LOCUS       sequence-371312-       10292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of full length human integrin aIIb.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Lin FY, Li J, Xie Y, Zhu J, Huong Nguyen TT, Zhang Y, Zhu J, 
            Springer TA
  TITLE     A general chemical principle for creating closure-stabilizing 
            integrin inhibitors.
  JOURNAL   Cell. 2022 Sep 15;185(19):3533-3550.e27. doi: 
            10.1016/j.cell.2022.08.008.
  PUBMED    36113427
REFERENCE   2  (bases 1 to 10292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.08"; journalName: "Cell"; date: "2022-09-15- 
            15"; volume: "185"; issue: "19"; pages: "3533-3550.e27"
COMMENT     Sequence Label: pD2529-CAG-aIIb
FEATURES             Location/Qualifiers
     source          1..10292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     73..92
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(178..225)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             complement(651..1250)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(734..754)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1231..1250
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(1260..1759)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1308..1327)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     1701..1724
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     enhancer        1843..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2148..2423
                     /label=chicken beta-actin promoter
     intron          2424..3440
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3364..3386
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3448..3467
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     polyA_signal    8203..8258
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8257..8276)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(8968..9556)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9048..9067)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9642..10292,1..159))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tttcagaaac aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga
       61 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
      121 taatcgcggc ctcgacgttt cccgttggat atggctcatt ttttacttcc tcaccttgtc
      181 gtattatact atgccgatat actatgccga tgattaattg tcgacactgc gggggctctg
      241 tgtggtaagc aggtcttaac ctttttactg ccaatgacgc atgggatacg tcgtggcagt
      301 aaaagggctt aaatgccaac gacgcgtccc atacgttgtt ggcattttaa ttcttctctc
      361 tgcagcggca gcatgtgccg ccgctgcaga gagtttctag cgatgacagc ccctctgggc
      421 aacgagccgg gggggctgtc tttctttatg ttttaaatgc actgacctcc cacattccct
      481 ttttagtaaa atattcagaa ataatttaaa tacatcattg caatgaaaat aaatgttttt
      541 tattaggcag aatccagatg ctcaaggccc ttcataatat cccccagttt agtagttgga
      601 cttagggaac aaaggaacct ttaatagaaa ttggacagca agaaagcgag tcaggcaccg
      661 ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg
      721 gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggatgtctc caggaaggcg
      781 ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc
      841 ttgccctggt ggtcgggcga cacgccgacg gtggccagga accacgcggg ctccttgggc
      901 cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac
      961 tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg
     1021 gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg
     1081 acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg
     1141 acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg
     1201 gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ggtggcggac
     1261 gaaaggcccg gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg
     1321 cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg
     1381 agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc
     1441 tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc
     1501 tgcacgagac tagtgagtcg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc
     1561 ggggcggggg ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca
     1621 aagaacggag ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca
     1681 cttgtgtagc gccaagtgcc cagcggggct gctaaagcgc atgctccaga ctgccttggg
     1741 aaaagcgcct cccctacccg gtagagaaac ttgatctgtc gccgcaattc aaacttcgtg
     1801 aggctccggt gcccgtcagt gacctgctat actctggaga cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
     2161 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
     2221 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc gcgccaggcg
     2281 gggcggggsg gggsgrgggg sggggsgggg sgaggcggag aggtgcggcg gcagccaatc
     2341 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     2401 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
     2461 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     2521 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
     2581 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
     2641 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
     2701 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
     2761 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
     2821 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
     2881 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     2941 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     3001 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggrgggctcg ggggaggggc
     3061 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     3121 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     3181 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     3241 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     3301 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     3361 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     3421 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     3481 ttggcaaaga attcggcttg atcgaagccg tctcagggga gagaccacac ccaagctgtc
     3541 tagagccgcc accatgcctt tgctcctcct gttgcccttg ctctgggctg gcgccctcgc
     3601 gttgaacctg gacccagtgc agctcacctt ctatgcaggc cccaatggca gccagtttgg
     3661 attttcactg gacttccaca aggacagcca tgggagagtg gccatcgtgg tgggcgcccc
     3721 gcggaccctg ggccccagcc aggaggagac gggcggcgtg ttcctgtgcc cctggagggc
     3781 cgagggcggc cagtgcccct cgctgctctt tgacctccgt gatgagaccc gaaatgtagg
     3841 ctcccaaact ttacaaacct tcaaggcccg ccaaggactg ggggcgtcgg tcgtcagctg
     3901 gagcgacgtc attgtggcct gcgccccctg gcagcactgg aacgtcctag aaaagactga
     3961 ggaggctgag aagacgcccg taggtagctg ctttttggct cagccagaga gcggccgccg
     4021 cgccgagtac tccccctgtc gcgggaacac cctgagccgc atttacgtgg aaaatgattt
     4081 tagctgggac aagcgttact gtgaagcggg cttcagctcc gtggtcactc aggccggaga
     4141 gctggtgctt ggggctcctg gcggctatta tttcttaggt ctcctggccc aggctccagt
     4201 tgcggatatt ttctcgagtt accgcccagg catccttttg tggcacgtgt cctcccagag
     4261 cctctccttt gactccagca acccagagta cttcgacggc tactgggggt actcggtggc
     4321 cgtgggcgag ttcgacgggg atctcaacac tacagaatat gtcgtcggtg cccccacttg
     4381 gagctggacc ctgggagcgg tggaaatttt ggattcctac taccagaggc tgcatcggct
     4441 gcgcggagag cagatggcgt cgtattttgg gcattcagtg gctgtcactg acgtcaacgg
     4501 ggatgggagg catgatctgc tggtgggcgc tccactgtat atggagagcc gggcagaccg
     4561 aaaactggcc gaagtggggc gtgtgtattt gttcctgcag ccgcgaggcc cccacgcgct
     4621 gggtgccccc agcctcctgc tgactggcac acagctctat gggcgattcg gctctgccat
     4681 cgcacccctg ggcgacctcg accgggatgg ctacaatgac attgcagtgg ctgcccccta
     4741 cgggggtccc agtggccggg gccaagtgct ggtgttcctg ggtcagagtg aggggctgag
     4801 gtcacgtccc tcccaggtcc tggacagccc cttccccaca ggctctgcct ttggcttctc
     4861 ccttcgaggt gccgtagaca tcgatgacaa cggataccca gacctgatcg tgggagctta
     4921 cggggccaac caggtggctg tgtacagagc tcagccagtg gtgaaggcct ctgtccagct
     4981 actggtgcaa gattcactga atcctgctgt gaagagctgt gtcctacctc agaccaagac
     5041 acccgtgagc tgcttcaaca tccagatgtg tgttggagcc actgggcaca acattcctca
     5101 gaagctatcc ctaaatgccg agctgcagct ggaccggcag aagccccgcc agggccggcg
     5161 ggtgctgctg ctgggctctc aacaggcagg caccaccctg aacctggatc tgggcggaaa
     5221 gcacagcccc atctgccaca ccaccatggc cttccttcga gatgaggcag acttccggga
     5281 caagctgagc cccattgtgc tcagcctcaa tgtgtcccta ccgcccacgg aggctggaat
     5341 ggcccctgct gtcgtgctgc atggagacac ccatgtgcag gagcagacac gaatcgtcct
     5401 ggactgtggg gaagatgacg tatgtgtgcc ccagcttcag ctcactgcca gcgtgacggg
     5461 ctccccgctc ctagttgggg cagataatgt cctggagctg cagatggacg cagccaacga
     5521 gggcgagggg gcctatgaag cagagctggc cgtgcacctg ccccagggcg cccactacat
     5581 gcgggcccta agcaatgtcg agggctttga gagactcatc tgtaatcaga agaaggagaa
     5641 tgagaccagg gtggtgctgt gtgagctggg caaccccatg aagaagaacg cccagatagg
     5701 aatcgcgatg ttggtgagcg tggggaatct ggaagaggct ggggagtctg tgtccttcca
     5761 gctgcagata cggagcaaga acagccagaa tccaaacagc aagattgtgc tgctggacgt
     5821 gccggtccgg gcagaggccc aagtggagct gcgagggaac tcctttccag cctccctggt
     5881 ggtggcagca gaagaaggtg agagggagca gaacagcttg gacagctggg gacccaaagt
     5941 ggagcacacc tatgagctcc acaacaatgg ccctgggact gtgaatggtc ttcacctcag
     6001 catccacctt ccgggacagt cccagccctc cgacctgctc tacatcctgg atatacagcc
     6061 ccaggggggc cttcagtgct tcccacagcc tcctgtcaac cctctcaagg tggactgggg
     6121 gctgcccatc cccagcccct cccccattca cccggcccat cacaagcggg atcgcagaca
     6181 gatcttcctg ccagagcccg agcagccctc gaggcttcag gatccagttc tcgtaagctg
     6241 cgactcggcg ccctgtactg tggtgcagtg tgacctgcag gagatggcgc gcgggcagcg
     6301 ggccatggtc acggtgctgg ccttcctgtg gctgcccagc ctctaccaga ggcctctgga
     6361 tcagtttgtg ctgcagtcgc acgcatggtt caacgtgtcc tccctcccct atgcggtgcc
     6421 cccgctcagc ctgccccgag gggaagctca ggtgtggaca cagctgctcc gggccttgga
     6481 ggagagggcc attccaatct ggtgggtgct ggtgggtgtg ctgggtggcc tgctgctgct
     6541 caccatcctg gtcctggcca tgtggaaggt cggcttcttc aagcggaacc ggccacccct
     6601 ggaagaagat gatgaagagg gggagtgata aaggttgagg tctctaaaag cgtcttcctg
     6661 ttctcatcac atcatatcaa ggttatatac catcaatatt gccacagatg ttacttagcc
     6721 ttttaatatt tctctaattt agtgtatatg caatgatagt tctctgattt ctgagattga
     6781 gtttctcatg tgtaatgatt atttagagtt tctctttcat ctgttcaaat ttttgtctag
     6841 ttttattttt tactgatttg taagacttct ttttataatc tgcatattac aattctcttt
     6901 actggggtgt tgcaaatatt ttctgtcatt ctatggcctg acttttctta atggtttttt
     6961 aattttaaaa ataagtctta atattcatgc aatctaatta acaatctttt ctttgtggtt
     7021 aggactttga gtcataagaa atttttctct acactgaagt catgatggca tgcttctata
     7081 ttattttcta aaagatttaa agttttgcct tctccattta gacttataat tcactggaat
     7141 ttttttgtgt gtatggtatg acatatgggt tcccttttat tttttacata taaatatatt
     7201 tccctgtttt tctaaaaaag aaaaagatca tcattttccc attgtaaaat gccatatttt
     7261 tttcataggt cacttacata tatcaatggg tctgtttctg agctctactc tattttatca
     7321 gcctcactgt ctatccccac acatctcatg ctttgctcta aatcttgata tttagtggaa
     7381 cattctttcc cattttgttc tacaagaata tttttgttat tgtctttggg ctttctatat
     7441 acattttgaa atgaggttga caagttaata acaggcctat tgattggaaa gtttgtcaac
     7501 gaattgtggg tcttttgggg tttgctgccc cttttacgca atgtggatat cctgctttaa
     7561 tgcctttata tgcatgtata caagcaaaac aggcttttac tttctcgcca acttacaagg
     7621 cctttctcag taaacagtat atgacccttt accccgttgc tcggcaacgg cctggtctgt
     7681 gccaagtgtt tgctgacgca acccccactg gttggggctt ggccataggc catcagcgca
     7741 tgcgtggaac ctttgtgtct cctctgccga tccatactgc ggaactccta gccgcttgtt
     7801 ttgctcgcag caggtctgga gcaaacctca tcgggaccga caattctgtc gtactctccc
     7861 gcaagtatac atcgtttcca tggctgctag gctgtgctgc caactggatc ctgcgcggga
     7921 cgtcctttgt ttacgtcccg tcggcgctga atcccgcgga cgacccctcc cggggccgct
     7981 tggggctcta ccgcccgctt ctccgtctgc cgtaccgtcc gaccacgggg cgcacctctc
     8041 tttacgcgga ctccccgtct gtgccttctc atctgccgga ccgtgtgcac ttcgcttcac
     8101 ctctgcacgt cgcatggagg ccaccgtgaa cgcccaccgg aacctgccca aggtcttgca
     8161 taagaggact cttggacttt cagcaatgtc atctcagtgg ctaataaagg aaatttattt
     8221 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     8281 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     8341 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     8401 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     8461 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     8521 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     8581 gatctttgca tttttagaca tttagaagcc tatatcttgt tacagaattg gaattacaca
     8641 aaaattctac catattttga aagcttaggt tgttctgaaa aaaacaatat attgttttcc
     8701 tgggtaaact aaaagtcccc tcgaggaaag gcccctaaag tgaaacagtg caaaacgttc
     8761 aaaaactgtc tggcaataca agttccactt tgaccaaaac ggctggcagt aaaagggtta
     8821 agaagactgt cagccttgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8881 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8941 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9001 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9061 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9121 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9181 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9241 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9301 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9361 tgctacagag ttcttgaagt ggtgggctaa ctacggctac actagaagaa cagtatttgg
     9421 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9481 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9541 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9601 cgacgcgcgc gtaactcacg ttaagggatt ttggtcatga gttagaaaaa ctcatcgagc
     9661 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     9721 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     9781 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     9841 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     9901 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     9961 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gaggcgaaat
    10021 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg agtgcaaccg gcgcaggaac
    10081 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaac
    10141 gctgtttttc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
    10201 tgcttgatgg tcggaagtgg cataaattcc gtcagccagt ttagtctgac catctcatct
    10261 gtaacatcat tggcaacgct acctttgcca tg
//
