LOCUS       sequence-371228-        6849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Codon optimized blue fluorescent protein Electra2 in C.elegans 
            expresison plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, 
            Jia JM, Koster RW, Namikawa K, Piatkevich KD
  TITLE     Dual-expression system for blue fluorescent protein optimization.
  JOURNAL   Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0.
  PUBMED    35715437
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-06-17"; volume: "12(1):10190. doi"; pages: " 
            10.1038/s41598-022-13214-0"
COMMENT     Sequence Label: pSF11-wElectra2
FEATURES             Location/Qualifiers
     source          1..6849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4419..4441)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4541..4560
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4642..4660)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4728..4832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4833..4901,4902..5693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5051..5070)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5864..6452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6353..6372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6606..6623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6740..6761
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6776..6781,6782..6799,6800..6806)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6814..6830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6819..6841
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(6838..6849,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(6838..6849,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
       61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
      121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
      181 aaaaaaaaaa gtgagggggc tccctgcccc cccccccctc ccccccccct cttctccttg
      241 aagctcatcc agacgtcgct gtcctcctcc tcctcgtccc cggggcgctg tgtgtgtgtg
      301 tgtgtgctcc gtgagcatgt agcagcttca gcccatttaa aggcgcacac tctctgcatc
      361 tctgcgtgtc tcgacactcg ttttttctct tcttttctgc tgcacacaca cacacacaca
      421 cacacacgct catgccagag cacttcttgc tccggggggc tccgcgcgga gcccgagagg
      481 acctcctcct cctttttctt cttcgctttt tagttcttct tgcctcggtc tcgccccttg
      541 ttttgctctc catgtagtat atacatctgg gtgtgtgtgt gtgtgtgcac ctttggagca
      601 ttttttgaac ctttccagaa ctttggagca acattttcag cttgttcagt gcacttttta
      661 ggctgaaaat cctattttta ggttttcgaa agtttttttg gagcattttt cgtgcaattt
      721 aggtgcaaaa ttaaaaaaaa gggttttgta gtgaattaaa atttttttga aactttcgcc
      781 tttttaaaaa aatgtttcta atatctttaa aaattggaat attttggaaa acaatttgtt
      841 ttcaattggc aatttattaa ttctgagctt ctgagcgtaa attttttttt attttggagc
      901 aattctgaga tgtcttagtg catttttcgg gcatacataa aaaaaaattt atcttaataa
      961 taaaaacctt aaatttaggc ttcaccgtct ggaaattcaa ttttttcagc tgaaaaactt
     1021 tcaaaaaaaa aaaaattaaa ttttcacatt tattttctct ggaattttgg agcaatttca
     1081 aagctgtctt agtgcatttt tagtacacaa atattttttc atttttctta aattttaata
     1141 atctaagctt aaataaaggc ttcaccgtct gaaaatgcaa cttttttcag atgaaatatt
     1201 aaaaaaaaaa cctcttttat taattttgtg ctccaaactt tcccacggta cgcaaatcat
     1261 gtaatatgta cttttaaaca ttatttttcc aatcaaaaat atccaacagc ttttccttac
     1321 aatttgaaaa tttttattaa aatttcaaaa aaattttttt tttttttaat ttcgaatttt
     1381 tttaaacttg ttgtaacttt tttccctgtc ggcgccaatt aacaaaatac gtagaatttt
     1441 gcgcttattg ttttgttagt taacaaattt ttgacaactt tcttatcagc tgagatttag
     1501 gtgtttcagt tcgcaatctg cgtctctcct tgctctcctc tattttaaaa cccccataac
     1561 tttgttcttg tgagagttat caaaaaattg tcaactaaca aaatatagag cacaaaaata
     1621 tgcacatttt gtatgttgac aattttttga tacctttttt agttttcgag atatcttagt
     1681 tttagtttag ctgtaccttt ttctggcaaa aaacttccaa atttcggcaa tttttcggca
     1741 aatttcccgg caaatttcgc caagtacatc gggctctcag acaagtggtc tgtgagtgga
     1801 agtcgataaa agtagtttac gtgctctccg tccccccgct tcttctccgt ctctgcttct
     1861 atttctccat tgactagtcc cactactttt tacggggcgg agggctctct tgcactccag
     1921 cttcttctct ttttgtgctc ttttttgccc gtatgtgggt gctcatttat ttttctttgg
     1981 ttttaaaata aattcggttt attaggctac tcgtaaaaat gaagaaaaaa cttcaaattc
     2041 aaacgacatt aaaaaaaaat ttaataaaaa ttttttgaat tgctcaaaag ccccacctat
     2101 atatatatat agctccgcct acttctcgca cagaccccgc ccactttttg actccgcccc
     2161 cttattggca aaagttcttg aaaaaaataa aaataaaaat tcaaattgaa acaatttttt
     2221 tttaatgaag tagttttttt ctaaattgat gaaaatttct aaacagttta caagcaccgc
     2281 ccactttttg actccgcccc tttgttggaa aaattcttgt aaaaaaaaat aaaaaataaa
     2341 agcttctctt tcaaacgatt tttagttggt ctttgtatga aaagtttttt tcctaaaata
     2401 gaaatgtctg aataatttgc aagctccgcc cttttcttac ccagactccg cccctttatt
     2461 gactaatttt tttttgtcca tttacctgaa gttcaaaaaa aaaatttaca gtttcataga
     2521 gtaataattt ttcaaaaatc tctttttcca atgtttaatg tgtcattata aacccgccca
     2581 gttttaagct ccgcccattt aggccccgcc catttatgac ccaaatattt ttttctctat
     2641 tcacccgtgg atttccctcc aacaagttac cccaaaaata aaaataaaaa ttcctgcagg
     2701 cgcccaccgg gatccggcgc gcctctagag gatccccggg attggccaaa ggacccaaag
     2761 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttggctagcg
     2821 tcgacggtac caaaaatggt ttccaaggga gaggaactta tcgaagagaa catgcgcatg
     2881 aaggttgtta tggagggatc tgttaacgga catcagttca agtgcactgg agagggagag
     2941 ggacgcccat acgagggagt tcaaactatg cgcatcaagg ttatcgaagg aggaccactt
     3001 ccattcgctt tcgacatcct cgctacctct ttccttttcg gatctaagac tttcattaag
     3061 tacccagctg atatcccaga cttcttcgag caatctttcc cagaaggatt cacctgggag
     3121 cgcgtcaccc gctacgagga cggaggagtt gttaccgtta cccaagatac ctccctcgag
     3181 gacggaggac ttgtttacaa cgttaaggta agtttaaaca tatatatact aactaaccct
     3241 gattatttaa attttcaggt tcgtggagtt aacttccatt ctaagggacc agttatgcaa
     3301 aagaagactg aaggatggga accattcact gaaatgatgt acccagctga cggaggactt
     3361 cgcggataca ccgacatcgc tcttaaggtc gacggaggag gacaccttca cgctaacatc
     3421 gttaccacct accgctctaa gaagactgtt ggaaatatta agatgccagg agttcacgct
     3481 gtcgactacc gtcttgagcg tatcgaggag tctgataacg agacctacgt tgtccttcgt
     3541 gaggtcgctg tcgctaagta ctctaacctc ggaggaggaa tggatgagct tttcaagtaa
     3601 gagctccgca tcggccgctg tcatcagatc gccatctcgc gcccgtgcct ctgacttcta
     3661 agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt
     3721 tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc
     3781 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
     3841 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
     3901 tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc
     3961 gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt tttatttctt
     4021 cgcacgtctg ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt tttggagtat
     4081 ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct tttgcttttt
     4141 ggggtttccc ctattgtttg tcaagatttc gaggacggcg tttttcttgc taaaatcaca
     4201 agtattgatg agcacgatgc aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg
     4261 aaggtgagta gaagttgata atttgaaagt ggagtagtgt ctatggggtt tttgccttaa
     4321 atgacagaat acattcccaa tataccaaac ataactgttt ctactagtcg gccgtacggg
     4381 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     4441 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     4501 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     4561 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     4621 atcaggcggc cttaattaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     4681 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4741 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4801 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4861 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4921 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4981 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5041 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5101 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5161 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5221 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5281 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5341 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5401 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5461 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5521 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5581 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5641 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5701 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5761 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5941 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6001 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6181 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6241 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6301 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6361 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6421 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6481 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6541 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6601 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     6661 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     6721 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     6781 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     6841 gaaacagct
//
