LOCUS       sequence-371386-       12613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  VcDART under control of Lac promoter, Vc_lacZ_alpha_1 gRNA, 
            GmR+sfGFP cargo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Rubin BE, Diamond S, Cress BF, Crits-Christoph A, Lou YC, Borges AL,
            Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R, Smock DCJ, Tang
            K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM,
            Banfield JF, Doudna JA
  TITLE     Species- and site-specific genome editing in complex bacterial 
            communities.
  JOURNAL   Nat Microbiol. 2022 Jan;7(1):34-47. doi: 10.1038/s41564-021-01014-7.
            Epub 2021 Dec 6.
  PUBMED    34873292
REFERENCE   2  (bases 1 to 12613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-021-01014-7"; journalName: "Nat Microbiol"; date: 
            "2022-01"; volume: "7"; issue: "1"; pages: "34-47"
COMMENT     Sequence Label: pBFC0625
FEATURES             Location/Qualifiers
     source          1..12613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      270..341
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      357..384
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     395..414
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        395..413
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     oriT            557..665
                     /note="incP origin of transfer"
     promoter        858..962
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(963..1031,1032..1823)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1181..1200)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1859..2247
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     promoter        join(2397..2402,2403..2420,2421..2427)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             2428..2439
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4524..4535
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(11031..11036,11037..11053,11054..11059)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             11248..11781
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     RBS             12140..12151
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(12158..12613,1..258)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 accgccgata aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag
       61 gatggcagcg tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct
      121 gttctgctgc cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac
      181 gagaaacgcg atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt
      241 atggatgaac tgtacaaatg accaggcatc aaataaaacg aaaggctcag tcgaaagact
      301 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      361 caccttcggg tgggcctttc tgcgtttata gctgtaatac gactcactat aggggataag
      421 agacggctca acgcccgtct cacagcgcaa tatcaattta tgggtgtgat aattatcaat
      481 ttatgggtgt aattatcatt ttatggttgt atcaacaagg aggactttgg caggtgcctg
      541 caggattctt gcttatcggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
      601 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
      661 tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc
      721 ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc
      781 agggttatgc agcggaaaag aatgtcatga taataatggt ttcttagacg tcaggtggca
      841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      961 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1021 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1081 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1141 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1201 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1261 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1321 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1381 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1441 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1501 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1561 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1621 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggct
     1681 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1741 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1801 cctcactgat taagcattgg taactgtcag acccggccac gatgcgtccg gcgtagagga
     1861 tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg tttcacgtac
     1921 taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc ctcatggcta
     1981 acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac taagctctca
     2041 tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag gttttaagtt
     2101 ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa cggttgtgga
     2161 caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag caattttgag
     2221 tgacacagga acacttaacg gctgacatgg gcaagtttac tcatatatac tttagattga
     2281 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2341 gaccaaaatc ccttaacgtg agttttcgtt ccactgacac ctgcccgaac catatcttta
     2401 cactttatgc ttccggctcg tatgttgaaa gaggagaaag gatcaatggc gacaagttta
     2461 cctacgccct cagcaattac gacttcggcg ttagagtatg cattccatac tcccgctcgc
     2521 aatctaacga aatctcgcgg aaaaaacatt catcgttatg tcagtgtaaa gatgagtaag
     2581 aggattacgg tagaatctac tctagagtgt gatgcctgct atcactttga ttttgagcca
     2641 agtattgttc gcttttgcgc tcaaccgatt cgatttttat attatctcaa tggtcagtct
     2701 cactcctatg ttcctgactt tctagttcaa tttgatacca acgagtttgt tctatatgaa
     2761 gtaaagtcag cttatgctaa gaacaaacct gattttgatg ttgaatggga ggcgaaagta
     2821 aaagcagcaa ctgaactagg gctagaattg gagcttgttg aagagagtga tattagggat
     2881 acggttgtat taaataatct taagcgcatg catcgttatg cttcgaaaga tgagctgaat
     2941 aacgtacata actctctctt aaaaataata aagtacaatg gcgcccaatc tgcaagatgc
     3001 ttgggagaac agttgggttt aaaaggccga actgttttac caattttgtg cgatttgctg
     3061 tcaaggtgtt tactcgatac acgtttggat aagcctctat ctcttgaatc tcgatttgag
     3121 ttggccagtt atggctaaga aagggttctc aagtttccat agaaaagcag tctcttccca
     3181 agatacgctt gaatcgatag agcttgtctc tagcgctaat tgtctagaaa gtgttacgta
     3241 tcaagatata tcagcatttc ccgaaacaat tgcggtagag attaatttcc gattaagcat
     3301 tcttcgcttt ttagcgcgaa agtgcgaaac cattgtggcc aaatcaattg aaccacatcg
     3361 tgtagagcta cagcaaaact atagtagaaa aatacccagt gcaataacga tatatcgatg
     3421 gtggcttgct tttcgaaaat cagactacaa ccccattagc ttagcaccta atatcaagga
     3481 tagaggtaat agagagacaa aagtgtcaac agttgttgat tctattatgg aacaggcagt
     3541 tgaaagagtt atatctggac gaaaagtcaa tgttagctct gcatataaac gtgttcgacg
     3601 aaaagttcgt caatacaatc taactcatgg aacgaaatac acgtatccta agtacgaatc
     3661 tgtaagaaag cgagtaaaaa agaaaacccc atttgagtta ttagccgcag ggaaagggga
     3721 gagagtagct aagagagagt ttcgccgaat gggaaaaaag atcctcacgt ctagcgtgct
     3781 agagagggtt gaaatagatc acactgtcgt tgaccttttt gcagtacacg aagagtatcg
     3841 aatcccattg ggccgacctt ggcttactca attggttgat tgttacagta aagctgtaat
     3901 cggtttttat ttaggtttcg agcctcctag ctatgtgtcg gtttcccttg cacttaagaa
     3961 tgcaatacaa cgcaaagatg acttaatctc ctcgtatgaa tcgatcgaga atgaatggct
     4021 atgttatggc atcccagacc tactcgtaac tgataatggt aaagagtttt tgtcgaaagc
     4081 atttgatcaa gcatgtgaat cactattgat caatgtgcat caaaataaag ttgaaacgcc
     4141 cgacaacaaa cctcatgttg aacgtaacta cgggactatt aatacttctc tgttagacga
     4201 tttacctggg aaatccttca gccagtacct tcaaagagaa gggtacgact ctgtgggaga
     4261 agctaccctt acactcaatg agattagaga aatttactta atttggttgg tggatattta
     4321 tcataaaaaa cccaatcaga gaggcactaa ttgtcctaat gttgcgtgga aaaagggttg
     4381 tcaagaatgg gaaccagagg agttctctgg ttctaaagac gaattagact ttaaatttgc
     4441 tattgttgat tacaaacaac ttactaaagt agggataact gtctacaaag aactgagtta
     4501 tagcaatgac cgtttagctg aatatagagg gaagaaagga aaccataaag ttcagttcaa
     4561 gtataaccct gagtgtatgg cagttatttg ggtgttggat gaggatatga atgagtactt
     4621 tacagttaat gcgattgact acgaatatgc aagtagagta tcactttggc aacataaata
     4681 taacatgaaa tatcaagcag aactaaattc agcagaatat gatgaggaca aggaaattga
     4741 tgcagaaata aaaattgaag aaatcgcaga tcgttcaatt gttaagacta acaaaatcag
     4801 agctcggagg cgtggcgcta ggcatcaaga gaatagcgca agggctaagt caatcagtaa
     4861 tgcgaacccg gcctcgatac aaaaacatga agatgaaatc gttagtgcag ataatgacga
     4921 ttgggatatt gattatgtct gagaaatcgt caaatgagtg aaacgcgtga ggctcgtata
     4981 tcaagagcta aaagggcatt tgtatccaca ccgagcgtta ggaaaatctt gagttacatg
     5041 gatagatgta gagatctatc agacctagag tctgagccta catgcatgat ggtctatggt
     5101 gcttcgggtg taggtaaaac gaccgtcatc aagaaatact taaatcaaaa cagaagagag
     5161 tccgaagccg ggggcgatat aataccggtt ttgcatattg agttgccaga caatgcgaag
     5221 ccagtagatg cagcaaggga attgctggtt gaaatgggtg acccgctagc actttatgaa
     5281 actgacttag ctagattgac gaaaagactg actgaattaa tccctgcggt cggcgtgaag
     5341 ctgattatta tcgatgagtt ccaacatttg gtggaagaaa ggtcaaatcg ggttcttacc
     5401 caagtaggta attggctaaa aatgatactt aacaaaacga aatgtccaat tgttatattt
     5461 ggtatgccat actcaaaagt tgtactgcaa gcaaactcgc aacttcacgg gcgattttcc
     5521 attcaggttg aactgcgccc ctttagctac cagggaggta gaggtgtatt taaaactttt
     5581 ttggaatacc ttgataaagc cctacctttt gaaaaacagg ctggcttagc caacgaaagt
     5641 ttgcagaaaa aattgtatgc attctctcag ggaaacatgc gttcgttgag aaaccttatt
     5701 tatcaagcat ctatcgaagc aattgataat cagcatgaaa cgataaccga agaagatttc
     5761 gtttttgcat cgaagttgac atcgggcgat aaacccaact catggaaaaa tccttttgag
     5821 gagggtgttg aggtaacaga agatatgtta cgaccgccac caaaagatat tggttgggag
     5881 gactatttga gacattcaac cccgagagtg agtaaaccag gtagaaataa aaactttttc
     5941 gaataaccta ggctgctgcc accgctgagc aataaccatc ttagtatatt agttaagtat
     6001 aagaaggaga tatacatatg tttttgcaaa gacctaaacc ttacagcgat gaaagtttag
     6061 aaagtttctt tatccgagtg gctaacaaaa atggctacgg tgatgtccat cgcttcctag
     6121 aagccactaa acgattcctt caagacattg accataatgg ctatcaaacc tttccgactg
     6181 atataactcg gataaaccca tactcagcta aaaacagttc cagcgcacga actgcgtcat
     6241 tcctgaaact tgcacaattg acatttaatg aaccgccaga gctacttggg ttggcaatta
     6301 acagaacaaa catgaaatac tcgccgtcaa ctagcgcggt tgttagaggt gcagaagtct
     6361 ttcctcgcag tttactacgg acgcactcca tcccctgctg tcctttgtgt ctgcgagaaa
     6421 atggctacgc ctcctacctt tggcactttc aggggtacga atactgccac agccataacg
     6481 tacctttaat taccacttgt agctgtggta aggagtttga ctaccgagta tctgggttaa
     6541 agggcatttg ctgcaaatgc aaggagccta tcaccttaac cagcagggag aacggtcatg
     6601 aggcagcgtg tactgtttca aactggcttg ctggccatga atctaaacct ctgccaaatc
     6661 ttcctaaaag ctaccgatgg ggtttagttc attggtggat gggtattaaa gatagcgagt
     6721 tcgatcactt ttcgttcgtt caatttttct caaactggcc aaggtcattc cactcgataa
     6781 tcgaagatga agtagagttc aaccttgagc atgctgttgt cagcacgtct gaattacgac
     6841 taaaagatct tcttggtcga ttgtttttcg gttcaattcg gttacctgag cggaatcttc
     6901 aacacaatat catccttggt gagcttctct gctatttaga aaatcgttta tggcaagaca
     6961 agggattaat cgccaacctc aaaatgaacg cgttagaggc gactgtaatg ttaaattgta
     7021 gcctcgatca gattgcatca atggttgaac aacgcatctt gaagccaaat cgaaaaagca
     7081 agcccaacag ccctcttgat gttaccgatt atctatttca tttcggcgat attttctgtc
     7141 tttggttagc tgagttccaa agcgatgagt ttaaccgttc gttttatgtg tcgaggtggt
     7201 aaatgcaaac tctgaaagaa ctaatcgcat ccaatcctga cgacttaaca actgaactta
     7261 agagagcatt tcgtccactc acaccgcata ttgcaattga tggtaatgaa cttgacgcac
     7321 tgacgatatt agtcaattta accgataaga ctgatgatca gaaagacctg ctcgatcgag
     7381 ccaaatgcaa gcaaaaactt cgagatgaaa aatggtgggc tagctgcata aattgcgtta
     7441 attacagaca aagccataac ccaaaattcc cggatatacg ttctgaaggc gtgattcgaa
     7501 cccaagccct gggtgaatta ccgagtttcc tactctcgtc ctcaaaaatc ccaccatacc
     7561 attggtcata tagccatgat tcaaaatacg tcaacaaaag cgcattcctc accaatgagt
     7621 tttgttggga tggtgagatc tcatgtttgg gtgagcttct taaagatgca gatcacccac
     7681 tttggaatac tttgaaaaag ttaggttgtt ctcaaaaaac ttgcaaagca atggcaaaac
     7741 aactagctga tattactctc acgactatca atgtcacact cgcaccaaat tacctgactc
     7801 aaatatctct ccccgatagt gatacatcct acatttcact ctctcctgtt gcatcgctat
     7861 cgatgcaaag ccactttcat cagaggctcc aagatgaaaa tcggcatagc gcgataacgc
     7921 ggtttagccg aactaccaac atgggggtca ctgcgatgac atgtggcggc gcatttagaa
     7981 tgttaaagtc tggcgctaag ttttccagcc cccctcatca ccgattaaac agtaaacgaa
     8041 gttggttgac gtcagagcat gttcagtcat taaaacagta ccagcgcctc aacaaaagcc
     8101 tcatacctga aaactctcgg attgcactcc gtagaaaata caaaatcgag cttcaaaata
     8161 tggtcagatc ttggtttgca atgcaagacc atacccttga ttcgaacata cttatccaac
     8221 atttgaatca tgacctatct tacttaggag ccacaaaacg ttttgcatac gatccagcga
     8281 tgaccaagct ctttactgag cttttgaaac gagagttatc aaattcaatc aataatggtg
     8341 agcaacacac taatggatcg tttttagtcc taccgaatat cagggtttgt ggcgcaacag
     8401 ctttaagctc cccggtaacg gtggggattc catcacttac agctttcttt ggcttcgttc
     8461 acgcatttga acggaatata aatcgcacca cctcatcgtt tcgtgttgaa tcctttgcga
     8521 tatgcgtcca tcaactacat gtcgagaagc gaggtttgac agcagagttt gtggaaaaag
     8581 gcgacgggac tatatccgct cccgcgaccc gggatgactg gcagtgtgat gtcgtattta
     8641 gccttatttt gaacaccaac tttgctcaac atattgacca agatacgtta gttacatcac
     8701 taccaaagcg attggctcgg ggttcagcaa aaattgcgat tgatgacttt aaacatatca
     8761 actcattctc gacattagaa acagcgatcg aatctctgcc aatagaagct ggtaggtggt
     8821 tatcacttta cgcacagtca aacaataatc taagtgatct attagcagcc atgacagagg
     8881 accatcagct catggcaagc tgcgtcggtt accacttgtt agaagaaccc aaagataaac
     8941 caaactccct cagaggttac aaacacgcta tcgccgagtg catcattgga ctcattaact
     9001 caatcacctt tagctcagag actgatccca acacaatctt ttggtcgcta aagaactatc
     9061 aaaactacct agtggtacag ccaaggagta tcaacgatga aactaccgac aaatctagcc
     9121 tatgagcgct ctatcgaccc atcagatgtc tgtttttttg tcgtctggcc cgatgataga
     9181 aaaacacctt taacctacaa ttctcgtact ctgctcgggc aaatggaagc ggcatcatta
     9241 gcctatgatg tctcaggtca accaataaaa agtgccaccg ctgaggcgtt agctcaaggg
     9301 aaccctcatc aagttgattt ctgccacgtt ccatacggtg cgagtcatat tgaatgcagt
     9361 ttctccgttt cgttttcttc tgaactacgt caaccatata agtgtaactc aagcaaagtt
     9421 aaacaaacgc tagtgcaatt agtcgagctc tacgaaacga aaatcggctg gactgagcta
     9481 gcaacccgat atttgatgaa catttgcaac ggtaaatggc tgtggaaaaa tacccgtaaa
     9541 gcctattgct ggaacattgt acttacacct tggccttgga acggggaaaa ggttggattt
     9601 gaagatattc gtactaacta cacctcacgg caagacttta aaaataataa aaattggtct
     9661 gctatagttg aaatgatcaa aaccgcattt tctagtactg atgggctggc gatatttgaa
     9721 gtcagggcca ccttgcactt gccaacgaat gctatggtgc ggccaagcca agttttcaca
     9781 gaaaaagaaa gtggcagtaa aagtaaatct aaaactcaaa acagtcgagt ttttcagagt
     9841 acaactattg atggtgaacg atcgccaata ctaggggcct ttaaaacggg agcagctatt
     9901 gcaaccattg acgactggta tcctgaagcc actgagccac taagggtcgg acggtttggg
     9961 gttcatcgcg aagatgtcac ttgctaccgt catccgtcta ccggaaaaga ttttttctcg
    10021 atattacaac aagcagagca ctatattgaa gtgttgagcg ccaacaaaac tcccgctcaa
    10081 gaaactatca acgacatgca ctttttaatg gctaacctga ttaagggtgg gatgttccag
    10141 cataaaggag actgactgtg aaatggtatt ataagacaat cacctttctg ccagagttgt
    10201 gcaacaacga gtcactggct gcaaagtgtc tccgcgttct gcatggattt aactatcagt
    10261 atgagacacg aaatatcggc gtttcatttc cgctttggtg tgatgcaacg gttggaaaaa
    10321 agatttcatt tgtcagcaag aacaagatag aactcgactt actacttaaa caacactatt
    10381 tcgtccaaat ggaacaactt caatactttc atatatccaa cactgttctc gtcccagaag
    10441 attgtacata cgtttccttt agacgctgtc aatctataga taagctcaca gcagcagggc
    10501 tggcaaggaa aatcagacgc ctggagaaac gtgctctatc tagaggcgag caatttgacc
    10561 catcatcttt tgctcaaaaa gagcatactg caatagcgca ctaccactca cttggggagt
    10621 ccagcaaaca gacgaaccgc aactttcgac tcaatatcag gatgctctcg gagcaacccc
    10681 gtgaggggaa ctcgattttt agtagctatg gcttatcaaa ttcagaaaac tcgtttcagc
    10741 ctgtaccctt aatctgacct aggctgctgc caccggtgaa ctgccgagta ggtagctgat
    10801 aacccctttc gccagctggc gtaatagcga agagggtgaa ctgccgagta ggtagctgat
    10861 aacccaaacc agatgtcaac acagctactg ttgatgcaac cataaagtga tatttaataa
    10921 ttatttataa tcagcaactt aaccacaaaa caaccatata ttgatatctc acaaaacaac
    10981 cataagttga tatttttcag ccaggggtcc ccaataatta cgatttaaat ttgacataag
    11041 cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
    11101 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
    11161 gtttttttgt acagcctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
    11221 gatgtttgat gttatggagc agcaacgatg ttacgcagca gcaacgatgt tacgcagcag
    11281 ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg cacgtgtagg
    11341 ctcggccctg accaagtcaa atccatgcgg gctgctcttg atcttttcgg tcgtgagttc
    11401 ggtgacgtag ccacctactc ccaacatcag ccggattccg attacctcgg gaacttgctc
    11461 cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc
    11521 gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg aaatctatat ctatgatctc
    11581 gcagtctccg gagagcaccg gaggcagggc attgccaccg cgctcatcaa tctcctcaag
    11641 catgaggcca acgcgcttgg tgcttatgtg atctacgtgc aagcagatta cggtgacgat
    11701 cccgcagtgg ctctctatac aaagttgggc atacgggaag aagtgatgca ctttgatatc
    11761 gacccaagta ccgccaccta acaattcgtt caagccgaga tcggcttccc ggccgcggag
    11821 ttgttcggta aattggacaa cggtccgcgc gttgtccttt tccgctgcat aaccctgctt
    11881 cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt
    11941 ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgaaagtgaa
    12001 acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt atttaataat gtgtgcggca
    12061 attcacattt aatttatgaa tgttttctta acatcgcggc aactcaagaa acggcaggtt
    12121 cggatcttag ctactagaga aagaggagaa atactagatg cgtaaaggcg aggagctgtt
    12181 cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc ataagttttc
    12241 cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga agttcatctg
    12301 tactactggt aaactgccgg ttccttggcc gactctggta acgacgctga cttatggtgt
    12361 tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca agtccgccat
    12421 gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca cgtacaaaac
    12481 gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc tgaaaggcat
    12541 tgactttaaa gaggacggca atatcctggg ccataagctg gaatacaatt ttaacagcca
    12601 caatgtttac atc
//
