LOCUS       sequence-371385-       12650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP 
            cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right
            End.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12650)
  AUTHORS   Rubin BE, Diamond S, Cress BF, Crits-Christoph A, Lou YC, Borges AL,
            Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R, Smock DCJ, Tang
            K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM,
            Banfield JF, Doudna JA
  TITLE     Species- and site-specific genome editing in complex bacterial 
            communities.
  JOURNAL   Nat Microbiol. 2022 Jan;7(1):34-47. doi: 10.1038/s41564-021-01014-7.
            Epub 2021 Dec 6.
  PUBMED    34873292
REFERENCE   2  (bases 1 to 12650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-021-01014-7"; journalName: "Nat Microbiol"; date: 
            "2022-01"; volume: "7"; issue: "1"; pages: "34-47"
COMMENT     Sequence Label: pBFC1207
FEATURES             Location/Qualifiers
     source          1..12650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(173..178,179..196,197..203)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             204..215
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2300..2311
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(8800..8805,8806..8822,8823..8828)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             9017..9550
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     RBS             9909..9920
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             9927..10640
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      10652..10723
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10739..10766
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     10777..10796
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10777..10795
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     10919..10939
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     oriT            10983..11091
                     /note="incP origin of transfer"
     promoter        11284..11388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11389..11457,11458..12249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11607..11626)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(12285..12650,1..23)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 acaggaacac ttaacggctg acatgggcaa gtttactcat atatacttta gattgattta
       61 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      121 aaaatccctt aacgtgagtt ttcgttccac tgacacctgc ccgaaccata tctttacact
      181 ttatgcttcc ggctcgtatg ttgaaagagg agaaaggatc aatggcgaca agtttaccta
      241 cgccctcagc aattacgact tcggcgttag agtatgcatt ccatactccc gctcgcaatc
      301 taacgaaatc tcgcggaaaa aacattcatc gttatgtcag tgtaaagatg agtaagagga
      361 ttacggtaga atctactcta gagtgtgatg cctgctatca ctttgatttt gagccaagta
      421 ttgttcgctt ttgcgctcaa ccgattcgat ttttatatta tctcaatggt cagtctcact
      481 cctatgttcc tgactttcta gttcaatttg ataccaacga gtttgttcta tatgaagtaa
      541 agtcagctta tgctaagaac aaacctgatt ttgatgttga atgggaggcg aaagtaaaag
      601 cagcaactga actagggcta gaattggagc ttgttgaaga gagtgatatt agggatacgg
      661 ttgtattaaa taatcttaag cgcatgcatc gttatgcttc gaaagatgag ctgaataacg
      721 tacataactc tctcttaaaa ataataaagt acaatggcgc ccaatctgca agatgcttgg
      781 gagaacagtt gggtttaaaa ggccgaactg ttttaccaat tttgtgcgat ttgctgtcaa
      841 ggtgtttact cgatacacgt ttggataagc ctctatctct tgaatctcga tttgagttgg
      901 ccagttatgg ctaagaaagg gttctcaagt ttccatagaa aagcagtctc ttcccaagat
      961 acgcttgaat cgatagagct tgtctctagc gctaattgtc tagaaagtgt tacgtatcaa
     1021 gatatatcag catttcccga aacaattgcg gtagagatta atttccgatt aagcattctt
     1081 cgctttttag cgcgaaagtg cgaaaccatt gtggccaaat caattgaacc acatcgtgta
     1141 gagctacagc aaaactatag tagaaaaata cccagtgcaa taacgatata tcgatggtgg
     1201 cttgcttttc gaaaatcaga ctacaacccc attagcttag cacctaatat caaggataga
     1261 ggtaatagag agacaaaagt gtcaacagtt gttgattcta ttatggaaca ggcagttgaa
     1321 agagttatat ctggacgaaa agtcaatgtt agctctgcat ataaacgtgt tcgacgaaaa
     1381 gttcgtcaat acaatctaac tcatggaacg aaatacacgt atcctaagta cgaatctgta
     1441 agaaagcgag taaaaaagaa aaccccattt gagttattag ccgcagggaa aggggagaga
     1501 gtagctaaga gagagtttcg ccgaatggga aaaaagatcc tcacgtctag cgtgctagag
     1561 agggttgaaa tagatcacac tgtcgttgac ctttttgcag tacacgaaga gtatcgaatc
     1621 ccattgggcc gaccttggct tactcaattg gttgattgtt acagtaaagc tgtaatcggt
     1681 ttttatttag gtttcgagcc tcctagctat gtgtcggttt cccttgcact taagaatgca
     1741 atacaacgca aagatgactt aatctcctcg tatgaatcga tcgagaatga atggctatgt
     1801 tatggcatcc cagacctact cgtaactgat aatggtaaag agtttttgtc gaaagcattt
     1861 gatcaagcat gtgaatcact attgatcaat gtgcatcaaa ataaagttga aacgcccgac
     1921 aacaaacctc atgttgaacg taactacggg actattaata cttctctgtt agacgattta
     1981 cctgggaaat ccttcagcca gtaccttcaa agagaagggt acgactctgt gggagaagct
     2041 acccttacac tcaatgagat tagagaaatt tacttaattt ggttggtgga tatttatcat
     2101 aaaaaaccca atcagagagg cactaattgt cctaatgttg cgtggaaaaa gggttgtcaa
     2161 gaatgggaac cagaggagtt ctctggttct aaagacgaat tagactttaa atttgctatt
     2221 gttgattaca aacaacttac taaagtaggg ataactgtct acaaagaact gagttatagc
     2281 aatgaccgtt tagctgaata tagagggaag aaaggaaacc ataaagttca gttcaagtat
     2341 aaccctgagt gtatggcagt tatttgggtg ttggatgagg atatgaatga gtactttaca
     2401 gttaatgcga ttgactacga atatgcaagt agagtatcac tttggcaaca taaatataac
     2461 atgaaatatc aagcagaact aaattcagca gaatatgatg aggacaagga aattgatgca
     2521 gaaataaaaa ttgaagaaat cgcagatcgt tcaattgtta agactaacaa aatcagagct
     2581 cggaggcgtg gcgctaggca tcaagagaat agcgcaaggg ctaagtcaat cagtaatgcg
     2641 aacccggcct cgatacaaaa acatgaagat gaaatcgtta gtgcagataa tgacgattgg
     2701 gatattgatt atgtctgaga aatcgtcaaa tgagtgaaac gcgtgaggct cgtatatcaa
     2761 gagctaaaag ggcatttgta tccacaccga gcgttaggaa aatcttgagt tacatggata
     2821 gatgtagaga tctatcagac ctagagtctg agcctacatg catgatggtc tatggtgctt
     2881 cgggtgtagg taaaacgacc gtcatcaaga aatacttaaa tcaaaacaga agagagtccg
     2941 aagccggggg cgatataata ccggttttgc atattgagtt gccagacaat gcgaagccag
     3001 tagatgcagc aagggaattg ctggttgaaa tgggtgaccc gctagcactt tatgaaactg
     3061 acttagctag attgacgaaa agactgactg aattaatccc tgcggtcggc gtgaagctga
     3121 ttattatcga tgagttccaa catttggtgg aagaaaggtc aaatcgggtt cttacccaag
     3181 taggtaattg gctaaaaatg atacttaaca aaacgaaatg tccaattgtt atatttggta
     3241 tgccatactc aaaagttgta ctgcaagcaa actcgcaact tcacgggcga ttttccattc
     3301 aggttgaact gcgccccttt agctaccagg gaggtagagg tgtatttaaa acttttttgg
     3361 aataccttga taaagcccta ccttttgaaa aacaggctgg cttagccaac gaaagtttgc
     3421 agaaaaaatt gtatgcattc tctcagggaa acatgcgttc gttgagaaac cttatttatc
     3481 aagcatctat cgaagcaatt gataatcagc atgaaacgat aaccgaagaa gatttcgttt
     3541 ttgcatcgaa gttgacatcg ggcgataaac ccaactcatg gaaaaatcct tttgaggagg
     3601 gtgttgaggt aacagaagat atgttacgac cgccaccaaa agatattggt tgggaggact
     3661 atttgagaca ttcaaccccg agagtgagta aaccaggtag aaataaaaac tttttcgaat
     3721 aacctaggct gctgccaccg ctgagcaata accatcttag tatattagtt aagtataaga
     3781 aggagatata catatgtttt tgcaaagacc taaaccttac agcgatgaaa gtttagaaag
     3841 tttctttatc cgagtggcta acaaaaatgg ctacggtgat gtccatcgct tcctagaagc
     3901 cactaaacga ttccttcaag acattgacca taatggctat caaacctttc cgactgatat
     3961 aactcggata aacccatact cagctaaaaa cagttccagc gcacgaactg cgtcattcct
     4021 gaaacttgca caattgacat ttaatgaacc gccagagcta cttgggttgg caattaacag
     4081 aacaaacatg aaatactcgc cgtcaactag cgcggttgtt agaggtgcag aagtctttcc
     4141 tcgcagttta ctacggacgc actccatccc ctgctgtcct ttgtgtctgc gagaaaatgg
     4201 ctacgcctcc tacctttggc actttcaggg gtacgaatac tgccacagcc ataacgtacc
     4261 tttaattacc acttgtagct gtggtaagga gtttgactac cgagtatctg ggttaaaggg
     4321 catttgctgc aaatgcaagg agcctatcac cttaaccagc agggagaacg gtcatgaggc
     4381 agcgtgtact gtttcaaact ggcttgctgg ccatgaatct aaacctctgc caaatcttcc
     4441 taaaagctac cgatggggtt tagttcattg gtggatgggt attaaagata gcgagttcga
     4501 tcacttttcg ttcgttcaat ttttctcaaa ctggccaagg tcattccact cgataatcga
     4561 agatgaagta gagttcaacc ttgagcatgc tgttgtcagc acgtctgaat tacgactaaa
     4621 agatcttctt ggtcgattgt ttttcggttc aattcggtta cctgagcgga atcttcaaca
     4681 caatatcatc cttggtgagc ttctctgcta tttagaaaat cgtttatggc aagacaaggg
     4741 attaatcgcc aacctcaaaa tgaacgcgtt agaggcgact gtaatgttaa attgtagcct
     4801 cgatcagatt gcatcaatgg ttgaacaacg catcttgaag ccaaatcgaa aaagcaagcc
     4861 caacagccct cttgatgtta ccgattatct atttcatttc ggcgatattt tctgtctttg
     4921 gttagctgag ttccaaagcg atgagtttaa ccgttcgttt tatgtgtcga ggtggtaaat
     4981 gcaaactctg aaagaactaa tcgcatccaa tcctgacgac ttaacaactg aacttaagag
     5041 agcatttcgt ccactcacac cgcatattgc aattgatggt aatgaacttg acgcactgac
     5101 gatattagtc aatttaaccg ataagactga tgatcagaaa gacctgctcg atcgagccaa
     5161 atgcaagcaa aaacttcgag atgaaaaatg gtgggctagc tgcataaatt gcgttaatta
     5221 cagacaaagc cataacccaa aattcccgga tatacgttct gaaggcgtga ttcgaaccca
     5281 agccctgggt gaattaccga gtttcctact ctcgtcctca aaaatcccac cataccattg
     5341 gtcatatagc catgattcaa aatacgtcaa caaaagcgca ttcctcacca atgagttttg
     5401 ttgggatggt gagatctcat gtttgggtga gcttcttaaa gatgcagatc acccactttg
     5461 gaatactttg aaaaagttag gttgttctca aaaaacttgc aaagcaatgg caaaacaact
     5521 agctgatatt actctcacga ctatcaatgt cacactcgca ccaaattacc tgactcaaat
     5581 atctctcccc gatagtgata catcctacat ttcactctct cctgttgcat cgctatcgat
     5641 gcaaagccac tttcatcaga ggctccaaga tgaaaatcgg catagcgcga taacgcggtt
     5701 tagccgaact accaacatgg gggtcactgc gatgacatgt ggcggcgcat ttagaatgtt
     5761 aaagtctggc gctaagtttt ccagcccccc tcatcaccga ttaaacagta aacgaagttg
     5821 gttgacgtca gagcatgttc agtcattaaa acagtaccag cgcctcaaca aaagcctcat
     5881 acctgaaaac tctcggattg cactccgtag aaaatacaaa atcgagcttc aaaatatggt
     5941 cagatcttgg tttgcaatgc aagaccatac ccttgattcg aacatactta tccaacattt
     6001 gaatcatgac ctatcttact taggagccac aaaacgtttt gcatacgatc cagcgatgac
     6061 caagctcttt actgagcttt tgaaacgaga gttatcaaat tcaatcaata atggtgagca
     6121 acacactaat ggatcgtttt tagtcctacc gaatatcagg gtttgtggcg caacagcttt
     6181 aagctccccg gtaacggtgg ggattccatc acttacagct ttctttggct tcgttcacgc
     6241 atttgaacgg aatataaatc gcaccacctc atcgtttcgt gttgaatcct ttgcgatatg
     6301 cgtccatcaa ctacatgtcg agaagcgagg tttgacagca gagtttgtgg aaaaaggcga
     6361 cgggactata tccgctcccg cgacccggga tgactggcag tgtgatgtcg tatttagcct
     6421 tattttgaac accaactttg ctcaacatat tgaccaagat acgttagtta catcactacc
     6481 aaagcgattg gctcggggtt cagcaaaaat tgcgattgat gactttaaac atatcaactc
     6541 attctcgaca ttagaaacag cgatcgaatc tctgccaata gaagctggta ggtggttatc
     6601 actttacgca cagtcaaaca ataatctaag tgatctatta gcagccatga cagaggacca
     6661 tcagctcatg gcaagctgcg tcggttacca cttgttagaa gaacccaaag ataaaccaaa
     6721 ctccctcaga ggttacaaac acgctatcgc cgagtgcatc attggactca ttaactcaat
     6781 cacctttagc tcagagactg atcccaacac aatcttttgg tcgctaaaga actatcaaaa
     6841 ctacctagtg gtacagccaa ggagtatcaa cgatgaaact accgacaaat ctagcctatg
     6901 agcgctctat cgacccatca gatgtctgtt tttttgtcgt ctggcccgat gatagaaaaa
     6961 cacctttaac ctacaattct cgtactctgc tcgggcaaat ggaagcggca tcattagcct
     7021 atgatgtctc aggtcaacca ataaaaagtg ccaccgctga ggcgttagct caagggaacc
     7081 ctcatcaagt tgatttctgc cacgttccat acggtgcgag tcatattgaa tgcagtttct
     7141 ccgtttcgtt ttcttctgaa ctacgtcaac catataagtg taactcaagc aaagttaaac
     7201 aaacgctagt gcaattagtc gagctctacg aaacgaaaat cggctggact gagctagcaa
     7261 cccgatattt gatgaacatt tgcaacggta aatggctgtg gaaaaatacc cgtaaagcct
     7321 attgctggaa cattgtactt acaccttggc cttggaacgg ggaaaaggtt ggatttgaag
     7381 atattcgtac taactacacc tcacggcaag actttaaaaa taataaaaat tggtctgcta
     7441 tagttgaaat gatcaaaacc gcattttcta gtactgatgg gctggcgata tttgaagtca
     7501 gggccacctt gcacttgcca acgaatgcta tggtgcggcc aagccaagtt ttcacagaaa
     7561 aagaaagtgg cagtaaaagt aaatctaaaa ctcaaaacag tcgagttttt cagagtacaa
     7621 ctattgatgg tgaacgatcg ccaatactag gggcctttaa aacgggagca gctattgcaa
     7681 ccattgacga ctggtatcct gaagccactg agccactaag ggtcggacgg tttggggttc
     7741 atcgcgaaga tgtcacttgc taccgtcatc cgtctaccgg aaaagatttt ttctcgatat
     7801 tacaacaagc agagcactat attgaagtgt tgagcgccaa caaaactccc gctcaagaaa
     7861 ctatcaacga catgcacttt ttaatggcta acctgattaa gggtgggatg ttccagcata
     7921 aaggagactg actgtgaaat ggtattataa gacaatcacc tttctgccag agttgtgcaa
     7981 caacgagtca ctggctgcaa agtgtctccg cgttctgcat ggatttaact atcagtatga
     8041 gacacgaaat atcggcgttt catttccgct ttggtgtgat gcaacggttg gaaaaaagat
     8101 ttcatttgtc agcaagaaca agatagaact cgacttacta cttaaacaac actatttcgt
     8161 ccaaatggaa caacttcaat actttcatat atccaacact gttctcgtcc cagaagattg
     8221 tacatacgtt tcctttagac gctgtcaatc tatagataag ctcacagcag cagggctggc
     8281 aaggaaaatc agacgcctgg agaaacgtgc tctatctaga ggcgagcaat ttgacccatc
     8341 atcttttgct caaaaagagc atactgcaat agcgcactac cactcacttg gggagtccag
     8401 caaacagacg aaccgcaact ttcgactcaa tatcaggatg ctctcggagc aaccccgtga
     8461 ggggaactcg atttttagta gctatggctt atcaaattca gaaaactcgt ttcagcctgt
     8521 acccttaatc tgacctaggc tgctgccacc ggtgaactgc cgagtaggta gctgataacg
     8581 agacctcgtt tacctatcgg tctcgtgaac tgccgagtag gtagctgata acccaaacca
     8641 gatgtcaaca cagctactgt tgatgcaacc ataaagtgat atttaataat tatttataat
     8701 cagcaactta accacaaaac aaccatatat tgatatctca caaaacaacc ataagttgat
     8761 atttttcagc caggggtccc caataattac gatttaaatt tgacataagc ctgttcggtt
     8821 cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     8881 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta
     8941 cagcctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     9001 ttatggagca gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc
     9061 taaaacaaag ttaggtggct caagtatggg catcattcgc acgtgtaggc tcggccctga
     9121 ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gtgacgtagc
     9181 cacctactcc caacatcagc cggattccga ttacctcggg aacttgctcc gtagtaagac
     9241 attcatcgcg cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt
     9301 tctgcccaag tttgagcagc cgcgtagtga aatctatatc tatgatctcg cagtctccgg
     9361 agagcaccgg aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa
     9421 cgcgcttggt gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc
     9481 tctctataca aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac
     9541 cgccacctaa caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa
     9601 attggacaac ggtccgcgcg ttgtcctttt ccgctgcata accctgcttc ggggtcatta
     9661 tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg
     9721 ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gaaagtgaaa cgtgatttca
     9781 tgcgtcattt tgaacatttt gtaaatctta tttaataatg tgtgcggcaa ttcacattta
     9841 atttatgaat gttttcttaa catcgcggca actcaagaaa cggcaggttc ggatcttagc
     9901 tactagagaa agaggagaaa tactagatgc gtaaaggcga ggagctgttc actggtgtcg
     9961 tccctattct ggtggaactg gatggtgatg tcaacggtca taagttttcc gtgcgtggcg
    10021 agggtgaagg tgacgcaact aatggtaaac tgacgctgaa gttcatctgt actactggta
    10081 aactgccggt tccttggccg actctggtaa cgacgctgac ttatggtgtt cagtgctttg
    10141 ctcgttatcc ggaccatatg aagcagcatg acttcttcaa gtccgccatg ccggaaggct
    10201 atgtgcagga acgcacgatt tcctttaagg atgacggcac gtacaaaacg cgtgcggaag
    10261 tgaaatttga aggcgatacc ctggtaaacc gcattgagct gaaaggcatt gactttaaag
    10321 aggacggcaa tatcctgggc cataagctgg aatacaattt taacagccac aatgtttaca
    10381 tcaccgccga taaacaaaaa aatggcatta aagcgaattt taaaattcgc cacaacgtgg
    10441 aggatggcag cgtgcagctg gctgatcact accagcaaaa cactccaatc ggtgatggtc
    10501 ctgttctgct gccagacaat cactatctga gcacgcaaag cgttctgtct aaagatccga
    10561 acgagaaacg cgatcatatg gttctgctgg agttcgtaac cgcagcgggc atcacgcatg
    10621 gtatggatga actgtacaaa tgaccaggca tcaaataaaa cgaaaggctc agtcgaaaga
    10681 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
    10741 ctcaccttcg ggtgggcctt tctgcgttta tagctgtaat acgactcact ataggggata
    10801 agagacggct caacgcccgt ctcacagcgc aatatcaatt tatgggtgtg ataattatca
    10861 atttatgggt gtaattatca ttttatggtt gtatcaacaa ggaggacttt ggcaggtgca
    10921 gcacaaaagg aaactcaccg cgatcgccat tactcgcatc agcctgcagg attcttgctt
    10981 atcggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
    11041 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
    11101 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
    11161 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
    11221 gaaaagaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
    11281 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    11341 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    11401 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    11461 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    11521 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    11581 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
    11641 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    11701 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    11761 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    11821 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    11881 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
    11941 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    12001 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    12061 gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg cggtatcatt
    12121 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    12181 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    12241 cattggtaac tgtcagaccc ggccacgatg cgtccggcgt agaggatctg aagatcagca
    12301 gttcaacctg ttgatagtac gtactaagct ctcatgtttc acgtactaag ctctcatgtt
    12361 taacgtacta agctctcatg tttaacgaac taaaccctca tggctaacgt actaagctct
    12421 catggctaac gtactaagct ctcatgtttc acgtactaag ctctcatgtt tgaacaataa
    12481 aattaatata aatcagcaac ttaaatagcc tctaaggttt taagttttat aagaaaaaaa
    12541 agaatatata aggcttttaa agcttttaag gtttaacggt tgtggacaac aagccaggga
    12601 tgtaacgcac tgagaagccc ttagagcctc tcaaagcaat tttgagtgac
//
