LOCUS       sequence-371384-       10158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ShDART under control of Lac promoter, original configuration 
            (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, 
            GmR+sfGFP cargo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Rubin BE, Diamond S, Cress BF, Crits-Christoph A, Lou YC, Borges AL,
            Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R, Smock DCJ, Tang
            K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM,
            Banfield JF, Doudna JA
  TITLE     Species- and site-specific genome editing in complex bacterial 
            communities.
  JOURNAL   Nat Microbiol. 2022 Jan;7(1):34-47. doi: 10.1038/s41564-021-01014-7.
            Epub 2021 Dec 6.
  PUBMED    34873292
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-021-01014-7"; journalName: "Nat Microbiol"; date: 
            "2022-01"; volume: "7"; issue: "1"; pages: "34-47"
COMMENT     Sequence Label: pBFC0687
FEATURES             Location/Qualifiers
     source          1..10158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(174..179,180..197,198..204)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             205..216
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      5634..5705
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        5719..5753
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
     terminator      6008..6035
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        join(6213..6218,6219..6235,6236..6241)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             6430..6963
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     RBS             7322..7333
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             7340..8053
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      8065..8136
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8152..8179
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     8190..8209
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8190..8208
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     oriT            8492..8600
                     /note="incP origin of transfer"
     promoter        8793..8897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8898..8966,8967..9758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9116..9135)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9794..10158,1..24)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 cacaggaaca cttaacggct gacatgggca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgacacctg cccgaaccat atctttacac
      181 tttatgcttc cggctcgtat gttgaaagag gagaaaggat caatgaacag tcagcaaaat
      241 cctgatttag ctgttcatcc cttggcaatt cctatggaag gcttactagg agaaagtgct
      301 acaactcttg agaagaatgt aattgccaca caactctcag aggaagccca agtaaagcta
      361 gaggtaatcc aaagtttact ggaaccctgc gatcgcacaa cttatgggca aaagttgcgg
      421 gaagcagcag agaaactaaa tgtatcgttg cgaacggtac aaaggttggt gaaaaactgg
      481 gaacaagatg gcttagtcgg actcactcaa acaagtaggg ctgataaagg aaaacaccgc
      541 attggtgagt tttgggaaaa cttcattacc aaaacctaca aggagggtaa caagggaagt
      601 aaacgtatga cccctaaaca agttgctctc agagtcgagg ctaaagcccg tgaattaaaa
      661 gactctaagc cgcccaatta caaaaccgtg ttacgggtat tagcacccat tttggaaaag
      721 caacaaaaag ccaagagtat ccgcagtcct ggttggagag gaactacgct ttcggttaaa
      781 acccgtgaag gaaaagattt atcggttgat tacagtaacc atgtttggca atgtgaccat
      841 acccgcgtgg atgtgttgct ggtagatcaa catggtgaaa ttttaagtcg tccctggcta
      901 acaacagtaa ttgatactta ctctcgttgc attatgggta tcaacttggg ctttgatgca
      961 cccagttctg gggtagtagc attagcgtta cgccatgcaa ttctaccaaa gcgttacggt
     1021 tccgagtaca aactgcattg tgagtgggga acctatggaa aaccagaaca tttttatact
     1081 gatggcggta aagactttcg ctctaaccac ttgagtcaga ttggggcgca attgggattt
     1141 gtctgtcatt tacgcgatcg cccttctgaa ggtggagtag tagaacgtcc cttcaaaaca
     1201 ttaaatgacc aactattttc aacgcttcct gggtacaccg gatctaatgt gcaggaacgc
     1261 ccagaagatg cagagaagga cgcaagactt actttgcgag aactagaaca gttacttgtg
     1321 cgttacatcg tagatcgtta caaccaaagt attgatgcgc ggatgggcga ccaaacgcgc
     1381 tttgagcgtt gggaagcagg attgcctaca gtgccagtac caataccaga acgagatttg
     1441 gatatttgtt taatgaagca gtcacggcgc actgtgcaaa gaggtggttg tttgcagttt
     1501 cagaatttaa tgtatcgggg ggaatatttg gcaggttatg ccggagaaac tgtcaactta
     1561 aggtttgacc ccagagacat tacaacaatt ttggtttatc gccaggaaaa caatcaggaa
     1621 gtatttctga ctcgcgctca cgctcaaggt ttggagacag agcaactggc attagatgag
     1681 gctgaggcag caagtcgcag actccgtacc gcagggaaaa ctatcagtaa ccaatcatta
     1741 ttgcaagaag ttgttgaccg cgatgctctt gtcgctacca agaaaagccg taaggagcgt
     1801 caaaaattgg aacagactgt tttgcgatct gctgctgttg atgaaagtaa tagagaatcc
     1861 ttgccttctc aaatagttga accagatgaa gtggaatcta cagaaacggt tcactctcaa
     1921 tacgaagata ttgaggtgtg ggactatgaa caacttcgtg aagaatatgg gttttaaaca
     1981 atgacagaag ctcaggcgat cgccaagcag ttgggtgggg taaaaccgga tgatgagtgg
     2041 ttacaagctg aaattgctcg tcttaagggt aagagcattg tgcctttaca gcaggtaaaa
     2101 actctccatg attggttaga tggcaagcgc aaggcaagaa aatcttgccg agtagttggg
     2161 gaatcgagaa ctggcaagac agttgcttgt gatgcctaca gatacaggca caaacctcag
     2221 caggaagctg gacgacctcc aactgtgcct gtcgtttata ttcgacctca ccaaaaatgt
     2281 ggccccaagg atttgtttaa aaagattact gagtacctca agtatcgggt aacaaaaggg
     2341 actgtatctg attttcgaga taggacgata gaagtactca agggttgtgg cgtagagatg
     2401 ctaattattg atgaagctga ccgtcttaag cctgaaactt ttgctgatgt gcgagatatt
     2461 gccgaagatt taggaattgc tgtggtactg gtaggaacag accgtttgga tgcggtaatt
     2521 aagcgggatg agcaggttct cgaacgcttt cgggcgcatc ttcgctttgg taaattgtcg
     2581 ggagaggatt ttaagaacac cgtagaaatg tgggaacaaa tggttttgaa actgccagta
     2641 tcttctaatc taaagagcaa ggagatgcta cggattctca cgtcagcaac tgaaggctac
     2701 attggtcgcc ttgatgagat tcttagggaa gctgcaattc gttccttatc aagaggattg
     2761 aagaagattg acaaggctgt tttacaggaa gtagctaagg agtacaaatg atagaagcac
     2821 cagatgttaa accttggcta ttcttgatta aaccctatga aggggaaagc ctgagccact
     2881 ttcttggcag gttcagacgt gccaaccatt tatccgcaag tggattgggt actttggcag
     2941 gaattggtgc tatagtggca cgttgggaaa gatttcattt taatcctcgc cctagtcagc
     3001 aagaattgga agcgatcgca tctgtagtag aagtggatgc tcaaaggtta gcccagatgt
     3061 taccgcctgc tggagtggga atgcagcatg agccaattcg cttgtgtggg gcttgttatg
     3121 ccgagtcgcc ttgtcaccga attgaatggc agtacaagtc ggtgtggaag tgcgatcgcc
     3181 atcaactcaa gattttagca aagtgtccaa actgtcaagc accttttaaa atgcctgcgc
     3241 tgtgggagga tgggtgctgt cacagatgta ggatgccgtt tgcagaaatg gcaaagctac
     3301 agaaggtttg atgataaaac cagaaaaagg tgtgaaatta actaagtccc tgaattgatc
     3361 tggttgtcca aaaaatttgt gcgatcgcat ggcaagatta ttcctactat tgatgtggcg
     3421 tgagtcggat aacttgctct aatgggatcc cggttaatac caatagatca gatatgagta
     3481 ataaacatta cgacctttac ctgtggtatt cactgttggc acgacaacac ctttatcgcg
     3541 ccaatactgt agctgacgac gcgaacaatt agtgatttga gccgcttctg tgcttgtgta
     3601 gaattttcct tccatagaaa tcatcctaca gaagtagttt tttaaattta cattttttaa
     3661 aagcacatta tgtattcatg tgatattatt tttttatgag tcaaataact attcaagctc
     3721 gacttatttc ctttgaatca aaccgccaac aactctggaa gttgatggca gatttaaaca
     3781 cgccgttaat taacgaactg ctttgccagt taggtcaaca ccccgacttc gagaagtggc
     3841 aacaaaaggg taaactcccg tctaccgttg tgagccagtt atgtcaacct ctcaaaactg
     3901 accctcgctt tgcaggtcag cccagccgtt tatatatgtc ggcaattcat attgtggact
     3961 acatctacaa gtcctggctg gctatacaga aacggcttca acagcagcta gatggaaaga
     4021 cgcgctggct agaaatgctc aatagcgatg ctgaattagt agaacttagt ggtgacactt
     4081 tagaggctat tcgtgtcaaa gctgctgaaa ttttggcaat agctatgcca gcatctgagt
     4141 cagatagcgc ttcacctaaa gggaaaaaag gtaaaaagga gaaaaaaccc tcatcttcta
     4201 gccctaagcg tagtttatcc aagacattat ttgacgctta ccaagaaacg gaagatatca
     4261 agagccgtag cgccatcagc tacctgttaa aaaatggctg caaacttact gacaaagaag
     4321 aagattcaga aaaatttgct aaacgtcgtc gtcaagttga aatccaaatt caaaggctta
     4381 ccgaaaagtt aataagtcgg atgcctaaag gtcgagattt gaccaatgct aaatggttgg
     4441 agacactctt gactgctaca accactgttg ctgaagataa cgcccaagcc aaacgctggc
     4501 aggatattct gttaactcga tcaagttctc tcccattccc ccttgttttt gaaaccaacg
     4561 aggatatggt ttggtcaaag aatcaaaagg gtaggctgtg tgttcacttc aatggcttaa
     4621 gcgatttaat ttttgaggtg tactgcggca atcgtcaact tcactggttt caacgcttcc
     4681 tagaagatca acagactaaa cgcaaaagca aaaatcagca ttctagcggc ttgttcacac
     4741 tcagaaatgg tcatctagtt tggcttgaag gtgagggtaa aggggaacct tggaatcttc
     4801 accacttgac cctttactgc tgtgttgaca atcgcttgtg gacagaggag ggaacagaaa
     4861 tcgttcgcca agagaaagca gatgaaatta ctaaattcat cacaaacatg aagaagaaaa
     4921 gcgatctaag cgatacacag caagctttga ttcaacgtaa acaatcaaca cttactcgaa
     4981 taaacaattc ctttgagcgt cctagccaac ccctttatca aggtcaatca cacattttgg
     5041 ttggagtaag cctgggacta gaaaaacctg ccacagtagc agtagtagat gcgatcgcca
     5101 acaaagtctt ggcttaccgg agtattaaac aattacttgg cgacaattac gaactgctaa
     5161 atcgccaaag acgacaacag cagtacctat ctcacgaacg ccacaaagca caaaaaaact
     5221 tctctcccaa tcaatttgga gcatctgagt tagggcaaca tatagacaga ttattagcta
     5281 aagcaattgt agcgttagcg agaacctaca aagctggcag tattgtcttg cccaagttag
     5341 gggatatgcg ggaggttgtc caaagtgaaa ttcaagctat agcagaacaa aaatttcccg
     5401 gttatattga aggtcagcaa aaatatgcca aacagtaccg ggttaatgtt catcggtgga
     5461 gctacggcag attaattcaa agcattcaaa gtaaagcagc tcaaacagga attgtgattg
     5521 aggagggaaa acaacctatt cgaggtagtc cccacgacaa agcaaaggaa ttagcacttt
     5581 ctgcttacaa tctccgccta actaggcgaa gttaagtaag gatctccagg catcaaataa
     5641 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     5701 ctctctacta gacagaccct gacagctagc tcagtcctag gtataatgct agcatattaa
     5761 tagcgccgca attcatgctg cttgcagcct ctgaattttg ttaaatgagg gttagtttga
     5821 ctgtataaat acagtcttgc tttctgaccc tggtagctgc tcaccctgat gctgctgtca
     5881 atagacagga taggtgcgct cccagcaata agggcgcgga tgtactgctg tagtggctac
     5941 tgaatcaccc ccgatcaagg gggaacccta aatgggttga aagggagacc gagatctcga
     6001 ggtctccggc tcaccttcgg gtgggccttt ctgcgcaggt ccaaaccaga tgtcaacaca
     6061 gctactgtac agtgacaaat tatctgtcgt cggtgacaga ttaatgtcat tgtgactatt
     6121 taattgtcgt cgtgacccat cagcgttgct taattaattg atgacaaatt aaatgtcatc
     6181 agccaggggt ccccaataat tacgatttaa atttgacata agcctgttcg gttcgtaaac
     6241 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     6301 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt gtacagccta
     6361 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     6421 gcagcaacga tgttacgcag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     6481 aagttaggtg gctcaagtat gggcatcatt cgcacgtgta ggctcggccc tgaccaagtc
     6541 aaatccatgc gggctgctct tgatcttttc ggtcgtgagt tcggtgacgt agccacctac
     6601 tcccaacatc agccggattc cgattacctc gggaacttgc tccgtagtaa gacattcatc
     6661 gcgcttgctg ccttcgacca agaagcggtt gttggcgctc tcgcggctta cgttctgccc
     6721 aagtttgagc agccgcgtag tgaaatctat atctatgatc tcgcagtctc cggagagcac
     6781 cggaggcagg gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt
     6841 ggtgcttatg tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat
     6901 acaaagttgg gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc
     6961 taacaattcg ttcaagccga gatcggcttc ccggccgcgg agttgttcgg taaattggac
     7021 aacggtccgc gcgttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat
     7081 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt
     7141 agactttcct tggtgtatcc aacggcgtca gccgaaagtg aaacgtgatt tcatgcgtca
     7201 ttttgaacat tttgtaaatc ttatttaata atgtgtgcgg caattcacat ttaatttatg
     7261 aatgttttct taacatcgcg gcaactcaag aaacggcagg ttcggatctt agctactaga
     7321 gaaagaggag aaatactaga tgcgtaaagg cgaggagctg ttcactggtg tcgtccctat
     7381 tctggtggaa ctggatggtg atgtcaacgg tcataagttt tccgtgcgtg gcgagggtga
     7441 aggtgacgca actaatggta aactgacgct gaagttcatc tgtactactg gtaaactgcc
     7501 ggttccttgg ccgactctgg taacgacgct gacttatggt gttcagtgct ttgctcgtta
     7561 tccggaccat atgaagcagc atgacttctt caagtccgcc atgccggaag gctatgtgca
     7621 ggaacgcacg atttccttta aggatgacgg cacgtacaaa acgcgtgcgg aagtgaaatt
     7681 tgaaggcgat accctggtaa accgcattga gctgaaaggc attgacttta aagaggacgg
     7741 caatatcctg ggccataagc tggaatacaa ttttaacagc cacaatgttt acatcaccgc
     7801 cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt cgccacaacg tggaggatgg
     7861 cagcgtgcag ctggctgatc actaccagca aaacactcca atcggtgatg gtcctgttct
     7921 gctgccagac aatcactatc tgagcacgca aagcgttctg tctaaagatc cgaacgagaa
     7981 acgcgatcat atggttctgc tggagttcgt aaccgcagcg ggcatcacgc atggtatgga
     8041 tgaactgtac aaatgaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     8101 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     8161 tcgggtgggc ctttctgcgt ttatagctgt aatacgactc actatagggg ataagagacg
     8221 gctcaacgcc cgtctcacag ccgacagtca atttgtcatt atgaaaatac acaaaagctt
     8281 tttcctatct tgcaaagcga cagctaattt gtcacaatca cggacaacga catctatttt
     8341 gtcactgcaa agaggttatg ctaaaactgc caaagcgcta taatctatac tgtataagga
     8401 ttttactgat gacaataatt tgtcacaacg acatataatt agtcactgta caaggaggac
     8461 tttggcaggt gcctgcagga ttcttgctta tcggccagcc tcgcagagca ggattcccgt
     8521 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc
     8581 ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa
     8641 ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta
     8701 taatgacccc gaagcagggt tatgcagcgg aaaagaatgt catgataata atggtttctt
     8761 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8821 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8881 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8941 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9001 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9061 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9121 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9181 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9241 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9301 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9361 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9421 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9481 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9541 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9601 ccggtgagcg tggctctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9661 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9721 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagacccg gccacgatgc
     9781 gtccggcgta gaggatctga agatcagcag ttcaacctgt tgatagtacg tactaagctc
     9841 tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt ttaacgaact
     9901 aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc tcatgtttca
     9961 cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct
    10021 ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg
    10081 tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct tagagcctct
    10141 caaagcaatt ttgagtga
//
