LOCUS       sequence-371286-       18126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian cell expression of FLAG-HUWE1 Y355G/H356G, delta3291-3298.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18126)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 18126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDARMO_CMVT_3xFLAG-HUWE1_YH/ GG_delta3291-3298
FEATURES             Location/Qualifiers
     source          1..18126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..224
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(288..876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(368..387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(915..1706,1707..1775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1538..1557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1776..1847)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  1872..2037
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     enhancer        2131..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3140..3159
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      3212..3221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3224..3289
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3308..3331
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(16553..16573)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(16971..16982)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(17049..17066)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    17055..17262
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        join(17310..17315,17316..17332,17333..17338)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             17527..18060
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gaatatagtc tttacatgcc cttggccacg cccctcttta atacgacggg caatttgcac
       61 ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt tttcacagca
      121 taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta ttgtcatagt
      181 ttagatctat tttgttcagt ttaagacttt attgtccgcc cacacccgct tacgcagggc
      241 atctgcgcat ccacaggaag ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      721 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      901 gggtctgacg cttattacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1021 ttaccatctg gccccagtgc tgcaatgata ccgcgtgacc cacgctcacc ggctccagat
     1081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1201 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1501 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttaggaccca
     1861 agtcaaccgg ttgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt
     1921 ttaaggatta tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc
     1981 taatacgaaa attaaaaatg acaaaatagt ttggaactag atttcactta tctggttcag
     2041 cctgcggtcc ggattcgccc ttaagctagc tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2401 gtacatctac gtattagtca ttgctattac catggtgatg cggttttggc agtacatcaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2581 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     2641 tttagtgaac cgtcagatcc tcactctctt ccgcctcgct gtctgcgagg gccagctgtt
     2701 gggctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga tcggaaaccc
     2761 gtcggcctcc gaacggtact ccgccaccga gggacctgag cgagtccgca tcgaccggat
     2821 cggaaaacct ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg
     2881 tggcgggcgg cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt
     2941 aattaaagta ggcggtgttg acaccgcgga tggtcgaggt gaggtgtggc aggcttgaga
     3001 tccagctgtt ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg
     3061 tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg
     3121 agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca ggtccaaagt
     3181 tggccaggcc tagttggcca ggcctagtgc cgccaccatg ggtgactaca aagaccatga
     3241 cggtgattat aaagatcatg acattgatta caaggatgac gatgacaagg gcggtggtag
     3301 cggtgggctg gaagttctgt tccaggggcc cgtcgacatg aaagtagaca ggactaaact
     3361 gaagaagaca cctactgagg ctcctgcaga ctgcagagcc ttaatagaca aactcaaagt
     3421 ttgtaatgat gagcaacttc tcttggaact gcagcagatc aaaacatgga acattggaaa
     3481 gtgcgagtta tatcactggg tggacctgtt ggaccgcttc gatggaatac tggcagatgc
     3541 tggacagaca gtggagaata tgtcatggat gctcgtatgt gataggccag aaagagagca
     3601 actgaaaatg cttctcttgg ctgtgttgaa cttcacagcc ttgctcattg agtacagctt
     3661 ttcccggcat ctgtacagtt ccatagagca tttgacaact ttattggctt cctctgatat
     3721 gcaagtggtg ctggcagtcc tcaatctcct atatgtattt agcaaaagat caaactacat
     3781 cactcgtctg ggatctgaca agaggacccc gctgctaact cggctacaac atttggcaga
     3841 gagctggggt ggaaaggaga atggctttgg acttgcagaa tgttgcagag acttgcatat
     3901 gatgaaatat ccacccagtg caactacact acactttgaa ttctatgcag atcctggggc
     3961 cgaggtcaaa attgagaaaa ggacaactag taacacacta cattatattc acatagagca
     4021 acttgacaag atttcagaaa gcccttctga aatcatggaa tctcttacca aaatgtacag
     4081 cattcctaag gataagcaga tgctgttatt tacacacata cgactggccc atggcttttc
     4141 taatcacagg aagcgattgc aggcagttca ggccagactg catgcaatat ctatattagt
     4201 gtattccaat gccttgcagg aatcagcaaa cagtatcttg tataatggct tgatagagga
     4261 gttagtggac gtgctgcaaa taacggataa gcagcttatg gagattaaag cagcttcttt
     4321 acgaacatta acatcaattg tccacttgga gagaactccc aaactcagca gtattattga
     4381 ctgtactgga actgcctccg gtggcggatt tttgccagtg cttgtaagga actgtatcca
     4441 ggccatgatt gatccttcca tggatccata ccctcaccag tttgccactg ctctcttctc
     4501 ttttttatac catctggcca gctacgatgc tggtggtgaa gccttggtct cctgtggaat
     4561 gatggaagcc ttattgaagg tcataaagtt tcttggcgat gaacaggacc agataacatt
     4621 tgtcaccaga gccgtcagag tggttgacct tatcaccaac ctggatatgg cagcttttca
     4681 atcccatagt ggactttcta tcttcattta tagacttgag catgaagtag atctgtgccg
     4741 aaaagaatgt ccgtttgtga tcaagccaaa gatccagaga cccaatacta cacaagaagg
     4801 agaggaaatg gaaactgata tggatggagt ccagtgtatt ccacaacgag cagcacttct
     4861 gaaatccatg ttgaatttcc tcaagaaggc catccaagac cctgctttct cagatggcat
     4921 acgacatgtg atggatggtt ctctgcctac ctccctgaaa cacatcatca gcaatgcaga
     4981 atactatggc ccatcactct tcctcctagc tactgaagtg gtgactgtgt ttgtatttca
     5041 agaaccatca ctgctctcct cactccagga caatggattg acagatgtca tgctgcatgc
     5101 actgcttatc aaagatgttc ctgctacccg tgaagtcctt ggctccctcc caaatgtatt
     5161 cagtgcactc tgtttgaatg cccgaggtct tcagtctttt gttcagtgtc agccttttga
     5221 acgcctcttc aaagttcttc tgtctccaga ttacctccca gccatgcgga ggaggagaag
     5281 ttctgatccc cttggggata ctgcatccaa cctggggagt gctgtcgatg agctcatgag
     5341 acatcagccc acccttaaaa cagatgcaac gactgccatc atcaagttac ttgaagaaat
     5401 ctgtaatctt ggaagggacc ccaaatacat ctgtcagaag ccatcgatcc agaaggcaga
     5461 tggcactgcc actgctcctc ccccaaggtc taatcatgcc gcagaagaag cctctagtga
     5521 ggatgaggag gaagaggaag tacaggccat gcagagcttt aattctaccc agcaaaatga
     5581 aactgagcct aatcagcagg ttgttggtac agaggaacgt attcctattc ccctcatgga
     5641 ttacatcctt aatgtgatga aatttgtgga atctattctg agcaacaata caacagatga
     5701 ccactgccag gaatttgtga atcagaaagg actgttgcct ttggttacca ttttgggtct
     5761 tcccaatctg cccattgact ttcccacatc tgctgcctgt caggctgttg caggtgtctg
     5821 caaatccata ttgacactgt cacatgaacc caaagtcctt caagagggtc tccttcagtt
     5881 ggactccatc ctctcctccc tggagccctt acaccgcccc attgaatccc ctgggggctc
     5941 agtgttgttg cgagaactgg cttgcgcagg caatgttgct gatgctaccc tctcagccca
     6001 ggccacacct ctgctgcatg cactcactgc tgcccatgcc tacatcatga tgtttgttca
     6061 tacttgcaga gttggacaga gtgaaattcg ttccatctcc gtaaaccagt ggggctctca
     6121 attgggtctg agtgttttga gcaagctgag ccagttatac tgttccctgg tgtgggaaag
     6181 cactgtcctc ctctctctgt gtaccccaaa cagcctacca tctgggtgtg aatttggcca
     6241 ggcagatatg cagaaactgg ttccaaagga tgagaaggca ggtacgaccc agggcggaaa
     6301 aagatcagat ggggaacagg atggagcagc tggaagtatg gatgcttcta cccagggctt
     6361 attagaaggc attgggctag atggtgacac attggctccc atggagacag atgaacctac
     6421 tgcttcagac tctaagggca aatctaaaat cacaccagca atggctgcca gaattaagca
     6481 aatcaagcct ttgttatcag cttcctccag attaggccga gcacttgctg agctatttgg
     6541 acttcttgtt aaactttgtg tgggatctcc tgtccgccag agaaggagcc atcatgctgc
     6601 cagcaccact acagcaccga cacctgccgc gcgatcaaca gcctcagctc tcactaagct
     6661 cttgactaag gggttatctt ggcagccccc accatataca cctactcccc gattcaggct
     6721 gacattcttc atctgttcag ttggtttcac atccccaatg ctgtttgatg agaggaagta
     6781 tccctaccac ctcatgctgc aaaaatttct ctgctccgga ggccacaatg ctctttttga
     6841 aactttcaac tgggctctgt ccatgggagg taaagttcct gtttctgagg gattggaaca
     6901 ctcagacttg cctgatggca caggagaatt cctagatgcc tggcttatgc tggtggagaa
     6961 gatggtgaat cccaccacgg tgcttgaatc tccacattcg ctgcctgcca aattgcctgg
     7021 aggtgtccag aactttcccc agttcagtgc actgcgcttc cttgtggtaa ctcagaaagc
     7081 agcctttact tgcatcaaaa acttatggaa ccggaaaccc ctgaaggtat atggtggacg
     7141 aatggctgaa tcgatgctgg ccattctatg ccacatcctc cgaggagaac ctgtgattcg
     7201 agagagacta agcaaggaga aggaggggtc tcgaggagaa gaggatacag ggcaagagga
     7261 aggtggctcc cgccgggaac ctcaagtcaa ccagcaacaa ctgcaacagc tcatggacat
     7321 gggcttcaca agggaacatg caatggaggc actgttgaac accagcacca tggagcaggc
     7381 cacagagtac cttttaaccc accctcctcc aatcatggga ggagttgttc gggatctcag
     7441 catgtctgaa gaggaccaga tgatgagagc aattgctatg tctctgggac aggatattcc
     7501 aatggatcaa agggcagagt cacctgagga agttgcttgc cggaaggagg aagaggaacg
     7561 gaaagctcgg gaaaagcagg aggaggaaga ggctaaatgt ctagagaagt tccaggatgc
     7621 tgacccgttg gaacaagatg agctccacac tttcacagat actatgttgc caggctgctt
     7681 ccaccttctt gatgagctgc cagacacagt ataccgtgtg tgtgacctga tcatgacagc
     7741 aatcaaacgt aatggagcag attatcgtga catgattctg aagcaagtag tcaatcaggt
     7801 gtgggaagct gctgatgtat tgatcaaagc tgctcttccc ctgacaacaa gtgacacaaa
     7861 aaccgtgtca gagtggataa gtcagatggc cacactgccc caggcctcca atttggctac
     7921 tagaatcttg cttttaacgc tactttttga ggagttgaag ctaccttgtg cttgggtggt
     7981 tgaatcaagt ggcatcctta atgtcctaat caaactcttg gaagtggttc agccctgcct
     8041 ccaggcagcc aaggagcaga aggaagtcca gaccccaaag tggatcacac cagtgttgct
     8101 cctgattgat ttctatgaaa agacagccat ctcctcaaaa aggagagccc agatgactaa
     8161 gtacctgcaa tccaacagca acaactggcg ctggtttgat gatcgctctg ggcgttggtg
     8221 tagttacagt gcaagcaaca atagcactat tgattctgcc tggaaatctg gagagacaag
     8281 cgtgcgattc actgcaggcc gaagaagata cacggtccaa ttcactacaa tggtgcaggt
     8341 taatgaggaa acagggaacc gacgccctgt gatgctgact ctcctcaggg tacctcggct
     8401 gaataaaaat tcaaaaaaca gcaatggaca ggaactagag aagacgctgg aagaaagcaa
     8461 agaaatggat atcaaacgta aagaaaataa aggcaatgat acccctttgg ccctagagag
     8521 tacaaacact gaaaaggaga caagcctgga ggaaacaaaa atcggggaga tcctgatcca
     8581 gggcttgaca gaagatatgg tgactgtttt aatccgggcc tgcgtgagca tgctgggagt
     8641 ccctgtggac ccagatactt tgcatgccac ccttcgtctc tgtctgaggc tcacccggga
     8701 ccacaaatat gccatgatgt ttgcagaact gaagagtacc cgcatgatct tgaatttgac
     8761 ccagagctca ggcttcaatg ggtttactcc cctggtcacc cttctcttaa gacacatcat
     8821 tgaggacccc tgtacccttc gtcataccat ggaaaaggtt gttcgctcag cagctacaag
     8881 tggagctggt agcactacct ctggtgttgt gtctggcagc ctcggctctc gggagatcaa
     8941 ctacatcctt cgtgtccttg ggccagccgc atgccgcaat ccagacatat tcacagaagt
     9001 ggccaactgc tgtatccgca tcgcccttcc tgcccctcga ggctcaggaa ctgcttcaga
     9061 tgatgaattt gagaatctta gaattaaagg ccctaatgct gtacagctgg tgaagaccac
     9121 ccctttgaag ccctcacctc tgcctgtcat ccctgatact atcaaggaag tgatctatga
     9181 tatgctgaat gctctggctg cataccatgc tccagaggaa gccgataaat ctgatcctaa
     9241 acctggggtt atgacccaag aggttggcca gctcctgcaa gacatgggtg atgatgtata
     9301 ccagcagtac cggtcactta cgcgtcagag cagtgacttt gatacgcagt caggtttttc
     9361 cattaatagt caggtctttg ctgcagatgg tgcctccact gagacttccg catctgggac
     9421 ctcccaagga gaggcttcaa ctccagagga gtctcgagat gggaagaaag ataaagaagg
     9481 ggaccgggcc tctgaggaag gcaaacagaa aggcaagggc agcaaacctt taatgcctac
     9541 ctccactatc cttcgtcttc tggcagagtt ggtgaggtcc tatgttggta ttgctaccct
     9601 gattgccaac tacagctaca ctgtgggcca gtctgaactg atcaaagagg actgcagtgt
     9661 gctagctttt gttctggacc acctgctccc acatacccag aatgcagaag acaaggacac
     9721 ccctgccttg gcccgcctgt tcctcgcaag cctggctgct gcagggagtg gcacagatgc
     9781 ccaggtggcc ctagtgaatg aagtaaaagc agcccttgga cgggcactgg ctatggctga
     9841 gagtacagag aaacatgcca ggcttcaggc agtgatgtgt atcatcagta ctatcatgga
     9901 gtcctgcccc tccacctcca gcttctacag cagtgccaca gcgaagaccc agcacaatgg
     9961 catgaacaac atcattcggc ttttcctgaa gaagggactg gttaatgacc tggccagagt
    10021 acctcacagc ttagacctgt ccagtcccaa catggccaac acagtcaatg ctgctctgaa
    10081 gcctttggaa acactttccc ggattgtgaa ccagcccagt agcctttttg gcagcaagag
    10141 tgcttctagc aagaacaagt ctgagcagga tgcccaagga gcctctcaag attccagtag
    10201 caaccagcag gacccaggcg agcctgggga agcagaagtg caggaggagg atcatgatgt
    10261 cactcagaca gaggtggcag atggggatat catggatggg gaggctgaaa ccgactcagt
    10321 ggtgattgct gggcagcctg aggtgctcag ttcacaagag atgcaggttg agaatgagct
    10381 ggaggacctg atagatgagt tgcttgagag ggatggcgga tctgggaaca gtacaattat
    10441 agtgagcaga agtggagagg atgaatcaca agaggacgtg ctgatggatg aagctccttc
    10501 caacctcagc caagcttcca ccttgcaggc caaccgagaa gattccatga atatcctgga
    10561 ccctgaggat gaggaggagc acactcagga agaggacagc agtggcagta acgaggatga
    10621 ggatgatagt caggatgaag aggaggagga ggaggaagat gaggaagatg atcaggagga
    10681 tgatgaaggt gaagagggag atgaagacga tgacgacgat ggctctgaga tggaattgga
    10741 tgaggattat cctgatatga acgcttctcc cttggtccga tttgagcgct ttgaccggga
    10801 ggatgatctc atcattgagt ttgacaacat gttctccagt gctacagaca tccccccatc
    10861 cccaggaaat atccctacca cccatccact gatggtgcgc catgcagacc acagttctct
    10921 gacactgggc agtggctctt caacaactcg tctcacccag ggcatcgggc gcagtcagag
    10981 gaccctaagg cagctgacgg ccaatactgg ccacaccatt catgttcact accctgggaa
    11041 tcgccagccc aaccctcctc ttatactgca gaggttgctt ggtccctcag ctgctgctga
    11101 catccttcag ctgagcagca gccttcccct acaaagccgg ggtcgggccc gcctcctggt
    11161 aggcaacgat gacgtccaca tcatcgcccg ttctgatgat gagctgctgg atgacttttt
    11221 ccatgatcag agcacagcta ccagccaagc aggaaccctg tccagcatcc ccacagccct
    11281 gacccgctgg acagaagaat gcaaagttct cgatgctgag agcatgcatg actgtgtttc
    11341 agtggttaaa gtgtccattg tcaatcacct ggaattcctg agggatgagg agctggaaga
    11401 aaggcgagag aagcgcagga aacaactggc tgaggaagaa acaaagataa ctgataaagg
    11461 caaagaagat aaggagaaca gggatcagag tgcacagtgt actgcatcta agtcaaatga
    11521 ctccactgaa cagaatctct cagatgggac gcctatgcct gacagctacc caacaacccc
    11581 atcttcaact gatgcagcta catctgagtc caaggagacc cttggcactc tgcaatcctc
    11641 acaacagcaa ccaacactcc caaccccacc agctttggga gaggttcctc aggagctgca
    11701 gtctccagct ggagaagggg gcagctctac acagctattg atgcctgtag agccagagga
    11761 attgggtccc acaaggccaa gtggggaagc agaaacaact cagatggagt tatccccagc
    11821 tcccactata acctcacttt ccccagagag agctgaggat tctgatgcac tgacggctgt
    11881 cagcagtcag ctagaaggct ctcctatgga tacaagcagc ctggcttcct gtaccttaga
    11941 ggaggctgtg ggtgacactt cagcagctgg cagttctgag cagcccagag caggcagctc
    12001 cactcctggg gatgccccac cagctgtggc ggaagtgcaa ggcaggagtg atgggtcagg
    12061 ggaatctgcc cagccacctg aggacagctc cccacctgca tcctctgaga gctcttccac
    12121 cagagattct gccgtggcca tttctggcgc cgattctcgg ggaatactgg aagagccact
    12181 gccatctacc tctagtgagg aagaagatcc tcttgccggt atatcattgc ccgagggggt
    12241 agacccgtct ttcctcgccg ccctcccgga cgatattcgg agagaagtac ttcaaaatca
    12301 actcggaatt cggcccccca ctcgcactgc cccgagtact aatagctcag cgcccgccgt
    12361 tgtcgggaat ccaggagtca ctgaagtgag tccggagttc cttgcagcac tcccgccggc
    12421 tattcaggaa gaagtccttg cgcaacagcg agcggaacaa cagaggcgag aacttgccca
    12481 aaacgcatct tcagacactc caatggaccc tgtcacgttc atacagaccc ttccctccga
    12541 cttgcgacga tccgtgctgg aagacatgga agattctgta cttgccgtaa tgccgcctga
    12601 tattgcggca gaagcacaag ctcttcgaag ggaacaagaa gcaaggcagc gacagttgat
    12661 gcatgagagg ctcttcggtc actcctcaac ctccgcgttg tctgccatac tcagatcacc
    12721 cgcgttcaca agccgcctct caggaaatcg gggcgtccag tacaccagat tggccgtcca
    12781 acgaggcggc acgtttcaaa tgggcgggtc tagctcacat aataggccaa gcggctcaaa
    12841 tgtagatact ctgctgcgac ttcggggacg actgctgttg gatcacgaag cgctctcctg
    12901 tctcttggtt ctgctcttcg ttgatgagcc taagctcaac acaagtaggc tccatcgcgt
    12961 actgcggaat ttgtgctatc atgcgcagac acgccattgg gtaattagga gcttgctttc
    13021 aatcctgcag cgaagttcag aatccgaact ttgcatagaa acaccaaagc ttaccacttc
    13081 cgaggaaaag gggaaaaagt cttcaaaatc ttgcggtagc agctcacatg aaaatcgccc
    13141 gctcgatctt ttgcacaaaa tggaaagcaa gtctagtaat cagctctcct ggctttccgt
    13201 ttccatggga tctgggaata tatttcagat tcagagatct ggtggccgca agcatacaga
    13261 aaagcatgct tctggtggaa gtacggtaca catacaccct caggcagccc ctgtcgtatg
    13321 caggcacgtg ttggacacac tcatccagct cgctaaggtc tttccctccc attttactca
    13381 gcaacgaacg aaggaaacca attgcgagag tgaccgcgaa agagggaaca aagcgtgctc
    13441 tccgtgttct tcccagtctt caagtagcgg catttgtact gatttttggg atctgcttgt
    13501 gaaactggat aacatgaacg tttccagaaa ggggaaaaac tccgtcaaat ctgtcccggt
    13561 gtctgcggga ggcgagggtg agacatcccc ttactctctg gaggcatcac ccctgggaca
    13621 acttatgaat atgctcagcc accctgtgat acgaagatcc tcacttctga cggaaaagct
    13681 gctcaggctg ctttcactga tttccatagc acttcctgag aacaaggtga gtgaggcaca
    13741 ggcaaattcc gggtctggag cttcttccac aacgactgcg acctccacaa ccagcacaac
    13801 aactacgact gctgcgtcta ccacccctac tccccctacg gcgcctactc ccgtcacatc
    13861 cgcgccggcc ctcgtagccg ctacggcgat aagtacgatc gtcgttgcgg ccagtactac
    13921 ggtcaccaca ccgaccacag cgaccacgac ggtcagtatt agtcctacga ctaaggggtc
    13981 aaagagccct gctaaagtgt ccgacggagg atcctcctca acagatttta aaatggtgtc
    14041 ctctggactt acggaaaacc agctgcagct ttccgtagag gtacttacct cacattcctg
    14101 cagcgaggaa ggactcgaag acgctgcgaa tgtgctcctg cagctgagta gaggagattc
    14161 cggtaccagg gataccgttc tgaaattgct tcttaatggc gccaggcacc tcgggtacac
    14221 tctgtgcaag caaattggta ctctgttggc cgaactccga gaatacaatc tggagcagca
    14281 acgacgggcg cagtgtgaaa cactgagtcc cgacgggctt cctgaggaac agccgcagac
    14341 aacgaaactt aagggtaaaa tgcagagtcg gttcgacatg gcagaaaatg tcgtgatagt
    14401 ggcttctcag aaacgccctc tcgggggaag ggaactgcag ctgcccagta tgtccatgct
    14461 tacttccaag acaagcaccc agaagttttt tttgagagta cttcaagtca taattcaatt
    14521 gagagacgat actcgccgag ccaacaagaa agccaaacaa acgggaaggc tcggttcttc
    14581 tggtctgggc tcagcatcat ccatccaggc ggccgtgcgg caacttgaag cagaggcaga
    14641 cgccatcata caaatggtac gtgagggtca aagggcgcgg agacagcaac aagcagcaac
    14701 gtcggagtct agccagtcag aggcgtctgt ccggagggag gaatcaccca tggatgtgga
    14761 ccagccatct cccagtgctc aagatactca atccattgcc tccgatggaa ccccacaggg
    14821 ggagaaggaa aaggaagaaa gaccacctga gttacccctg ctcagcgagc agctgagttt
    14881 ggacgagctg tgggacatgc ttggggagtg tctaaaggaa ctagaggaat cccatgacca
    14941 gcatgcggtg ctagtgctac agcctgctgt cgaggccttc tttctggtcc atgccacaga
    15001 gcgggagagc aagcctcctg tccgagacac ccgtgagagc cagctggcac acatcaagga
    15061 cgagcctcct ccactctccc ctgccccctt aaccccagcc acgccttcct cccttgaccc
    15121 attcttctcc cgggagccct catctatgca catctcctca agcctgcccc ctgacacaca
    15181 gaagttcctt cgctttgcag agactcaccg cactgtgtta aaccagatcc tacggcagtc
    15241 cacgacccac cttgctgatg ggccttttgc tgtcctggta gactacattc gtgtcctcga
    15301 ctttgatgtc aagcgcaaat atttccgcca agagctggag cgtttagatg aggggctccg
    15361 gaaagaagac atggctgtgc atgtccgtcg tgaccatgtg tttgaagact cctatcgtga
    15421 gctgcatcgc aaatcccccg aagaaatgaa gaatcgattg tatatagtat ttgaaggaga
    15481 agaagggcag gatgctggcg ggctcctgcg ggagtggtat atgatcatct ctcgagagat
    15541 gtttaaccct atgtatgcct tgttccgtac ctcacctggt gatcgagtca cctacaccat
    15601 caatccatct tcccactgca accccaacca tctgtcctat tttaaatttg tcggacgcat
    15661 tgtggccaaa gctgtatatg acaaccgtct tctggagtgc tactttactc gatcctttta
    15721 caaacacatc ttgggcaagt cagtcagata tacagatatg gagagtgaag attaccactt
    15781 ctaccaaggt ctggtttatc tgctggaaaa tgatgtctcc acactaggct atgacctcac
    15841 cttcagcact gaggtccaag agtttggagt ttgtgaagtt cgtgacctca aacccaatgg
    15901 ggccaacatc ttggtaacag aggagaataa gaaggagtat gtacacctgg tatgccagat
    15961 gagaatgaca ggagccatcc gcaagcagtt ggcggctttc ttagaaggct tctatgagat
    16021 cattccaaag cgcctcattt ccatcttcac tgagcaggag ttagagctgc ttatatcagg
    16081 actgcccacc attgacatcg atgatctgaa atccaacact gaataccaca agtaccagtc
    16141 caactctatt cagatccagt ggttctggag agcattgcgt tctttcgatc aagctgaccg
    16201 tgccaagttc ctccagtttg tcacgggtac ttccaaggta cccctgcaag gctttgctgc
    16261 cctcgaaggc atgaatggca ttcagaagtt tcagatccat cgagatgata ggtccacaga
    16321 tcgcctgcct tcagctcaca catgttttaa tcagctggat ctgcctgcct atgagagctt
    16381 tgagaagctc cgccacatgc tactgttggc tatccaggag tgctctgaag gctttgggct
    16441 ggcctaataa gcggccgctg atgataatgg cctgtggccg taatggcctg tggccgtaaa
    16501 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
    16561 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
    16621 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
    16681 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
    16741 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
    16801 ttgccacggc ggaactcagt gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
    16861 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
    16921 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
    16981 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcggcttc
    17041 gagatctgcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
    17101 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
    17161 tgcatcaaat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
    17221 caagggggag gattgggcag acaatagcag gcatgctggg gactcgagtt aagggcgaat
    17281 cgctgggacg cccgaacggt ggctactcgt tgacataagc ctgttcggtt cgtaaactgt
    17341 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
    17401 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc
    17461 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
    17521 gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
    17581 ttaggtggct caagtatggg catcattcgt acatgtaggc tcggccctga ccaagtcaaa
    17641 tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gtgatgtagc cacctactcc
    17701 caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac atttattgcg
    17761 cttgctgcct tcgaccaaga agcagttgtt ggcgctctcg cggcttacgt tctgcccaag
    17821 tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg cgagcaccgg
    17881 aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt
    17941 gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc tctctataca
    18001 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
    18061 caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa attgtcacaa
    18121 cgccgc
//
