LOCUS       sequence-371364-        9477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector with luciferase gene under SFFV promoter and 
            Zsgreen under Cyclophilin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9477)
  AUTHORS   Mishra T, Sreepadmanabh M, Ramdas P, Sahu AK, Kumar A, Chande A
  TITLE     SARS CoV-2 Nucleoprotein Enhances the Infectivity of Lentiviral 
            Spike Particles.
  JOURNAL   Front Cell Infect Microbiol. 2021 Apr 23;11:663688. doi: 
            10.3389/fcimb.2021.663688. eCollection 2021.
  PUBMED    33968806
REFERENCE   2  (bases 1 to 9477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2021-04-23"; volume: "11:663688. doi"; 
            pages: " 10.3389/fcimb.2021.663688. eCollection 2021"
COMMENT     Sequence Label: pScalps Luciferase Zsgreen
FEATURES             Location/Qualifiers
     source          1..9477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      495..504
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             501..1196
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    1206..1794
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1259..1279)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1677..1688)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2312..2492
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(2563..3151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2643..2662)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3322..4113,4114..4182))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3945..3964
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4183..4287)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4362..4381)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4555..4575)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4560..4889
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4740..4875
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4802..4821
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        4901..5280
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5282..5480
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5435..5455
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5498..5678
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5725..5850
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6347..6580
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6764..6808
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7106..7223
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7360..7767
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             7825..9477
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(7891..7909)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gaattctcga ccttgactgg cggcgcgacc ttgaggcctg cgttcgcctc agttgccccc
       61 tctgtgcaat ggggagacgc gcctcatcgc ttgacaacgg ccgaagagcc gccgcgcttc
      121 cgtctcccgc gtgcgcgcgc catgctgccc acccccgttc cgcactgacc ctcccccgtg
      181 ccccgcgtcc cgtactgccg ccccgccccg agtcccatgc cgcagccacc gcgacggagc
      241 ccgcaggcgg gaacctgcct ccgcgcgtta gcgcgcacgc gcgcctcatg tgtcgtcccc
      301 atcagcgccg gcttccgtct ataggccaga tgcactgtca ctctggcgaa gtcgcagacc
      361 cgattggccg ggacggaggc gcgagaccgg gttgcgggcg gggccgaacg tggtataaaa
      421 ggggcgggag gccaggctcg tgccgttttg cagacgccac cgccgaggaa aaccgtgtac
      481 tattagccac gcgtgccacc atggcccagt ccaagcacgg cctgaccaag gagatgacca
      541 tgaagtaccg catggagggc tgcgtggacg gccacaagtt cgtgatcacc ggcgagggca
      601 tcggctaccc cttcaagggc aagcaggcca tcaacctgtg cgtggtggag ggcggcccct
      661 tgcccttcgc cgaggacatc ttgtccgccg ccttcatgta cggcaaccgc gtgttcaccg
      721 agtaccccca ggacatcgtc gactacttca agaactcctg ccccgccggc tacacctggg
      781 accgctcctt cctgttcgag gacggcgccg tgtgcatctg caacgccgac atcaccgtga
      841 gcgtggagga gaactgcatg taccacgagt ccaagttcta cggcgtgaac ttccccgccg
      901 acggccccgt gatgaagaag atgaccgaca actgggagcc ctcctgcgag aagatcatcc
      961 ccgtgcccaa gcagggcatc ttgaagggcg acgtgagcat gtacctgctg ctgaaggacg
     1021 gtggccgctt gcgctgccag ttcgacaccg tgtacaaggc caagtccgtg ccccgcaaga
     1081 tgcccgactg gcacttcatc cagcacaagc tgacccgcga ggaccgcagc gacgccaaga
     1141 accagaagtg gcacctgacc gagcacgcca tcgcctccgg ctccgccttg ccctgagcga
     1201 tcgctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     1261 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     1321 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     1381 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     1441 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     1501 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     1561 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     1621 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     1681 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     1741 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcttaatc
     1801 gcgtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     1861 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     1921 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     1981 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     2041 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     2101 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     2161 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     2221 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     2281 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     2341 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     2401 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     2461 acccttttag tcagtgtgga aaatctctag cagggcccgt ttcatgtgag caaaaggcca
     2521 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2581 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2641 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2701 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2761 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2821 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2881 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2941 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3001 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3061 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3121 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3181 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3241 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3301 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3361 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3421 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3481 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3541 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3601 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3661 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3721 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3781 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3841 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3901 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3961 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4021 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4081 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4141 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4201 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4261 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     4321 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     4381 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     4441 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     4501 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ctgtggaatg
     4561 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4621 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4681 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4741 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4801 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     4861 gcttttttgg aggcctaggc ttttgcaaaa agctcgcgtt gacattgatt attgactagt
     4921 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4981 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     5041 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     5101 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     5161 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     5221 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     5281 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     5341 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     5401 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     5461 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
     5521 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5581 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5641 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     5701 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     5761 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     5821 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     5881 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     5941 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     6001 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     6061 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     6121 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     6181 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     6241 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     6301 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     6361 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     6421 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     6481 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     6541 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     6601 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     6661 aacagatttg gaatcacacg acctggatgg agtgggacag agaattaaca attacacaag
     6721 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     6781 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     6841 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     6901 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     6961 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     7021 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     7081 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     7141 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     7201 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     7261 taattaactg cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga
     7321 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca
     7381 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     7441 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     7501 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     7561 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     7621 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     7681 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     7741 gtcctccgac agactgagtc gcccgggggt ctagaagcgc tggatccgtt taaacgcggc
     7801 cgcccagcac agtggctcga gaccatggaa gacgccaaaa acataaagaa aggcccggcg
     7861 ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga
     7921 tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact
     7981 tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg
     8041 aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg
     8101 ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt
     8161 gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg
     8221 ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg
     8281 gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta
     8341 cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt
     8401 gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat
     8461 agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt
     8521 ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca
     8581 ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg
     8641 tttctgagga gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc
     8701 tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt
     8761 gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat
     8821 ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca
     8881 cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag
     8941 gttgtggatc tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg
     9001 agaggtccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt
     9061 gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc
     9121 ttcatcgttg accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct
     9181 gaattggaat ccatcttgct ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt
     9241 cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg
     9301 atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg
     9361 cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca
     9421 agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtaa
//
