   Last D, Hasan M, Rothenburger L, Braga D, Lackner G
  TITLE     High-yield production of coenzyme F420 in Escherichia coli by 
            fluorescence-based screening of multi-dimensional gene expression 
            space.
  JOURNAL   Metab Eng. 2022 Jul 18. pii: S1096-7176(22)00093-3. doi: 
            10.1016/j.ymben.2022.07.006.
  PUBMED    35863619
REFERENCE   2  (bases 1 to 13324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2022 Jul 18. pii: S1096-7176(22)00093-3. doi: 
            10.1016/j.ymben.2022.07.006."
COMMENT     Sequence Label: pDL52 (clone45)
FEATURES             Location/Qualifiers
     source          1..13324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    5041..5065
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    5190..5214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      8367..8413
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      8450..8905
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8537..8556)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8747..8768
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8997..9812)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8997..9812)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9720..9739
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9804..9823
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9934..10522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10423..10442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10676..10693
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10708..10847
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10773..10792)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10933..10955
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10949..11140)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(11949..13031)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     12993..13012
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(13032..13109)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     join(13315..13324,1..10)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccc
       61 tattatatca gcgaggtcag agatgccaac cgggaaattt ccagatgcgg cagtaaacgg
      121 ctatcccagg tttgcatcag atcgccattt tccggtctga tccaacaagc ctgcattccg
      181 ctgcgaattg ccccacccac gtcagtggtg agatcgtccc caacatgtaa gatctcgccg
      241 atcggcacgt tgagtttttc cgcagccaaa aagtacatat cgctgaacgg ttttgagcgc
      301 ccgtgcgggc cagcgcgcag cacaaactca aaataatccc ccaaaccaaa cagttccggc
      361 tgtgcattgc cgttagtgat cgccaccagc ggccatttct tcgccagctg ttttaaggtg
      421 tcgtgagttt gctgcgggac gtcgattcgg ctgcgccatt tggcaaagtt gatcattgct
      481 gcgtgtgcgc ctgcactggc ttcttcggca ctcagcccgg cgtcgagcat cgcttgttca
      541 atcgaacgaa aacgccagcg cgtcacgtcg tgataaatct cgggttccgc ttcccgtacc
      601 gcctggcgca ggcgttgcag atcttcattc tggaagctgc gcagcgccgg atgataattt
      661 tgcacaaagg taagcgcctc tcgctcggtg cgcaaaatca ccggacggtt atcgtaaagg
      721 gtatcatcca ggtcaaaggt gagcgccgag atgcgcccca aaggccggta aaaacgcatc
      781 ggtacgtctt tatccctatt atttgttcag caaatggccc attttctcgg ctttggtatc
      841 gagataatgt tcgttattgg ggttacgacc tacaatcaat ggtacgcgtt caacaatatt
      901 aatccctgct tcggtcagaa tttcgacttt tttcgggtta ttggttaaca agcggacttc
      961 attgacgcca aggagtttga acatatcagc gcaaagagtg aagtcgcgct catcagcggc
     1021 gaagcctaac tggtggttag cctctacggt atcgtaacct tgatcctgca gtgcgtaagc
     1081 gcggatttta ttcagcagac caatgttacg accttcctga cggtgataca gcaaaatacc
     1141 acggccttcc tcggcaattt gcgtcaatgc cgcttcgagc tggaagccac aatcgcagcg
     1201 caagctgaac agggcgtcac cggtcagaca ttcggaatgg acgcgcgcaa gtaccggggt
     1261 atgcccggaa atatcgccat agactagcgc gacatgatcg tgtccggttg ccagttcttc
     1321 aaatcccacc atcaggaaat cgccccatgg ggttggcagt ttggcttctg ccacacgttt
     1381 aagctgcatc aacgtacccc ctgaaaacac ctattatgca cctactaaat gcaggcaaac
     1441 atccgggcct acatgacgta tctctttgaa tttaaattgg ggggcgtcgg ctaatttctc
     1501 aagccctggc agcgagcata atccacgggc gtcgctgcct aatagtttag gtgcgatata
     1561 gacaatcagc tcatcgacta aacccgcctg cagcaatgcg ccagcgagcg ttggccccgc
     1621 ttccacccag atgctgttaa tttgctgttt acccagttgc atcatcagta caaccagatc
     1681 cagatgacct ttatgcgctg gaatcagcaa ggtacgcacc gtttccggcc actcacgaga
     1741 atcttcctgc gtacgcgcga accaggtttc gccgggctgc tgcacaatgc gatgtaccgg
     1801 cgtcacgcga ttttggctat caatcacaat acgtatcggc tgacggagat tttgttgcgg
     1861 atagagcgcc tgagtttgtt catccagttc agaccaacgc accgttaagg caggatcatc
     1921 cgccagcacc gtggcgctgc tggttaaaat ggcatgactt tgcgcgcgca gtagttgtac
     1981 atcacgccgc gcctggggcg aagtgatcca ctggctttcg ccgctcgcca tcgccgtgcg
     2041 accatcaagc gatgcgccaa gttttaactg aatataagga aagccggtgc gcatccgctt
     2101 gagaaagcct ttattcaatt gctcggcttc actcatcatc aggccgtggc tgacgtcaat
     2161 gccagcctgt tgcagacggt aaagtccacg cccagcgacc tgcgggttag gatcttgcat
     2221 cgaggcaacc acgcgcgcta cgccagcggc gatgagtgcg tcacagcacg gtggcgtacg
     2281 accatgatgg ctacagggtt cgagtgtgac ataggcggtc gcacctttgg ctttttcacc
     2341 cgccatacgc aacgcgtgta cttcggcatg tggttcaccc gcacgttggt ggtaaccttc
     2401 accgacaatt tcgccatctt tgacaatgac gcacccgaca ttcgggttgg gatgcgtggt
     2461 aaaacgtcct cgttgcgcca gctttagcgc ccgcgccatg taatactcgt cctgcatccc
     2521 tgaacgtcct ttgatgacct attactgttc ggcttcgctc agcacctgat gaccatgacc
     2581 gcgaaccagg cgacctgctt tggtaaaacg aaccggaacc agtgccggag ctgcatggct
     2641 tgcctgctga cgttccggtg aaacattacg atacagcggt gtacgctgca ttgcattacg
     2701 acccagacgc gcagccaggg cttccattgc tgccggtggc atttcttggc catgtgctgc
     2761 acccgctgca cggctaatgc tttcattcat cagggtgcca cctaaatcat ttgcaccggc
     2821 ttgcaggcac agttgtgcac catgttcacc cattttaacc cagctcgtct gaatattatc
     2881 aatcagacca tgaaatgcca gacgaccaac ggcatgcatc agaattgctt cacgcagcgt
     2941 cggaccctga cgtgcacctt tctttttaaa cagcggagct tcttcatgaa caaacggcag
     3001 cggaacaaat tcggtcagac caccagtatc acgttgcagt tctgccaggc gcagaatatg
     3061 acgtgcccaa tgtttgtagc tttccacatg accaaacata atggtacagg tggtacgcag
     3121 acccagttca tgtgcgctac gaaccacggt cagccattct tgggtattca gtttatcagg
     3181 acaaatctgg cgacgaactt catcatccag gatttccgct gcggtgccag gcagggtgct
     3241 cagacctgcg cttttcagat cactcagaaa ggcgctaatg ctggtgccca gtgtggttgc
     3301 accatgcaga acttccagcg gactaaatgc atgaatgtgc atatccggac attcggtttt
     3361 tgctgcttca caaatatcca gataggtacg acctgtataa tccggatgaa taccaccttg
     3421 cagacaaact tcggttgctc cgcgatccca tgcttcttta acacgacgac gaatttcatt
     3481 cagatccaca atatacggtt tttcacgcag atcctcttga atcggaccac ggctaaatgc
     3541 acaaaagttg cagtggtact gacaaacgtt ggtataattg atgttgcggt tcacaacata
     3601 ggtaatggta tcaccttttg tctgacgacg cagacgatct gcggtatggg tcacatgggt
     3661 aaaatcatga ccacgtgcat taaacaggtg aaccagttct gcttcttcca gacgctcacc
     3721 ggcaacacat ttttcaacaa tatcaatggt ctggctacca acacgatcaa cctggcttgc
     3781 ggtcggggta actgccagac atgcaatatc accaatggtg gtgctacctg ctttccaaat
     3841 atcgctggtc gccagaccgc tacaatcgct atgacgcaga acatcggcat gcagacccgg
     3901 atcaatccaa cgatcacgtt cttcaatata tgccggatac acggtaatac gttcggtcag
     3961 ggttttacca ccacgatcgg tatcttcact cagacgatcc agatgaggcc acggtgcttc
     4021 aggattaaca tgatccggtg taaccggact aacaccaccc caatcattaa tacctgcacg
     4081 aatcagatcg gtcagatcgc catcaaacag gttaggcgga acctgaatgc tcattgcgct
     4141 acccagaacc agacgggcaa ctgctgtggt ccaacacagt tcatccatgc tcggttccgg
     4201 tgcattaacc attttcgtac ccggttttgc acgaaagttc tggataatca cttcttgaac
     4261 atgaccataa cgatcatgca gatcacgcag tgcaaacagg ctttccaggc gttcacgacg
     4321 ggtttcacca ataccaatca gaataccgct ggtaatcgga atatgtgctt cgcctgccag
     4381 acgcagggtt tccagacgtg ctgccggatg tttatccgga ctaccataat gcggaccacc
     4441 acgttcgctc agacgttcgc ttgcggtttc cagcatcaga ccaaagctcg gtgcatgcgg
     4501 acgcagcatc tgatattctg ctgcgctcag aacacccatg ttaaaatgcg gcagcagacc
     4561 tgtttctgca cgaacacgct gtgcaacttc ggcaacataa tctgcggtgg ttgcatgacc
     4621 cagttgttgc agtgcatcac gtgctgctgc ataacgtgct tccggacgat cacccagggt
     4681 aaacagtgct tcacgacaac caactgctgc accggcacgt gcaatatcca gtgcctgatc
     4741 aacggtcaga aatgcctgac caataacacg cggtgctttt gcaaaggtgc aataatgaca
     4801 aacatcacga cacagatgtg tcagcgggat aaaaactttg cggctatagg taataacatt
     4861 accccaacca atatcacgcc atgcgcttgc cagacctgcc agttcacgcc aatcctgaac
     4921 tgcttccagt tcataggcca gtgctttatc atccagacga ggctggctac cttttgcgct
     4981 acgttcaact tcttgaatca tgtttggacc tacctaggtt gacaaaatta tttctagagg
     5041 ggaattgtta tccgctcaca attcccctat agtgagtcgt atgaatttaa gcttcgcggg
     5101 atcgagatct cgcagactag ttctgacatg ccagtcggcg tagaggatcg agatctcgat
     5161 cccgcgaaat ttatacgact cactataggg gaattgtgag cggataacaa ttcccctcta
     5221 gaaataattt tgtttaaact tcgcggtatt attatgaact gcggtatcaa aatgaaagtg
     5281 attattccgg ttagcccgat caatagcctg aaaacccgtc tgagcgaatt tctgagcggt
     5341 gaagaacgta aaaatctgct gctgaatatg ctgaaggata tcattaaagc actggatggt
     5401 ctggatattg ttattgttag ccgtgatgaa gagatcctgg attttgccaa aaatgaactg
     5461 aaagccgaaa ccatcaaaga gaaatacaaa ggcctgaaca acgcaatcaa acaggcgttt
     5521 gaagaaatcg aagataaaga ggtgattatc attccggcag atattccgct gatcaaaaaa
     5581 aagcacatcg aggacattct gaagctgagc aaaaactatg atctgattat tgcaccgagc
     5641 cgtggtggtg gcaccaatct gctgtatctg aaaagcaaag atctgatcga gatcaaatat
     5701 gagggcttca gctttctgaa acatctggaa gaagccaaaa aacgcaatct gcgctattat
     5761 atctatgata gcttcctgat tagcgtggat atcaatacac cggaagatct gggtgaaatc
     5821 tttattcatg gcaacgatac ctacaccaaa aactacctga aaagcctggg tattgatgtg
     5881 gaaccgaaac attcaagcgc aggtcgtttc gttgttaaac gtcgttaata ggaacgctac
     5941 ggaggtgcac ccatgatttt tgtgattacc gttctgagcg gtggtacagg caccccgaaa
     6001 ctgctgcagg gtctgaaacg tgttgttaat aatgaagaac tggccgtgat tgtgaatacc
     6061 ggtgaagata cctggattgg tgatctgtat ctgagtccgg atgttgatac cgtgctgtat
     6121 accctggcag atctgattaa cgaagaaacc tggtatggtg tgaaagagga taccttttat
     6181 acccacgaac agctgaaaaa tctgggcttt gatgaagttc tgcgcattgg tgataaagat
     6241 cgtgcactga aaatgcacaa aacctattat ctgaaacgcg gtcataaact gagcgaagtt
     6301 gttgatatgg aaaaagttgc cctgggcatt aaagcaaaag ttattccgat gaccgatgat
     6361 cgtgtggaaa ccaaaattct ggcaaaagtt gatggtaaag tggacctgct gaaattccat
     6421 gatttttggg ttaaacgcaa aggtgatgtt gaagtgctgg atgtgattta tgaaaacagc
     6481 ctgtatgcaa aaccgtgcga aaaagcagtt gaagccatta aaaacagcga tctggttatt
     6541 attggtccga gcaatccgat taccagcatc ggtccgattc tgagcctgaa tggtattaaa
     6601 gaactgctga aagacaaaaa agttgttgtg gttagcccga ttgttggtaa tagcgcagtt
     6661 agcggtccgg caggtaaact gatgaaagcc aaaggttatg atgttagcgt gaaaggcatc
     6721 tacgagttct ataaagatat tgtggatgtg ctggtgatcg acaacgtgga taaagaaatt
     6781 gcaaaagaaa ttccgtgcga agtgctgatt accaatacca tcatgaaaac cctggatgat
     6841 aaagttcgtc tggccaaaaa catcattgaa ttttgtggta gcctgtaata gcaaagtaag
     6901 gagggcaagt catgtctcac cacgacgcgg ttccgccgcc gctggttccg ctgccggacc
     6961 cggaccgtgt tgcgggttct cgtccgctgg acctgccgga aatcatccgt ggtcgtcgtt
     7021 ctgttcgtcg tctgcgtacc gacccggttg cgccggaact ggttaccgcg gttctggaag
     7081 cggcggcgtg ggcgccgtct ccgcacggta cccagccgtg gcgtttcgtt gttctgaccc
     7141 agccggaacg taaacgtcaa ctggcggacg cgatggcgac cgcgtggcgt caccacctgg
     7201 cgatggacgg tcaggacgaa gaaaccatcg cgcgtcgtct ggcgggttct cagcgtcgtc
     7261 tgtgcgaagc gccggttctg atcctggttt gcctggaccc gtctgacctg gaccgttacc
     7321 cggacccgga acgtcaggcg gcggaacgta ctatggcgat ccagtctctg ggtgcggcga
     7381 tccagaacct gctgctgatg gcgtaccgtc tgggtctgga caccggctgg atgtgcgcgc
     7441 cgctgttctg cccggcggtt gttcgtgaaa tcctggacct gccgccggtt ctggaaccgc
     7501 aggcgctggt taccctgggt tacgcggcgg cggacccgca gcgtcgtccg cgtcgtccgc
     7561 tgcaccaact gatcgttcgt tgggaataat aggattgtga tataggaggt aagatgatca
     7621 aggaaaagcg caaagttgaa gtgattggtc tggaactgcc gatttttaaa ggcggcgaac
     7681 agattaatct gagtgaactg attgcccagt atccgattga agatggtgac attattgtta
     7741 ttgcagaaac cctgattagc aaactggaag gtggtgttat tgatcgtgat aaaattattc
     7801 cgagcaaaga agccattgaa ctggccaaaa agactggtaa agatccgaaa gttgttcagg
     7861 ttattctgga tgaagccaaa gaaattgtga aagttggcaa aaatttcatc atcaccgaaa
     7921 ccaaacatgg ctttgtttgc gccaatagcg gtgtggatga aagcaatatc tataaaggta
     7981 ttaagatcct gccgaaaaat ccggatgaaa gtgccgaaaa aattcgcaaa gaaattgaaa
     8041 aactgaccgg caaacgcgtt ggcgttatta ttagtgatag cgtgggtcgc ccgtttcgca
     8101 aaggcgcagt tggtattgcc attggcgtta gcggcattct ggcactgtgg gatcgcaaag
     8161 gtgaaaaaga tctgtttggt cgtgaactga aaaccaccga agtggccatt gccgatgaac
     8221 tggccagcat ggccaatgtg gttatgggcg aagccgatga aggcattccg gttgttatta
     8281 ttcgtggcgc aaatgtgccg tttggcaatg gcaaaggccg tgatctgatt cgtccgaaag
     8341 aagaagatgt gtttcgcaat taatagtagc ataacccctt ggggcctcta aacgggtctt
     8401 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
     8461 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8521 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8581 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8641 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8701 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8761 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8821 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     8881 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8941 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag
     9001 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     9061 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     9121 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     9181 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     9241 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
     9301 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     9361 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     9421 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     9481 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     9541 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     9601 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     9661 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     9721 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta
     9781 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     9841 gcagacagtt ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     9901 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     9961 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    10021 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    10081 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    10141 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    10201 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    10261 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    10321 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    10381 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    10441 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    10501 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    10561 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    10621 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    10681 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
    10741 cggtatttca caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
    10801 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
    10861 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
    10921 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
    10981 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct
    11041 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
    11101 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
    11161 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
    11221 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
    11281 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag
    11341 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg
    11401 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc
    11461 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta
    11521 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg
    11581 acaggagcac gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct
    11641 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc
    11701 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga
    11761 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca
    11821 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
    11881 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg
    11941 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    12001 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
    12061 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
    12121 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
    12181 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca
    12241 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
    12301 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
    12361 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
    12421 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
    12481 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
    12541 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
    12601 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
    12661 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
    12721 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
    12781 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
    12841 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
    12901 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
    12961 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
    13021 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
    13081 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc
    13141 ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa
    13201 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
    13261 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
    13321 gtcg
//
