LOCUS       sequence-371280-        9943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express human ACE2 and human TMPRSS2 with selectable markers iRFP670
            and puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Roelle SM, Shukla N, Pham AT, Bruchez AM, Matreyek KA
  TITLE     Expanded ACE2 dependencies of diverse SARS-like coronavirus receptor
            binding domains.
  JOURNAL   PLoS Biol. 2022 Jul 27;20(7):e3001738. doi: 
            10.1371/journal.pbio.3001738.
  PUBMED    35895696
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2022-07-27"; volume: "20(7):e3001738. doi"; pages: " 
            10.1371/journal.pbio.3001738"
COMMENT     Sequence Label: AttB_ACE2-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR
FEATURES             Location/Qualifiers
     source          1..9943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     65..87
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     79..96
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     80..96
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     103..122
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        103..121
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    195..232
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             2708..2761
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    join(4359..4933,4934..4936,4937..4945)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4526..4543)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4753..4772
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4946..5878
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     CDS             6344..6400
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     6894..6914
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7105..7226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7142..7161)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7196..7215
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(7235..7253)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7274..7290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7274..7290)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7287..7309)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7298..7314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7322..7328,7329..7346,7347..7352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7367..7388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7505..7522)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7676..8264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7756..7775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8435..9226,9227..9295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9058..9077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9296..9400)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9426..9881)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9563..9584)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9775..9794
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
       61 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
      121 ggcgaattgg gtaccgggcc ccccctcgag gtcgacgatg taggtcacgg cattccggac
      181 tcagcgctcg agccggcttg tcgacgacgg cggtctccgt cgtcaggatc atccgcagat
      241 tataccgcaa ctacacgcca ccatgtccag ctcctcctgg ctccttctca gccttgttgc
      301 tgtaactgct gctcagtcca ccattgagga acaggccaag acatttttgg acaagtttaa
      361 ccacgaagcc gaagacctgt tctatcaaag ttcacttgct tcttggaatt ataacaccaa
      421 tattactgaa gagaatgtcc aaaacatgaa taatgctggg gacaaatggt ctgccttttt
      481 aaaggaacag tccacacttg cccaaatgta tccactacaa gaaattcaga atctcacagt
      541 caagcttcag ctgcaggctc ttcagcaaaa tgggtcttca gtgctctcag aagacaagag
      601 caaacggttg aacacaattc taaatacaat gagcaccatc tacagtactg gaaaagtttg
      661 taacccagat aatccacaag aatgcttatt acttgaacca ggtttgaatg aaataatggc
      721 aaacagttta gactacaatg agaggctctg ggcttgggaa agctggagat ctgaggtcgg
      781 caagcagctg aggccattat atgaagagta tgtggtcttg aaaaatgaga tggcaagagc
      841 aaatcattat gaggactatg gggattattg gagaggagac tatgaagtaa atggggtaga
      901 tggctatgac tacagccgcg gccagttgat tgaagatgtg gaacatacct ttgaagagat
      961 taaaccatta tatgaacatc ttcatgccta tgtgagggca aagttgatga atgcctatcc
     1021 ttcctatatc agtccaattg gatgcctccc tgctcatttg cttggtgata tgtggggtag
     1081 attttggaca aatctgtact ctttgacagt tccctttgga cagaaaccaa acatagatgt
     1141 tactgatgca atggtggacc aggcctggga tgcacagaga atattcaagg aggccgagaa
     1201 gttctttgta tctgttggtc ttcctaatat gactcaagga ttctgggaaa attccatgct
     1261 aacggaccca ggaaatgttc agaaagcagt ctgccatccc acagcttggg acctggggaa
     1321 gggcgacttc aggatcctta tgtgcacaaa ggtgacaatg gacgacttcc tgacagctca
     1381 tcatgagatg gggcatatcc agtatgatat ggcatatgct gcacaacctt ttctgctaag
     1441 aaatggagct aatgaaggat tccatgaagc tgttggggaa atcatgtcac tttctgcagc
     1501 cacacctaag catttaaaat ccattggtct tctgtcaccc gattttcaag aagacaatga
     1561 aacagaaata aacttcctgc tcaaacaagc actcacgatt gttgggactc tgccatttac
     1621 ttacatgtta gagaagtgga ggtggatggt ctttaaaggg gaaattccca aagaccagtg
     1681 gatgaaaaag tggtgggaga tgaagcgaga gatagttggg gtggtggaac ctgtgcccca
     1741 tgatgaaaca tactgtgacc ccgcatctct gttccatgtt tctaatgatt actcattcat
     1801 tcgatattac acaaggaccc tttaccaatt ccagtttcaa gaagcacttt gtcaagcagc
     1861 taaacatgaa ggccctctgc acaaatgtga catctcaaac tctacagaag ctggacagaa
     1921 actgttcaat atgctgaggc ttggaaaatc agaaccctgg accctagcat tggaaaatgt
     1981 tgtaggagca aagaacatga atgtaaggcc actgctcaac tactttgagc ccttatttac
     2041 ctggctgaaa gaccagaaca agaattcttt tgtgggatgg agtaccgact ggagtccata
     2101 tgcagaccaa agcatcaaag tgaggataag cctaaaatca gctcttggag ataaagcata
     2161 tgaatggaac gacaatgaaa tgtacctgtt ccgatcatct gttgcatatg ctatgaggca
     2221 gtacttttta aaagtaaaaa atcagatgat tctttttggg gaggaggatg tgcgagtggc
     2281 taatttgaaa ccaagaatct cctttaattt ctttgtcact gcacctaaaa atgtgtctga
     2341 tatcattcct agaactgaag ttgaaaaggc catcaggatg tcccggagcc gtatcaatga
     2401 tgctttccgt ctgaatgaca acagcctaga gtttctgggg atacagccaa cacttggacc
     2461 tcctaaccag ccccctgttt ccatatggct gattgttttt ggagttgtga tgggagtgat
     2521 agtggttggc attgtcatcc tgatcttcac tgggatcaga gatcggaaga agaaaaataa
     2581 agcaagaagt ggagaaaatc cttatgcctc catcgatatt agcaaaggag aaaataatcc
     2641 aggattccaa aacactgatg atgttcagac ctcctttggt accgagacct cccaggtggc
     2701 gcccgctgag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc
     2761 agctttgaac tcagggtcac caccagctat tggaccttac tatgaaaacc atggatacca
     2821 accggaaaac ccctatcccg cacagcccac tgtggtcccc actgtctacg aggtgcatcc
     2881 ggctcagtac tacccgtccc ccgtgcccca gtacgccccg agggtcctga cgcaggcttc
     2941 caaccccgtc gtctgcacgc agcccaaatc cccatccggg acagtgtgca cctcaaagac
     3001 taagaaagca ctgtgcatca ccttgaccct ggggaccttc ctcgtgggag ctgcgctggc
     3061 cgctggccta ctctggaagt tcatgggcag caagtgctcc aactctggga tagagtgcga
     3121 ctcctcaggt acctgcatca acccctctaa ctggtgtgat ggcgtgtcac actgccccgg
     3181 cggggaggac gagaatcggt gtgttcgcct ctacggacca aacttcatcc ttcaggtgta
     3241 ctcatctcag aggaagtcct ggcaccctgt gtgccaagac gactggaacg agaactacgg
     3301 gcgggcggcc tgcagggaca tgggctataa gaataatttt tactctagcc aaggaatagt
     3361 ggatgacagc ggatccacca gctttatgaa actgaacaca agtgccggca atgtcgatat
     3421 ctataaaaaa ctgtaccaca gtgatgcctg ttcttcaaaa gcagtggttt ctttacgctg
     3481 tatagcctgc ggggtcaact tgaactcaag ccgccagagc aggatcgtgg gcggcgagag
     3541 cgcgctcccg ggggcctggc cctggcaggt cagcctgcac gtccagaacg tccacgtgtg
     3601 cggaggctcc atcatcaccc ccgagtggat cgtgacagcc gcccactgcg tggaaaaacc
     3661 tcttaacaat ccatggcatt ggacggcatt tgcggggatt ttgagacaat ctttcatgtt
     3721 ctatggagcc ggataccaag tagaaaaagt gatttctcat ccaaattatg actccaagac
     3781 caagaacaat gacattgcgc tgatgaagct gcagaagcct ctgactttca acgacctagt
     3841 gaaaccagtg tgtctgccca acccaggcat gatgctgcag ccagaacagc tctgctggat
     3901 ttccgggtgg ggggccaccg aggagaaagg gaagacctca gaagtgctga acgctgccaa
     3961 ggtgcttctc attgagacac agagatgcaa cagcagatat gtctatgaca acctgatcac
     4021 accagccatg atctgtgccg gcttcctgca ggggaacgtc gattcttgcc agggtgacag
     4081 tggagggcct ctggtcactt cgaagaacaa tatctggtgg ctgatagggg atacaagctg
     4141 gggttctggc tgtgccaaag cttacagacc aggagtgtac gggaatgtga tggtattcac
     4201 ggactggatt tatcgacaaa tgagggcaga cggctagtaa cttaagaatt caccggtctg
     4261 acctccttct ccctctcttc aggtctgcag tcgacggtac cgcgggcccg ggatccaccg
     4321 gatctagata actgatcact gtggaatcag agagactgcc cctctccctc tcccccccct
     4381 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     4441 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     4501 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     4561 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     4621 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     4681 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     4741 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     4801 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     4861 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     4921 acacgatgat aatatggcca caaccatggc gcgtaaggtc gatctcacct cctgcgatcg
     4981 cgagccgatc cacatccccg gcagcattca gccgtgcggc tgcctgctag cctgcgacgc
     5041 gcaggcggtg cggatcacgc gcattacgga aaatgccggc gcgttctttg gacgcgaaac
     5101 tccgcgggtc ggtgagctac tcgccgatta cttcggcgag accgaagccc atgcgctgcg
     5161 caacgcactg gcgcagtcct ccgatccaaa gcgaccggcg ctgatcttcg gttggcgcga
     5221 cggcctgacc ggccgcacct tcgacatctc actgcatcgc catgacggta catcgatcat
     5281 cgagttcgag cctgcggcgg ccgaacaggc cgacaatccg ctgcggctga cgcggcagat
     5341 catcgcgcgc accaaagaac tgaagtcgct cgaagagatg gccgcacggg tgccgcgcta
     5401 tctgcaggcg atgctcggct atcaccgcgt gatgttgtac cgcttcgcgg acgacggctc
     5461 cgggatggtg atcggcgagg cgaagcgcag cgacctcgag agctttctcg gtcagcactt
     5521 tccggcgtcg ctggtcccgc agcaggcgcg gctactgtac ttgaagaacg cgatccgcgt
     5581 ggtctcggat tcgcgcggca tcagcagccg gatcgtgccc gagcacgacg cctccggcgc
     5641 cgcgctcgat ctgtcgttcg cgcacctgcg cagcatctcg ccctgccatc tcgaatttct
     5701 gcggaacatg ggcgtcagcg cctcgatgtc gctgtcgatc atcattgacg gcacgctatg
     5761 gggattgatc atctgtcatc attacgagcc gcgtgccgtg ccgatggcgc agcgcgtcgc
     5821 ggccgaaatg ttcgccgact tcttatcgct gcacttcacc gccgcccacc accaacgctc
     5881 caccggcggc tactcagatc tcgagctcaa gcttcgaatt ctgatgtcgg gacgcggcaa
     5941 gcagggaggc aaagctcgcg ccaaagccaa gacccgctct tctcgtgccg gtctccagtt
     6001 ccccgtgggc cgagtgcacc gactgctccg caagggcaac tatgctgagc gggtcggggc
     6061 cggcgcgccg gtgtacctgg cggcggtgct ggagtacctg actgccgaga tcctggagct
     6121 ggcgggcaac gccgcccgcg acaacaagaa gacccgcatt atcccgcgcc acttgcagct
     6181 ggccatccgc aacgacgagg agctcaacaa gctgctgggc aaagtaacca tcgctcaggg
     6241 tggtgtcctg cccaacatcc aggctgtgct actgcccaag aagaccgaga gtcaccacaa
     6301 ggccaaaggc aaacgggatc caccggatct agataacagc ggagctacta acttcagcct
     6361 gctgaagcag gctggagacg tggaggagaa ccctggacct accgagtaca agcccacggt
     6421 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     6481 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     6541 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6601 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6661 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6721 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6781 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6841 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     6901 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6961 gcgcacctgg tgcatgaccc gcaagtaata gggatcctga tcataatcag ccataccaca
     7021 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     7081 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     7141 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7201 ttgtccaaac tcatcaatgt atcttaggag atccccttta gtgagggtta atttcgagct
     7261 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7321 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7381 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7441 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7501 cttcctcgct cactgactcg ctgcactcgg tcgttcggct gcggcgagcg gtatcagctc
     7561 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7621 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7681 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7741 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7801 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7861 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7921 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7981 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8041 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8101 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8161 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8221 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8281 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8341 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8401 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8461 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8521 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8581 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8641 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8701 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8761 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8821 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8881 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8941 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9001 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9061 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9121 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9181 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9241 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9301 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9361 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9421 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     9481 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     9541 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     9601 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     9661 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     9721 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     9781 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     9841 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     9901 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg cta
//
