LOCUS       sequence-371352-        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression of HPV16 E7 Mutant 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Nouel J, White EA
  TITLE     ZER1 Contributes to the Carcinogenic Activity of High-Risk HPV E7 
            Proteins.
  JOURNAL   mBio. 2022 Nov 8:e0203322. doi: 10.1128/mbio.02033-22.
  PUBMED    36346242
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2022 
            Nov 8:e0203322. doi: 10.1128/mbio.02033-22."
COMMENT     Sequence Label: MSCV-P C-FlagHA HPV16 E7 Mutant 2
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    107..131
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(426..450)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             482..505
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             521..547
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             521..547
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(595..618)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 carywwwmwy wmctmcaatt mtymgyyctc ctccttctct aggcgccgga attagatcta
       61 atacgactca ctatagggag acccaagctg gctagttaag ctatcaacaa gtttgtacaa
      121 aaaagttggc accatgcatg gagatacacc tacattgcat gaatatatgt tagatttgca
      181 accagagaca actgatctct actgttatga gcaattaaat gacagctcag aggaggagga
      241 tgaaatagat ggtccagctg gacaagcaga accggacaga gcccattaca atattgtaac
      301 cttttgttgc aagtgtgact ctacgcttcg gttgtgcgta caaagcacac acgtagacat
      361 tcgtactttg gaagacctgt taatgggcac actaggaatt gtgtgccccg cctgttctca
      421 ggcagcacca actttcttgt acaaagtggt tgatctagag ggcccgcggt tcgaactcga
      481 ggactacaag gacgacgatg acaagtcggc cgctggagga tacccctacg acgtgcccga
      541 ctacgcctag aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
      601 gctttagcag ccccgctggg casttggcgc tacacaagtg gcctctggcc tcgcacacat
      661 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
      721 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
      781 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
      841 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
      901 gctcagaggc tgggaagggg tgggtccrgg gggcgggctc aggggscgsg ctcatgsgac
      961 ggkgccgskc gayscsaagg ttccttcrga cgtccagcag ttwctgcacg cttcataarg
     1021 cgcaygtctr kcakcgctga tcctctstat cckkcawcct mggggtyctt ttcgaccakc
     1081 agaccccwar tsattgawcc satctgcwsc cgsagtct
//
