LOCUS       sequence-371330-        9320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding the promoter region of mouse Bmal1 gene 
            driving a luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Gossan N, Zeef L, Hensman J, Hughes A, Bateman JF, Rowley L, Little 
            CB, Piggins HD, Rattray M, Boot-Handford RP, Meng QJ
  TITLE     The circadian clock in murine chondrocytes regulates genes 
            controlling key aspects of cartilage homeostasis.
  JOURNAL   Arthritis Rheum. 2013 Sep;65(9):2334-45. doi: 10.1002/art.38035.
  PUBMED    23896777
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/art"; 
            journalName: "Arthritis Rheum"; date: "2013-09"; volume: "65"; 
            issue: "9"; pages: "2334-45"
COMMENT     Sequence Label: Lentiviral mBmal1-Luc
FEATURES             Location/Qualifiers
     source          1..9320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..43
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    160..181
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(196..201,202..219,220..226)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    234..250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     239..261
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     258..274
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     258..274
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     293..313
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        295..313
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        341..567
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             568..748
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    795..920
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1413..1646
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1831..1875
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2142..2258
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    2487..2535
                     /note="synthetic polyadenylation signal"
     misc_feature    2549..2640
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2589..2608
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      3372..3381
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3378..5027
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             5034..5081
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             5085..5204
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGVDRHPAACASARINV"
     polyA_signal    5253..5374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5504..6092
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5557..5577)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5975..5986)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6179..6412
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6484..6605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6645..6780
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6800..6819)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6801..6819)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6829..6846)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6829..6845)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6838..6860)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6987..7442
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7074..7093)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7284..7305
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7468..7572
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7573..7641,7642..8433)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7791..7810)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8604..9192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9093..9112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
       61 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      121 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      181 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      241 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
      301 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
      361 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
      421 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
      481 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
      541 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
      601 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
      661 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
      721 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
      781 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
      841 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
      901 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
      961 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     1021 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     1081 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     1141 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     1201 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     1261 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     1321 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     1381 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     1441 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     1501 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     1561 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     1621 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     1681 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     1741 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     1801 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     1861 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     1921 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     1981 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     2041 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     2101 tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg
     2161 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     2221 aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt gggagttccg
     2281 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     2341 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     2401 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgaa
     2461 ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg
     2521 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
     2581 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
     2641 ggcctaactg gccggtaccg agctcttacg cgtgctagcc cgagggatgg gcgaagagat
     2701 gcggcgtttt ctcagtgttc tgcgagccag gaaggggacg gaggaggagg gagagctgag
     2761 aggcagtcac tagccaggga cgcggctgag gagcagggga cgacggcgag ctcgcagagt
     2821 ccgcaacgca gtggcctcag cgagctttag acctgagggg gaaaaccgag ggctggggca
     2881 agaaatccac agagcgtgcc aattggtcca ctcctcgggg cgtgtgcttc tgtgcgccaa
     2941 atgattggtg aagggaaagt agcaggtaaa ccagccctgc cgtcttgcca ttggtcagag
     3001 gctttcctat cggtcactcg attggctagc ctaacgcaga gcagaacgcg aattggtttg
     3061 ggttgtccgc caagacaact ccgttcgctc tctctgattg gctaacggga agaggcaggt
     3121 atccgggctg cgcggctcct ccattggtgg gcggggaagg ggggttgggc acagcgattg
     3181 gtgggcgggg ggccgggcct gggccggcgg ggagcggatt ggtcggaaag taggttagtg
     3241 gtgcgacatt tagggaaggc agaaagtagg tcagggacgg aggtgcctgt ttacccgcgc
     3301 cggacttgcc gccgccgccg ccgcgggatc tgcgatctaa gtaagcttgg caatccggta
     3361 ctgttggtaa agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct
     3421 acccactcga agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc
     3481 tggtgcccgg caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg
     3541 agtacttcga gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa
     3601 accatcggat cgtggtgtgc agcgagaata gcctgcagtt cttcatgccc gtgttgggtg
     3661 ccctgttcat cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc
     3721 tgaacagcat gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa
     3781 agatcctcaa cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca
     3841 agaccgacta ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg
     3901 gcttcaacga gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga
     3961 tcatgaacag tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg
     4021 cttgtgtccg attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca
     4081 ccgctatcct cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct
     4141 acttgatctg cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc
     4201 gcagcttgca agactataag attcaatctg ccctgctggt gcccacacta tttagcttct
     4261 tcgctaagag cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg
     4321 gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag
     4381 gcatccgcca gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag
     4441 gggacgacaa gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg
     4501 acttggacac cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc
     4561 ccatgatcat gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg
     4621 acggctggct gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg
     4681 tggaccggct gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg
     4741 agagcatcct gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg
     4801 acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg
     4861 agaaggagat cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg
     4921 gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga
     4981 tccgcgagat tctcattaag gccaagaagg gcggcaagat cgccgtgaat tctgcttgca
     5041 agaactggtt cagtagctta agccactttg tgatccacct taacagccac ggcttccctc
     5101 ccgaggtgga ggagcaggcc gccggcaccc tgcccatgag ctgcgcccag gagagcggcg
     5161 tggatagaca ccctgctgct tgcgccagcg ccaggatcaa cgtctaactg cagtctagag
     5221 tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     5281 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     5341 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     5401 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     5461 tggtaaaatc gataaggatc cgtttgcgta ttgggcggtc gacaatcaac ctctggatta
     5521 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     5581 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     5641 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     5701 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     5761 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     5821 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     5881 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     5941 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6001 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6061 gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga
     6121 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6181 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6421 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6481 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6541 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6601 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     6661 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6721 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6781 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     6841 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     6901 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     6961 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     7021 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7081 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7141 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7201 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7261 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7321 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7381 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     7441 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7501 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7561 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7621 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7681 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7741 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7801 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7861 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7921 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7981 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8041 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8101 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8161 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8221 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8281 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8341 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8401 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8461 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8521 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8581 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8641 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8701 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8761 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8821 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8881 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8941 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9001 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9061 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9121 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9181 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9241 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9301 tttgagtgag ctgataccgc
//
