LOCUS       sequence-371212-        9462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Third generation lentiviral vector expressing Stim1-wt fused to 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Zamponi E, Meehl JB, Voeltz GK
  TITLE     The ER ladder is a unique morphological feature of developing 
            mammalian axons.
  JOURNAL   Dev Cell. 2022 Jun 6;57(11):1369-1382.e6. doi: 
            10.1016/j.devcel.2022.05.002. Epub 2022 May 23.
  PUBMED    35609616
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2022.05.002"; journalName: "Dev Cell"; date: 
            "2022-06-6- 6"; volume: "57"; issue: "11"; pages: "1369-1382.e6"
COMMENT     Sequence Label: pLV-mCherry-Stim1
FEATURES             Location/Qualifiers
     source          1..9462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(115..134)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      928..1516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1417..1436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1670..1687
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1804..1825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1840..1845,1846..1863,1864..1870)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1878..1894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1883..1905
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1902..1918
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1902..1918
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1937..1957
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1939..1957
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1985..2211
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2212..2392
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2439..2564
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3057..3290
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3475..3519
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3786..3902
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3925..4228
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4229..4432
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4383..4403
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4429..4453
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4515..4524
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4590..5294
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4732..4750)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5000..5019
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7290..7878
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7343..7363)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7761..7772)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7965..8198
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8270..8391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8307..8326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8361..8380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8431..8566
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8586..8605)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8587..8605)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8615..8632)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8615..8631)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8624..8646)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8773..9228
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8860..8879)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9070..9091
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9254..9358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9359..9427,9428..9462,1..757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
       61 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      121 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      181 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      301 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      361 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      421 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      481 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      541 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      601 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      661 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      721 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
      781 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      841 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      901 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      961 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1021 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1081 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1141 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1201 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1261 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1321 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1381 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1441 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1501 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1561 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1621 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1681 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     1741 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     1801 aattaatgtg agttagctca ctcattaggc accccaggct ttccacttta tgcttccggc
     1861 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     1921 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     1981 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     2041 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     2101 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     2161 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     2221 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     2281 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     2341 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     2401 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     2461 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     2521 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     2581 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     2641 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     2701 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     2761 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     2821 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     2881 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     2941 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     3001 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     3061 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     3121 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     3181 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     3241 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     3301 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     3361 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     3421 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     3481 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     3541 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     3601 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     3661 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     3721 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     3781 ttaactttta aaagaaaagg ggggattggg gggtatagtg caggggaaag aatagtagac
     3841 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt
     3901 ttatcgataa gcttgggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     3961 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     4021 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     4081 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     4141 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     4201 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     4261 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     4321 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     4381 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     4441 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
     4501 actctagagg atccgccacc atggatgtat gcgtccgtct tgccctgtgg ctcctctggg
     4561 gactcctcct gcaccagggc cagagcctcg tgagcaaggg cgaggaggat aacatggcca
     4621 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     4681 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     4741 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     4801 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     4861 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     4921 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     4981 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     5041 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     5101 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     5161 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     5221 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     5281 acgagctgta caagagccat agtcacagtg agaaggcgac aggaaccagc tcgggggcca
     5341 actctgagga gtccactgca gcagagtttt gccgaattga caagcccctg tgtcacagtg
     5401 aggatgagaa actcagcttc gaggcagtcc gtaacatcca caaactgatg gacgatgatg
     5461 ccaatggtga tgtggatgtg gaagaaagtg atgagttcct gagggaagac ctcaattacc
     5521 atgacccaac agtgaaacac agcaccttcc atggtgagga taagctcatc agcgtggagg
     5581 acctgtggaa ggcatggaag tcatcagaag tatacaattg gaccgtggat gaggtggtac
     5641 agtggctgat cacatatgtg gagctgcctc agtatgagga gaccttccgg aagctgcagc
     5701 tcagtggcca tgccatgcca aggctggctg tcaccaacac caccatgaca gggactgtgc
     5761 tgaagatgac agaccggagt catcggcaga agctgcagct gaaggctctg gatacagtgc
     5821 tctttgggcc tcctctcttg actcgccata atcacctcaa ggacttcatg ctggtggtgt
     5881 ctatcgttat tggtgtgggc ggctgctggt ttgcctatat ccagaaccgt tactccaagg
     5941 agcacatgaa gaagatgatg aaggacttgg aggggttaca ccgagctgag cagagtctgc
     6001 atgaccttca ggaaaggctg cacaaggccc aggaggagca ccgcacagtg gaggtggaga
     6061 aggtccatct ggaaaagaag ctgcgcgatg agatcaacct tgctaagcag gaagcccagc
     6121 ggctgaagga gctgcgggag ggtactgaga atgagcggag ccgccaaaaa tatgctgagg
     6181 aggagttgga gcaggttcgg gaggccttga ggaaagcaga gaaggagcta gaatctcaca
     6241 gctcatggta tgctccagag gcccttcaga agtggctgca gctgacacat gaggtggagg
     6301 tgcaatatta caacatcaag aagcaaaatg ctgagaagca gctgctggtg gccaaggagg
     6361 gggctgagaa gataaaaaag aagagaaaca cactctttgg caccttccac gtggcccaca
     6421 gctcttccct ggatgatgta gatcataaaa ttctaacagc taagcaagca ctgagcgagg
     6481 cgacagcagc attgcgggag cgcctgcacc gctggcaaca gatcgagatc ctctgtggct
     6541 tccagattgt caacaaccct ggcatccact cactggtggc tgccctcaac atagacccca
     6601 gctggatggg cagtacacgc cccaaccctg ctcacttcat catgactgac gacgtggatg
     6661 acatggatga ggagattgtg tctcccttgt ccatgcagtc ccctagcctg cagagcagtg
     6721 ttcggcagcg cctgacggag ccacagcatg gcctgggatc tcagagggat ttgacccatt
     6781 ccgattcgga gtcctccctc cacatgagtg accgccagcg tgtggccccc aaacctcctc
     6841 agatgagccg tgctgcagac gaggctctca atgccatgac ttccaatggc agccaccggc
     6901 tgatcgaggg ggtccaccca gggtctctgg tggagaaact gcctgacagc cctgccctgg
     6961 ccaagaaggc attactggcg ctgaaccatg ggctggacaa ggcccacagc ctgatggagc
     7021 tgagcccctc agccccacct ggtggctctc cacatttgga ttcttcccgt tctcacagcc
     7081 ccagctcccc agacccagac acaccatctc cagttgggga cagccgagcc ctgcaagcca
     7141 gccgaaacac acgcattccc cacctggctg gcaagaaggc tgtggctgag gaggataatg
     7201 gctctattgg cgaggaaaca gactccagcc caggccggaa gaagtttccc ctcaaaatct
     7261 ttaagaagcc tcttaagaag taggtcgaca atcaacctct ggattacaaa atttgtgaaa
     7321 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7381 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7441 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7501 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7561 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7621 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7681 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7741 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7801 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7861 tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa
     7921 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7981 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8041 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8101 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8161 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8221 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     8281 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8341 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8401 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8461 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8521 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     8581 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     8641 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     8701 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     8761 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     8821 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     8881 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     8941 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     9001 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     9061 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     9121 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     9181 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     9241 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     9301 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     9361 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     9421 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct ga
//
