LOCUS       sequence-371411-       13750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for Tol2 transgenesis expressing APEX2 tagged to 
            mitochondria and nuclei, and cytoplasmic mCherry, driven by the 
            draculin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13750)
  AUTHORS   Agarwala S, Kim KY, Phan S, Ju S, Kong YE, Castillon GA, Bushong EA,
            Ellisman MH, Tamplin OJ
  TITLE     Defining the ultrastructure of the hematopoietic stem cell niche by 
            correlative light and electron microscopy.
  JOURNAL   Elife. 2022 Aug 9;11. pii: 64835. doi: 10.7554/eLife.64835.
  PUBMED    35943143
REFERENCE   2  (bases 1 to 13750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-9"; volume: "11. pii: 64835. doi"; pages: " 
            10.7554/eLife.64835"
COMMENT     Sequence Label: Tol2pA2-drl:APEX2-mCherry
FEATURES             Location/Qualifiers
     source          1..13750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    7497..7521
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7612..7653
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7654..8400
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             8410..8466
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8470..8493
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9271..9645
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     CDS             9655..9711
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9712..10422
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9857..9875)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10125..10144
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     10319..10342
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(10424..10448)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    10450..10584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(10647..10667)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10675..10691)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    10707..10841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(11280..11467,11468..11479)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11513..11529)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11513..11529)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(11566..11586)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11566..11584)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11605..11621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11618..11640)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11629..11645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11653..11659,11660..11677,11678..11683))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11698..11719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11836..11853)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12007..12595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12087..12106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12766..13557,13558..13626))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13389..13408
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13627..13731)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggct ccgaattcgc ccttgtcagc
     1141 accagatgcc tgtgctgata agtaaagacc gaacaaacgt atccacacag ctctaatcta
     1201 ctccttacaa gatgtttcct ccaccagtca tttcaacgtg accagtgaag cacacactat
     1261 aggtgaagtc taatacattt agagaaagta cagttaaaga aacagatcag cactttgcaa
     1321 atactaactt taaaaaaaat aaaaacaact cctgaaacaa attcaaaggc ataaataaca
     1381 tatttcaata gtcaaactac agaaaagtac atttagtctt tagaacgtat atacaaaaca
     1441 ggaaaatgag tatagcatat cctagtaata tgaatgctta aaagtaaatt taaaaatgga
     1501 ataatatata attaattatt atagaaaata caacaccttt acacatttgc atacaaaacc
     1561 tttcacattt tttagatttg tcccatttaa atgcacctta tttaaacatc aaacatacat
     1621 tttattatta gacacattta aatatgtttt aataagatta ctaaaatgtt ttaatgttat
     1681 tattcattca ttcattttct tttcggctta gtcgctttat taatctgggg tcgccaatgc
     1741 ggaatgaacc accaacttat ccagcacgtt tttacgccgc agatgccctt cctgcccaaa
     1801 acccatcact gggaaacatc cacctagcac ccagaggaaa cccactcgaa agcggggaga
     1861 acatgcaaac tccacacaga aatgccaact gacccagcgg aggctcgaac tagcgacctt
     1921 cttgctgtga ggcgacagca ctacctactg tgccaccatg tctccgtttt aatgttatcc
     1981 tgtctaaaat atttgtaatt catttttaca atatgttaca agtttatttg tgatttcatt
     2041 cttttttaat gcagatctcc tttaaatata ttttgtctgt atggctggcc gtcagaaatg
     2101 tctgtattta ttggacacaa gaatatgttt tttaagaatt attgctgatt gattgatcag
     2161 ccatgagtac tgtatttgtg tttaaatgaa aacataagac aacagagaaa aaagaagggg
     2221 gaaaaaaaca ttttggagac tttaaagatt ctgtttattg aatttgtagc attttagtaa
     2281 taataaaatt aataaaaaac tttatttacc gtttaaatcc attcaaagac attcattaaa
     2341 agcatgatta tgataatttt aagtatttta aaatatacac acaaggtcaa atacagtctg
     2401 cggtagctgt agaaagatat agctgaaata taaaatattt aatgaaactg ataatctttt
     2461 ggaaatttta ggggagaaaa cttttctcat ttttttaaac ctaactttag attcatttta
     2521 ttgatcgcag cagttccgtg tgtctttggc acattattat tcattcattc attttctttt
     2581 ggcttagtca ctttattaat caggggtcac cacagcggaa tgaactgcca acttatccag
     2641 cgtacagtat gttttatgca gcagattccc ttccagcaac aacccataca ctctcattca
     2701 caaacacaca tcattcacta cggccaattt agctaaccca agtcatatag tgcatgtctt
     2761 tggactgagg gggaaaccgg agcacctgga ggaaacccac accaacacag ggagaacatg
     2821 caaactccac acagaaatgc caaataaccc agccgaggct taaaccagag accttcttgc
     2881 tgtgaagcga ttgttctacc cactgcgcca ccccaccacc cattattatt aatattatat
     2941 tatttctatc aatattacta tcaaatattt tcttgaaagt aatccatata cttcagctta
     3001 aagtttattc tttatactta attaaagtct gaaaattaaa gtataaaaat aagcagaagg
     3061 atcaaccttc aatattgtat ggaaaaaaaa gaacgacaga aaacaaagga gggatttttg
     3121 gagactgagc gttttgttta taattatata gtgctaaatt gataatgatt acaccaaaaa
     3181 atgtttttca ctgtttattg ctgtttaatt tactgtttaa atgtattaaa taaaaaaaac
     3241 tacttttatc agcaccatgt gatacatgta aaagtaacta gcttccttta gctgttgttt
     3301 ttttttaatt tagaagaaaa aaaaactagc atgaacctca attataacca cttatttttg
     3361 tttactttat ttttatccac taaaatcttt tacagaacaa aacattcagt gacagcatcg
     3421 attattacaa ataaagacag aaaaaataaa gtaacactag atgtaaataa aaataaatta
     3481 caaaacataa aaaaacatac atacattatt ttcaatttca aaggagctgg aaatactatg
     3541 tcctgttcag agatatagta ataagtaccc atctttgttt aaatatgcaa tttcaattgt
     3601 ggttgagcag tcataaactt cattttatat atatagatat ataaggtaga tatatatata
     3661 tattcaagtt tttctatccg ctgttctgtt gttggagggt gtggcttcat ccaatttatt
     3721 gttatcgtct tttttgcact aagtaaaagt atatgtagta tatatatttc tggtttttaa
     3781 tatatacatc ccggggtata ctttcaatca aataaaacag gggagtaaaa ggtagatcta
     3841 actgcataat tttcattatc ttaatcttta ccccttgcca aaatgcaagt agcttcagac
     3901 attctaacca cttgtctttt aaacatgatt tcagtcttat ttggctttgg ttgtgttaca
     3961 ttcttcatat attcttcatt cttcagattc tgtcatcatt cactcaccct tcactaatag
     4021 tgtatttctt ctgttgaaca caaaaccgct acattctata aattatgttt gttcaaacta
     4081 cttgtttgta aaaagctaaa ataacacaat tcttgaggtt tttgtgacaa ctaaattgtt
     4141 ttgtgttaaa tccactttgt aaacattaat cagttaactt aattccataa ttcgaatgtc
     4201 atttatttta cagtgtatcc agtgtagcag ctcaatggtt acaagtttcc aacattcttc
     4261 aaaacgtctt cttttggttt taacaaaaaa aaaaaaaaaa gaaactcaga aaggtttgaa
     4321 acaacccaag attgattaaa tgtaaatttt tggccaactc ttcccttaag attaggagtg
     4381 gcgttttaac ggtttgggct tgagggggct tttcttcagg ggggctataa gagcttaaaa
     4441 gcactgatct gtgaagaaac gagtatcagt aggatgcaga cgattgatag tgcagccatt
     4501 gcaaaacttc accctaaccg actcactcaa aacacagaat cattcagtaa ggaactgctg
     4561 tgtgttgctc agagacaatt ttgcatctaa aatgtaagta acttattatt tctttttgtg
     4621 tattaaactg ttgtgtaaaa tcaatgtcat gctttacact tgtgctagaa taacagcact
     4681 cttgctcatg tgtgattgct taactaatgt tcagtgattt ttttaaacct taaccttgtt
     4741 attctgacct gcttgttgtt aaagggtata taatataaat tatatttttt ttataataac
     4801 cctgattaac cctgttaatc atccagacat caaccacatt tctttcatat ttaaattcct
     4861 attggagatg catagcagga taatctccca ttcacgagtc ttctatgcag agaaattttc
     4921 tcaggtcttt gagtaatgca tttagacaat aatgtccaaa cttatgttta aataagtttt
     4981 tgttgttaca gatgaaatat aacatggttt aaatatgtcc cagttggcta gatattaatt
     5041 aacagtcatt caaacaactt aaacctctct acttcacggt tggtcccgca catccacaaa
     5101 ttttgtagtt tatttacact ttttacccgc gtttttagtt ctaaccaaaa tcacgtccaa
     5161 ttccaacaca gggtaaaatg ttaaggtatt tgctcagggt taaccatggc attttcaagg
     5221 tttttaccac aatatcggca gacgtcttcc tgccaattat tgtaaaagtt cggcaaaatt
     5281 tggatgagaa acacaggaaa aagtattttt attacagtct atgttttttt atttaaaaaa
     5341 aaatctgtga aagtatgcaa gtaacagcta atattgttgt gattaattat gtcttctagg
     5401 ctaaaataca cacaatgact gctagccagt tttgggaaaa caacaaaata attgttgaat
     5461 tttgggtgta atgttgggaa ctttttaaat attttttttt aatacataaa atatttttta
     5521 ataaaagaaa attgttttat tgctcataat atttcaaata aaggcattgt ataacaatag
     5581 ataataatgc aaataaatac taatgtgttt atttaataga taaatactta attaaaccca
     5641 ctgtgctgtg tggaataaga agaaattagc tactgaggaa atacacaaaa taaagtaaac
     5701 taaatttcaa ttaattgagt gaaatctgac ttttaggcaa ctgttacaac ctgccccaca
     5761 ggtggggtaa aaagtacaat ttttattact ggcacatttg gcaatactgc acgaaagatc
     5821 cagcgatcat actttcagtg ctcataggag aggcttgcgt gttgttggta aaaaaaaatg
     5881 gtaacaccgt tactttgttc attatttggc caaaaccaaa aagtgttact ttgtgccccg
     5941 ctctctccta cagtgggcaa tattagcggt taaagaatgg acgcttttac gctatgaatt
     6001 ttactggaaa taataaatca tctgggaacc tttgggcctg ggcacgcttg cgtcattaat
     6061 gcagcactgt tcagttaggg ctcttcctca gcggagattt cttgagttat ataatttaag
     6121 ttgtttaata tgcggtgaca cgcagtcaga tatttcgcta aacagatcag ccacttttga
     6181 cgctcataaa cattcataaa ttcctcatgc tgcagaaatt aggaggtttg ctgaaagtgc
     6241 gcagcggtca tgcagtgagg ggtttgcgtc tttaataaac tacgacagtt tgcgttcaat
     6301 aaacagtaag aatgattaat aaattaatta attaagttct gtgaatgaac gtcgcctcgt
     6361 ggttccatcc caaagaggga agaaatcact ttcccgaacg ctcgcgttca atctgcccag
     6421 ttggtggaat gaactcccta actgcatcag aacggcagag tcactcgctg tcttcaagaa
     6481 acgactaaaa actcaactat ttagtctcca cttcacttcc taatctgcta ttgcctctct
     6541 gtacatacca ctaacagtac tcaaaaaaat aaataaaaaa ataaaatttt tactaatgct
     6601 ttccttctta gacttgacag acctgaaact tgcctatagc acttattcat tgttgctgtt
     6661 gtagttgtgt agattgcttc cttgtcctca tttgtaagtc actttggata aaagcatctg
     6721 ctaaatgact aaatgtaaat ctaaagctag aactttcaat gcagcaaatt gcgaaacttg
     6781 ctgcacgacc ttacgtttct gtgctgccgc attcacatgc gtataaatga aagtctaggg
     6841 agaaaagtgc agtgtgaccg cagctttaat ccttataaag ataccatatt gcaggtcaaa
     6901 tcaatcaatg cggtgctctc atatttcctg tgattggctg ttcggtgtca cactttcagc
     6961 tgcatgcagt cctgtgttaa tcacaaagtc tgggttaaca gagaatgttt ataaaatgtt
     7021 tataagcatt gtgaaacaac ttaacctgat cttaaccagg ctgaatttgt ttgatttttg
     7081 tcaatccaga gtttgttcaa ctcattttgt gcaacaggct tctggtattg cagaatgtat
     7141 ttttcccgaa gctgcagggg gtttagtagg tgggtaaaca caaatctcaa tgtcaatagg
     7201 tgttaaatcg gcatgtctgt tatcattaaa cagcagggaa cttcacactg ctccagtgat
     7261 ctgtttaaag atttgtttag tcagtgtctg gaaacagtct gaatctggtg gtttaattgt
     7321 ctttggtttt aatgtcctct tcgcagattg taggttgact gagagacatt taggtgtgat
     7381 aaagctgtga tctatggctt gttgttgtaa atttctttgt tgtaaacttg ttgatttgtt
     7441 tatttcagaa ctaccactca acgatcccaa ttcacacttg gaagggcgaa ttcgacccaa
     7501 gtttgtacaa aaaagcaggc tggacccacc atgctggcca cccgcgtgtt cagcctggtg
     7561 ggcaagcgcg ccatcagcac cagcgtgtgc gtgcgcgccc acaaggatcc aggcaagccc
     7621 atccccaacc ccctgctggg cctggacagc accggaaagt cttacccaac tgtgagtgct
     7681 gattaccagg acgccgttga gaaggcgaag aagaagctca gaggcttcat cgctgagaag
     7741 agatgcgctc ctctaatgct ccgtttggca ttccactctg ctggaacctt tgacaagggc
     7801 acgaagaccg gtggaccctt cggaaccatc aagcaccctg ccgaactggc tcacagcgct
     7861 aacaacggtc ttgacatcgc tgttaggctt ttggagccac tcaaggcgga gttccctatt
     7921 ttgagctacg ccgatttcta ccagttggct ggcgttgttg ccgttgaggt cacgggtgga
     7981 cctaaggttc cattccaccc tggaagagag gacaagcctg agccaccacc agagggtcgc
     8041 ttgcctgatc ccactaaggg ttctgaccat ttgagagatg tgtttggcaa agctatgggg
     8101 cttactgacc aagatatcgt tgctctatct gggggtcaca ctattggagc tgcacacaag
     8161 gagcgttctg gatttgaggg tccctggacc tctaatcctc ttattttcga caactcatac
     8221 ttcacggagt tgttgagtgg tgagaaggaa ggtctccttc agctaccttc tgacaaggct
     8281 cttttgtctg accctgtatt ccgccctctc gttgataaat atgcagcgga cgaagatgcc
     8341 ttctttgctg attacgctga ggctcaccaa aagctttccg agcttgggtt tgctgatgcc
     8401 ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct
     8461 ggacctatgg actacaagga tgacgacgat aaggggaaat catacccaac agtgtccgca
     8521 gactaccagg atgccgtgga gaaagccaag aagaagctga gagggtttat cgcagaaaag
     8581 aggtgcgcac ctctgatgct gagactggct ttccacagcg caggcacctt tgacaaggga
     8641 accaaaacag gcggaccctt tggaacaatc aagcaccctg ctgaactggc acatagtgcc
     8701 aacaatgggc tggacatcgc cgtgcggctg ctggaacctc tgaaagcaga gttcccaatt
     8761 ctgtcctacg ccgattttta tcagctggca ggagtggtcg ctgtggaggt cactgggggc
     8821 cccaaggtgc ctttccaccc aggacgggag gacaaaccag aaccacctcc agaggggcgc
     8881 ctgccagatc cgacaaaggg ctccgaccat ctgcgagatg tgtttgggaa agctatgggc
     8941 ctgactgacc aggatatcgt cgcactgtct ggagggcaca ccattggcgc cgctcataag
     9001 gaaaggtcag gcttcgaggg accctggaca agcaaccccc tgattttcga caattcttac
     9061 tttactgaac tgctgagtgg agagaaggaa gggctgctgc agctgcccag cgacaaagcc
     9121 ctgctgtccg atcccgtgtt cagacctctg gtcgataagt atgcagccga cgaggatgct
     9181 tttttcgcag attacgcaga agcacatcag aagctgtcag aactgggatt tgccgacgcc
     9241 tccggactca gatctggagg ctccggaggc ccagagccag cgaagtctgc tcccgccccg
     9301 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag
     9361 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct
     9421 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc
     9481 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc
     9541 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc
     9601 gtgtccgagg gtactaaggc catcaccaag tacaccagcg ctaagggaag cggagctact
     9661 aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc tatggtgagc
     9721 aagggcgagg aggacaacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     9781 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     9841 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
     9901 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     9961 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
    10021 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
    10081 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
    10141 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
    10201 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc cgaggtcaag
    10261 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
    10321 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgcgccgag
    10381 ggccgccact ccaccggcgg catggacgag ctgtacaagt aagacccagc tttcttgtac
    10441 aaagtggggg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
    10501 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
    10561 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
    10621 cagggggagg tgtgggaggt tttttccaac tttattatac atagttgata attcactggc
    10681 cgtcgtttta cggtaccatc gatgatgatc cagacatgat aagatacatt gatgagtttg
    10741 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
    10801 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
    10861 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
    10921 acaaatgtgg tatggctgat tatgatcctc tagatcagat ctgcgaagat acggccacgg
    10981 gtgctcttga tcctgtggct gattttggac tgtgctgctc gcagctgctg atgaatcaca
    11041 tacttcctcc attttcttcc actgattgac tgttataatt tccctaattt ccaggtcaag
    11101 gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact tccaaaggac caatgaacat
    11161 gtctgaccaa tttcatataa tgtgaaaacg attttcatag gcagaataaa taacatttaa
    11221 attaaactgg gcatcagcgc aattcaattg gtttggtaat agcaagggaa aatagaatga
    11281 agtgatctcc aaaaaataag tactttttga ctgtaaataa aattgtaagg agtaaaaagt
    11341 actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt ttaattgtac
    11401 tcaagtaaag taaaaatccc caaaaataat acttaagtac agtaatcaag taaaattact
    11461 caagtacttt acacctctgg ttcttgaccc cctaccttca gcaagcccag cagatccact
    11521 agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt
    11581 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    11641 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    11701 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    11761 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    11821 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    11881 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    11941 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    12001 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    12061 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    12121 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    12181 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    12241 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    12301 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    12361 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    12421 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    12481 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    12541 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    12601 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaaggcat
    12661 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    12721 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    12781 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    12841 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    12901 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    12961 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    13021 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    13081 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    13141 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    13201 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    13261 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    13321 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    13381 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    13441 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    13501 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    13561 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    13621 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    13681 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    13741 ccgaaaagtg
//
