LOCUS       sequence-371383-       10157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ShDART under control of Lac promoter, original configuration 
            (pre-gRNA terminator and J23119 promoter), Sh_lacZ_alpha_1 gRNA, 
            GmR+sfGFP cargo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10157)
  AUTHORS   Rubin BE, Diamond S, Cress BF, Crits-Christoph A, Lou YC, Borges AL,
            Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R, Smock DCJ, Tang
            K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM,
            Banfield JF, Doudna JA
  TITLE     Species- and site-specific genome editing in complex bacterial 
            communities.
  JOURNAL   Nat Microbiol. 2022 Jan;7(1):34-47. doi: 10.1038/s41564-021-01014-7.
            Epub 2021 Dec 6.
  PUBMED    34873292
REFERENCE   2  (bases 1 to 10157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-021-01014-7"; journalName: "Nat Microbiol"; date: 
            "2022-01"; volume: "7"; issue: "1"; pages: "34-47"
COMMENT     Sequence Label: pBFC0694
FEATURES             Location/Qualifiers
     source          1..10157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(238..243,244..261,262..268)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             269..280
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      5698..5769
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        5783..5817
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
     terminator      6071..6098
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        join(6276..6281,6282..6298,6299..6304)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             6493..7026
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     RBS             7385..7396
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             7403..8116
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      8128..8199
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8215..8242
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     8253..8272
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8253..8271
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     oriT            8555..8663
                     /note="incP origin of transfer"
     promoter        8856..8960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8961..9029,9030..9821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9179..9198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9857..10157,1..88)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 acaacaagcc agggatgtaa cgcactgaga agcccttaga gcctctcaaa gcaattttga
       61 gtgacacagg aacacttaac ggctgacatg ggcaagttta ctcatatata ctttagattg
      121 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
      181 tgaccaaaat cccttaacgt gagttttcgt tccactgaca cctgcccgaa ccatatcttt
      241 acactttatg cttccggctc gtatgttgaa agaggagaaa ggatcaatga acagtcagca
      301 aaatcctgat ttagctgttc atcccttggc aattcctatg gaaggcttac taggagaaag
      361 tgctacaact cttgagaaga atgtaattgc cacacaactc tcagaggaag cccaagtaaa
      421 gctagaggta atccaaagtt tactggaacc ctgcgatcgc acaacttatg ggcaaaagtt
      481 gcgggaagca gcagagaaac taaatgtatc gttgcgaacg gtacaaaggt tggtgaaaaa
      541 ctgggaacaa gatggcttag tcggactcac tcaaacaagt agggctgata aaggaaaaca
      601 ccgcattggt gagttttggg aaaacttcat taccaaaacc tacaaggagg gtaacaaggg
      661 aagtaaacgt atgaccccta aacaagttgc tctcagagtc gaggctaaag cccgtgaatt
      721 aaaagactct aagccgccca attacaaaac cgtgttacgg gtattagcac ccattttgga
      781 aaagcaacaa aaagccaaga gtatccgcag tcctggttgg agaggaacta cgctttcggt
      841 taaaacccgt gaaggaaaag atttatcggt tgattacagt aaccatgttt ggcaatgtga
      901 ccatacccgc gtggatgtgt tgctggtaga tcaacatggt gaaattttaa gtcgtccctg
      961 gctaacaaca gtaattgata cttactctcg ttgcattatg ggtatcaact tgggctttga
     1021 tgcacccagt tctggggtag tagcattagc gttacgccat gcaattctac caaagcgtta
     1081 cggttccgag tacaaactgc attgtgagtg gggaacctat ggaaaaccag aacattttta
     1141 tactgatggc ggtaaagact ttcgctctaa ccacttgagt cagattgggg cgcaattggg
     1201 atttgtctgt catttacgcg atcgcccttc tgaaggtgga gtagtagaac gtcccttcaa
     1261 aacattaaat gaccaactat tttcaacgct tcctgggtac accggatcta atgtgcagga
     1321 acgcccagaa gatgcagaga aggacgcaag acttactttg cgagaactag aacagttact
     1381 tgtgcgttac atcgtagatc gttacaacca aagtattgat gcgcggatgg gcgaccaaac
     1441 gcgctttgag cgttgggaag caggattgcc tacagtgcca gtaccaatac cagaacgaga
     1501 tttggatatt tgtttaatga agcagtcacg gcgcactgtg caaagaggtg gttgtttgca
     1561 gtttcagaat ttaatgtatc ggggggaata tttggcaggt tatgccggag aaactgtcaa
     1621 cttaaggttt gaccccagag acattacaac aattttggtt tatcgccagg aaaacaatca
     1681 ggaagtattt ctgactcgcg ctcacgctca aggtttggag acagagcaac tggcattaga
     1741 tgaggctgag gcagcaagtc gcagactccg taccgcaggg aaaactatca gtaaccaatc
     1801 attattgcaa gaagttgttg accgcgatgc tcttgtcgct accaagaaaa gccgtaagga
     1861 gcgtcaaaaa ttggaacaga ctgttttgcg atctgctgct gttgatgaaa gtaatagaga
     1921 atccttgcct tctcaaatag ttgaaccaga tgaagtggaa tctacagaaa cggttcactc
     1981 tcaatacgaa gatattgagg tgtgggacta tgaacaactt cgtgaagaat atgggtttta
     2041 aacaatgaca gaagctcagg cgatcgccaa gcagttgggt ggggtaaaac cggatgatga
     2101 gtggttacaa gctgaaattg ctcgtcttaa gggtaagagc attgtgcctt tacagcaggt
     2161 aaaaactctc catgattggt tagatggcaa gcgcaaggca agaaaatctt gccgagtagt
     2221 tggggaatcg agaactggca agacagttgc ttgtgatgcc tacagataca ggcacaaacc
     2281 tcagcaggaa gctggacgac ctccaactgt gcctgtcgtt tatattcgac ctcaccaaaa
     2341 atgtggcccc aaggatttgt ttaaaaagat tactgagtac ctcaagtatc gggtaacaaa
     2401 agggactgta tctgattttc gagataggac gatagaagta ctcaagggtt gtggcgtaga
     2461 gatgctaatt attgatgaag ctgaccgtct taagcctgaa acttttgctg atgtgcgaga
     2521 tattgccgaa gatttaggaa ttgctgtggt actggtagga acagaccgtt tggatgcggt
     2581 aattaagcgg gatgagcagg ttctcgaacg ctttcgggcg catcttcgct ttggtaaatt
     2641 gtcgggagag gattttaaga acaccgtaga aatgtgggaa caaatggttt tgaaactgcc
     2701 agtatcttct aatctaaaga gcaaggagat gctacggatt ctcacgtcag caactgaagg
     2761 ctacattggt cgccttgatg agattcttag ggaagctgca attcgttcct tatcaagagg
     2821 attgaagaag attgacaagg ctgttttaca ggaagtagct aaggagtaca aatgatagaa
     2881 gcaccagatg ttaaaccttg gctattcttg attaaaccct atgaagggga aagcctgagc
     2941 cactttcttg gcaggttcag acgtgccaac catttatccg caagtggatt gggtactttg
     3001 gcaggaattg gtgctatagt ggcacgttgg gaaagatttc attttaatcc tcgccctagt
     3061 cagcaagaat tggaagcgat cgcatctgta gtagaagtgg atgctcaaag gttagcccag
     3121 atgttaccgc ctgctggagt gggaatgcag catgagccaa ttcgcttgtg tggggcttgt
     3181 tatgccgagt cgccttgtca ccgaattgaa tggcagtaca agtcggtgtg gaagtgcgat
     3241 cgccatcaac tcaagatttt agcaaagtgt ccaaactgtc aagcaccttt taaaatgcct
     3301 gcgctgtggg aggatgggtg ctgtcacaga tgtaggatgc cgtttgcaga aatggcaaag
     3361 ctacagaagg tttgatgata aaaccagaaa aaggtgtgaa attaactaag tccctgaatt
     3421 gatctggttg tccaaaaaat ttgtgcgatc gcatggcaag attattccta ctattgatgt
     3481 ggcgtgagtc ggataacttg ctctaatggg atcccggtta ataccaatag atcagatatg
     3541 agtaataaac attacgacct ttacctgtgg tattcactgt tggcacgaca acacctttat
     3601 cgcgccaata ctgtagctga cgacgcgaac aattagtgat ttgagccgct tctgtgcttg
     3661 tgtagaattt tccttccata gaaatcatcc tacagaagta gttttttaaa tttacatttt
     3721 ttaaaagcac attatgtatt catgtgatat tattttttta tgagtcaaat aactattcaa
     3781 gctcgactta tttcctttga atcaaaccgc caacaactct ggaagttgat ggcagattta
     3841 aacacgccgt taattaacga actgctttgc cagttaggtc aacaccccga cttcgagaag
     3901 tggcaacaaa agggtaaact cccgtctacc gttgtgagcc agttatgtca acctctcaaa
     3961 actgaccctc gctttgcagg tcagcccagc cgtttatata tgtcggcaat tcatattgtg
     4021 gactacatct acaagtcctg gctggctata cagaaacggc ttcaacagca gctagatgga
     4081 aagacgcgct ggctagaaat gctcaatagc gatgctgaat tagtagaact tagtggtgac
     4141 actttagagg ctattcgtgt caaagctgct gaaattttgg caatagctat gccagcatct
     4201 gagtcagata gcgcttcacc taaagggaaa aaaggtaaaa aggagaaaaa accctcatct
     4261 tctagcccta agcgtagttt atccaagaca ttatttgacg cttaccaaga aacggaagat
     4321 atcaagagcc gtagcgccat cagctacctg ttaaaaaatg gctgcaaact tactgacaaa
     4381 gaagaagatt cagaaaaatt tgctaaacgt cgtcgtcaag ttgaaatcca aattcaaagg
     4441 cttaccgaaa agttaataag tcggatgcct aaaggtcgag atttgaccaa tgctaaatgg
     4501 ttggagacac tcttgactgc tacaaccact gttgctgaag ataacgccca agccaaacgc
     4561 tggcaggata ttctgttaac tcgatcaagt tctctcccat tcccccttgt ttttgaaacc
     4621 aacgaggata tggtttggtc aaagaatcaa aagggtaggc tgtgtgttca cttcaatggc
     4681 ttaagcgatt taatttttga ggtgtactgc ggcaatcgtc aacttcactg gtttcaacgc
     4741 ttcctagaag atcaacagac taaacgcaaa agcaaaaatc agcattctag cggcttgttc
     4801 acactcagaa atggtcatct agtttggctt gaaggtgagg gtaaagggga accttggaat
     4861 cttcaccact tgacccttta ctgctgtgtt gacaatcgct tgtggacaga ggagggaaca
     4921 gaaatcgttc gccaagagaa agcagatgaa attactaaat tcatcacaaa catgaagaag
     4981 aaaagcgatc taagcgatac acagcaagct ttgattcaac gtaaacaatc aacacttact
     5041 cgaataaaca attcctttga gcgtcctagc caaccccttt atcaaggtca atcacacatt
     5101 ttggttggag taagcctggg actagaaaaa cctgccacag tagcagtagt agatgcgatc
     5161 gccaacaaag tcttggctta ccggagtatt aaacaattac ttggcgacaa ttacgaactg
     5221 ctaaatcgcc aaagacgaca acagcagtac ctatctcacg aacgccacaa agcacaaaaa
     5281 aacttctctc ccaatcaatt tggagcatct gagttagggc aacatataga cagattatta
     5341 gctaaagcaa ttgtagcgtt agcgagaacc tacaaagctg gcagtattgt cttgcccaag
     5401 ttaggggata tgcgggaggt tgtccaaagt gaaattcaag ctatagcaga acaaaaattt
     5461 cccggttata ttgaaggtca gcaaaaatat gccaaacagt accgggttaa tgttcatcgg
     5521 tggagctacg gcagattaat tcaaagcatt caaagtaaag cagctcaaac aggaattgtg
     5581 attgaggagg gaaaacaacc tattcgaggt agtccccacg acaaagcaaa ggaattagca
     5641 ctttctgctt acaatctccg cctaactagg cgaagttaag taaggatctc caggcatcaa
     5701 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     5761 aacgctctct actagacaga ccctgacagc tagctcagtc ctaggtataa tgctagcata
     5821 ttaatagcgc cgcaattcat gctgcttgca gcctctgaat tttgttaaat gagggttagt
     5881 ttgactgtat aaatacagtc ttgctttctg accctggtag ctgctcaccc tgatgctgct
     5941 gtcaatagac aggataggtg cgctcccagc aataagggcg cggatgtact gctgtagtgg
     6001 ctactgaatc acccccgatc aagggggaac cctaaatggg ttgaaagtta caacgtcgtg
     6061 actgggaaaa ggctcacctt cgggtgggcc tttctgcgca ggtccaaacc agatgtcaac
     6121 acagctactg tacagtgaca aattatctgt cgtcggtgac agattaatgt cattgtgact
     6181 atttaattgt cgtcgtgacc catcagcgtt gcttaattaa ttgatgacaa attaaatgtc
     6241 atcagccagg ggtccccaat aattacgatt taaatttgac ataagcctgt tcggttcgta
     6301 aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     6361 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttgtacagc
     6421 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     6481 ggagcagcaa cgatgttacg cagcagcaac gatgttacgc agcagggcag tcgccctaaa
     6541 acaaagttag gtggctcaag tatgggcatc attcgcacgt gtaggctcgg ccctgaccaa
     6601 gtcaaatcca tgcgggctgc tcttgatctt ttcggtcgtg agttcggtga cgtagccacc
     6661 tactcccaac atcagccgga ttccgattac ctcgggaact tgctccgtag taagacattc
     6721 atcgcgcttg ctgccttcga ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg
     6781 cccaagtttg agcagccgcg tagtgaaatc tatatctatg atctcgcagt ctccggagag
     6841 caccggaggc agggcattgc caccgcgctc atcaatctcc tcaagcatga ggccaacgcg
     6901 cttggtgctt atgtgatcta cgtgcaagca gattacggtg acgatcccgc agtggctctc
     6961 tatacaaagt tgggcatacg ggaagaagtg atgcactttg atatcgaccc aagtaccgcc
     7021 acctaacaat tcgttcaagc cgagatcggc ttcccggccg cggagttgtt cggtaaattg
     7081 gacaacggtc cgcgcgttgt ccttttccgc tgcataaccc tgcttcgggg tcattatagc
     7141 gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg
     7201 tgtagacttt ccttggtgta tccaacggcg tcagccgaaa gtgaaacgtg atttcatgcg
     7261 tcattttgaa cattttgtaa atcttattta ataatgtgtg cggcaattca catttaattt
     7321 atgaatgttt tcttaacatc gcggcaactc aagaaacggc aggttcggat cttagctact
     7381 agagaaagag gagaaatact agatgcgtaa aggcgaggag ctgttcactg gtgtcgtccc
     7441 tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg
     7501 tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta ctggtaaact
     7561 gccggttcct tggccgactc tggtaacgac gctgacttat ggtgttcagt gctttgctcg
     7621 ttatccggac catatgaagc agcatgactt cttcaagtcc gccatgccgg aaggctatgt
     7681 gcaggaacgc acgatttcct ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa
     7741 atttgaaggc gataccctgg taaaccgcat tgagctgaaa ggcattgact ttaaagagga
     7801 cggcaatatc ctgggccata agctggaata caattttaac agccacaatg tttacatcac
     7861 cgccgataaa caaaaaaatg gcattaaagc gaattttaaa attcgccaca acgtggagga
     7921 tggcagcgtg cagctggctg atcactacca gcaaaacact ccaatcggtg atggtcctgt
     7981 tctgctgcca gacaatcact atctgagcac gcaaagcgtt ctgtctaaag atccgaacga
     8041 gaaacgcgat catatggttc tgctggagtt cgtaaccgca gcgggcatca cgcatggtat
     8101 ggatgaactg tacaaatgac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     8161 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     8221 ccttcgggtg ggcctttctg cgtttatagc tgtaatacga ctcactatag gggataagag
     8281 acggctcaac gcccgtctca cagccgacag tcaatttgtc attatgaaaa tacacaaaag
     8341 ctttttccta tcttgcaaag cgacagctaa tttgtcacaa tcacggacaa cgacatctat
     8401 tttgtcactg caaagaggtt atgctaaaac tgccaaagcg ctataatcta tactgtataa
     8461 ggattttact gatgacaata atttgtcaca acgacatata attagtcact gtacaaggag
     8521 gactttggca ggtgcctgca ggattcttgc ttatcggcca gcctcgcaga gcaggattcc
     8581 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg
     8641 ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac
     8701 gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg
     8761 ctataatgac cccgaagcag ggttatgcag cggaaaagaa tgtcatgata ataatggttt
     8821 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8881 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8941 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9001 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9061 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     9121 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     9181 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     9241 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     9301 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     9361 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     9421 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9481 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9541 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9601 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9661 gagccggtga gcgtggctct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9721 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9781 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac ccggccacga
     9841 tgcgtccggc gtagaggatc tgaagatcag cagttcaacc tgttgatagt acgtactaag
     9901 ctctcatgtt tcacgtacta agctctcatg tttaacgtac taagctctca tgtttaacga
     9961 actaaaccct catggctaac gtactaagct ctcatggcta acgtactaag ctctcatgtt
    10021 tcacgtacta agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag
    10081 cctctaaggt tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagctttta
    10141 aggtttaacg gttgtgg
//
