LOCUS       sequence-371347-        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression of HPV16 E7 E80A/D81A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Nouel J, White EA
  TITLE     ZER1 Contributes to the Carcinogenic Activity of High-Risk HPV E7 
            Proteins.
  JOURNAL   mBio. 2022 Nov 8:e0203322. doi: 10.1128/mbio.02033-22.
  PUBMED    36346242
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2022 
            Nov 8:e0203322. doi: 10.1128/mbio.02033-22."
COMMENT     Sequence Label: MSCV-neo C-HAonly HPV16 E7 E80A/D81A
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    104..128
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(423..447)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             494..520
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             494..520
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        533..1030
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(568..591)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     965..984
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gcaskawcct ccttttatca gccctcactc cttctctagg cgccggaatt agatctaata
       61 cgactcacta tagggagacc caagctggct agttaagcta tcaacaagtt tgtacaaaaa
      121 agttggcacc atgcatggag atacacctac attgcatgaa tatatgttag atttgcaacc
      181 agagacaact gatctctact gttatgagca attaaatgac agctcagagg aggaggatga
      241 aatagatggt ccagctggac aagcagaacc ggacagagcc cattacaata ttgtaacctt
      301 ttgttgcaag tgtgactcta cgcttcggtt gtgcgtacaa agcacacacg tagacattcg
      361 tactttggca gccctgttaa tgggcacact aggaattgtg tgccccatct gttctcagaa
      421 accaccaact ttcttgtaca aagtggttga tctagagggc ccgcggttcg aactcgagtc
      481 ggccgctgga ggatacccct acgacgtgcc cgactacgcc tagaattcta ccgggtaggg
      541 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
      601 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
      661 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
      721 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
      781 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
      841 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
      901 gggggcgggc tcacgggcgg gctcacgggc ggggcgggcg cccgaaagtc ctccggaggc
      961 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctctct tcctcatctc
     1021 gggcctttcg acctgcagcc agcttaccat gattgaacag atgaatgcac gcagattcct
     1081 cccgaccgct tgggatggag aaggggct
//
