LOCUS       sequence-371424-       11576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HisSUMO tagged OphP to express OphP in pichia pastorias..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Emmanuel Matabaro, Haigang Song, Fabio Gherlone, Lukas Sonderegger, 
            Andrew Giltrap, Sam Liver, Alvar Gossert, Markus Kunzler, James H 
            Naismith
  TITLE     Molecular basis for the enzymatic macrocyclization of multiply 
            backbone N-methylated peptides
  JOURNAL   bioRxiv 2022.07.21.500988
REFERENCE   2  (bases 1 to 11576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.21.500988"
COMMENT     Sequence Label: HisSumoOphP
FEATURES             Location/Qualifiers
     source          1..11576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..937
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             956..979
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1277..1297
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      3629..3875
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(4288..6822)
                     /codon_start=1
                     /gene="Pichia pastoris HIS4"
                     /product="multifunctional enzyme, required for histidine 
                     biosynthesis"
                     /label=PpHIS4
                     /note="auxotrophic marker for Pichia pastoris"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     CDS             complement(7235..8050)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7235..8050)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7958..7977
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8042..8061
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(8215..8234)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    8429..9177
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     primer_bind     complement(9213..9235)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9321..9461
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9376..9395
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9476..9493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9647..10235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9727..9746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10406..11197,11198..11266))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11029..11048
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11267..11371)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11439..11457
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccgaaacta tgggtcatca
      961 tcatcatcat catcatcatg gtggttctga ctccgaggtc aaccaggagg ctaagcctga
     1021 ggttaagcca gaggttaagc ctgagaccca cattaacttg aaggtttccg acggttcatc
     1081 cgaaatcttc ttcaagatta agaagactac cccattgaga agattgatgg aggccttcgc
     1141 caagagacaa ggaaaggaga tggactcctt gactttcttg tacgacggta tcgagatcca
     1201 ggccgaccaa accccagagg acttggacat ggaggacaac gacatcatcg aggctcacag
     1261 agagcaaatt ggtggtgaga acttgtactt ccaaggtact agtatgtcgt ttccaggatg
     1321 gggaccatat ccacctgttg aaagggacga aacttcagcg ataacttaca gtagcaagtt
     1381 gcatggctct gttacggttc gtgatcccta cagtcaactt gaagtacctt tcgaggatag
     1441 tgaagaaacg aaggcgtttg ttcattcaca acgcaagttt gcacgaacct atctagatga
     1501 aaatcccgat cgagaagcat ggctcgagac gctgaagaag agctggaatt acaggagatt
     1561 tagcgcattg aaacctgaaa gcgacggaca ttactacttc gagtataacg atggccttca
     1621 atctcagctt tccctgtatc gtgtgaggat gggggaggag gataccgtcc tcactgagtc
     1681 tggtcctggt ggagaactct ttttcaatcc caacttgctt tcccttgatg gcaacgccgc
     1741 acttactggt ttcgtcatga gtccatgtgg gaattattgg gcatatggcg tatctgaaca
     1801 tggatccgac tggatgtcta tttacgttcg aaagacgtcg tctccgcatc ttccgtcaca
     1861 agaacgagga aaggatcctg gacggatgaa cgacaagatt cgacacgtcc gtttcttcat
     1921 tgtatcttgg acaagtgaca gcaaaggttt tttctattct aggtatcctc ctgaggatga
     1981 cgaagggaaa ggtaatgcac cagcaatgaa ttgcatggtc tattatcatc ggattggcga
     2041 ggatcaggag agtgacgtcc ttgttcatga ggatcctgag catcctttct ggatctcgtc
     2101 ggtgcagctc actccgagcg gccgatacat cctctttgcc gcgagccgcg acgcaagtca
     2161 cactcaatta gtcaagatcg cagatcttca cgaaaatgac attgggacga atatgaaatg
     2221 gaaaaacctt catgatccat gggaggctag gtttacaata gtcggagacg agggttcaaa
     2281 gatctatttc atgaccaatc tcaaggccaa gaactacaaa gtggcgacgt ttgatgccaa
     2341 ccacccagac gagggtctta cgacgctcat agcagaagac cctaacgcgt ttctcgtttc
     2401 agcaagcatt cacgcacaag acaagctcct acttgtttat cttcgtaacg ccagccacga
     2461 gattcatatc cgagatctaa ccactgggaa gcccctcggg cgtatctttg aggatttact
     2521 tggacagttt atggtatcag gtcgacggca ggataacgat attttcgttc tctttagcag
     2581 tttcctctct cctggaactg tgtatcggta cacgttcggt gaagagaaag gataccgttc
     2641 actatttcgt gctataagta ttccgggcct caatctagat gactttatga cagagtcggt
     2701 tttctatcca tccaaggacg ggacttctgt tcacatgttt atcacccgcc cgaaggatgt
     2761 actgctcgat ggaacttccc ctgtcctaca atacggctat ggcggtttct ccctagcaat
     2821 gcttcctaca ttctccctct ctacgctgct attctgcaaa atctacaggg caatctacgc
     2881 gatacccaat attcgtggtg gttcggaata cggcgagtca tggcaccggg agggtatgtt
     2941 agacaagaag cagaacgtgt ttgacgactt caacgctgca accgaatggc ttattgcgaa
     3001 taagtacgca agcaaggatc gcattgccat ccgaggagga tcaaatggag gtgttttgac
     3061 cactgcatgt gcaaatcaag caccgggact ctaccgttgt gtgatcacaa ttgagggtat
     3121 aattgatatg cttagatttc ctaagtttac atttggcgct agctggcgtt ctgaatatgg
     3181 cgatcctgag gatcctgaag atttcgactt catattcaaa tactccccct atcacaacat
     3241 ccctcccccg ggagacactg tcatgccagc catgctattc tttactgcgg cgtatgatga
     3301 tcgtgtctcg cctttgcaca cgttcaagca cgttgcggca ttgcaacaca actttcccaa
     3361 aggcccgaat ccatgcctaa tgcgcatcga cttgaattcg ggacactttg ctggcaagag
     3421 tacgcaggag atgttggaag aaacggcaga tgaatacagc tttatcggga agtctatggg
     3481 cctcactatg cagactcaag gttcagtgga ttcaagccgc tggtcctgcg taacagttta
     3541 agcggccgcg aattaattcg ccttagacat gactgttcct cagttcaagt tgggcactta
     3601 cgagaagacc ggtcttgcta gattctaatc aagaggatgt cagaatgcca tttgcctgag
     3661 agatgcaggc ttcatttttg atactttttt atttgtaacc tatatagtat aggatttttt
     3721 ttgtcatttt gtttcttctc gtacgagctt gctcctgatc agcctatctc gcagctgatg
     3781 aatatcttgt ggtaggggtt tgggaaaatc attcgagttt gatgtttttc ttggtatttc
     3841 ccactcctct tcagagtaca gaagattaag tgagacgttc gtttgtgcaa gcttatcgat
     3901 aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg
     3961 aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata
     4021 ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc cgacagcatc
     4081 gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct atgcgcaccc
     4141 gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc ttcgctactt
     4201 ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat ctatcgaatc
     4261 taaatgtaag ttaaaatctc taaataatta aataagtccc agtttctcca tacgaacctt
     4321 aacagcattg cggtgagcat ctagaccttc aacagcagcc agatccatca ctgcttggcc
     4381 aatatgtttc agtccctcag gagttacgtc ttgtgaagtg atgaacttct ggaaggttgc
     4441 agtgttaact ccgctgtatt gacgggcata tccgtacgtt ggcaaagtgt ggttggtacc
     4501 ggaggagtaa tctccacaac tctctggaga gtaggcacca acaaacacag atccagcgtg
     4561 ttgtacttga tcaacataag aagaagcatt ctcgatttgc aggatcaagt gttcaggagc
     4621 gtactgattg gacatttcca aagcctgctc gtaggttgca accgataggg ttgtagagtg
     4681 tgcaatacac ttgcgtacaa tttcaaccct tggcaactgc acagcttggt tgtgaacagc
     4741 atcttcaatt ctggcaagct ccttgtctgt catatcgaca gccaacagaa tcacctggga
     4801 atcaatacca tgttcagctt gagacagaag gtctgaggca acgaaatctg gatcagcgta
     4861 tttatcagca ataactagaa cttcagaagg cccagcaggc atgtcaatac tacacagggc
     4921 tgatgtgtca ttttgaacca tcatcttggc agcagtaacg aactggtttc ctggaccaaa
     4981 tattttgtca cacttaggaa cagtttctgt tccgtaagcc atagcagcta ctgcctgggc
     5041 gcctcctgct agcacgatac acttagcacc aaccttgtgg gcaacgtaga tgacttctgg
     5101 ggtaagggta ccatccttct taggtggaga tgcaaaaaca atttctttgc aaccagcaac
     5161 tttggcagga acacccagca tcagggaagt ggaaggcaga attgcggttc caccaggaat
     5221 atagaggcca actttctcaa taggtcttgc aaaacgagag cagactacac cagggcaagt
     5281 ctcaacttgc aacgtctccg ttagttgagc ttcatggaat ttcctgacgt tatctataga
     5341 gagatcaatg gctctcttaa cgttatctgg caattgcata agttcctctg ggaaaggagc
     5401 ttctaacaca ggtgtcttca aagcgactcc atcaaacttg gcagttagtt ctaaaagggc
     5461 tttgtcacca ttttgacgaa cattgtcgac aattggtttg actaattcca taatctgttc
     5521 cgttttctgg ataggacgac gaagggcatc ttcaatttct tgtgaggagg ccttagaaac
     5581 gtcaattttg cacaattcaa tacgaccttc agaagggact tctttaggtt tggattcttc
     5641 tttaggttgt tccttggtgt atcctggctt ggcatctcct ttccttctag tgacctttag
     5701 ggacttcata tccaggtttc tctccacctc gtccaacgtc acaccgtact tggcacatct
     5761 aactaatgca aaataaaata agtcagcaca ttcccaggct atatcttcct tggatttagc
     5821 ttctgcaagt tcatcagctt cctccctaat tttagcgttc aacaaaactt cgtcgtcaaa
     5881 taaccgtttg gtataagaac cttctggagc attgctctta cgatcccaca aggtggcttc
     5941 catggctcta agaccctttg attggccaaa acaggaagtg cgttccaagt gacagaaacc
     6001 aacacctgtt tgttcaacca caaatttcaa gcagtctcca tcacaatcca attcgatacc
     6061 cagcaacttt tgagttgctc cagatgtagc acctttatac cacaaaccgt gacgacgaga
     6121 ttggtagact ccagtttgtg tccttatagc ctccggaata gactttttgg acgagtacac
     6181 caggcccaac gagtaattag aagagtcagc caccaaagta gtgaatagac catcggggcg
     6241 gtcagtagtc aaagacgcca acaaaatttc actgacaggg aactttttga catcttcaga
     6301 aagttcgtat tcagtagtca attgccgagc atcaataatg gggattatac cagaagcaac
     6361 agtggaagtc acatctacca actttgcggt ctcagaaaaa gcataaacag ttctactacc
     6421 gccattagtg aaacttttca aatcgcccag tggagaagaa aaaggcacag cgatactagc
     6481 attagcgggc aaggatgcaa ctttatcaac cagggtccta tagataaccc tagcgcctgg
     6541 gatcatcctt tggacaactc tttctgccaa atctaggtcc aaaatcactt cattgatacc
     6601 attattgtac aacttgagca agttgtcgat cagctcctca aattggtcct ctgtaacgga
     6661 tgactcaact tgcacattaa cttgaagctc agtcgattga gtgaacttga tcaggttgtg
     6721 cagctggtca gcagcatagg gaaacacggc ttttcctacc aaactcaagg aattatcaaa
     6781 ctctgcaaca cttgcgtatg caggtagcaa gggaaatgtc atacttgaag tcggacagtg
     6841 agtgtagtct tgagaaattc tgaagccgta tttttattat cagtgagtca gtcatcagga
     6901 gatcctctac gccggacgca tcgtggccga cctgcagggg ggggggggcg ctgaggtctg
     6961 cctcgtgaag aaggtgttgc tgactcatac caggcctgaa tcgccccatc atccagccag
     7021 aaagtgaggg agccacggtt gatgagagct ttgttgtagg tggaccagtt ggtgattttg
     7081 aacttttgct ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc
     7141 aactcagcaa aagttcgatt tattcaacaa agccgccgtc ccgtcaagtc agcgtaatgc
     7201 tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat
     7261 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     7321 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     7381 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     7441 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct
     7501 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     7561 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     7621 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     7681 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     7741 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     7801 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     7861 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     7921 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     7981 ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa
     8041 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     8101 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt
     8161 cccccccccc cctgcaggtc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
     8221 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     8281 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     8341 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     8401 taatgcagga gtcgcataag ggagagcgtc gagtatctat gattggaagt atgggaatgg
     8461 tgatacccgc attcttcagt gtcttgaggt ctcctatcag attatgccca actaaagcaa
     8521 ccggaggagg agatttcatg gtaaatttct ctgacttttg gtcatcagta gactcgaact
     8581 gtgagactat ctcggttatg acagcagaaa tgtccttctt ggagacagta aatgaagtcc
     8641 caccaataaa gaaatccttg ttatcaggaa caaacttctt gtttcgaact ttttcggtgc
     8701 cttgaactat aaaatgtaga gtggatatgt cgggtaggaa tggagcgggc aaatgcttac
     8761 cttctggacc ttcaagaggt atgtagggtt tgtagatact gatgccaact tcagtgacaa
     8821 cgttgctatt tcgttcaaac cattccgaat ccagagaaat caaagttgtt tgtctactat
     8881 tgatccaagc cagtgcggtc ttgaaactga caatagtgtg ctcgtgtttt gaggtcatct
     8941 ttgtatgaat aaatctagtc tttgatctaa ataatcttga cgagccagac gataatacca
     9001 atctaaactc tttaaacgtt aaaggacaag tatgtctgcc tgtattaaac cccaaatcag
     9061 ctcgtagtct gatcctcatc aacttgaggg gcactatctt gttttagaga aatttgcgga
     9121 gatgcgatat cgagaaaaag gtacgctgat tttaaacgtg aaatttatct caagatctgc
     9181 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     9241 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     9301 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     9361 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     9421 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     9481 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9541 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9601 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9661 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9721 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9781 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9841 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9901 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9961 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10021 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10081 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10141 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10201 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10261 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10321 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10381 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10441 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10501 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10561 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10621 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10681 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
    10741 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10801 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10861 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10921 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10981 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
    11041 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    11101 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11161 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11221 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11281 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11341 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    11401 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    11461 ttcgtcttca agaattaatt ctcatgtttg acagcttatc atcgataagc tgactcatgt
    11521 tggtattgtg aaatagacgc agatcgggaa cactgaaaaa taacagttat tattcg
//
