LOCUS       sequence-371351-        1131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression of HPV16 E7 Mutant 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Nouel J, White EA
  TITLE     ZER1 Contributes to the Carcinogenic Activity of High-Risk HPV E7 
            Proteins.
  JOURNAL   mBio. 2022 Nov 8:e0203322. doi: 10.1128/mbio.02033-22.
  PUBMED    36346242
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2022 
            Nov 8:e0203322. doi: 10.1128/mbio.02033-22."
COMMENT     Sequence Label: MSCV-P C-FlagHA HPV16 E7 Mutant 1
FEATURES             Location/Qualifiers
     source          1..1131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    102..126
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(421..445)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             477..500
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             516..542
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             516..542
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        555..1052
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(590..613)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     985..1004
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cccskawctc tttatccagc cctcactcct tctctaggcg ccggaattag atctaatacg
       61 actcactata gggagaccca agctggctag ttaagctatc aacaagtttg tacaaaaaag
      121 ttggcaccat gcatggagat acacctacat tgcatgaata tatgttagat ttgcaaccag
      181 agacaactga tctctactgt tatgagcaat taaatgacag ctcagaggag gaggatgaaa
      241 tagatggtcc agctggacaa gcagaaccgg acgcagccgc ttacaatatt gtaacctttt
      301 gttgcaagtg tgactctacg cttcggttgt gcgtacaaag cacagccgta gacattcgta
      361 ctttggcagc cctgttaatg ggcacactag gaattgtgtg ccccatctgt tctcagaaac
      421 caccaacttt cttgtacaaa gtggttgatc tagagggccc gcggttcgaa ctcgaggact
      481 acaaggacga cgatgacaag tcggccgctg gaggataccc ctacgacgtg cccgactacg
      541 cctagaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
      601 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
      661 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
      721 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
      781 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
      841 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
      901 gaggctggga aggggtgggt ccgggggcgg gctcagggcg ggctcagggc ggggcgggcg
      961 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     1021 tgttctcctc ttcctcatct ccggcccttt cgacctgcag cccaagctta accatgaccg
     1081 agtacaagcc cmacgggkgg ggtccttcgt cacccgsgga msgaammgat c
//
