LOCUS       sequence-371230-       11556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 Spike_WT in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, 
            Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar
            S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, 
            Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne 
            Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M
  TITLE     Human protein interaction networks of ancestral and variant 
            SARS-CoV-2 in organ-specific cells and bodily fluids.
  JOURNAL   Nat Commun. 2025 Jul 1;16(1):5784. doi: 10.1038/s41467-025-60949-1.
  PUBMED    40593736
REFERENCE   2  (bases 1 to 11556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-1"; volume: "16(1):5784. doi"; pages: " 
            10.1038/s41467-025-60949-1"
COMMENT     Sequence Label: pLD-puro-Cc-SARS-CoV-2_Spike_WT-VA
FEATURES             Location/Qualifiers
     source          1..11556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     761..781
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1070..1273
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1224..1244
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1270..1294
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1325..1349
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5172..5196)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5276..5299
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5336..5359
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5372..5389
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5399..5419
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5432..5452
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5465..5530
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5578..5695
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5744..6254
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6173..6191
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6276..6875
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6276..6295)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6772..6792
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7003..7236
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7308..7429
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7345..7364)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7399..7418
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7469..7604
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7624..7643)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7625..7643)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7653..7670)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7653..7669)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7662..7684)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7811..8266
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7898..7917)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8108..8129
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8292..8396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8397..8465,8466..9257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8615..8634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9428..10016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9917..9936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10170..10187
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10304..10325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10340..10345,10346..10363,10364..10370)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10378..10394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10383..10405
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10402..10418
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10402..10418
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10437..10457
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10439..10457
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10485..10711
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10712..10892
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10939..11064
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgatcacga gactagcctc gagcggccgc ccccttcacc gagggcctat ttcccatgat
      781 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
      841 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      901 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      961 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     1021 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     1081 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     1141 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     1201 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1261 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgtac
     1321 gcgtacaagt ttgtacaaaa aagttggcac catgtttgtt tttcttgttt tattgccact
     1381 agtctctagt cagtgtgtta atcttacaac cagaactcaa ttaccccctg catacactaa
     1441 ttctttcaca cgtggtgttt attaccctga caaagttttc agatcctcag ttttacattc
     1501 aactcaggac ttgttcttac ctttcttttc caatgttact tggttccatg ctatacatgt
     1561 ctctgggacc aatggtacta agaggtttga taaccctgtc ctaccattta atgatggtgt
     1621 ttattttgct tccactgaga agtctaacat aataagaggc tggatttttg gtactacttt
     1681 agattcgaag acccagtccc tacttattgt taataacgct actaatgttg ttattaaagt
     1741 ctgtgaattt caattttgta atgatccatt tttgggtgtt tattaccaca aaaacaacaa
     1801 aagttggatg gaaagtgagt tcagagttta ttctagtgcg aataattgca cttttgaata
     1861 tgtctctcag ccttttctta tggaccttga aggaaaacag ggtaatttca aaaatcttag
     1921 ggaatttgtg tttaagaata ttgatggtta ttttaaaata tattctaagc acacgcctat
     1981 taatttagtg cgtgatctcc ctcagggttt ttcggcttta gaaccattgg tagatttgcc
     2041 aataggtatt aacatcacta ggtttcaaac tttacttgct ttacatagaa gttatttgac
     2101 tcctggtgat tcttcttcag gttggacagc tggtgctgca gcttattatg tgggttatct
     2161 tcaacctagg acttttctat taaaatataa tgaaaatgga accattacag atgctgtaga
     2221 ctgtgcactt gaccctctct cagaaacaaa gtgtacgttg aaatccttca ctgtagaaaa
     2281 aggaatctat caaacttcta actttagagt ccaaccaaca gaatctattg ttagatttcc
     2341 taatattaca aacttgtgcc cttttggtga agtttttaac gccaccagat ttgcatctgt
     2401 ttatgcttgg aacaggaaga gaatcagcaa ctgtgttgct gattattctg tcctatataa
     2461 ttccgcatca ttttccactt ttaagtgtta tggagtgtct cctactaaat taaatgatct
     2521 ctgctttact aatgtctatg cagattcatt tgtaattaga ggtgatgaag tcagacaaat
     2581 cgctccaggg caaactggaa agattgctga ttataattat aaattaccag atgattttac
     2641 aggctgcgtt atagcttgga attctaacaa tcttgattct aaggttggtg gtaattataa
     2701 ttacctgtat agattgttta ggaagtctaa tctcaaacct tttgagagag atatttcaac
     2761 tgaaatctat caggccggta gcacaccttg taatggtgtt gaaggtttta attgttactt
     2821 tcctttacaa tcatatggtt tccaacccac taatggtgtt ggttaccaac catacagagt
     2881 agtagtactt tcttttgaac ttctacatgc accagcaact gtttgtggac ctaaaaagtc
     2941 tactaatttg gttaaaaaca aatgtgtcaa tttcaacttc aatggtttaa caggcacagg
     3001 tgttcttact gagtctaaca aaaagtttct gcctttccaa caatttggca gagacattgc
     3061 tgacactact gatgctgtcc gtgatccaca gacacttgag attcttgaca ttacaccatg
     3121 ttcttttggt ggtgtcagtg ttataacacc aggaacaaat acttctaacc aggttgctgt
     3181 tctttatcag gatgttaact gcacagaagt ccctgttgct attcatgcag atcaacttac
     3241 tcctacttgg cgtgtttatt ctacaggttc taatgttttt caaacacgtg caggctgttt
     3301 aataggggct gaacatgtca acaactcata tgagtgtgac atacccattg gtgcaggtat
     3361 atgcgctagt tatcagactc agactaattc tcctcggcgg gcacgtagtg tagctagtca
     3421 atccatcatt gcctacacta tgtcacttgg tgcagaaaat tcagttgctt actctaataa
     3481 ctctattgcc atacccacaa attttactat tagtgttacc acagaaattc taccagtgtc
     3541 tatgaccaag acatcagtag attgtacaat gtacatttgt ggtgattcaa ctgaatgcag
     3601 caatcttttg ttgcaatatg gcagtttttg tacacaatta aaccgtgctt taactggaat
     3661 agctgttgaa caagacaaaa acacccaaga agtttttgca caagtcaaac aaatttacaa
     3721 aacaccacca attaaagatt ttggtggttt taatttttca caaatattac cagatccatc
     3781 aaaaccaagc aagaggtcat ttattgaaga tctacttttc aacaaagtga cacttgcaga
     3841 tgctggcttc atcaaacaat atggtgattg ccttggtgat attgctgcta gagacctcat
     3901 ttgtgcacaa aagtttaacg gccttactgt tttgccacct ttgctcacag atgaaatgat
     3961 tgctcaatac acttctgcac tgttagcggg tacaatcact tctggttgga cctttggtgc
     4021 aggtgctgca ttacaaatac catttgctat gcaaatggct tataggttta atggtattgg
     4081 agttacacag aatgttctct atgagaacca aaaattgatt gccaaccaat ttaatagtgc
     4141 tattggcaaa attcaagact cactttcttc cacagcaagt gcacttggaa aacttcaaga
     4201 tgtggtcaac caaaatgcac aagctttaaa cacgcttgtt aaacaactta gctccaattt
     4261 tggtgcaatt tcaagtgttt taaatgatat cctttcacgt cttgacaaag ttgaggctga
     4321 agtgcaaatt gataggttga tcacaggcag acttcaaagt ttgcagacat atgtgactca
     4381 acaattaatt agagctgcag aaatcagagc ttctgctaat cttgctgcta ctaaaatgtc
     4441 agagtgtgta cttggacaat caaaaagagt tgatttttgt ggaaagggct atcatcttat
     4501 gtccttccct cagtcagcac ctcatggtgt agtcttcttg catgtgactt atgtccctgc
     4561 acaagaaaag aacttcacaa ctgctcctgc catttgtcat gatggaaaag cacactttcc
     4621 tcgtgaaggt gtctttgttt caaatggcac acactggttt gtaacacaaa ggaattttta
     4681 tgaaccacaa atcattacta cagacaacac atttgtgtct ggtaactgtg atgttgtaat
     4741 aggaattgtc aacaacacag tttatgatcc tttgcaacct gaattagact cattcaagga
     4801 ggagttagat aaatatttta agaatcatac atcaccagat gttgatttag gtgacatctc
     4861 tggcattaat gcttcagttg taaacattca aaaagaaatt gaccgcctca atgaggttgc
     4921 caagaattta aatgaatctc tcatcgatct ccaagaactt ggaaagtatg agcagtatat
     4981 aaaatggcca tggtacattt ggctaggttt tatagctggc ttgattgcca tagtaatggt
     5041 gacaattatg ctttgctgta tgaccagttg ctgtagttgt ctcaagggct gttgttcttg
     5101 tggatcctgc tgcaaatttg atgaagacga ctctgagcca gtgctcaaag gagtcaaatt
     5161 acattacaca ttgccaactt tcttgtacaa agtggttgat tctagagcga tcgccagaga
     5221 gctgttcaac ctgctgggcg agaaccagcc ccccgtggtg atcaaggcga tcgcctggag
     5281 ccaccctcag ttcgagaagg gaggaggaag cggcggaggc agcggaggag gaagctggag
     5341 ccacccgcag ttcgagaaag gcggaagcgg ccatcaccat caccatcacc ctgcaggaga
     5401 aaatctgtac ttccagggga gaggaggcag cgagaacctg tactttcagg ggcctgcagg
     5461 aagagactac aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga
     5521 cgatgacaag tagttcgaat tctcgacctc gagacaaatg gcagtattca tccacaattt
     5581 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     5641 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     5701 ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg gggttgcgcc
     5761 ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga
     5821 aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc
     5881 ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc
     5941 gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca
     6001 ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg
     6061 ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg
     6121 tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg
     6181 cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg
     6241 acctctctcc ccagggggat ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     6301 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     6361 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     6421 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     6481 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     6541 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     6601 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     6661 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6721 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6781 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6841 gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc
     6901 cgaccgaaag gagcgcacga ccccatgcat cggtaccttt aagaccaatg acttacaagg
     6961 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     7021 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     7081 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7141 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7201 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     7261 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     7321 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7381 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     7441 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7501 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7561 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     7621 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     7681 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7741 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7801 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7861 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7921 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7981 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8041 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     8101 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     8161 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     8221 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     8281 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8341 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8401 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8461 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8521 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8581 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8641 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8701 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8761 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8821 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8881 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8941 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     9001 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9061 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     9121 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9181 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9241 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9301 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9361 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9421 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9481 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9541 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9601 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9661 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9721 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9781 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9841 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9901 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9961 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10021 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10081 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    10141 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    10201 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    10261 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    10321 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    10381 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
    10441 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
    10501 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
    10561 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
    10621 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
    10681 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
    10741 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    10801 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    10861 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
    10921 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
    10981 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
    11041 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
    11101 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
    11161 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
    11221 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
    11281 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
    11341 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
    11401 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
    11461 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    11521 aagagtggtg cagagagaaa aaagagcagt gggaat
//
