LOCUS       sequence-371349-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression of HPV16 E7 Chimera B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Nouel J, White EA
  TITLE     ZER1 Contributes to the Carcinogenic Activity of High-Risk HPV E7 
            Proteins.
  JOURNAL   mBio. 2022 Nov 8:e0203322. doi: 10.1128/mbio.02033-22.
  PUBMED    36346242
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2022 
            Nov 8:e0203322. doi: 10.1128/mbio.02033-22."
COMMENT     Sequence Label: MSCV-P C-FlagHA HPV16-18 E7 Chimera B
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    102..126
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(430..454)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             486..509
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             525..551
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             525..551
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(599..622)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gcasskawct ctttatcagc cctcactcct tctctaggcg ccggaattag atctaatacg
       61 actcactata gggagaccca agctggctag ttaagctatc aacaagtttg tacaaaaaag
      121 ttggcaccat gcatggacct aaggcaacat tgcaagacat tgtattgcat ttagagcccc
      181 aaaatgaaat tccggttgac cttctatgtc acgagcaatt aagcgactca gaggaagaaa
      241 acgatgaaat agatggtcca gctggacaag cagaaccgga cagagcccat tacaatattg
      301 taaccttttg ttgcaagtgt gactctacgc ttcggttgtg cgtacaaagc acacacgtag
      361 acattcgtac tttggaagac ctgttaatgg gcacactagg aattgtgtgc cccatctgtt
      421 ctcagaaacc accaactttc ttgtacaaag tggttgatct agagggcccg cggttcgaac
      481 tcgaggacta caaggacgac gatgacaagt cggccgctgg aggatacccc tacgacgtgc
      541 ccgactacgc ctagaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
      601 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
      661 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
      721 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
      781 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
      841 gcaatggaag cgggtaggcc tttgggggca gcggccaata gcagctttgc tccttcgctt
      901 tctgggctca gaggctggga agggggtggg tccgggggcg ggctcagggc gggctcaggg
      961 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaagcgcacg
     1021 tctgcgcgct kgtctcttct ctcatctccg tccttcgacc tgcagcycag ccttaccatg
     1081 accgag
//
