LOCUS       sequence-371366-       10608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression of Human Ace2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Mishra T, Sreepadmanabh M, Ramdas P, Sahu AK, Kumar A, Chande A
  TITLE     SARS CoV-2 Nucleoprotein Enhances the Infectivity of Lentiviral 
            Spike Particles.
  JOURNAL   Front Cell Infect Microbiol. 2021 Apr 23;11:663688. doi: 
            10.3389/fcimb.2021.663688. eCollection 2021.
  PUBMED    33968806
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2021-04-23"; volume: "11:663688. doi"; 
            pages: " 10.3389/fcimb.2021.663688. eCollection 2021"
COMMENT     Sequence Label: pScalps ACE2 hygro
FEATURES             Location/Qualifiers
     source          1..10608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(64..84)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        69..398
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      249..384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     311..330
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        410..789
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        791..989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     944..964
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1007..1187
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1234..1359
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1856..2089
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2273..2317
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2615..2732
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2869..3276
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6276..7313
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             7292..7300
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    7323..7911
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7376..7396)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7794..7805)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8429..8609
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8680..9268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8760..8779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9439..10230,10231..10299))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10062..10081
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10300..10404)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10479..10498)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgc
       61 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
      121 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
      181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
      241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      301 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
      361 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcgcgttg acattgatta
      421 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      481 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      541 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      601 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      661 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      721 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      781 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      841 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      901 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      961 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     1021 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1081 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1141 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1201 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1261 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1321 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1381 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1441 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1501 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1561 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1621 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1681 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     1741 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1801 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1861 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1921 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1981 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     2041 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     2101 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     2161 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaattaacaa
     2221 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2281 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2341 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2401 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2461 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2521 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2581 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2641 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2701 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2761 cagtttggtt aattaactgc agccccgata aaataaaaga ttttatttag tctccagaaa
     2821 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt
     2881 tgcaaggcat ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca
     2941 tgaaaatagc taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc
     3001 cggggccaag aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg
     3061 gtccccagat atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct
     3121 cccccaagga cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc
     3181 gcttctgttc gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc
     3241 ggcgcgccag tcctccgaca gactgagtcg cccgggggtc tagaagcgct ggatccgttt
     3301 aaacgcggcc gcccagcaca gtggctcgag accatgtcaa gctcttcctg gctccttctc
     3361 agccttgttg ctgtaactgc tgctcagtcc accattgagg aacaggccaa gacatttttg
     3421 gacaagttta accacgaagc cgaagacctg ttctatcaaa gttcacttgc ttcttggaat
     3481 tataacacca atattactga agagaatgtc caaaacatga ataatgctgg ggacaaatgg
     3541 tctgcctttt taaaggaaca gtccacactt gcccaaatgt atccactaca agaaattcag
     3601 aatctcacag tcaagcttca gctgcaggct cttcagcaaa atgggtcttc agtgctctca
     3661 gaagacaaga gcaaacggtt gaacacaatt ctaaatacaa tgagcaccat ctacagtact
     3721 ggaaaagttt gtaacccaga taatccacaa gaatgcttat tacttgaacc aggtttgaat
     3781 gaaataatgg caaacagttt agactacaat gagaggctct gggcttggga aagctggaga
     3841 tctgaggtcg gcaagcagct gaggccatta tatgaagagt atgtggtctt gaaaaatgag
     3901 atggcaagag caaatcatta tgaggactat ggggattatt ggagaggaga ctatgaagta
     3961 aatggggtag atggctatga ctacagccgc ggccagttga ttgaagatgt ggaacatacc
     4021 tttgaagaga ttaaaccatt atatgaacat cttcatgcct atgtgagggc aaagttgatg
     4081 aatgcctatc cttcctatat cagtccaatt ggatgcctcc ctgctcattt gcttggtgat
     4141 atgtggggta gattttggac aaatctgtac tctttgacag ttccctttgg acagaaacca
     4201 aacatagatg ttactgatgc aatggtggac caggcctggg atgcacagag aatattcaag
     4261 gaggccgaga agttctttgt atctgttggt cttcctaata tgactcaagg attctgggaa
     4321 aattccatgc taacggaccc aggaaatgtt cagaaagcag tctgccatcc cacagcttgg
     4381 gacctgggga agggcgactt caggatcctt atgtgcacaa aggtgacaat ggacgacttc
     4441 ctgacagctc atcatgagat ggggcatatc cagtatgata tggcatatgc tgcacaacct
     4501 tttctgctaa gaaatggagc taatgaagga ttccatgaag ctgttgggga aatcatgtca
     4561 ctttctgcag ccacacctaa gcatttaaaa tccattggtc ttctgtcacc cgattttcaa
     4621 gaagacaatg aaacagaaat aaacttcctg ctcaaacaag cactcacgat tgttgggact
     4681 ctgccattta cttacatgtt agagaagtgg aggtggatgg tctttaaagg ggaaattccc
     4741 aaagaccagt ggatgaaaaa gtggtgggag atgaagcgag agatagttgg ggtggtggaa
     4801 cctgtgcccc atgatgaaac atactgtgac cccgcatctc tgttccatgt ttctaatgat
     4861 tactcattca ttcgatatta cacaaggacc ctttaccaat tccagtttca agaagcactt
     4921 tgtcaagcag ctaaacatga aggccctctg cacaaatgtg acatctcaaa ctctacagaa
     4981 gctggacaga aactgttcaa tatgctgagg cttggaaaat cagaaccctg gaccctagca
     5041 ttggaaaatg ttgtaggagc aaagaacatg aatgtaaggc cactgctcaa ctactttgag
     5101 cccttattta cctggctgaa agaccagaac aagaattctt ttgtgggatg gagtaccgac
     5161 tggagtccat atgcagacca aagcatcaaa gtgaggataa gcctaaaatc agctcttgga
     5221 gataaagcat atgaatggaa cgacaatgaa atgtacctgt tccgatcatc tgttgcatat
     5281 gctatgaggc agtacttttt aaaagtaaaa aatcagatga ttctttttgg ggaggaggat
     5341 gtgcgagtgg ctaatttgaa accaagaatc tcctttaatt tctttgtcac tgcacctaaa
     5401 aatgtgtctg atatcattcc tagaactgaa gttgaaaagg ccatcaggat gtcccggagc
     5461 cgtatcaatg atgctttccg tctgaatgac aacagcctag agtttctggg gatacagcca
     5521 acacttggac ctcctaacca gccccctgtt tccatatggc tgattgtttt tggagttgtg
     5581 atgggagtga tagtggttgg cattgtcatc ctgatcttca ctgggatcag agatcggaag
     5641 aagaaaaata aagcaagaag tggagaaaat ccttatgcct ccatcgatat tagcaaagga
     5701 gaaaataatc caggattcca aaacactgat gatgttcaga cctcctttta gggtacctat
     5761 aataactggc cagaattctc gaccttgact ggcggcgcga ccttgaggcc tgcgttcgcc
     5821 tcagttgccc cctctgtgca atggggagac gcgcctcatc gcttgacaac ggccgaagag
     5881 ccgccgcgct tccgtctccc gcgtgcgcgc gccatgctgc ccacccccgt tccgcactga
     5941 ccctcccccg tgccccgcgt cccgtactgc cgccccgccc cgagtcccat gccgcagcca
     6001 ccgcgacgga gcccgcaggc gggaacctgc ctccgcgcgt tagcgcgcac gcgcgcctca
     6061 tgtgtcgtcc ccatcagcgc cggcttccgt ctataggcca gatgcactgt cactctggcg
     6121 aagtcgcaga cccgattggc cgggacggag gcgcgagacc gggttgcggg cggggccgaa
     6181 cgtggtataa aaggggcggg aggccaggct cgtgccgttt tgcagacgcc accgccgagg
     6241 aaaaccgtgt actattagcc acgcgtgccg ccaccatgaa gaagcccgaa ctcaccgcta
     6301 ccagcgttga aaaatttctc atcgagaagt tcgacagtgt gagcgacctg atgcagttgt
     6361 cggagggcga agagagccga gccttcagct tcgatgtcgg cggacgcggc tatgtactgc
     6421 gggtgaatag ctgcgctgat ggcttctaca aagaccgcta cgtgtaccgc cacttcgcca
     6481 gcgctgcact acccatcccc gaagtgttgg acatcggcga gttcagcgag agcctgacat
     6541 actgcatcag tagacgcgcc caaggcgtta ctctccaaga cctccccgaa acagagctgc
     6601 ctgctgtgtt acagcctgtc gccgaagcta tggatgctat tgccgccgcc gacctcagtc
     6661 aaaccagcgg cttcggccca ttcgggcccc aaggcatcgg ccagtacaca acctggcggg
     6721 atttcatttg cgccattgct gatccccatg tctaccactg gcagaccgtg atggacgaca
     6781 ccgtgtccgc cagcgtagct caagccctgg acgaactgat gctgtgggcc gaagactgtc
     6841 ccgaggtgcg ccacctcgtc catgccgact tcggcagcaa caacgtcctg accgacaacg
     6901 gccgcatcac cgccgtaatc gactggtccg aagctatgtt cggggacagt cagtacgagg
     6961 tggccaacat cttcttctgg cggccctggc tggcttgcat ggagcagcag actcgctact
     7021 tcgagcgccg gcatcccgag ctggccggca gccctcgtct gcgagcctac atgctgcgca
     7081 tcggcctgga tcagctctac cagagcctcg tggacggcaa cttcgacgat gctgcctggg
     7141 ctcaaggccg ctgcgatgcc atcgtccgca gcggggccgg caccgtcggt cgcacacaaa
     7201 tcgctcgccg gagcgcagcc gtatggaccg acggctgcgt cgaggtgctg gccgacagcg
     7261 gcaaccgccg gcccagtaca cgaccgcgcg ctaaggaggt aggtcgagtt taagcgatcg
     7321 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7381 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7441 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7501 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7561 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7621 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7681 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     7741 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7801 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7861 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cttaatcgcg
     7921 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     7981 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     8041 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     8101 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     8161 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     8221 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     8281 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     8341 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     8401 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     8461 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8521 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8581 cttttagtca gtgtggaaaa tctctagcag ggcccgtttc atgtgagcaa aaggccagca
     8641 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8701 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8761 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8821 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8881 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8941 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9001 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9061 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9121 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9181 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9241 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9301 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9361 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9421 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9481 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9541 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9601 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9661 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9721 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9781 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9841 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9901 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9961 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10021 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10081 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10141 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10201 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10261 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10321 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10381 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    10441 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
    10501 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    10561 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaag
//
