LOCUS       sequence-371416-        9182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral backbone containing ZNF10 KRAB as a fusion with 
            rTetR(SE-G72P)-3XFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Tycko J, Van MV, Aradhana, DelRosso N, Ye H, Yao D, Valbuena R, 
            Vaughan-Jackson A, Xu X, Ludwig C, Spees K, Liu K, Gu M, Khare V, 
            Mukund AX, Suzuki PH, Arana S, Zhang C, Du PP, Ornstein TS, Hess GT,
            Kamber RA, Qi LS, Khalil AS, Bintu L, Bassik MC
  TITLE     Development of compact transcriptional effectors using 
            high-throughput measurements in diverse contexts.
  JOURNAL   Nat Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6.
  PUBMED    39487265
REFERENCE   2  (bases 1 to 9182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6."
COMMENT     Sequence Label: pJT151 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF10
            K...
FEATURES             Location/Qualifiers
     source          1..9182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        58..256
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     211..231
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             274..454
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    501..626
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1123..1356
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1541..1585
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1883..2000
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2123..2334
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2335..3273
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3230..3250
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3914..3979
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4028..4213
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             4223..4276
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4277..4984
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4422..4440)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4690..4709
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5003..5401
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(5421..5454)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5510..6098
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5563..5583)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5981..5992)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6101..6117)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6102..6118)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6627..6807
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6869..7457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6949..6968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7628..8419,8420..8488))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8251..8270
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8489..8593)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8668..8687)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8859..9182,1..56)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
       61 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      121 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      181 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      241 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
      301 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      361 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      421 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      481 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      541 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      601 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      661 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      721 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      781 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
      841 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
      901 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
      961 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1021 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1081 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1141 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1201 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1261 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1321 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1381 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1441 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1501 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1561 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1621 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1681 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1741 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1801 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     1861 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     1921 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     1981 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2041 taccgggccc gctctagaga tcccatttga ctcaggcggc cgcgtggata accgtattac
     2101 cgccatgcat gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     2161 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     2221 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     2281 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     2341 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     2401 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     2461 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     2521 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     2581 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     2641 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
     2701 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     2761 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     2821 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     2881 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     2941 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     3001 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     3061 gattagttct cgcgcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     3121 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     3181 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     3241 acagtggttc aaagtttttt tcttccattt caggtgtcgt gagctagcca ccatgtctag
     3301 actggacaag agcaaaataa taaacggagc tctggaatta ctcaatggtg tcggtatcga
     3361 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     3421 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
     3481 tcatacccac tcctgccccc tggaacccga gtcatggcaa gactttctgc ggaacaacgc
     3541 caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     3601 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     3661 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     3721 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     3781 cgattctatg cccccacttc tgaaacaagc aattgagctg ttcgaccggc agggagccga
     3841 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     3901 cgaaagcggc ggtgactaca aagaccatga cggtgattat aaagatcatg acatcgacta
     3961 caaggatgac gatgacaagg gtggatccgg atcaggctcc gatgctaagt ctctgactgc
     4021 ctggtccagg acccttgtga ccttcaagga cgtcttcgtt gattttacgc gggaagaatg
     4081 gaaactacta gacacagcac aacaaatagt atacagaaac gtaatgctag aaaactacaa
     4141 aaacctagta agcctaggat accaactaac aaaaccagac gtaatactaa gactagaaaa
     4201 aggagaagaa ccaggatctg gagagggaag gggaagcctc ctaacttgcg gagatgtcga
     4261 ggagaatcct ggcccaatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     4321 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     4381 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     4441 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     4501 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     4561 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     4621 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     4681 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     4741 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     4801 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     4861 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     4921 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     4981 caagggtagc ggcagcggta gcatggccaa gcctttgtct caagaagaat ccaccctcat
     5041 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     5101 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     5161 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     5221 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     5281 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     5341 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     5401 agaattcgtc gagggaccta ataacttcgt atagcataca ttatacgaag ttatacatgt
     5461 ttaagggttc cggttccact aggtacaatt cgatatcaag cttatcgata atcaacctct
     5521 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     5581 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     5641 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     5701 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     5761 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     5821 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     5881 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     5941 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6001 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6061 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgatc
     6121 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     6181 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     6241 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6301 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     6361 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     6421 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     6481 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     6541 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     6601 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6661 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6721 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6781 tttagtcagt gtggaaaatc tctagcagca tgtgagcaaa aggccagcaa aaggccagga
     6841 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6901 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6961 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7021 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7081 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7141 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7201 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7261 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7321 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7381 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7441 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7501 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7561 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7621 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7681 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7741 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7801 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7861 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7921 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7981 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8041 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8101 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8161 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8221 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8281 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8341 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8401 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8461 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8521 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8581 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8641 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8701 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     8761 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     8821 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     8881 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     8941 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     9001 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     9061 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     9121 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     9181 ct
//
