LOCUS       sequence-37130-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Werdien D, Peiler G, Ryffel GU
  TITLE     FLP and Cre recombinase function in Xenopus embryos.
  JOURNAL   Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3.
  PUBMED    11376165
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2001 Jun 1. 29(11):E53-3."
COMMENT     Sequence Label: pCMV:GFP(FRT)lacZ
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    143..277
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(180..199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     234..253
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    279..312
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      317..326
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(343..360)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(343..359)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(352..374)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggcaggagac tatacaaata actcgagcct ctagaggatc ataatcagcc ataccacatt
       61 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      121 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      181 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      241 gtccaaactc atcaatgtat cttatcatgt ctggatccga agttcctatt ctctagaaag
      301 tataggaact tcccgggcca ccatggtcgc gagtagcttg gcactggccg tcgttttaca
      361 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      421 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      481 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
      541 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat
      601 gcacggttac gatgcgccca tctacaccaa cgtgacctat cccattacgg tcaatccgcc
      661 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag
      721 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct
      781 gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga
      841 cctgagcgca tttttacgcg c
//
