LOCUS       sequence-371348-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression of HPV16 E7 Chimera A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Nouel J, White EA
  TITLE     ZER1 Contributes to the Carcinogenic Activity of High-Risk HPV E7 
            Proteins.
  JOURNAL   mBio. 2022 Nov 8:e0203322. doi: 10.1128/mbio.02033-22.
  PUBMED    36346242
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2022 
            Nov 8:e0203322. doi: 10.1128/mbio.02033-22."
COMMENT     Sequence Label: MSCV-P C-FlagHA HPV16-18 E7 Chimera A
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..28
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    104..128
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(435..459)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             491..514
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             530..556
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             530..556
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(604..627)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1001..1020
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gcccskatct cctttatcca gccctcactc cttctctagg cgccggaatt agatctaata
       61 cgactcacta tagggagacc caagctggct agttaagcta tcaacaagtt tgtacaaaaa
      121 agttggcacc atgcatggag atacacctac attgcatgaa tatatgttag atttgcaacc
      181 agagacaact gatctctact gttatgagca attaaatgac agctcagagg aggaggatga
      241 aatagatgga gttaatcatc aacatttacc agcccgacga gccgaaccac aacgtcacac
      301 aatgttgtgt atgtgttgta agtgtgaagc cagaattgag ctagtagtag aaagctcagc
      361 agacgacctt cgagcattcc agcagctgtt tctgaacacc ctgtcctttg tgtgtccgtg
      421 gtgtgcatcc cagcagccaa ctttcttgta caaagtggtt gatctagagg gcccgcggtt
      481 cgaactcgag gactacaagg acgacgatga caagtcggcc gctggaggat acccctacga
      541 cgtgcccgac tacgcctaga attctaccgg gtaggggagg cgcttttccc aaggcagtct
      601 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
      661 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
      721 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
      781 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
      841 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
      901 gctttctggg ctcagaggct gggaaggggg tgggtccggg ggcgggctca cgggcgggct
      961 cagggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     1021 cgcacgtctg cgcgctgctc ctcttcctct tcatctctgg tccttcgaac tkgcagccca
     1081 agccttacca tgaccggaag
//
