LOCUS       sequence-371223-        9377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP48
FEATURES             Location/Qualifiers
     source          1..9377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             173..689
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    753..1094
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1161..1577
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1507..1529
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1545..1561
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1590..1599
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1599..1622
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1635..1661
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1677..1701
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4651..4675)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4688..5255
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4705..5257
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4849..4866)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5076..5095
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5269..5868
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5269..5288)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5765..5785
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5944..6458
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6620..6636
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6644..6650,6651..6668,6669..6674))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6689..6710
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6827..6844)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6998..7586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7078..7097)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7757..8548,8549..8617))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8380..8399
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8618..8722)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8790..8808
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8846..8868)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8968..8987
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9181..9203
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9331..9350
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tgatgccggc cacgatgcgt ccggcgtaga ggcgattagt ccaatttgtt aaagacagga
       61 tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt
      121 acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
      181 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
      241 tacataactg agaatagaga agttcagatc aaggttagga acagagagac agcagaatat
      301 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      361 ggtccccaga tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg
      421 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
      481 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
      541 cggcgcgcca gtcctccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct
      601 cttgctgttt gcatccgaat cgtggactcg ctgatccttg ggagggtctc ctcagattga
      661 ttgactgccc acctcggggg tctttcattt ggaggttcca ccgagatttg gagacccctg
      721 cccagggacc accgaccccc ccgccgggag gtaagctggc cagcggtcgt ttcgtgtctg
      781 tctctgtctt tgtgcgtgtt tgtgccggca tctaatgttt gcgcctgcgt ctgtactagt
      841 tagctaacta gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg
      901 gccgcaaccc tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag
      961 gaagggagtc gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga
     1021 acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg
     1081 cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc
     1141 tgtatttgtc tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc
     1201 actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt
     1261 gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca
     1321 cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg
     1381 gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc
     1441 cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt
     1501 ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca
     1561 ctccttctct aggcgccgga attagatctg ccaccatgga ctacaaggat gacgatgaca
     1621 agctcgatgg aggataccca tacgatgttc cagattacgc tgctcgaggt tatcaaacaa
     1681 gtttgtacaa aaaagttggc atggccccgc ggctgcagct ggagaaggcg gcctggcgct
     1741 gggcggagac ggtgcggccc gaggaggtgt cgcaggagca catcgagacc gcttaccgca
     1801 tctggctgga gccctgcatt cgcggcgtgt gcagacgaaa ctgcaaagga aatccgaatt
     1861 gcttggttgg tattggtgag catatttggt taggagaaat agatgaaaat agttttcata
     1921 acatcgatga tcccaactgt gagaggagaa aaaagaactc atttgtgggc ctgactaacc
     1981 ttggagccac ttgttatgtc aacacatttc ttcaagtgtg gtttctcaac ttggagcttc
     2041 ggcaggcact ctacttatgt ccaagcactt gtagtgacta catgctggga gacggcatcc
     2101 aagaagaaaa agattatgag cctcaaacaa tttgtgagca tctccagtac ttgtttgcct
     2161 tgttgcaaaa cagtaatagg cgatacattg atccatcagg atttgttaaa gccttgggcc
     2221 tggacactgg acaacagcag gatgctcaag aattttcaaa gctctttatg tctctattgg
     2281 aagatacttt gtctaaacaa aagaatccag atgtgcgcaa tattgttcaa cagcagttct
     2341 gtggagaata tgcctatgta actgtttgca accagtgtgg cagagagtct aagcttttgt
     2401 caaaatttta tgagctggag ttaaatatcc aaggccacaa acagttaaca gattgtatct
     2461 cggaattttt gaaggaagaa aaattagaag gagacaatcg ctatttttgc gagaactgtc
     2521 aaagcaaaca gaatgcaaca agaaagattc gacttcttag ccttccttgc actctgaact
     2581 tgcagctaat gcgttttgtc tttgacaggc aaactggaca taagaaaaag ctgaatacct
     2641 acattggctt ctcagaaatt ttggatatgg agccttatgt ggaacataaa ggtgggtcct
     2701 acgtgtatga actcagcgca gtcctcatac acagaggagt gagtgcttat tctggccact
     2761 acatcgccca cgtgaaagat ccacagtctg gtgaatggta taagtttaat gatgaagaca
     2821 tagaaaagat ggaggggaag aaattacaac tagggattga ggaagatcta gcagaacctt
     2881 ctaagtctca gacacgtaaa cccaagtgtg gcaaaggaac tcattgctct cgaaatgcat
     2941 atatgttggt ttatagactg caaactcaag aaaagcccaa cactactgtt caagttccag
     3001 cctttcttca agagctggta gatcgggata attccaaatt tgaggagtgg tgtattgaaa
     3061 tggctgagat gcgtaagcaa agtgtggata aaggaaaagc aaaacacgaa gaggttaagg
     3121 agctgtacca aaggttacct gctggagctg agccctatga gtttgtctct ctggaatggc
     3181 tgcaaaagtg gttggatgaa tcaacaccta ccaaacctat tgataatcac gcttgcctgt
     3241 gttcccatga caagcttcac ccggataaaa tatcaattat gaagaggata tctgaatatg
     3301 cagctgacat tttctatagt agatatggag gaggtccaag actaactgtg aaagccctgt
     3361 gtaaggaatg tgtagtagaa cgttgtcgca tattgcgtct gaagaaccaa ctaaatgaag
     3421 attataaaac tgttaataat ctgctgaaag cagcagtaaa gggcagcgat ggattttggg
     3481 tggggaagtc ctccttgcgg agttggcgcc agctagctct tgaacagctg gatgagcaag
     3541 atggtgatgc agaacaaagc aacggaaaga tgaacggtag caccttaaat aaagatgaat
     3601 caaaggaaga aagaaaagaa gaggaggaat taaattttaa tgaagatatt ctgtgtccac
     3661 atggtgagtt atgcatatct gaaaatgaaa gaaggcttgt ttctaaagag gcttggagca
     3721 aactgcagca gtactttcca aaggctcctg agtttccaag ttacaaagag tgctgttcac
     3781 agtgcaagat tttagaaaga gaaggggaag aaaatgaagc cttacataag atgattgcaa
     3841 acgagcaaaa gacttctctc ccaaatttgt tccaggataa aaacagaccg tgtctcagta
     3901 actggccaga ggatacggat gtcctctaca tcgtgtctca gttctttgta gaagagtggc
     3961 ggaaatttgt tagaaagcct acaagatgca gccctgtgtc atcagttggg aacagtgctc
     4021 ttttgtgtcc ccacgggggc ctcatgttta catttgcttc catgaccaaa gaagattcta
     4081 aacttatagc tctcatatgg cccagtgagt ggcaaatgat acaaaagctc tttgttgtgg
     4141 atcatgtaat taaaatcacg agaattgaag tgggagatgt aaacccttca gaaacacagt
     4201 atatttctga gcccaaactc tgtccagaat gcagagaagg cttattgtgt cagcagcaga
     4261 gggacctgcg tgaatacact caagccacca tctatgtcca taaagttgtg gataataaaa
     4321 aggtgatgaa ggattcggct ccggaactga atgtgagtag ttctgaaaca gaggaggaca
     4381 aggaagaagc taaaccagat ggagaaaaag atccagattt taatcaaatc atgcatgcat
     4441 tttcagttgc tccttttgac cagaatttgt caattgatgg aaagatttta agtgatgact
     4501 gtgccaccct aggcaccctt ggcgtcattc ctgaatctgt cattttattg aaggctgatg
     4561 aaccaattgc agattatgct gcaatggatg atgtcatgca agtttgtatg ccagaagaag
     4621 ggtttaaagg tactggtctt cttggacatt aaccaacttt cttgtacaaa gtggttcgat
     4681 aacgaattcc gccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     4741 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     4801 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     4861 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     4921 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     4981 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     5041 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     5101 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     5161 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     5221 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat gaccgagtac
     5281 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     5341 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     5401 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     5461 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     5521 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     5581 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     5641 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     5701 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     5761 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     5821 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatg catcgatgtc
     5881 gacctgcagc caagcttatc gataaaataa aagattttat ttagtctcca gaaaaagggg
     5941 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
     6001 gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga acagagagac
     6061 agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     6121 aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga accatcagat
     6181 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     6241 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     6301 acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac ccgtgtatcc
     6361 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     6421 tctgagtgat tgactacccg tcagcggggg tctttcatgg gtaacagttt cttgaagttg
     6481 gagaacaaca ttctgagggt aggagtcgaa tattaagtaa tcctgactca attagccact
     6541 gttttgaatc cacatactcc aatactcctg aaatagttca ttatggacag cgcagaaaga
     6601 gctggggaga attgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6661 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6721 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6781 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6841 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6901 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6961 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7021 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7081 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7141 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7201 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7261 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7321 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7381 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7441 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7501 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7561 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7621 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7681 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7741 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7801 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7861 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7921 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7981 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8041 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8101 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8161 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8221 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8281 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8341 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8401 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8461 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8521 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8581 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8641 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8701 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     8761 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     8821 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     8881 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     8941 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     9001 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     9061 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     9121 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     9181 cccagtcacg acgttgtaaa acgacggcgc aaggaatggt gcatgcaagg agatggcgcc
     9241 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     9301 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     9361 cgcacctgtg gcgccgg
//
