LOCUS       sequence-371401-        8588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses LaminA with C-terminal tags ECFP and SNAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Vester K, Preussner M, Holton N, Feng S, Schultz C, Heyd F, Wahl MC
  TITLE     Recruitment of a splicing factor to the nuclear lamina for its 
            inactivation.
  JOURNAL   Commun Biol. 2022 Jul 22;5(1):736. doi: 10.1038/s42003-022-03689-y.
  PUBMED    35869234
REFERENCE   2  (bases 1 to 8588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-07-22"; volume: "5(1):736. doi"; pages: " 
            10.1038/s42003-022-03689-y"
COMMENT     Sequence Label: LaminA-ECFP-SNAP
FEATURES             Location/Qualifiers
     source          1..8588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2928..2937
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2934..2936,2937..2939,2940..3650)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2979..3000)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3240..3259)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3587..3608
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3708..4253
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     intron          4364..4593
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4576..4595
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(4666..5240,5241..5243,5244..5252)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4833..4850)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5060..5079
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5275..6078
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5338..5357)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5948..5967
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6208..6329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6245..6264)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6431..6447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6431..6447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6444..6466)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6455..6471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6479..6485,6486..6503,6504..6509))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6524..6545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6662..6679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6833..7421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6913..6932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7592..8383,8384..8452))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8215..8234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8453..8557)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cgtcgctagc accatggaga ccccgtccca gcggcgcgcc acccgcagcg
      961 gggcgcaggc cagctccact ccgctgtcgc ccacccgcat cacccggctg caggagaagg
     1021 aggacctgca ggagctcaat gatcgcttgg cggtctacat cgaccgtgtg cgctcgctgg
     1081 aaacggagaa cgcagggctg cgccttcgca tcaccgagtc tgaagaggtg gtcagccgcg
     1141 aggtgtccgg catcaaggcc gcctacgagg ccgagctcgg ggatgcccgc aagacccttg
     1201 actcagtagc caaggagcgc gcccgcctgc agctggagct gagcaaagtg cgtgaggagt
     1261 ttaaggagct gaaagcgcgc aataccaaga aggagggtga cctgatagct gctcaggctc
     1321 ggctgaagga cctggaggct ctgctgaact ccaaggaggc cgcactgagc actgctctca
     1381 gtgagaagcg cacgctggag ggcgagctgc atgatctgcg gggccaggtg gccaagcttg
     1441 aggcagccct aggtgaggcc aagaagcaac ttcaggatga gatgctgcgg cgggtggatg
     1501 ctgagaacag gctgcagacc atgaaggagg aactggactt ccagaagaac atctacagtg
     1561 aggagctgcg tgagaccaag cgccgtcatg agacccgact ggtggagatt gacaatggga
     1621 agcagcgtga gtttgagagc cggctggcgg atgcgctgca ggaactgcgg gcccagcatg
     1681 aggaccaggt ggagcagtat aagaaggagc tggagaagac ttattctgcc aagctggaca
     1741 atgccaggca gtctgctgag aggaacagca acctggtggg ggctgcccac gaggagctgc
     1801 agcagtcgcg catccgcatc gacagcctct ctgcccagct cagccagctc cagaagcagc
     1861 tggcagccaa ggaggcgaag cttcgagacc tggaggactc actggcccgt gagcgggaca
     1921 ccagccggcg gctgctggcg gaaaaggagc gggagatggc cgagatgcgg gcaaggatgc
     1981 agcagcagct ggacgagtac caggagcttc tggacatcaa gctggccctg gacatggaga
     2041 tccacgccta ccgcaagctc ttggagggcg aggaggagag gctacgcctg tcccccagcc
     2101 ctacctcgca gcgcagccgt ggccgtgctt cctctcactc atcccagaca cagggtgggg
     2161 gcagcgtcac caaaaagcgc aaactggagt ccactgagag ccgcagcagc ttctcacagc
     2221 acgcacgcac tagcgggcgc gtggccgtgg aggaggtgga tgaggagggc aagtttgtcc
     2281 ggctgcgcaa caagtccaat gaggaccagt ccatgggcaa ttggcagatc aagcgccaga
     2341 atggagatga tcccttgctg acttaccggt tcccaccaaa gttcaccctg aaggctgggc
     2401 aggtggtgac gatctgggct gcaggagctg gggccaccca cagcccccct accgacctgg
     2461 tgtggaaggc acagaacacc tggggctgcg ggaacagcct gcgtacggct ctcatcaact
     2521 ccactgggga agaagtggcc atgcgcaagc tggtgcgctc agtgactgtg gttgaggacg
     2581 acgaggatga ggatggagat gacctgctcc atcaccacca cggctcccac tgcagcagct
     2641 cgggggaccc cgctgagtac aacctgcgct cgcgcaccgt gctgtgcggg acctgcgggc
     2701 agcctgccga caaggcatct gccagcggct caggagccca ggtgggcgga cccatctcct
     2761 ctggctcttc tgcctccagt gtcacggtca ctcgcagcta ccgcagtgtg gggggcagtg
     2821 ggggtggcag cttcggggac aatctggtca cccgctccta cctcctgggc aactccagcc
     2881 cccgaaccca gagcccccag aactgcagca tcatgggtac cgagctcgcc accatggtga
     2941 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3001 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3061 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3121 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     3181 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3241 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3301 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3361 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     3421 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     3481 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     3541 gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     3601 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
     3661 gatctcgagc tcaagcttcg aattctgcag gtaccggtga attcaccatg gacaaagact
     3721 gcgaaatgaa gcgcaccacc ctggatagcc ctctgggcaa gctggaactg tctgggtgcg
     3781 aacagggcct gcaccgtatc atcttcctgg gcaaaggaac atctgccgcc gacgccgtgg
     3841 aagtgcctgc cccagccgcc gtgctgggcg gaccagagcc actgatgcag gccaccgcct
     3901 ggctcaacgc ctactttcac cagcctgagg ccatcgagga gttccctgtg ccagccctgc
     3961 accacccagt gttccagcag gagagcttta cccgccaggt gctgtggaaa ctgctgaaag
     4021 tggtgaagtt cggagaggtc atcagctaca gccacctggc cgccctggcc ggcaatcccg
     4081 ccgccaccgc cgccgtgaaa accgccctga gcggaaatcc cgtgcccatt ctgatcccct
     4141 gccaccgggt ggtgcagggc gacctggacg tggggggcta cgagggcggg ctcgccgtga
     4201 aagagtggct gctggcccac gagggccaca gactgggcaa gcctgggctg ggtcctgcag
     4261 gcggatccgc gtttaaactc gaggttaatt aatgagcggc cgcatagata actgatccag
     4321 tgtgctggaa ttaattcgct gtctgcgagg gccagctgtt ggggtgagta ctccctctca
     4381 aaagcgggca tgacttctgc gctaagattg tcagtttcca aaaacgagga ggatttgata
     4441 ttcacctggc ccgcggtgat gcctttgagg gtggccgcgt ccatctggtc agaaaagaca
     4501 atctttttgt tgtcaagctt gaggtgtggc aggcttgaga tctggccata cacttgagtg
     4561 acaatgacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcagg
     4621 tcgagcatgc atctagggcg gccaattccg cccctctccc ccccccccct ctccctcccc
     4681 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     4741 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     4801 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     4861 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     4921 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     4981 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     5041 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     5101 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     5161 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     5221 ttgaaaaaca cgatgataag cttgccacaa cccgggataa ttcctgcagc caatatggga
     5281 tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     5341 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     5401 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     5461 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     5521 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     5581 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     5641 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     5701 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     5761 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     5821 gacggcgatg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     5881 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     5941 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     6001 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     6061 cttgacgagt tcttctgagg ggatcaattc tctagataac tgatcataat cagccatacc
     6121 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     6181 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     6241 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6301 ggtttgtcca aactcatcaa tgtatcttaa cgcgtcgagt gcattctagt tgtggtttgt
     6361 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     6421 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6481 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6541 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6601 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6661 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6721 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6781 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6841 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6901 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6961 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7021 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7081 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7141 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7201 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7261 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7321 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7381 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7441 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7501 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7561 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7621 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7681 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7741 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7801 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7861 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7921 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7981 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8041 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8101 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8161 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8221 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8281 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8341 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8401 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8461 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8521 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8581 ctgacgtc
//
