LOCUS       sequence-371354-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression of HPV16 E7 E80A/D81A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Nouel J, White EA
  TITLE     ZER1 Contributes to the Carcinogenic Activity of High-Risk HPV E7 
            Proteins.
  JOURNAL   mBio. 2022 Nov 8:e0203322. doi: 10.1128/mbio.02033-22.
  PUBMED    36346242
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2022 
            Nov 8:e0203322. doi: 10.1128/mbio.02033-22."
COMMENT     Sequence Label: MSCV-P C-FlagHA HPV16 E7 E80A/D81A
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    103..127
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(422..446)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             478..501
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             517..543
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             517..543
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(591..614)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gcakktatct cctttatcmg ccctcactcc ttctctaggc gccggaatta gatctaatac
       61 gactcactat agggagaccc aagctggcta gttaagctat caacaagttt gtacaaaaaa
      121 gttggcacca tgcatggaga tacacctaca ttgcatgaat atatgttaga tttgcaacca
      181 gagacaactg atctctactg ttatgagcaa ttaaatgaca gctcagagga ggaggatgaa
      241 atagatggtc cagctggaca agcagaaccg gacagagccc attacaatat tgtaaccttt
      301 tgttgcaagt gtgactctac gcttcggttg tgcgtacaaa gcacacacgt agacattcgt
      361 actttggcag ccctgttaat gggcacacta ggaattgtgt gccccatctg ttctcagaaa
      421 ccaccaactt tcttgtacaa agtggttgat ctagagggcc cgcggttcga actcgaggac
      481 tacaaggacg acgatgacaa gtcggccgct ggaggatacc cctacgacgt gcccgactac
      541 gcctagaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
      601 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
      661 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
      721 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
      781 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
      841 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
      901 agaggctggg aaggggtggg tcccggggcg ggctcagggc gggctcaggg cggggcgggc
      961 gcccgaagtc ctccggagcc ggcattctgc acgcttcaaa gcgcacgtct gcgcgctgct
     1021 ctcctcttcc tcatctccgt ccgttcgacc tkgcagacca gcttaccatg accgagttac
//
